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Urine Sample Processing Procedure

Signed by DCO

6.2 Urine Sample Processing Procedure

© WADA – DCO Manual - Version 3.0 – May 2011

6.2 Urine Sample Processing Procedure

Purpose

The purpose of this procedure is to describe the steps for processing a urine sample provided by an Athlete.

Actions

1. Once the Athlete has provided the required 90 mL of urine, the sample processing should begin as soon as practical. The Athlete should retain control of the collection vessel and any sample provided until the sample is sealed inside the sample collection kit bottles. The collection vessel must be in clear view of the Athlete and DCO or Chaperone at all times.

NOTE: If the volume of urine is insufficient, the Partial Sample Procedure (See

reference 6.4) should be followed.

2. The DCO should make note of the total volume of urine in the collection vessel and record it on the Doping Control Form before the sample is divided into the sample collection kit bottles.

3. If a foreign object is in the Athlete's sample, the DCO should determine whether or not the object compromises the integrity of the sample.

NOTE: If it is determined that the foreign object could compromise the integrity of the

sample (i.e. soap), the sample should be processed but an additional sample should be collected following the steps outlined in the Additional Sample Procedure (See

reference 6.5). If it is determined that the foreign object does not compromise the

integrity of the sample (i.e. blood or hair), the sample should be processed and the DCO should continue with the regular procedures for processing the sample.

4. The DCO should ask the Athlete to select and inspect a sample collection kit (a choice of at least three (3) kits is recommended) with which he/she is satisfied and in which the sample will be sealed. Each pair of A and B bottles must be secure, in tact and not tampered with prior to the selection by the Athlete.

NOTE:If the Athlete is not satisfied with the sample collection kit selected, he/she

should select another kit. If there are no other kits available and the DCO is satisfied with the remaining kit, the DCO shall instruct the Athlete to proceed with the test and should be noted on the Supplementary Report Form. If the DCO agrees with the reasons put forth by the Athlete that the sample collection kit(s) available is/are not satisfactory, the DCO shall terminate the sample collection session and this should be recorded on a Supplementary Report Form. The DCO should inform the ADO as soon as practical if this situation occurs.

6.2 Urine Sample Processing Procedure

© WADA – DCO Manual - Version 3.0 – May 2011

5. The Athlete should open the sample collection kit selected and remove the A and B bottles from its packaging. The Athlete should check that both bottles have all components in place.

6. The Athlete should check that the bottles and all other parts of the sample collection kit have the same sample code numbers (one bottle will have a prefix A and the other B). If any of the numbers do not match, then the DCO should ask the Athlete to choose another sample collection kit.

NOTE: The sample code number of any defective kits should be recorded on the

Doping Control Officer Report Form and reported to ADO. If no additional kits are available, the DCO shall terminate the test and no sample shall be secured. The DCO should inform the ADO as soon as practical if this situation occurs.

7. The DCO should ask the Athlete to read the sample code number so it can be recorded on the Doping Control Form. The DCO should also visually confirm the number before

recording it on the Form.

8. Before pouring the sample into the A & B bottles, the DCO will instruct the Athlete to leave a small amount of residual urine in the collection vessel in order to measure the specific gravity.

9. The Athlete should fill the B bottle to at least the minimum required volume indicator (30 mL).

NOTE: The B bottle must not be filled beyond the maximum fill line (i.e. if the bottle

has a shoulder) as it will be frozen at the laboratory and could break if filled beyond this level.

11.The Athlete should fill the A bottle as much as possible but at least to the minimum required volume indicator (60 mL).

NOTE: Reasonable efforts should be made to discard a minimal volume of urine. If

there is excess urine, the extra urine should be poured into the A and B bottles, with preference given to the A bottle, but not to exceed the shoulder of the bottle. If there is still excess urine after both bottles have been filled, this minimal amount of urine will be disposed of in the presence of the Athlete after the completion of the entire sample processing procedure for that Athlete.

12.The DCO should ask the Athlete to seal the sample collection kit bottles and test the security of the system to confirm they are sealed. If an Athlete has trouble during the sealing procedure, it is preferable that the Athlete’s Representative (if present) assist the Athlete. The DCO may also help if requested by the Athlete.

NOTE: If the Athlete Representative or DCO assists the Athlete with this procedure, which involves handling the Athlete's unsecured sample, it should be documented on a Supplementary Report Form.

13.The DCO should also physically verify that the bottles are properly sealed, including turning the sealed bottles upside down to check that they do not leak.

