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Virion-Independent Transfer of Replication-Competent Hepatitis C Virus RNA between Permissive Cells

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FIG 1 Virion-independent RNA transfer between Huh7 cells. A total of 1 � 105 SGR cells were cocultured with 1 � 105 Huh7-RVblastR-YFP cells for 2 daysin the absence of selection
FIG 2a Zeiss LSM 710 laser scanning confocal microscope. Both positive- and negative-strand HCV RNA are readily detected in cells selected for RNA transferstained by Hoechst33342 dye (blue; Life Technologies product no
FIG 3 Chromosomal DNA transfer leads to polyploidy, whereas RNA transfer does not. (A) Donor and recipient cells were cocultured as described forFig
FIG 4 Exosome release inhibitors GW4869 and spiroepoxide inhibit RNAby a two-tailed, paired Studentwas transfected into uninfected Huh7 cells using Mirus TransIT mRNA trans-fection reagent (Mirus Bio, Madison, WI) according to the manufacturer’sinstruction

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