Copyright © 2001, American Society for Microbiology. All Rights Reserved.
DNA Replication Efficiency Depends on Transcription
Factor-Binding Sites
WILLIAM J. TURNER†ANDMARY E. WOODWORTH*
Department of Microbiology, Miami University, Oxford, Ohio 45056
Received 29 November 2000/Accepted 27 March 2001
Naturally arising variants of simian virus 40 (SV40), generated by serial passage of the virus at high multiplicities of infection, provide important insight into the role of transcription factor-binding sites in enhancing DNA replication. Although the variants that arise from numerous recombination events are the result of selective pressure to replicate more efficiently than the other variants in the infection, there is no transcription pressure. Therefore, it is interesting that a minimum of two viral Sp1 transcription factor-binding sites are retained and that host AP-1 and NF-1 transcription factor-factor-binding sites are incorporated into the 100-bp regulatory region that maximizes DNA replication in these variants. We cotransfected COS-1 cells (that provide viral large T antigen for DNA replication) to examine the effect of transcription factor-binding sites on the replication of plasmid constructs that contain the SV40 origin of replication (ori). The level of relative replication efficiency (RRE) depends on the number and type of transcription factor-binding sites. Replication increases as the number of transcription factor-binding sites increases within the regulatory region of the variants; AP-1 sites are more effective than NF-1 transcription factor-binding sites. Competition between constructs in transfections magnifies the difference in their RREs. The results indicate that transcription factor-binding sites play an important role in enhancing DNA replication.
Simian virus 40 (SV40) DNA is replicated by host cell ma-chinery (reviewed in references 5 and 11) and one virally en-coded protein, large T antigen (T-ag) (6, 45, 50, 56). SV40 DNA replication is bidirectional (7, 17) from a 64-bp core origin of DNA replication (ori) (4, 14, 25, 45, 52). Thisori
sequence consists of a 15-bp early palindrome, a 27-bp palin-drome, and a 17-bp AT-rich region (8). Four pentanucleotides in the central 27-bp palindromic region oforiare binding sites for T-ag (9, 49, 57). Additional regulatory sequences within 180 bp on the late transcription side of ori include three 21-bp repeats (16) and a 72-bp transcriptional enhancer (2, 20, 23, 43). Each 21-bp repeat contains two hexameric GC boxes, which are binding sites for the transcription factor Sp1 (15) and weak binding sites for T-ag (57). The transcriptional enhancer contains a number of protein-binding sites; of these, a binding site for the transcription factor AP-1 is the closest toori(31, 34, 63).
The involvement of transcription factor-binding sites and the way in which they are arranged within regions that regulate DNA replication are interesting for several reasons. Some arrangements of AP-1 and Sp1 transcription factor-binding sites seem to be favored in evolutionary variants (37). Evolu-tionary variants arise when SV40 is serially passaged at high multiplicities of infection, whereby recombination leads to new viral species containing deleted and duplicated viral DNA and substituted host DNA. In several SV40 evolutionary variants (ev1101, ev1103, ev1104, and ev1108), the incorporated host sequences that regulate replication contain AP-1 and NF-1
transcription factor-binding sites on the late-transcription side of the SV40 Sp1 sites andori(26, 54, 62; M. Currier and M. E. Woodworth, unpublished data). The sequence context of an AP-1 transcription factor-binding site influences the effect of the AP-1 site on DNA replication efficiency (54; C. Hodson and M. E. Woodworth, unpublished data; H. Van Buskirk and M. E. Woodworth, unpublished data). Some of the past work involving the effects of Sp1 transcription factor-binding sites did not take the effects of an adjacent AP-1 transcription fac-tor-binding site into account (37, 52). The introduction of synthetic Sp1 transcription factor-binding sites to theori up-stream regulatory region in SV40 allowed measurement of their effects on replication efficiency (35).
Furthermore, transcription factor-binding sites are func-tional elements of a variety of viral and eukaryotic DNA rep-lication systems. Human papillomavirus type 18 contains a single binding site for the transcription factor Sp1 in its core origin of replication and two AP-1 sites in nearby auxiliary regions, as well as sites for several other transcription factors, both cellular and viral (10). Human papovavirus BK contains, in its enhancer region, two Sp1 sites, each adjacent to a binding site for NF-1 (13). Two sites downstream of the lytic origin of Epstein-Barr virus are protected from DNase I cleavage by Sp1 (22). The B3 regulatory element is essential forARS1activity in yeast and is bound by the transcription factor Abflp, which enhances origin utilization (41). The autonomously replicating monkey sequenceors8contains a binding site for the transcrip-tion factor Oct-1 (58). B48bs, a sequence that contains a hu-man origin of DNA replication, interacts with the transcription factor USF (59). The RPS14 replication origin of Chinese hamster ovary cells contains an Sp1 site and several AP-1 sites (55). Finally, the involvement of transcription factor-binding sites in increasing the efficiency of DNA replication would lend support to reports that transcription factors play a bifunctional * Corresponding author. Mailing address: Department of
Microbi-ology, Miami University, 32 Pearson Hall, Oxford, OH 45056. Phone: (513) 529-7249. Fax: (513) 529-2431. E-mail: woodwome@muohio .edu.
† Present address: Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY 10021.
5638
on November 9, 2019 by guest
http://jvi.asm.org/
role as enhancers of both transcription and replication (3, 11, 39, 44).
The present study investigates the effect of the spatial rela-tionships between binding sites for transcription factors AP-1, NF-1, and Sp1 on the replication efficiency of SV40oriand the number and combination of transcription factor-binding sites in SV40 regulatory regions and in selected host sequences. The results demonstrate that both host sequences and transcription factor-binding sites in the regulatory regions of SV40 evolu-tionary variants play a role in determining relative DNA rep-lication efficiency (RRE). The binding sites for the transcrip-tion factor AP-1 in evolutranscrip-tionarily selected regulatory regions cooperate with adjacent transcription factor-binding sites to enhance the replication efficiency ofori. The number and type of transcription factor-binding sites, and their distance from
ori, modulate their effect on the DNA replication efficiency of SV40ori-containing plasmids. Competition between constructs also has a significant effect on RRE. The data extend the findings of Sun and Hurley (53), Han and Hurley (28), and Wilderman et al. (61) that suggest a model in which interac-tions of transcription factors with DNA and replication pro-teins such as SV40 T-ag contortorito favor initiation of DNA replication.