6.2 Urine Sample Processing Procedure

© WADA – DCO Manual - Version 3.0 – May 2011

14.The DCO should record the time of sealing the sample on the Doping Control Form. 15.The DCO should confirm the sample is within the required range by testing the specific

gravity using the residual volume of urine remaining in the collection vessel. The DCO should record the actual measurement for specific gravity on the Doping Control Form.

The DCO should follow the Measuring Specific Gravity procedure (See reference 6.3). 16.The DCO should request that the Athlete provide information about any medications and

other substances, including vitamins, minerals, herbs and other dietary supplements, used in the previous seven (7) days (or as requested by the ADO) and any transfusions

received in the previous six (6) months. The DCO should record the information on the Doping Control Form. If the Athlete has no substances to declare, theDCOshould write none.

NOTE: If the Athlete has several declarations to be recorded and there is not enough

room in the space provided on the Doping Control Form, continue on a Supplementary Report Form, and record the Supplementary Report Form Number on the Doping Control Form. This Supplementary Report Form must not include the Athlete’s name or initials and will not be signed by the Athlete.

NOTE: DCOs must not offer advice on substances/medications, question the purpose

of any medication or enter into any discussion on the status of a medication.

17.The DCO should ask the Athlete to read and complete the Consent for Research area of the Doping Control Form. This includes the Athlete signing this area of the form.

18.The DCO should complete theDoping Control Form. The DCO who processes the Athlete’s sample(s) is responsible for ensuring that the form is complete, accurate and legible. If any of the information on the Doping Control Form is not applicable, the DCO should draw a line through each non-applicable area, and check the N/A box, if available.

NOTE: The Doping Control Form should be filled out as completely as possible before

reviewing it with the Athlete. The only information that should be left blank during the review is the signature lines of the applicable persons present (e.g. DCO, Athlete Representative and Athlete).

NOTE: The DCO should confirm that the appropriate Athlete identification information

was provided. Acceptable forms of identification include a driver’s license, passport, sport federation identification/license card, doping control passport, etc.

19.Once the Doping Control Form is completed, the DCO should thoroughly review it with the Athlete and Athlete Representative, if present. If there are any mistakes on the Form, a new one should be re-written and the Form with the error should be voided and torn up while in the Athlete’s presence. This voided Form should be returned to the ADO along with the other sample collection documentation.

20.If the DCO, Athlete, Chaperone or Athlete Representative express an interest in making written comments specific to the Athlete’s sample or the testing session, they may do so

6.2 Urine Sample Processing Procedure

© WADA – DCO Manual - Version 3.0 – May 2011

on the Doping Control Form or, if additional space is required, on a Supplementary Report Form. The Supplementary Report Form number will be recorded on the Doping Control Form.

21.The DCO, Athlete Representative (if applicable), and Athlete will then print and sign their names on the Doping Control Form to verify the accuracy of the information.

NOTE: The DCO and any other applicable person besides the Athlete should sign first.

The Athlete should be the last person to sign the Doping Control Form. When the DCO signs the Form, he/she will also record the time of completion on the Doping Control Form.

22.The DCO should provide the appropriate copy(s) of the Doping Control Form and, if

applicable, the Supplementary Report Form to the Athlete at the conclusion of the Sample Collection Session.

NOTE: If a Supplementary Report Form was filled out, the DCO should record the

Form number on the Doping Control Form to link all necessary sample collection documentation to the Athlete's test.

NOTE: If an error on any of the Athlete's sample collection documentation is noticed

after the Athlete was released from the doping control station, the document should not be altered. In this case, the DCO should complete a Supplementary Report Form explaining the error and the Form number should be recorded on the Doping Control Officer Report Form.

6.2 Urine Sample Processing Procedure

© WADA – DCO Manual - Version 3.0 – May 2011

Summary Checklist

 The Athlete’s sample meets minimum volume requirements of 90 mL of urine  The Athlete has a minimum of three (3) sample collection kits to choose from

 The Athlete verifies that the A and B bottles and all other parts of the sample collection kit have the same sample code number

 The minimum amount of 30 mL of urine is poured into the B bottle  The minimum amount of 60 mL of urine is poured into the A bottle  The A and B bottles are securely sealed

 The details of the bottle number, volume and specific gravity are recorded on the Doping Control Form

 All applicable areas of the Doping Control Form are completed and a line is drawn through any non-applicable areas