expression in each pairwise comparison on Clone 8+ cell lines
Cell Line SAM Significant genes SAM Significant genes FDR
Pairwise Comparison Increased Decreased % YCl.8+ vs YCl.8+ +E 1746 3363 45%
YCl.8+ vs OCl.8+ 2219 2927 45%
YCl.8+ vs Cl.8R 2754 2408 63%
YCl.8+ +E vs OCl.8+ +E 0 0 N/A
YCl.8+ +E vs Cl.8R +E 1800 2283 45% OCl.8+ vs OCl.8+ +E 2194 2735 52% OCl.8+ vs ZfeCl.8+ 2258 2464 33% OCl.8+ +E vs ZfeCl.8+ +E 2005 2870 33% Cl.8R vs Cl.8R +E 0 0 N/A ZfeCl.8+ vs ZfeCl.8+ +E 2564 2437 51%
Table 6.2: Number of genes identified bySAMincreased two fold or more Cell Line SAM Significant genes SAM Significant genes FDR
Pairwise Comparison Increased Decreased %
YCl.8+ vs YCl.8+ +E 6 2 <1%
YCl.8+ vs OCl.8+ 1 10 <1%
YCl.8+ vs Cl.8R 7 2 <1%
YCl.8 + +E vs OCl.8 +E 0 0 N/A
YCl.8 + E vs Cl.8R +E 8 12 <1%
OCl.8+ vs OCl.8 + E 7 4 <1%
OCl.8+ vs ZfeCl.8+ 4 0 <1%
OCl.8+ +E vs ZfeCl.8+ +E 2 3 <1%
Cl.8R vs Cl.8R +E 0 0 N/A
ZfeCl.8+ vs ZfeCl.8+ +E 0 0 N/A
Table 6.2: The number of genes revealed bySAMas expressed with a threshold of two fold or more in the Clone 8+ cell line hybridisations. No genes were found to be significant in 3 pairwise hybridisations. The data is listed in accordance with the ‘Flychip’ excel data files.
FDR=False discovery rate vs =hybridised with. +E= after ecdysone.
Table 6.3a:Genes increased two fold or more in Cl.8R cells
Gene Gene product: location and role
CG10126 Calcium ion binding
CG1090 Calcium, potassium:sodium antiporter
Cg25C Basement membrane, collagen type IV,
Gel Actin filament, cytosol, extracellular
Idgf3 Extracellular, imaginal disc growth factor ,
Pxn Extracellular matrix, peroxidase
regucalcin Calcium ion binding
Table 6.3a:Genes increased two fold or more in Cl.8R cells
Genes identified by analysis withSAMwith an increased expression in Clone 8R cells when compared to Young Clone 8+ cells are listed. 7 genes were identified which were increased two fold or more.
Table 6.3b:Genes decreased two fold or more in Cl.8R cells
Gene Gene product: location and role
CG15828 Extracellular space, lipid binding
Imp-L2 Extracellular, cell adhesion molecule
Table 6.3b:Genes decreased two fold or more in Cl.8R cells
Genes identified by analysis withSAMwith a decreased expression in Clone 8R cells when compared to Young Clone 8+ cells are listed. 2 genes were identified which were decreased two fold or more.
Table 6.4a:Genes increased two fold or more in Cl.8R cells after ecdysone
Gene Gene product: location and role
Cg25C Basement membrane, collagen type IV,
CG9089 Not known
Chd64 Actin binding
Chit Extracellular, chitinase
ERR Ligand dependent nuclear receptor
Idgf2 Extracellular, imaginal disc growth factor, NOT chitinase
Inos Inositol3phosphate synthase
Pxn Extracellular matrix, peroxidase
Table 6.4a:Genes increased two fold or more in Cl.8R cells after ecdysone. Genes identified by analysis withSAMwhich were increased in Clone 8R cells after ecdysone when compared to Young Clone 8+ cells after ecdysone are listed. 8 genes were identified which were increased two fold or more.
Table 6.4b: Genes decreased two fold or more in Cl.8R cells after ecdysone Gene Gene product: location and role
CG14749 poly(A)+ mRNAnucleus export
fax Axonogenesis
GstS1 Cellular component unknown, Glutathione peroxidase
Hsp27 Heat shock protein, AGE
ImpL2 Extracellular, cell adhesion molecule
poe Calmodulin binding AGE
regucalcin Calcium ion binding
CG31363 Not known
CG5854 Not known
CG6234 Not known
CG9213 Not known
CG7874 Not known
Table 6.4bGenes decreased two fold or more in Cl.8R cells.
Genes identified by analysis withSAMwhich were decreased in Clone 8R cells after ecdysone when compared to Young Clone 8+ cells after ecdysone are listed. 7 genes of known function were identified which were decreased two fold, 5 genes were of unknown function.
Table 6.5a:Genes increased two fold or more in Old Cl.8+ cells
Gene Gene product: location and role
CG3380 Sodium independent organic anion transporter
Table 6.5a:Genes increased two fold or more in OCl.8+ cells
Genes identified by analysis withSAMincreased in expression in Old Clone 8+ cells when compared to Young Clone 8+ cells are listed. 1 gene was
identified which was increased two fold or more.