MATERIALS AND METHODS
Plasmids.The plasmids pOri, pC133, and pC200 (38) contain an insertion of SV40 DNA sequence at theHindIII site of pKP45, a plasmid vector derived from pBR322 by the deletion of nucleotides 675 to 2364, which are poisonous for plasmid replication in mammalian cells (30, 40, 46). The insert in pOri is a 111-bp sequence including the 64-bp SV40 coreori. The 133-bp insert of pC133 contains ori, two GC boxes, and an AP-1 site. The pC200 insert consists of 200 bp, including ori, six GC boxes, and an AP-1 site. Plasmids pOri21, pCN163, pOri2x21, pOri2x21APp, pOri2x21APd, pOri3x21, and pOri3GC were con-structed by removing the sequences between theClaI andNcoI restriction en-donuclease sites of pC133 and replacing them with complementary oligonucle-otides withClaI andNcoI cohesive ends and containing various reiterations of the AP-1 site and the 21-bp repeat (containing the GC-hexamer-binding sites for Sp1). pC133CN75 contains a 75-bpClaI/NcoI insert that includes three Sp1-binding sites and 42 bp of host␣-satellite DNA. pC133H03 contains 71 bp of the host␣-satellite DNA present in evolutionary variantev1103 and was generated by synthesizing oligonucleotides representing␣-satellite DNA flanked byClaI cohesive ends and annealed to form a double-stranded 75-bp fragment; it was phosphorylated and ligated into theClaI site of pC133. The sequence of the insert was 5⬘-CGATTCATCTCACACAGTTACATCTTTCCCTTCAAGAAG CCTTTCACTATGGCTGTTCTTGTTGAATTGGCACAT-3⬘, with a CG dinu-cleotide overhang on the 5⬘ends of both strands. Clones in the desired orien-tation were identified by restriction endonuclease digestion withTfiI andEcoRV. To generate pC133⌬, pC133 was digested withEcoRV andBamHI, deleting a 190-bp fragment of plasmid DNA. Similarly, the 190-bpEcoRV/BamHI frag-ment of plasmid DNA was deleted in pOri⌬, pC200⌬, pOri2X21⌬, pOri3GC⌬, and pCN163⌬.
Cell culture and transfection.The COS-1 line of African green monkey kidney cells (21) was grown in Eagle’s minimal essential medium and 5% fetal bovine serum (MEM-5) at 37°C under 5% carbon dioxide and 90% humidity. When monolayers of COS-1 cells were 50% confluent, they were cotransfected with 50 ng each of test and control plasmids in the presence of 250g of DEAE-dextran/ml in 10⫻MEM with 50 mMN-2-hydroxyethylpiperazine-N⬘ -2-ethane-sulfonic acid (HEPES, pH 7.5) as previously described (38). After rocking of the cultures every 5 min for 30 min at 37°C, the cells were washed and supplemented with medium containing 100M chloroquine diphosphate for 3 h. The chloro-quine solution was removed, 5 ml of MEM-5 was added, and the cells were incubated at 37°C.
Analysis of RREs.Cells were lysed 48 h after transfection and Hirt (32) supernatants were prepared. After treatment with proteinase K, extraction with phenol-chloroform (1:1), and precipitation with cold isopropanol, the DNA was linearized withEcoRI and fractionated on a vertical 0.8% agarose gel (40 cm long, 1.5 mm thick) at 3 V/cm for 21 h. The region of interest was excised from
the agarose gel and treated for 10 min with 0.25 M HCl, 40 min with denatur-ation buffer (0.5 M NaOH, 1.5 M NaCl), and 40 min with neutralizdenatur-ation buffer (3 M NaCl, 1 M Tris-HCl, pH 5.0). The DNA was transferred by pressure blotter (Stratagene) for 30 min at 75 mm Hg onto a nitrocellulose membrane (BA85; 0.45-m pore size; Schleicher & Schuell, Inc.) The membrane was incubated with prehybridization buffer at 42°C for 4 h (5⫻SSC–5⫻Denhardt’s salt solution [1⫻SSC is 0.15 M NaCl plus 0.015 M sodium citrate], 50 mM NaPO4, pH 6.5, 167g of sheared salmon sperm DNA/ml, 50% deionized formamide, 0.3% sodium dodecyl sulfate), hybridized with [␣-32P]dCTP-labeled plasmid DNA (106cpm/50 to 100 ng) for 20 h, and visualized by autoradiography. The radio-active probe was prepared by random oligonucleotide priming and synthesis by the large (Klenow) fragment ofE. coliDNA polymerase I in the presence of [␣-32P]dCTP. The RREs of the SV40ori-containing plasmids were quantified by image analysis (NIH Image 1.52, National Institutes of Health [http://rsb.info .nih.gov/nih-image]).
RESULTS
Comparison of single and dual AP-1:Sp1:Sp1 motifs. One way to define sequences that are important for efficient DNA replication is to look for sequences common to genomes that replicate efficiently. Wild-type SV40 and variants of SV40 con-tain Sp1 transcription factor-binding sites in pairs, while AP-1 sites are found singly. For example, the SV40 evolutionary variant ev1104 contains invertedoris, one with a pair of Sp1 sites and an AP-1 site and the other with two pairs of Sp1 sites and two single AP-1 sites (Fig. 1a). The regulatory region of a passage 40 (p40) variant contains a pair of Sp1 sites and an AP-1 site, which were cloned into theHindIII site of pKP45 to form pC133 (38). The regulatory region of a passage 45 (p45) variant contains two copies of the earlier (p40) variant regu-latory region, which had presumably been duplicated during passage. This duplicated regulatory region was ligated between theClaI andNcoI sites of pC133 to produce pCN163 (Fig. 1a). Input mixtures containing equimolar amounts of pCN163 and pC133⌬ (test and control plasmid DNA, respectively) were used to transfect COS-1 cells. Aliquots of the input mixture and plasmid DNA purified 48 h after transfection were linear-ized by EcoRI digestion. By 48 h posttransfection, the input plasmid DNA has been degraded (37), and therefore, the ratio between the two bands of replicated pCN163 and pC133⌬
DNA represents the replication efficiency of one relative to the other. Division of this ratio by the ratio of bands produced by electrophoresis of the input mixture compensated for slight differences in the molarities of input DNA. pCN163 containing the later passage variant regulatory region replicated approx-imately nine times as efficiently as pC133 containing the earlier passage variant regulatory region (Fig. 2). pC133⌬lacks plas-mid sequences fromBamHI toEcoRV sites; this 190-bp dele-tion does not affect its RRE and allows electrophoretic sepa-ration from competing constructs. Figure 2 is a typical autoradiogram and representative of the experiments that are described below and summarized in Fig. 3.
