THEPROBE 21. contents Stratech Focus. E: T: +44 (0) W: F: +44 (0)

page 00

contents

Stratech Focus

30th_{Anniversary Special Offers 02}

Stratech Super Sudoku Challenge  15

Our Products Now on Your E-Marketplace! 06

New Suppliers

Creative Diagnostics: Creative Research Products 03

GBI Labs: Enhanced Immunohistochemistry 03

LD BioPharma: Recombinant Human TF & Matrix Proteins 04

ApexBio: Bioanalytic Reagents - Apoptosis & Epigenetics 04

Affymetrix USB®_{: Molecular Biology Reagents & Biochemicals 05}

Phalanx Biotech: OneArray®_{DNA Microarrays 05}

Signosis Inc.: Innovative Plate Assay Solutions 06

Product Focus

Active Kinases & Phosphatases 07

RTDTM_Fluoro-Jade®_{C staining kit 08}

Find Your Protein’s Solubility Sweet Spot: OptiSol 09

PrecisionXTM_{Cas9 SmartNuclease}TM_{System from SBI 10}

MetaboLyteTM_{Production Platform 11}

Immunohistochemistry using Same Antibody & Tisssue Species 12

Cell Proliferation Assays 13

Human Cytokine ELISA Plate Arrays 14

THE

PROBE

News from Stratech Scientific Ltd

21

E: [email protected]

T: +44 (0)1638 782600

30

th

Anniversary

Special Offers!

As part of our 30th_{anniversary celebration we are giving}

away a tablet PC to one lucky customer drawn at random every 3 months! Every purchase order received throughout 2013 will

automatically be entered into a prize draw. Orders with email addresses will facilitate the speed of communication. For full terms and conditions please check online at: www.stratech.co.uk/customer-prize-terms For further information visit our website at:

www.stratech.co.uk/offers/customer_prize

Customer PrizeDraw:

Tablet PC Giveaway

Update Your Probe

Subscription

Enter a Tablet PC

Prize Draw!!!

Just go to our subscription page and fill it in accordingly. A winner will be selected at random from all new and updated subscriptions! For further information visit our website at: www.stratech.co.uk/news/probe-prizedraw Closing date 16th_{December 2013}

1983 - 30th Anniversary - 2013

Stratech Focus

We officially opened for business on the 10

th

_{January 1983,}

so throughout 2013 we are celebrating our 30

th

_Anniversary

in style with a new eye-catching special offer every month!

Check them out out:

New Suppliers

Creative Diagnostics is a leading

manufacturer and supplier of antibodies, viral antigens, innovative diagnostic components and critical assay reagents. They provide contract biologic R&D and manufacturing services to diagnostic manufacturers along with GMP Biologics manufacturing for the biopharmaceutical market.

With decades of experience and their unique technologies, Creative Diagnostics prides itself on its commitment to providing customers in research and industry with the highest quality products and services.

Specialities

Diagnostic antibodies, sera and antigens, kits and devices, materials and reagents, custom antibody development services, contract assay development, Omni-HybridomaTM_Platform.

Creative

Research Products

Stratech Scientific

have recently integrated another

member of the CD, Inc. group of companies ‘Creative

Diagnostics’, into our comprehensive portfolio of life

science products, alongside Creative BioMart.

_{RESEARCH AREAS}

Infectious Diseases Cardiovascular Diseases Fertility

Immunology Cancer

Diabetes and Obesity Endocrine/Thyroid Food Safety Allergy Assays Cytokine/Hormone Cell Markers Drugs of Abuse

MORE INFORMATION

ON THESE PRODUCTS AT:

www.stratech.co.uk/creative-diagnostic

or email [email protected]

GBI Labsdevelops and manufactures high quality IHC products for human anatomy pathology, veterinary pathology, antibody screening for drug discovery, and cancer research.

They offer many unique products including; double and triple staining kits for co-expression, same species (Ab tissue) IHC kits, general use/broad range kits, etc. Their mission is to bring state-of-the-art technology and products to researchers and doctors through uncompromised product excellence and knowledgeable technical support.

