Chapter 6 The presence of Intracellular bacteria inbladder epithelium of patients
6.3 Method development and results
6.3.10 Results of sensitivity tests
Tables 6.9, 6.10 and 6.11 show the sensitivity of the bacterial isolates to each combination of antibiotic and enzymes. There was no growth in the culture medium alone but there was growth in every well which contained growth medium with bacterial isolates alone. Culture confirmed the optical densities OD600of 0.5 to correspond to a bacterial count of 1x108cfu/ml, OD600of 0.05 corresponding to 1x106cfu/ml and the bacterial suspension diluted fourfold to 1x103cfu/ml in each case.
The tables show that all bacterial isolates were sensitive to Gentamicin at a concentration of 200µg/ml except Enterococcus.faecalis at 1x108cfu/ml, which was sensitive to Linezolid. E coli was resistant to Amoxicillin 200µg/ml at all bacterial concentrations. Streptococcus.anginosus and Proteus. mirabilis were resistant to Amoxicillin 200µg/ml at bacterial concentrations of
1x108cfu/ml and 1x106cfu/ml. Lactobacillus. gasseri, Corynebacterium.Sp were sensitive to all antibiotics. The addition of enzymes did not improve the sensitivity of the bacterial isolates to the antibiotics at any concentration of bacteria.
Bacteria 1x108cfu/ml
E.coli E.faecalis Strep.anginosus Corynebacteriu m. Sp. Lactobacillus. gasseri Proteus. mirabilus Consistency of media
Media+bacteria Growth Growth Growth Growth Growth Growth
G No
growth
Growth No growth No growth No growth No growth
L Growth faint
growth
No growth No growth No growth Growth
A Growth Growth Growth No growth No growth Growth
GLA No
growth
No growth No growth No growth No growth No growth
GL No
growth
No growth No growth No growth No growth No growth
GA No
growth
Growth No growth No growth No growth No growth
AL Growth faint
Growth
No growth No growth No growth Growth
GE No
growth
Growth No growth No growth No growth No growth
LE Growth faint
growth
No growth No growth No growth Growth
AE Growth Growth Growth No growth No growth Growth
GLAE No
growth
No growth No growth No growth No growth No growth
GLE No
growth
No growth No growth No growth No growth No growth
GAE No
growth
Growth No growth No growth No growth No growth
ALE Growth Growth No growth No growth No growth Growth
Table 6.9. Bacterial sensitivity at bacterial count of 1x108cfu/ml to combinations of gentamicin 200µg/ml, Linezolid 200µg/ml, amoxicillin 200µg/ml and mutanalysin 50µg/ml, Lysostaphin 50µg/ml and Lysozyme 50µg/ml. G= Gentamicin, L= Linezolid, A= Amoxicillin, E= Enzymes (Lysostaphin, Lysozyme, mutanalysin)
Bacteria 1x106cfu/ml
E.coli E.faecalis Strep.anginosus Corynebacteriu m. Sp Lactobacillus. gasseri Proteus.mirabilus Consistency of media
Media+bacteria Growth Growth Growth Growth Growth Growth
G No
growth
No growth No growth No growth No growth No growth
L Growth No growth No growth No growth No growth Growth
A Growth No growth Growth No growth No growth Growth
GLA No
growth
No growth No growth No growth No growth No growth
GL No
growth
No growth No growth No growth No growth No growth
GA No
growth
No growth No growth No growth No growth No growth
AL Growth No growth No growth No growth No growth Growth
GE No
growth
No growth No growth No growth No growth No growth
LE Growth No growth No growth No growth No growth Growth
AE Growth No growth Growth No growth No growth Growth
GLAE No
growth
No growth No growth No growth No growth No growth
GLE No
growth
No growth No growth No growth No growth No growth
GAE No
growth
No growth No growth No growth No growth No growth
ALE Growth No growth No growth No growth No growth Growth
Table 6.10. Bacterial sensitivity at bacterial count of 1x106cfu/ml to combinations of gentamicin 200µg/ml, Linezolid 200µg/ml, amoxicillin 200µg/ml and mutanalysin 50µg/ml, Lysostaphin 50µg/ml and Lysozyme 50µg/ml.G= Gentamicin, L= Linezolid, A= Amoxicillin, E= Enzymes (Lysostaphin, Lysozyme, mutanalysin)
Bacteria 1x103cfu/ml
E.coli E.faecalis Strep.anginosus Corynebacteriu m. Sp Lactobacillus. gasseri Proteus.mirabilus Consistency of media
Media+bacteria Growth Growth Growth Growth Growth Growth
G No
growth
No growth No growth No growth No growth No growth
L faint
Growth
No growth No growth No growth No growth No growth
A Growth No growth No growth No growth No growth No growth
GLA No
growth
No growth No growth No growth No growth No growth
GL No
growth
No growth No growth No growth No growth No growth
GA No
growth
No growth No growth No growth No growth No growth
AL Growth No growth No growth No growth No growth No growth
GE No
growth
No growth No growth No growth No growth No growth
LE Growth No growth No growth No growth No growth No growth
AE Growth No growth No growth No growth No growth No growth
GLAE No
growth
No growth No growth No growth No growth No growth
GLE No
growth
No growth No growth No growth No growth No growth
GAE No No growth No growth No growth No growth No growth
Table 6.11. Bacterial sensitivity at bacterial count of 1x103cfu/ml to combinations of gentamicin 200µg/ml, Linezolid 200µg/ml, amoxicillin 200µg/ml and mutanalysin 50µg/ml, Lysostaphin 50µg/ml and Lysozyme 50µg/ml.G= Gentamicin, L= Linezolid, A= Amoxicillin, E= Enzymes (Lysostaphin, Lysozyme, mutanalysin)