Effect of host sequences on RRE.Evolutionary variants of SV40 have upstream auxiliary sequences that enhance DNA replication (37, 54, 62; Currier and Woodworth, unpublished). Many variants have incorporated host auxiliary sequences that presumably provide a replicative advantage. The genome of a passage 13 variant,ev1103, consists of nine 440-bp repeat units, each containing the SV40oriand three Sp1 transcription fac-tor-binding sites. Each repeat unit also contains 135 bp of low-copy-number monkey DNA and 143 bp of the 172-bp monkey ␣-satellite DNA monomer (36, 42). The ␣-satellite
on November 9, 2019 by guest
http://jvi.asm.org/
DNA sequence is highly repeated (48), but its function in the monkey genome is unclear.
The ␣-satellite sequence is interesting because it occupies the position of the wild-type upstream auxiliary sequences im-mediately adjacent to three Sp1 sites andori(Fig. 1b). The first 71 bp of the ␣-satellite region of ev1103 occupy the same relative position as the fourth, fifth, and sixth Sp1 sites in wild-type SV40. Becauseev1103 is a highly efficient replicator, it was important to determine whether these 71 bp were func-tionally analogous to the Sp1 transcription factor-binding sites in enhancing replication. A Signal Scan search for mammalian and viral transcription factor-binding sites (47) showed that the 71 bp of ␣-satellite DNA contained three binding sites for transcription factor NF-1 but lacked binding sites for AP-1.
␣-Satellite DNA in the context of pC133. The regulatory region of pC133 includes a pair of Sp1 transcription factor-binding sites and an AP-1 transcription factor-factor-binding site and replicates approximately three and a half times more efficiently than pOri (Fig. 3j). When 71 bp of the␣-satellite DNA (con-taining three NF-1 binding sites) fromev1103 were inserted on the late-transcription side of the regulatory region in pC133 to generate pC133H03 (Fig. 1b), the plasmid construct replicated
nearly five times more efficiently than pOri (Fig. 3k). Thus the
␣-satellite insert increased the RRE of pC133 by nearly 1.5-fold.
␣-Satellite DNA in the context of three Sp1 sites.Inev1103,
␣-satellite DNA is found on the late-transcription side of three binding sites for the transcription factor Sp1 (Fig. 1b). To test the effect of␣-satellite DNA in this, its original context, plas-mid constructs (pC133CN75 and pOri3GC) containing three Sp1 sites, with or without an additional 42 bp of ␣-satellite DNA, respectively, were compared (Fig. 3l). The ␣-satellite DNA containing two NF-1 binding sites increased RRE slightly (1.3-fold⫾0.2-fold).
A single AP-1 site in the context of one pair of Sp1 sites.To determine the effect of an AP-1 transcription factor-binding site on RRE, plasmid constructs that are identical except for the AP-1 transcription factor-binding site were required. To generate pOri21, complementary oligonucleotides were syn-thesized that contained a pair of Sp1 sites (one 21-bp repeat) and the spacing of the original AP-1 region, although four nucleotides were changed to inactivate the AP-1 site (1, 29). FIG. 1. Relationship of plasmid constructs to evolutionary variants.
The open arrow representsori. Solid bars represent host DNA incor-porated into evolutionary variantsev1103,ev1104, and ev1108, with pairs of diagonal lines to indicate that the entire sequence is too long to be shown. Squares with an “x” represent AP-1 transcription factor-binding sites; squares with a central dot represent Sp1 transcription factor-binding sites. (a) The sequence cloned into pCN163 is derived from the regulatory region ofev1104; the region cloned into pC133 is derived from ev1108. (b) The derivation of pC133H03 and pC133CN75 fromev1103 and pC133, respectively.
FIG. 2. RREs of portions of the regulatory regions of two evolu-tionary variants, ev1108 and ev1104. The top pair of bands in the autoradiogram is the input mixture; the bottom pair of bands repre-sents DNA harvested 48 h posttransfection. The bottom construct (control plasmid) in each group of bands had a 190-bp deletion (⌬) in plasmid DNA and served as the competitor construct in each compar-ison. Three separate experiments are shown in lanes 1 to 3. At the bottom of the figure, the pair of plasmid constructs used in the co-transfection is depicted schematically. Open arrows represent ori. Squares with a central dot represent Sp1 transcription factor-binding sites; squares with an “x” represent AP-1 transcription factor-binding sites. Results are presented in terms of the relative DNA replication efficiency (RRE) of the test construct (pCN163) with respect to the competing, control construct (pC133⌬), ⫾ the standard deviation (SD). The number of experiments (n) is indicated in parentheses.
on November 9, 2019 by guest
http://jvi.asm.org/
The addition of one pair of Sp1 sites (pOri21) to pOri en-hanced replication 2.2⫾0.5 times (Fig. 3i). However, in four separate experiments, pOri21 replicated only 0.15⫾0.05 times as well as pC133⌬(Fig. 3b). The data indicate that the AP-1 site increases RRE 6.7-fold in the context of a pair of Sp1 transcription factor-binding sites.
