Simian virus 40 agnoprotein facilitates perinuclear-nuclear localization of VP1, the major capsid protein.

RLP secretion was observed only in cells ex- pressing CapE2SP and E2E1, despite the fact that relatively high levels of capsid proteins were detected in all cell lysates (Fig..

As expected, IP of the extract obtained from VT7/VP1- infected cells with the anti-VP1 produced a single band of 95 kDa, corresponding to VP1 (Fig. 9A, lane 8), that comigrated with

Identification of nuclear localization signal and nuclear export signal of VP1 from the chicken anemia virus and effects on VP2 shuttling in cells RESEARCH Open Access Identification

with mutant pm975 contained the 289R protein localized in the nuclear matrix (Fig. 6, lane d), while cells infected with mutant d11500 did not contain any detectable Ela protein in

These results indicate the presence of a protein in the plasma membrane of cells transformed or infected by simian virus 40 that is immunolog- ically indistinguishable from

immunoprecipitated proteins; (b) cells infected with SV40 SV-S, immunoprecipitated proteins; (c) cells infected with dl-1001 and B4, immunoprecipitated proteins; (d) uninfected

A similar major band comigrating with polyoma VP1 was synthe- sized in response to infected-cell mRNA in ex- tracts from Krebs II mouse ascites cells, L cells,.. and

Ad2+ND1 induces the synthesis of one known SV40 antigen, the SV40 U-antigen, which can be detected in the perinuclear region of infected cells (16).