Their catalogue includes:

Polymer HRP and AP Detection Kits - For human tissue

Polymer HRP and AP Detection Kits - For mouse, rat or other tissue Polymer HRP and AP Detection Kits - Multiple staining for human tissue Polymer HRP and AP Detection Kits - Multiple staining for animal tissue Chromogens

• Chromogen kits for peroxidase

• Chromogen kits for alkaline phosphatase Mounting Mediums

• Mounting medium for IHC and in situ hybridisation with chromogen

• Mounting reagents for fluorescent dyes IHC Buffers/Reagents

• IHC blocking buffers, diluents and other reagents

Antigen Retrieval Buffers Primary Antibodies

Enhanced

Immunohistochemistry

GBI Labs / Golden Bridge

International (GBI), Inc.

was founded in 1993, for

the purpose of supplying

well-developed, affordable

and unique biomedical

reagents to research and

diagnostic communities,

biotech and pharmaceutical

industries across the world.

MORE INFORMATION

ON THESE PRODUCTS AT:

www.stratech.co.uk/gbi-labs

or email [email protected]

New Suppliers

Gene transcription, regulation and the microenvironments surrounding extracellular matrix proteins play an important role in all living cells; enabling them to respond to diverse external

stimuli and to coordinate their inherent cellular programs. By engineering human transcription factor (TF) proteins tagged with a cell membrane penetration domain (CPD), such as 11 arginine (11R tag), LD Biopharma are able to deliver them directly into cells by simply introducing them into culture medium. They can also be efficiently delivered intracellularly using a cationic lipid-mediated system, such as their ProFectin reagent (Catalogue No. R-2000).

Systematically engineering human matrix proteins using refolding technology has allowed them to develop a comprehensive coating/culture system to potentially control stem cell differentiation and many cell lineages in vitroand even in vivo.

Currently, LD Biopharma has more than 325 human cell-type specific recombinant

transcription factor targets under production.

Their recombinant human matrix protein family includes typical matrix proteins, such as vitronectin, E-Cadherin, collagens, lectin proteins, CD and receptor extracellular domains.

Current Product Selection

• Native or 11R tagged recombinant human TF proteins.

• Recombinant Matrix Proteins. • Biomarker Proteins.

• Key enzymatic proteins.

LD Biopharma

specialise

in the production of

human recombinant

proteins. Their core

technology has involved

the development of a

unique temperature shift/

matrix buffer based

E. coli

inclusion body refolding

technology.

Recombinant

Human

TF

and

Matrix Proteins

MORE INFORMATION

ON THESE PRODUCTS AT:

www.stratech.co.uk/ld_biopharma

or email [email protected]

ApexBio originate in Houston, Texas and specialise in producing peptides and lab reagents in house, for biological research. They carry a broad range of products in research areas such as apoptosis,

epigenetics and cancer biology. ApexBio also provide custom services including peptide synthesis, antibody production and assay development.

Recognising the inextricable connection between scientist's tools and their research success, ApexBio pay careful attention to the quality of their products. All products are manufactured with rigorous guidelines and are accompanied with certificates of analysis, HPLC, Mass Spectrum, and H-NMR data.

As part of their signature program, Apexbio offers free samples of newly developed compounds to research scientists who provide feedback after implementing various experiments. For more information on this, please contact [email protected]

Stratech

is happy to

announce our new

agreement with

ApexBio

–

a premier provider of

peptides and lab reagents,

specialising in research

areas such as Apoptosis

and Epigenetics.

Bioanalytic Reagents

–

Apoptosis & Epigenetics

MORE INFORMATION

ON THESE PRODUCTS AT:

www.stratech.co.uk/apexbio or email [email protected]

KEY PRODUCT CATEGORIES

Peptides Apoptosis Epigenetics

Proteasome Inhibitors Protease Inhibitors

New Suppliers

Built on world-class, integrated-chip foundry technology, PhalanxBio

manufactures the OneArray®_{line of}

high-quality and affordable DNA microarrays for gene expression and microRNA profiling. Their expression profiling microarrays have been adopted by core facilities and individual investigators worldwide. In addition, full microarray

services can be attained through their genomics lab where highly-trained specialists provide a comprehensive offering of services including DNA/RNA sample preparation, expression profiling, qPCR validation, and bioinformatic interpretation. Their services and products have contributed to the publication of over 200 manuscripts, and