A single AP-1 site in the context of two pairs of Sp1 sites. The regulatory region of pCN163 containsoriplus two pairs of Sp1 sites alternating with two AP-1 transcription factor-bind-ing sites. To determine the effect of a sfactor-bind-ingle AP-1 transcription factor-binding site in the context of two pairs of Sp1 sites, variations were constructed by mutation of either one or both of the AP-1 sites in this regulatory region. One contained an intact AP-1 transcription factor-binding site proximal toori
(pOri2x21APp), the second contained an intact AP-1 tran-scription factor-binding site distal toori(pOri2x21APd), and in the third (pOri2x21), both AP-1 transcription factor-binding
sites were inactivated. The AP-1 sites were inactivated while maintaining the spacing of the original region by changing four nucleotides of the AP-1 site (1, 29). pOri2x21⌬was used to provide a baseline level of replication. In the context of two pairs of Sp1 sites, an AP-1 site proximal toori(pOi2x21APp) increased RRE 4.5-fold⫾0.2-fold; an AP-1 site distal toori
(pOri2x21APd) increased RRE 4.7-fold⫾0.6-fold (Fig. 3c and d). The data indicate that, regardless of its position, an AP-1 site increases the RRE of a construct containing two pairs of Sp1 sites.
Location of an AP-1 site relative to two pairs of Sp1 sites. The difference in replication between pOri2x21⌬, the baseline control, and either pOri2x21 APp or pOri2x21APd was greater than fourfold, but the variance was too high to judge whether the proximal or distal AP-1 transcription factor-binding site had a greater effect on the RRE of the construct. To reduce experimental variation, pCN163, which contains a regulatory FIG. 3. Summary of data generated by cotransfections of test and control plasmid DNAs. Each construct is depicted schematically. Open arrows representori; squares with a central dot represent Sp1 transcription factor-binding sites; squares with an “x” represent AP-1 transcription factor-binding sites; solid bars represent host DNA incorporated into evolutionary variants. Results are presented in terms of the relative DNA replication efficiency (RRE)⫾the standard deviation (SD) of the test construct with respect to the competing, control construct (normalized to 1.00). The number of experiments (n) is indicated in parentheses.
on November 9, 2019 by guest
http://jvi.asm.org/
region with two pairs of Sp1 transcription factor-binding sites and two intact AP-1 transcription factor-binding sites, was used as a basis for comparison. This construct, being closer in rep-lication efficiency to pOri2x21APp and pOri2x21APd, allowed the determination of their RREs with the benefit of much less variance. pOri2x21APp, with an intact proximal AP-1 site, rep-licated 0.36 ⫾ 0.05 times as efficiently as pCN163⌬, while pOri2x21APd, with a distal AP-1 site, replicated 0.54 ⫾0.02 times as efficiently as pCN163⌬(Fig. 3e and f). That is, the distal AP-1 transcription factor-binding site increased replica-tion efficiency more than the proximal AP-1 transcripreplica-tion fac-tor-binding site.
Addition of AP-1 sites to two pairs of Sp1 sites.The addition of transcription factor-binding sites could affect relative DNA replication efficiency in a number of ways. Each added site could produce an additive increase in replication efficiency, or one of the added sites could be responsible for all of the observed increase in RRE. As shown in Fig. 3c and d, the addition of one AP-1 site to a construct containing two pairs of Sp1 sites increased replication efficiency relative to that of only two pairs of Sp1 sites. Furthermore, pCN163, with two pairs of Sp1 sites and two AP-1 sites, replicates more efficiently than either pOri2x21APp or pOri2x21APd, each of which has two pairs of Sp1 sites but only one AP-1 site (Fig. 3e and f).
A single AP-1 site in the context of three pairs of Sp1 sites. pC200 (38), containing three pairs of Sp1 sites (one pair in each of its three 21-bp repeats) and an AP-1 site, produces the wild-type maximum replication efficiency from ori (38, 52). When the AP-1 site on the late-transcription side of the three Sp1 pairs is deleted from its natural context in the 72-bp tran-scriptional enhancer, replication efficiency is decreased (27). It was of interest to determine whether the AP-1 site found on the late-transcription side of the three pairs of Sp1 transcrip-tion factor-binding sites in wild-type SV40 increases replicatranscrip-tion in the absence of the remainder of the 72-bp transcriptional enhancer. A pair of complementary oligonucleotides contain-ing the three pairs of Sp1 sites was synthesized without the terminal AP-1 site; the RRE of the new construct, pOri3x21, when compared with that of pC200⌬, was 0.9⫾0.3 (Fig. 3g). Thus, the presence or absence of the AP-1 site in the context of three pairs of Sp1 sites had little or no effect.
Substitution of two AP-1 sites for two of six Sp1 sites. Sub-stitution of one transcription factor-binding site for another addressed relative effects on replication efficiency. pOri3x21, whose regulatory region contains three pairs of Sp1, and pCN163, in which the third and sixth Sp1 sites proximal toori
were replaced with AP-1 sites, were independently compared with pC200, which contains the wild-type SV40 regulatory re-gion with six Sp1 sites and one AP-1 site. The RRE of pCN163 was 0.9, as was the RRE of pOri3x21 (Fig. 3g and h), or equivalent to that produced by the wild-type regulatory region of pC200. Therefore, the substitution of two AP-1 sites for two of the six Sp1 sites resulted in no significant change in RRE.
DISCUSSION
To gain further insight into the role of transcription factor-binding sites in replication, we analyzed the RREs of various combinations of transcription factor-binding sites.
The regulatory regions of evolutionary variants contribute to the variants’ replicative advantage over wild type.The num-ber, type, and arrangement of transcription factor-binding sites in the regulatory region of variants help determine their rep-lication efficiency. A dramatic ninefold increase in reprep-lication occurred when the transcription factor-binding site combina-tion of one AP-1 site and two Sp1 sites was duplicated (as occurs during the naturally arising evolution of variants from serial passage 40 to 45). The ninefold enhancement is signifi-cantly greater than the 1.4-fold increase seen when a 71-bp monkey␣-satellite DNA sequence (found in an earlier, 13th passage variant,ev1103) containing three NF-1 sites is added to the transcription factor-binding site combination of one AP-1 site and two Sp1 sites. Thus, two regulatory regions, each containing six transcription factor-binding sites, are not neces-sarily equivalent in their ability to enhance replication; i.e., four Sp1 plus two AP-1 sites (pCN163) are significantly better enhancers of replication than are two Sp1 plus one AP-1 plus three NF-1 sites (pC133H03). Presumably, the AP-1 and Sp1 sites play a more significant role in replication than do the NF-1 sites. The 40th and 45th passage variants (ev1108 and
ev1104) incorporated host sequences consisting of both AP-1 and NF-1 transcription factor-binding sites while the 13th pas-sage variant (ev1103) incorporated only NF-1 transcription factor-binding sites. These results are consistent with the as-sumption that the pressure of serial passage of virus through cells at high multiplicity of infection selects variants of in-creased replication efficiency and suggests that the number, type, and arrangement of transcription factor-binding sites are important.