Table 6.5b:Genes decreased two fold or more in Old Cl.8+ cells
Gene Gene product: location and role
CG5384 Ubiquitinspecific protease, protein deubiquitination
cul-2 Nuclear ubiquitin ligase complex
Doa Cytoplasm, nucleus, protein kinase, protein amino acid phosphorylation
fax Axonogenesis
Ggamma30A Cytoplasm,GTPase, Gprotein coupled receptor protein signalling pathway
CG1105 Not known
CG12505 Not known
CG7224 Not known
CG8032 Not known
CG8223 Not known
Table 6.5b:Genes decreased two fold or more in OCl.8+ cells
Genes identified by analysis withSAMwhich were decreased in Old Clone 8+ cells when compared to Young Clone 8+ cells are listed. 10 genes were
identified which were decreased two fold or more. 5 genes were reported as unknown:CG1105, CG12505, CG7224, CG8032andCG8223.
Table 6.6a:Genes increased two fold or more in ZfeCl.8+ cells
Gene Gene product: location and role
CG3074 Cathepsin B
CG3273 Actin filament reorganisation (aka scrambled (sced))
Glt Basement membrane, calcium ion binding
roX1 Dosage compensation complex
Table 6.6a:Genes increased two fold or more in ZfeCl.8+ cells
Genes identified by analysis withSAMincreased in expression in ZfeClone 8+ cells when compared to Old Clone 8+ cells are listed. 4 genes were
identified which were increased two fold or more. The geneCG3273is also known asscrambled(sced).
Table 6.7a:Genes increased two fold or more in ZfeCl.8+ cells after ecdysone
Gene Gene product: location and role
CG3074 Cathepsin B
CG3273 Not known
Table 6.7a:Genes increased two fold or more in ZfeCl.8+ cells after ecdysone Genes identified by analysis withSAMwith an increase in expression in ZfeClone8 cells after ecdysone when compared to Old Clone 8+ cells after ecdysone are listed. 2 genes were identified which were increased two fold or more.
Table 6.7b:Genes decreased two fold or more in ZfeCl.8+ cells after ecdysone
Gene Gene product: location and role
CG31305 Not known
CG7777 Water transporter
regucalcin Calcium ion binding
Table 6.7b:Genes decreased two fold or more in ZfeCl.8+ cells after ecdysone Genes identified by analysis withSAMwith a decreased expression in
ZfeClone8 cells after ecdysone when compared to Old Clone 8+ cells after ecdysone are listed. 3 genes were identified which were decreased two fold or more.
Table 6.8a:Genes increased two fold or more in YCl.8+ cells after ecdysone
Gene Gene product: location and role
CG14749 poly(A)+ mRNAnucleus export
CG31305 Not known
CG9213 Not known
fax Axonogenesis
GstS1 Cellular component unknown, glutathione peroxidase
regucalcin Calcium ion binding
Table 6.8a:Genes increased two fold or more in YCl.8+ cells after ecdysone Genes identified by analysis withSAMwith an increased expression in Young Clone 8+ cells after ecdysone when compared to Young Clone 8+ cells no ecdysone are listed. 6 genes were identified which were increased two fold or more after ecdysone.
Table 6.8b:Genes decreased two fold or more in YCl.8+ cells after ecdysone
Gene Gene product: location and role
Chd64 Actin binding
ERR Ligand dependent nuclear receptor
Table 6.8b:Genes decreased two fold or more in YCl.8+ cells after ecdysone Genes identified by analysis withSAMwith a decreased expression in Young Clone 8+ cells after ecdysone when compared to Young Clone 8+ cells no ecdysone are listed. 2 genes were identified which were decreased two fold or more after ecdysone.
Table 6.9a:Genes increased two fold or more in OCl.8+ cells after ecdysone
Gene Gene product: location and role
CG1090 Calcium, potassium:sodium antiporter
CG9213 Not known
fat-spondin Extracellular, cytoskeleton organisation
fax Axonogenesis
GstS1 Cellular component unknown, glutathione peroxidase
Inos Inositol3phosphate synthase
regucalcin Calcium ion binding
Table 6.9a:Genes increased two fold or more in OCl.8+ cells after ecdysone Genes identified by analysis withSAMin the Old Clone 8+ cells after ecdysone when compared to Old Clone 8+ cells no ecdysone, are listed. 7 genes were identified which were increased two fold or more after ecdysone.
Table 6.9b:Genes decreased two fold or more in OCl.8+ cells after ecdysone
Gene Gene product: location and role
CG10527 Farnesoic acid Omethyltransferase
CG9089 Not known
Chd64 Actin binding
ERR Ligand dependent nuclear receptor
Table 6.9b:Genes decreased two fold or more in OCl.8+ cells after ecdysone Genes identified by analysis withSAMwith a decreased expression in Old Clone 8+ cells after ecdysone when compared to Old Clone 8+ cells no
ecdysone are listed. 4 genes were identified which were decreased two fold or more after ecdysone.