We have stressed the need to have young mated queens available at various times during the active season: in the spring to replace a 'poor queen' and in the swarming season to replace queens in colonies that have made up their minds to swarm. Also at those times all through the active season from April to the end of July when queens may suddenly fail and need replacing, and at the end of the season when two-year-old queens should be replaced with young ones. In other words, for many reasons the useful length of life of queens may vary considerably and some preparation must be made to provide replacements which are of good quality and breeding.
With the honeybee there is a more obvious difference between the concepts of quality and breeding than with many other animals. The quality of a good queen with excellent inheritance can be heavily concealed by poor nurture during her larval development. Bee breeding is difficult and although extremely interesting may have to be left to the beekeeper with a large number of colonies. Queen rearing, on the other hand, can and should be practised by all beekeepers. The queens that are to be used in the apiary should be the product of thought and planning. They should not be the queens that the colony happens to make, when it can no longer hold together with the queen it has.
We know that the fertilized egg of the honeybee can be turned into either a worker or a queen dependent upon how it is housed and fed. We also know from experience and research that the best queens are those produced in large colonies where there are lots of young bees and plenty of pollen for them to feed on when they are making 'bee milk'. The queen larvae are then fed to a maximum and grow large, and with a large number of egg tubes in their ovaries. In contrast, the small nucleus will never be able to produce a top-line queen. T h e nucleus is usually struggling to build up and has as many worker larvae mouths to
feed as it can manage. To expect a number of queen cells as well is to ask for a poorly-fed queen.
T h e best queens are produced from very young larvae, or eggs. Research shows that the larva which is treated as a queen from the start produces the heaviest queens with the highest number of egg tubes and the largest spermatheca. It also shows that the reduction in these factors occurs as progressively older larvae are taken for queen rearing. Thus if we are to produce queens for use in our own apiary we should produce them in as large a colony as possible: one in which there are plenty of young bees to act as nurses, and ample pollen. Finally, the worker larvae from which the queens are to be made should be as young as possible when they are started off on their careers as queens. The process of queen rearing can be broken down into four separate parts: the provision of the colony which is to produce the queens, usually called the cell-building colony; the. selection of a colony which is to provide the larvae—this is the breeder colony and it contains the breeder queen; the process of giving the larvae from the breeder queen to the cell-building colony, and finally the removal of the ripe queen cells from the cell-building colony before the first virgin queen hatches (or she will kill all the rest) and the placing of these queen cells into small 'mating nuclei' from which they can fly, mate, and in which they can start laying.
Let us look first of all at a selection of breeding stock. It would be stupid not to take advantage of the process of queen production to increase the value of our stock as much as possible. Bad characteristics which can easily be recognized can be bred out very quickly, and these include stinging, following and excessive running about on the comb when being manipulated, all separately inherited and tiresome. Running about on the comb can be so bad that when combs are lifted from the hive the bees on them run down to the bottom of the combs, form clusters and drop off. It requires little imagination to picture the problem if this is happening when you are looking for the queen. These characteristics should be culled from your strain of bee as quickly as possible by avoiding producing queens from colonies which show them, and by replacing the queen in such colonies as soon as possible. The sooner they are gone the better, because all the while they are there they will be producing drones which may mate with the young queens and pass the bad traits on to future generations.
Persistent swarming is another inherited trait that can be reduced by culling—that is by replacing those queens whose colonies show it. Swarming is the bees' natural method of increasing the number of colonies, or the number of sexual females, whichever way you wish to look at it. Without swarming reproduction does not take place, and from the point of view of the species as a whole this would reduce its ability to withstand adverse conditions. I therefore feel that it is not
possible to envisage a useful bee from which the swarming instinct has been entirely eliminated. It can, however, be greatly reduced, and for this reason I would try to breed from bees which neither try to swarm every season, nor make large numbers of queen cells when they do. I would breed from colonies that once having made up their mind build up to nine or ten cells, but colonies such as one I had in Devon which produced 153 queens and queen cells at one time should be culled as rapidly as possible.