The portion of the regulatory region ofev1104 (passage 45) containing two copies of the transcription factor-binding site combination of one pair of Sp1 sites and an AP-1 site produces the same replication efficiency as three pairs of Sp1 transcrip-tion factor-binding sites or three pairs of Sp1 sites and an AP-1 transcription factor-binding site (the wild-type regulatory re-gion). The entire variant regulatory region ofev1104 includes two copies oforiin an inverted repeat and a central, host DNA sequence containing one NF-1 transcription factor-binding site and an additional AP-1 transcription factor-binding site (26). Deletion of one or both AP-1 sites from the regulatory region of evolutionary variantev1104 resulted in decreased replica-tion efficiency (54) and helps explain why AP-1 sites have been conserved in evolutionary variants of SV40.
The AP-1 site has a range of context-dependent effects.An AP-1 site has a greater effect on RRE where there are fewer pairs of Sp1 transcription factor-binding sites present. It should be noted that the configuration of transcription factor-binding sites found in the naturally arising late-passage evolutionary variantsev1104 andev1108 consists of a transcription factor-binding site combination of a host AP-1 site and a pair of viral Sp1 sites, a configuration that maximizes the impact of the AP-1 site on replication efficiency and perhaps favors the pro-duction of this particular configuration of binding sites in serial passage.
Haas et al. (27) made two separate plasmid constructs, each with three contiguous substitution mutations in the AP-1 site of the SV40 72-bp transcriptional enhancer. The enhancer also contains binding sites for several other transcription factors. They used these constructs in the same type of replication
on November 9, 2019 by guest
http://jvi.asm.org/
assay used for the present study (transient transfection of COS-1 cells) to show that the AP-1 site increases replication efficiency in the context of the 72-bp transcriptional enhancer region on the late side of wild-type SV40ori. Disruption of the AP-1 site decreased RRE by 35 to 38 percent (27). The present study demonstrates that the AP-1 site in wild-type SV40, when isolated from the other transcription factor-binding sites of the 72-bp enhancer, had little effect on replication efficiency (pC200 compared with pOri3x21). This suggests that some additional interaction between bound transcription factors is necessary for the AP-1 site to increase replication efficiency from the late-transcription side of six Sp1 sites.
The location, number, and context of AP-1 sites appear to be factors in replication efficiency. In the context of two pairs of Sp1 sites, two AP-1 transcription factor-binding sites enhance replication more than one AP-1 transcription factor-binding site. Furthermore, an AP-1 site located within a region con-taining two pairs of Sp1 sites increased replication whether proximal or distal toori. However, an AP-1 site distal toori
increased RRE more than an AP-1 site proximal toori. These results are consistent with the inhibitory activity of a sole AP-1 site proximal toori, in the absence of any additional transcrip-tion factor-binding sites (P. Szymanski and M. E. Woodworth, unpublished data), and suggest that the effect of an AP-1 site on replication might correlate with its distance fromori. The enhanson model of transcriptional activation in which two ad-jacent transcription factor-binding sites form one functional enhancing unit may apply to DNA replication as well as tran-scription (19). The interaction of adjacent bound trantran-scription factors could play a critical role in the activation of DNA replication.
Because both Sp1 and AP-1 sites can function as activators of DNA replication, their relative enhancing activities were investigated. An AP-1 site was substituted for two of the six Sp1 sites that constitute the wild-type SV40 regulatory region (pCN163 compared with pC200). In this specific context, the enhancement of replication by a single AP-1 site was equiva-lent to that of a pair of Sp1 sites. Sp1 sites are found in pairs in both wild-type SV40 and variants, and a pair seems to functionally replace an AP-1 site. When compared with a plas-mid construct (pOri) containing SV40oriwith no additional viral regulatory sequences, the addition of two viral Sp1 tran-scription factor-binding sites (pOri21) enhances replication and the addition of an AP-1 site to the two viral Sp1 transcrip-tion factor-binding sites (pC133) further enhances replicatranscrip-tion. These data emphasize the importance of both the Sp1 and AP-1 sites.
Competition between constructs exerts a significant effect on RRE.The competitive assay of replication efficiency used in this study provides competitive conditions similar to those that generate naturally arising evolutionary variants in serial cell culture infection. Our studies show that a construct that con-tainsori, two Sp1 sites, and an AP-1 site (pC133) competes much better against pOri21 (oriand two Sp1 sites) than against pOri (6.7-fold difference in replication efficiency compared to a 3.5-fold difference, respectively). Because pOri contains the SV40 ori without any late-transcription-side auxiliary se-quences, it would seem to provide less competition for tran-scription factors. If there is heightened competition between constructs for scarce protein resources, the better competitor
sequestering more assures its replication. Sequestration of pro-tein resources would increase the chance that some of the proteins would become limiting. It has been shown that T-ag is the limiting factor in cotransfections (37). Transcription factor-binding sites might also affect replication efficiency by height-ening competition for T-ag. Competition with a construct that lacks transcription factor-binding sites would result in a longer period of time before T-ag would become limiting.
