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We are interested studying the effects of sleep loss on attention and emotion. In this study, some people will be asked to restrict their sleep to 4 hours on one night, while others will be asked to sleep for 8 hours. Participants will then be asked to spend one afternoon in the Sleep Research Laboratory where EEG will be recorded to measure brain activity while performing various computer tasks of attention and emotion.

Here are the details of what would be expected of you:

1. First, we will get some information from you on the phone today to see if you meet our basic criteria for participation.

2. If you are suitable for the study, we will then ask you to attend a 1 1/2-hr ORIENTATION session where you will tour the Sleep Lab, and be given a full consent form – this provides details about the study – and you can decide whether or not you are interested in full participation at that time. You will then complete a series of questionnaires, have your heart rate recorded, and practice the computerized cognitive tasks that we will use in the main study. The questionnaires will ask questions about your physical and mental health, sleep habits and personality. These questionnaires will confirm that you meet all of the eligibility requirements for this study.

3. If you wish to participate, you will then be asked to complete a sleep/wake diary from home each morning and wear an Activity Monitor (that looks like a wrist watch) for a period of one week prior to participation in the study. You will be asked to keep a regular sleep/wake schedule that week, sleeping for about 8 hrs each night from approximately 11pm-7am (or midnight-8am).

If you are deemed ineligible or if you choose not to participate in the laboratory study, there will be no compensation for the pre-study screening (that is, the orientation session, and sleep diaries/activity monitor for the week prior to the main study day). If you do not complete the main part of the study for whatever reason, all of the information you provided in pre-study screening will be destroyed.

4. For the main part of the study, you will be scheduled to spend one afternoon in the Sleep Laboratory, from 1-6pm for a performance assessment. You will have your brain activity recorded using an electrode cap while performing computer tasks. You will also be asked to provide saliva samples at various times by spitting into a test tube because we are measuring hormones. Also, you may or may not be asked to restrict your sleep on the prior night.

Are you interested? [yes] – Ok, I have a few questions for you to make sure you are suitable for the study. If you are the type of person we are looking for, we will schedule your orientation appointment.

II. INCLUSION CRITERIA:

First, do you think you would have any difficulty keeping a regular sleep schedule for the one week before the lab study, that is, sleeping about 8 hrs each night and going to bed from about 11pm-7am or midnight-8am (i.e., not staying up really late or sleeping in on any of the days for a week)? -

_______________________________________________________________________ What days would you be free to participate from about 1-6pm (indicate schedule):

________________________________________________________________________ Age (17-30): ____________

Weight (indicate kg or lbs): __________ Gender: M / F

If female - do you have a “regular” menstrual cycle, and do you take any

hormones for contraceptive purposes or to regulate your cycle (e.g., birth control pills, patches or injections)? [yes regular; no use of hormones -Must have not used for 2 months and now have regular cycle]: ________________________________

Smoker: Y / N [no]

Handedness: R / L [right]

How many caffeinated drinks do you typically have in a day [min - moderate, <3]: ____ Is English your first language (if not, did you learn before age 8 or describe fluency): ___ Do you have any difficulties with vision [OK with glasses/contacts], or hearing [no, in both ears]: _____________

III. Questions on SLEEP:

1. Do you consider yourself to be a good sleeper? [yes]: ___________________

2. What are your usual sleeping times [approx 23:00-07:00]:________________ 3. How does this change on weekends? [sleeping-in a bit is ok] _____________ 4. Do you have difficulty falling asleep at night [no]: _____________________

5. Do you wake up often during the night and are unable to return to sleep [no]:______ 6. Have you ever been diagnosed with a Sleep Disorder [no]:___________

7. Have you ever been told you kick your legs all-night long or stop breathing during the night? [no] ________________________

8. Do you experience restless legs or a “creepy crawling” sensation before bed each night? [no] ________________

9. Would you describe yourself as excessively tired during the day [no]: ____________ 10. Do you currently work shift work [no]; any history of shiftwork? ______________ 11. Do you take daytime naps? Y / N

How frequently (# / week) _________ Duration for each ______________ 12. Have you ever pulled an all-nighter? How often/how many times etc?

RSA, EMOTION PROCESSING AND SLEEP 124

IV. Questions on HEALTH:

1. Are you presently in good health [yes]: ______________________________

2. Taking any medications [no]: ______________________________________ 3. Any history of depression, anxiety or schizophrenia [no]:________________

4. Any history of head injury (e.g., car accident, stroke, loss of consciousness), epilepsy,

or other neurological condition [no]: ___________________________________

5. Any history of chronic pain [no]: ____________________________________ 6. Any history of heart disease or cardiac abnormalities [no]: ________________

Appendix E

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Title of Study: Impact of Sleep Restriction on Attention and Emotion

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