Breeding for honey production is much more difficult because its characteristics cannot be assessed in any meaningful way. Individual colonies which produce very large surpluses of honey may do so for many reasons other than the inheritance of a very high work rate. They may just be very good robbers, and have stolen their honey from other colonies. They may be in a position in the apiary where a lot of bees drift in on a prevailing wind. They may always be that truly exceptional case which has inherited genes which all add together to give a very high production, but this is a fortuitous happening which is not possible to repeat in the offspring. The only useful method is to look at the family from which a queen comes before she is chosen as a breeder. Her sisters should all be equally good and all their colonies acceptable to the beekeeper.
You often hear it said that you should not breed from the exceptional colony. But often this is then altered to 'you should not breed from your best colony', which is not necessarily correct. If you have only three or four colonies, or even a dozen, you are unlikely to have an 'exceptional' colony in your apiary. These are by definition very rare and the chances of their turning up amongst a few hives is very small. The beekeeper with just a few hives is best advised to breed from his best colony. He may come unstuck once or twice in a lifetime but this is a chance worth taking. If he has a large number of colonies then he is best advised to breed from a queen belonging to a good family. The beekeeper with the small number of hives can of course band together with a number of other beekeepers and by selecting over all their colonies practise 'family selection'—the result will be much more successful in the long run than working alone. So much for breeder colony selection.
The provision of the cell-building colony will depend very much on the number of hives you are catering for and the number of queens you wish to produce. I will therefore deal with the subject at three different levels. One for the beekeeper with up to about ten colonies, secondly for the man with up to fifty colonies, and finally large-scale rearers. The small scale beekeeper will do best by deciding to work his best colony on two brood chambers. This is the colony that is building up most rapidly in the early season. If this colony is given a second brood chamber of drawn combs the bees should spread up into it very
rapidly. If the colony which is to be given the second brood chamber has an arch of honey in the top of the frames of the original brood chamber, get them to shift this by scraping the capping with the hook of your hive tool, thus laying bare the honey. The bees will usually then remove the honey and take it to the top box. The colony should be built up rapidly to as large a size as possible by the third week of May. This date will depend upon the time at which you can safely start queen rearing and expect to get the queens mated.
Once the colony is using most of the brood chambers add supers as required: by the third week of May it should have at least one super, if not two. The colony is now ready to produce queen cells, and you should act as follows. First find the queen and place her in a match box with a few bees to look after her. The two brood chambers can then be sorted through. Put all the unsealed brood in one and make the box up with sealed brood and one good frame of pollen if this available. Put the sealed brood on the flanks—i.e. nearest the hive walls—the unsealed brood between them and the pollen comb in the centre. The remainder of the combs are put in the other brood chamber, the queen freed on to one of them, and the brood chamber then replaced on the floor on its original site. The supers are put on next above a queen excluder, and if there is only one super at this time I would add a second. A second queen excluder should now be placed above the supers and the brood chamber containing the young brood placed on top covered by the usual crown board and roof.
The young nurse bees will be drawn to the top brood chamber by the presence of the unsealed brood, but the fact that they are isolated from the queen by two excluders and two supers and that the full transfer of food, and hence queen substance, will not take place between the bottom and top brood chamber bees, means they will usually make a small number of queen cells. In this case you are using this colony as the breeder colony as well as for the production of cells. It may be, however, that the queen you would like to breed from is not capable of building up a colony large enough for the above procedure. In this case you must insert a marked frame of eggs and very young brood taken from the colony from which you wish to breed your queens, and remove any queen cells produced from their own brood by the bees in the queen-rearing colony. If you are lucky with this method and get a satisfactory number of cells, as soon as these are ripe—about ten days after the colony is split as described—you may either cut them out and distribute them to mating nuclei, or the top brood chamber combs and bees can themselves be split up into mating nuclei using some of the queens. You will only be able to split it into about three nuclei if you are going to have sufficient bees in each.