Studies of the dynamics of SV40 DNA replication in vitro have called into question the role of transcription factors. Bul-lock (5) found no significant difference in replication of SV40
oriwith or without auxiliary transcription factor-binding sites. The results of the present study were generated in vivo and extended over 48 h, while the in vitro study was concluded in 20 min. The coactivator for Sp1 is labile at the temperatures used for in vitro replication (18). In addition, their results were generated without competitor constructs. The results argue that transcription factor-binding affinities must be taken into account where competition for resources is a factor in DNA replication. Moreover, the differences between the in vivo and in vitro data suggest that transcription factor-binding sites play an important role in a chromatin environment but not when nucleosomes are absent.
The competitive cotransfection of mammalian cells offers a quantifiable model of phenomena that occur in nature. In infections with DNA viruses, the viral origin of replication must compete with the host origins, which have the advantage of superior numbers early in the infection. A more efficient virus is likely to be a better competitor for initiation factors. Cotransfection experiments might correlate with competition that occurs between host replication origins. Origins in mam-malian chromosomes have not been associated with any dis-tinctive sequence, and origins with different sequences may have different affinities for requisite initiation factors. With the exception of T-ag, the initiation factors for the SV40oriare drawn from the pool of cellular initiation factors. Neoplastic cells escape the conventional controls on DNA synthesis and cell proliferation. Origins of replication in the chromosomes of these cells might have improved ability to gather initiation factors. Competitive replication experiments can lay the groundwork for the study of these phenomena.
A model describing how interactions with transcription fac-tors affect replication efficiency.The AP-1 site in the enhancer of polyomavirus promotes T-ag-mediated DNA unwinding (24). Transcription factors ZBP-89 and Sp1 stimulate DNA replication of Epstein-Barr virus by tethering viral replication proteins toori(3). The proline-rich activation domain of NF-1 interacts with histone H3 to activate SV40 DNA replication (44). DNA replication is also enhanced inSaccharomyces
cer-evisiaeby the activation domains of Sp1 and CTF1 (39). In
SV40, the binding of Sp1 to three of the weak binding sites for T-ag might increase the efficiency of assembly of the T-ag double hexamer over its strong site II binding sites in the 27-bp palindrome. The T-ag hexamers could separate to begin rep-lication of the DNA or they might remain together as a sta-tionary pump, pulling the DNA through as the polymerase replicates it (reviewed in reference 60). To advance the repli-cative process, any bound Sp1 must be displaced. A bound AP-1 transcription factor nearby could increase the efficiency of replication by affecting the configuration of the Sp1 sites so
on November 9, 2019 by guest
http://jvi.asm.org/
that their affinity for Sp1 is reduced. Alternatively, bound tran-scription factors could increase the local concentration of T-ag by interacting with it directly, perhaps coordinating its arrival at the 27-bp palindromic sequence.
In general, replication efficiency increases with the number of transcription factors present. This is true of the host se-quences integrated in evolutionary variants, which at higher passage numbers contain more transcription factor-binding sites. However, the system can become saturated with tran-scription factor-binding sites, as seen by the decreased en-hancement after adding an AP-1 site to greater numbers of Sp1 sites. To explain how an AP-1 site distal toori can enhance replication more than a site proximal toori, it is necessary to consider the effect of the intervening transcription factors bound to the regulatory region. Sp1 changes the conformation of DNA by inducing a bend in the DNA to which it binds (53). Multiple Sp1-binding sites are capable of producing a loop that could bring a distal AP-1 site near enough to interact with T-ag bound atori. If the Jun subunit of AP-1 interacts with SV40 T-ag as it does with polyoma large T-ag (24, 33), AP-1 could coordinate arrival of T-ag atorito facilitate formation of the T-ag hexamers. AP-1 also may interact with the T-ag hexamers, stabilizing the bent conformation imposed upon the DNA by Sp1, inducing distortion oforiDNA to facilitate initiation of DNA replication. Smelkova and Borowiec (51) demonstrated that the binding of T-ag is strongly dependent on the length of the single-stranded DNA of the replication bubble. An AP-1 transcription factor bound to a site proximal tooriwould be sterically hindered by Sp1 from interaction with T-ag bound at
oribut could enhance replication by further bending the Sp1-induced loop. A construct containing two AP-1 sites, proximal and distal toori, replicates better still, probably taking advan-tage of bending by the proximal site and any T-ag interactions of the distal site. That a single AP-1 site, in the absence of other transcription factor-binding sites, decreased replication efficiency fromori(Szymanski and Woodworth, unpublished) may be the result of the hypothetical destabilization of T-ag binding at site I posited in the model above. The elimination of all Sp1 sites would also remove weak T-ag binding at site III. It is possible that some minimum number of transcription factor-binding sites is required to induce bending, thus making it important that the host DNA that substitutes for three to four Sp1 sites inev1103, ev1104, and ev1108 provide a long enough stretch of transcription factor-binding sites to induce bending of the DNA. This distortion of the DNA at ori is probably required to facilitate the initiation of replication. Furthermore, the binding of transcription factors may alter nucleosome phasing and, thereby, enhance replication by fa-cilitating both bending of the DNA and binding of the repli-cation complex. Clearly, nuclear structure plays an essential role in DNA replication in vivo (12). Transcription factors may change the site of initiation of DNA replication and, thereby, change replication efficiency. It is of interest to note that at least one pair of viral Sp1 sites has been retained in all evolu-tionary variants studied to date. This suggests that not only is the combination of two Sp1 sites andorithe minimum viral sequence needed for replication, but the spacing between the Sp1 sites andorimust remain intact. Based on what we now know from the characterization of the regulatory regions that have evolved to give variants a replicative advantage, the term
“transcription factors” incompletely describes the functions of these important proteins since they may have been captured from the host to function primarily as part of the replication apparatus rather than as enhancers of transcription.
ACKNOWLEDGMENTS
We thank Bugen Hu for helpful discussions and P. J. Wilderman for helping format the figures.
This research was supported in part by Sigma Xi, The Scientific Research Triangle (W.J.T.), and Miami University.
REFERENCES
1.Angel, P., K. Hattori, T. Smeal, and M. Karin.1988. Thejunproto-oncogene is positively autoregulated by its product, Jun/AP-1. Cell55:875–885. 2.Banerji, J., S. Rusconi, and W. Schaffner.1981. Expression of a-globin
gene is enhanced by remote SV40 DNA sequences. Cell27:299–308. 3.Baumann, M., R. Feederle, E. Kremmer, and W. Hammerschmidt.1999.
