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Antibiotic-free segregational plasmid stabilization in Escherichia coli owing to the knockout of triosephosphate isomerase (tpiA)

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Figure

Figure 1 Growth testing of the wild type MG1655 and Keiodeletion mutant strains in shake-flasks on a glycerol-basedLB medium.
Figure 4 Batch cultivation of E. coli strain JW3890-2-pFC1. TheKeio ΔtpiA knockout strain JW3890-2 complemented with theconstruct pFC1 was grown in SGA medium in a 2 L in-house fermenterwith 1 L working volume at 30°C without the use of antibiotics.
Figure 5 Batch cultivation ofThe Keioconstruct pFC4 was grown in SGA medium in a 2 L in-house fermenter E
Table 1 Primers for cloning an artificial terminatorbehind the tpiA gene on pJET-tpiA
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