with HSV-tk suicide genes. Excellent glioma homing and high herpes simplex virus 691
thymidine kinase (HSV-tk) expression enables an efficient convert of ganciclovir 692
(GCV) to its toxic metabolite of GCV-triphosphate (GCV-3P) at the glioma sites. In 693
addition, the overexpression of Connexin 43 (Cx43) triggered by MFIONs promotes 694
the gap junction intercellular communication (GJIC) between MSCs and glioma cells, 695
thereby enhancing the intercellular transfer of GCV-3P toward glioma cells and 696
resulting in a significant bystander cell death of glioma cells.
697 698 699
Supplementary Material 700
Supplementary figures and tables 701
702
Acknowledgements 703
We would like to thank Mr. Weitong Hu from the school of Life science and medicine, 704
King’s College London for English writing assistance. We appreciate the technical 705
supports by Morphological Platform, Zhejiang University School of Medicine. This 706
research was financial supported by the National Natural Science Foundation of China 707
(Nos. 81620108028, 81703423, 81973252), the China Postdoctoral Science Foundation 708
(2018M640568), and the Ten-thousand Talents Program of Zhejiang Province 709
performed all experiments; TH, YS and XJ assisted with the cell experiments; RL, JM, 714
HS and HW assisted with the animal studies and the safety evaluations; DX conducted 715
the anti-glioma studies; HC harvested human placental mesenchymal stem cells and 716
conducted the stem cell experiments; XS prepared the glioma animal model; DL 717
prepared the magnetic iron nanoparticles; AL and TZ analysed the data; AL, TZ and JG 718
The authors have declared no competing interests.
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