Cellular transcription factors recruit viral replication proteins to activate the Epstein-Barr virus origin of lytic DNA replication,oriLyt. EMBO J.18:6095– 6105.
4.Bergsma, D. J., D. M. Olive, S. W. Hartzell, and K. N. Subramanian.1982. Territorial limits and functional anatomy of the simian virus 40 replication origin. Proc. Natl. Acad. Sci. USA79:381–385.
5.Bullock, P. A.1997. The initiation of SV40 DNA replicationin vitro. Crit. Rev. Biochem. Mol. Biol.32:503–568.
6.Chou, J. Y., J. Avila, and R. G. Martin.1974. Viral DNA synthesis in cells infected by temperature-sensitive mutants of simian virus 40. J. Virol.14:
116–124.
7.Danna, K. J., and D. Nathans.1972. Bidirectional replication of simian virus DNA. Proc. Natl. Acad. Sci. USA69:3097–3100.
8.Deb, S., A. L. DeLucia, C.-P. Baur, A. Koff, and P. Tegtmeyer.1986. Domain structure of the simian virus 40 core origin of replication. Mol. Cell. Biol.
6:1663–1670.
9.DeLucia, A. L., B. A. Lewton, R. Tjian, and P. Tegtmeyer.1983. Topography of simian virus 40 protein-DNA complexes: arrangement of protein sites at the origin of replication. J. Virol.46:143–150.
10. Demeret, C., M. Le Moal, M. Yaniv, and F. Thierry.1995. Control of HPV 18 DNA replication by cellular and viral transcription factors. Nucleic Acids Res.23:4777–4784.
11. DePamphilis, M. L. (ed.).1999. Concepts in eukaryotic DNA replication. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. 12. DePamphilis, M. L.2000. Review: nuclear structure and DNA replication. J.
Struct. Biol.129:186–197.
13. Deyerle, K. L., F. G. Sajjadi, and S. Subramani.1989. Analysis of origin of DNA replication of human papovavirus BK. J. Virol.63:356–365. 14. DiMaio, D., and D. Nathans.1980. Cold-sensitive regulatory mutants of
simian virus 40. J. Mol. Biol.140:129–142.
15. Dynan, W. S., and R. Tjian.1985. Control of eukaryotic messenger RNA synthesis by sequence-specific DNA-binding proteins. Nature316:774–778. 16. Everett, R. D., D. Baty, and P. Chambon.1983. The repeated G⫹C-rich motifs upstream from the TATA box are important elements of the SV40 early promoter. Nucleic Acids Res.11:1475–1489.
17. Fareed, G. C., C. F. Garon, and N. P. Salzman.1972. Origin and direction of simian virus 40 DNA replication. J. Virol.10:484–491.
18. Farnham, P. J., and M. M. Cornwell.1991. Sp1 activation of RNA polymer-ase II transcription complexes involves a heat-labile DNA-binding compo-nent. Gene Expr.1:137–148.
19. Fromental, C., M. Kanno, H. Nomiyama, and P. Chambon.1988. Cooper-ativity and hierarchical levels of functional organization in the SV40 en-hancer. Cell54:943–953.
20. Fromm, M., and P. Berg.1983. SV40 early- and late-region promoter func-tions are enhanced by the 72-base-pair repeat inserted at distant locafunc-tions and inverted orientations. Mol. Cell. Biol.3:991–999.
21. Gluzman, Y.1981. SV40 transformed simian cells support the replication of early SV40 mutants. Cell23:175–182.
22. Gruffat, H., O. Renner, D. Pich, and W. Hammerschmidt.1995. Cellular proteins bind to the downstream component of the lytic origin of DNA replication of Epstein-Barr virus. J. Virol.69:1878–1886.
23. Gruss, P., R. Dhar, and G. Khoury.1981. Simian virus 40 tandem repeated sequences as an element of the early promoter. Proc. Natl. Acad. Sci. USA
78:943–947.
24. Guo, W., W. J. Tang, X. Bu, V. Bermudez, M. Martin, and W. R. Folk.1996. AP1 enhances polyomavirus DNA replication by promoting T-antigen-me-diated unwinding of DNA. J. Virol.70:4914–4918.
25. Gutai, M. W., and D. Nathans.1978. Evolutionary variants of simian virus 40: nucleotide sequence of a conserved SV40 DNA segment containing the origin of viral DNA replication as an inverted repetition. J. Mol. Biol.
126:259–274.
on November 9, 2019 by guest
http://jvi.asm.org/
26.Gutai, M. W., and D. Nathans.1978. Evolutionary variants of simian virus 40: cellular DNA sequences and sequences at recombinant joints of substi-tuted variants. J. Mol. Biol.126:275–288.
27. Haas, M. W., P. Ramanujam, S. C. Chandrasekharappa, and K. N. Subra-manian.1991. Sequence requirements for activation of replication by the SV40 transcriptional promoter or enhancer elements. Virology180:41–48. 28. Han, F. X., and L. H. Hurley.1996. A model for the T-antigen-induced
structural alteration of the SV40 replication origin based upon experiments with specific probes for bent, straight, and unwound DNA. Biochemistry
35:7995–8001.
29. Han, T. H., W. W. Lamph, and R. Prywes.1992. Mapping of epidermal growth factor-, serum-, and phorbol ester-responsive sequence elements in the c-junpromoter. Mol. Cell. Biol.12:4472–4477.
30. Hanahan, D., D. Lane, L. Lipscich, M. Wigler, and M. Botchan.1980. Characteristics of an SV40-plasmid recombinant and its movement into and out of the genome of a murine cell. Cell21:127–139.
31. Herr, W., and J. Clarke.1986. The SV40 enhancer is composed of multiple functional elements that can compensate for one another. Cell45:461–470. 32. Hirt, B.1967. Selective extraction of polyoma DNA from infected mouse cell
culture. J. Mol. Biol.26:365–369.