The second method is for the production of from twenty to forty queens and is much more positive than the above method. In the early
season the technique is the same—a very large colony is built up on two brood chambers. Because the beekeeper has a greater number of colonies he can take frames of brood from colonies which are building up well and give these to the cell-building colony, thus building it up to a massive size. When the time for queen rearing comes the queen is found and removed on to a small two-frame nucleus. The rest of the colony is made up with a super at the bottom, on the floor, then a queen excluder with a brood chamber above it. The brood chamber should be filled with eight frames of sealed brood, one frame of unsealed brood and a frame of pollen, the unsealed brood and pollen being placed in the centre of the brood chamber. Assuming an eleven frame chamber this leaves an empty place which can be filled with a dummy board. The colony should now be given all the bees from the rest of the combs, both brood and super. This is done by shaking them into the brood chamber just set up, and the colony may then be given a feeder of syrup and closed down. Any surplus brood and supers should be dispersed amongst the other colonies—as these frames have no bees they cannot be given to their own queen as she has not enough workers.
The main colony, now congested to overflowing and queenless, will make queen cells. I would leave the colony for two or three days and then remove the new queen cells in it, shaking the bees off the combs so that none is missed, and give them a frame of larvae which are to be turned into the queens we want from the breeder queen. These larvae should be put in the centre of the broodnest between the frame of young brood and the frame of pollen. Queen cells will be constructed on this frame and will be sealed in four days, so a second batch of larvae from the breeder queen can be given at this time if required. Cells will be ready to be distributed ten days after the larvae are put in, so if two batches are required the colony will be cell building for 17-18 days from the time the queen was removed. As soon as their role of cell- building colony is completed, the original queen in her nucleus can be put back and the colony brought back into honey production.
The final method is for the beekeeper who requires a considerable number of queens. It is very like the last method but involves combining two large colonies in a special brood chamber that takes 13-15 frames. This massive colony is kept going from about the third week in May to the end of July, and larvae for queen rearing are placed in every three or four days. As the worker brood hatches, more is added from other colonies and this prevents a fall in population. Such a method can produce several hundred queens in the course of a summer but requires a back-up of mating nuclei available in the required quantity.
Having set up this cell-building colony, two or three days later the whole colony is looked through and, as with method 2, any queen cells are destroyed, and larvae from the breeder colony inserted.
Grafting
Insertion of larvae can be done in many ways but my own preference is for the 'Doolittle' method or, as it is commonly termed, 'grafting'. This is the process whereby a number of small waxen cups are made by the beekeeper. These are attached to bars constructed in the usual size frame. Small larvae are then transferred from their comb in the breeder colony, one into each cup. These are then placed in the cell- building colony for the queenless bees to turn into queens. I find this method the easiest, quickest, least messy and most reliable of all the methods generally used. This is how it is done.
The wax cups are prepared by dipping a wooden or glass former into molten beeswax. The former can be made from 1/4 inch dowelling or glass tubing, the ends of which are rounded off and well smoothed. If a lot of cells are to be made a bar with a number of formers can be dipped, giving several cups for each dipping, as drawn above. The formers will stick to the wax unless they are wet, and for this reason they are placed in water several minutes before use and are dipped in again between each application. Wax is melted in a small water bath—nothing more elaborate than a small empty meat or fruit tin standing in an old saucepan is needed, though a special double- jacketed trough as shown in fig. 38 can be used. The wax should be good, clean wax and should not be heated much above melting point. The former is then removed from the water, shaken to get rid of excess
water and dipped about five times into the molten wax to a depth of about 5/16 inch. Some beekeepers try to dip progressively less deeply each time to provide a thin edge to the 'cell'. I have never found that this helps in any way and usually dip to the same level using a depth guide on the former. The single former is dipped until the wooden crosspiece hits the sides of the container and the multiple former has the two bolts at the end which can be adjusted for height and which contact the sides of the trough. After dipping, the former is placed back in the water for a few seconds, when the cups can easily be twisted off the ends.
The cells are fastened to bars on a frame made up as illustrated