33. Ito, K., M. Asano, P. Hughes, H. Kohzaki, C. Masutani, F. Hanaoka, T. Kerppola, T. Curran, Y. Murakami, and Y. Ito.1996. c-Jun stimulates origin-dependent DNA unwinding by polyomavirus large T-antigen. EMBO J.15:5636–5646.
34. Jones, N. C., P. W. J. Rigby, and E. B. Ziff.1988.Trans-acting protein factors and the regulation of eukaryotic transcription: lessons from studies on DNA tumor viruses. Genes Dev.2:267–281.
35. Lednicky, J., and W. R. Folk.1992. Two synthetic Sp1-binding sites func-tionally substitute for the 21-base-pair repeat region to activate simian virus 40 growth in CV-1 cells. J. Virol.11:6379–6390.
36. Lee, T. N. H., W. W. Brockman, and D. Nathans.1975. Evolutionary variants of simian virus 40: cloned substituted variants containing multiple initiation sites for DNA replication. Virology66:53–69.
37. Lee-Chen, G., and M. Woodworth-Gutai.1986. Evolutionarily selected ori-gins: functional aspects and structural organization. Mol. Cell. Biol.6:3077– 3085.
38. Lee-Chen, G., and M. Woodworth-Gutai.1986. Simian virus 40 DNA repli-cation: functional organization of regulatory elements. Mol. Cell. Biol.
6:3086–3093.
39. Li, R.1999. Stimulation of DNA replication inSaccharomyces cerevisiaeby a glutamine- and proline-rich transcription activation domain. J. Biol. Chem.
274:30310–30314.
40. Lusky, M., and M. Botchan.1981. Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences. Nature293:79–81.
41. Marahrens, Y., and B. Stillman.1992. A yeast chromosomal origin of DNA replication defined by multiple functional elements. Science255:817–823. 42. McCutchan, T., M. Singer, and M. Rosenberg.1979. Structure of simian
virus 40 recombinants that contain both host and viral DNA sequences. II. The structure of variant 1103 and its comparison to variant CVP8/1/P2 (EcoRI res). J. Biol. Chem.254:3592–3597.
43. Moreau, P., R. Hen, B. Wasylyk, R. Everett, M. P. Gaub, and P. Chambon.
1981. The SV40 72 base pair repeat has a striking effect on gene expression both in SV40 and other chimeric recombinants. Nucleic Acids Res.9:6047– 6068.
44. Muller, K., and N. Mermod.2000. The histone-interacting domain of nuclear factor I activates simian virus 40 DNA replicationin vivo. J. Biol. Chem.
275:1645–1650.
45. Myers, R. M., and R. Tjian.1980. Construction and analysis of SV40 origins defective in tumor antigen binding and DNA replication. Proc. Natl. Acad. Sci. USA77:6491–6495.
46. Peden, K. W. C., J. M. Pipas, S. Pearson-White, and D. Nathans.1980. Isolation of mutants of an animal virus in bacteria. Science209:1392–1396. 47. Prestridge, D. S.1996. SIGNAL SCAN 4.0: additional databases and
se-quence formats. Comp. Appl. Biosci.12:157–160.
48. Rosenberg, H., M. Singer, and M. Rosenberg.1978. Highly reiterated se-quences of simians. Science200:394–402.
49. Shalloway, D., T. Kleinberger, and D. M. Livingston.1980. Mapping of SV40 DNA replication binding sites for the SV40 T antigen by protection against exonuclease III digestion. Cell20:411–422.
50. Shortle, D. R., R. F. Margolskee, and D. Nathans.1979. Mutational analysis of the simian virus 40 replicon: pseudorevertants of mutants with a defective replication origin. Proc. Natl. Acad. Sci. USA76:6128–6131.
51. Smelkova, N. V., and J. A. Borowiec.1997. Dimerization of simian virus 40 T-antigen hexamers activates T-antigen DNA helicase activity. J. Virol.
7:8766–8773.
52. Subramanian, K. N., and T. Shenk.1978. Definition of the boundaries of the origin of DNA replication in SV40. Nucleic Acids Res.5:3635–3642. 53. Sun, D., and L. H. Hurley.1994. Cooperative bending of the 21-base-pair
repeats of the SV40 viral early promoter by human Sp1. Biochemistry33:
9578–9587.
54. Szymanski, P., and M. Woodworth.1989. A 69-base-pair monkey DNA sequence enhances simian virus 40 replication and transcription through multiple motifs. J. Virol.64:1360–1365.
55. Tasheva, E. S., and D. J. Roufa.1994. A mammalian origin of bidirectional DNA replication within the Chinese hamster RPS14 locus. Mol. Cell. Biol.
14:5628–5635.
56. Tegtmeyer, P.1972. Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon. J. Virol.10:591–598.
57. Tjian, R.1978. The binding site on SV40 DNA for a T antigen-related protein. Cell13:165–179.
58. Todd, A., S. Landry, C. E. Pearson, V. Khoury, and M. Zannis-Hadjopoulos.
1995. Deletion analysis of minimal sequence requirements for autonomous replication ofors8, a monkey early-replicating DNA sequence. J. Cell. Bio-chem.57:280–289.
59. Toth, E. S., L. Marusic, A. Ochem, A. Patthy, S. Pongor, M. Giacca, and A. Falaschi.1993. Interactions of USF and Ku antigen with a human DNA region containing a replication origin. Nucleic Acids Res.21:3257–3263. 60. West, S. C.1996. DNA helicases: new breeds of translocating motors and
molecular pumps. Cell86:177–180.
61. Wilderman, P. J., B. Hu, and M. E. Woodworth.1999. Conformational changes in simian virus 40 rearranged regulatory regions: effects of the 21-base-pair promoters and their location. J. Virol.73:10254–10263. 62. Woodworth-Gutai, M., A. Celeste, L. Sheflin, and M. Sclair.1983. Naturally
arising recombinants that are missing portions of the simian virus 40 regu-latory region. Mol. Cell. Biol.3:1930–1936.
63. Zenke, M., T. Grundstrom, H. Matthes, M. Wintzerith, C. Schatz, A. Wilde-man, and P. Chambon.1986. Multiple sequence motifs are involved in SV40 enhancer function. EMBO J.5:387–397.