their manufacturing facility has obtained ISO 9001 and ISO 13485 certification. The USBline of PCR reagents includes a

range of tools for robust and accurate PCR applications. ExoSAP-IT®_{for PCR is a}

unique, single-step, enzymatic cleanup that efficiently removes primers and dNTPs prior to DNA sequencing. USB HotStart-IT®

products provide consistent results at an unmatched value. A variety of other reagents are available for real-time, endpoint and long and accurate PCR. USB enzymes, including polymerases, nucleases, ligases, and DNA binding proteins, are used for molecular biology applications such as amplification, nucleic acid manipulation, and DNA sequencing. With more than 30 years enzyme production experience the quality of the USB enzymes is assured, with all enzymes tested in standard functional assays and certified free of nucleases and proteases. Affymetrix USB also offer a wide selection of products and kits for applications, including cloning, RNA analysis, DNA sequencing, and protein analysis. They offer simple and effective kits for the isolation of nucleic acid and proteins from a variety of starting samples, available in single-column, eight-well strips or plate

formats. Their pre-mixed buffers and solutions give you convenience at the bench and their biochemicals and reagents are of the highest-purity with the proper documentation, every time.

Affymetrix USB

®

Molecular Biology

Reagents

and

Biochemicals

Stratech

is proud to

announce that we have

been appointed the UK and

Irish distributor for the

Affymetrix

USB

®

_brand.

We now offer high quality

USB

®

_{reagents for all your}

research needs. Select

from lab essentials such as

products for PCR,

molecular biology

enzymes, purification kits,

cell signalling tools, and

ultrapure biochemicals.

OneArray

®

DNA MicroArrays

MORE INFORMATION

ON THESE PRODUCTS AT:

www.stratech.co.uk/affymetrix

or email [email protected]

MORE INFORMATION

ON THESE PRODUCTS AT:

www.stratech.co.uk/phalanxbiotech

or email [email protected]

PhalanxBio

provides expression profiling products and

services worldwide to academic, pharmaceutical and

biotechnology researchers.

Special offer!

The First 50Orders will receive a free Photo Frame with Digital Clock for their Lab.

New Suppliers

Our Products

Now On

Your

E-Marketplace!

We support product listings on select E-Procurement platforms; including SciQuest, Science Warehouse and Amici amongst others. If you would like to see Stratech products listed in your institute contact [email protected]

The mission of Signosis is to become a worldwide provider with a broad spectrum of high sensitivity, cost-effective, fast, assay products to target biomarkers with emphasis in a number of diseases including cancers, diabetes/obesity and

cardiovascular diseases.

Products

Cytokine Analysis

- Cytokine ELISA Plate Array

- Human, Mouse and Rat Cytokine ELISA Strips

- Beta Actin, Human, Mouse and Rat Cytokine ELISA Kits

Transcription Factor Assays - TF Activation Profiling Plate Arrays - Promoter-Binding TF Profiling Array - TF Filter Plate Assays

- EMSA (Electrophoretic Mobility Shift Assay) Kits

- Luciferase Reporter Vectors - TF ELISA

Cell Lines and Frozen Cells

- Luciferase Reporter and overexpression Stable Cell Lines

- Transfected Frozen Cells - Cell Arrays

miRNA Research - miRNA Arrays

- miRNA Northern Blot Assay kits - miRNA Luciferase Reporter Vectors - miRNA Real-Time PCR

- miRNA/siRNA and direct Hybridisation Plate Assays

Gene Expression Analysis - Muitiplex PCR Kits

- Single Cell Gene Expression Analysis - cDNA Plate Arrays from RNA - cDNA Plate Arrays from Cell Lysates - esiRNA

Protein Interaction Analysis - Signalling Protein Assays - Co-IP Kits

- Myc Tagged Expression Vectors

Biomarker Analysis

- Beta Actin, Autoimmune, Tumour and Cardiac ELISA Kits

Innovative

Plate Assay Solutions

Stratech Scientific

are delighted to announce a new

partnership with

Signosis Inc,

who are focused on

developing innovative and unique products for biomarker

discovery and analysis in areas of life science research,

clinical application and drug discovery.

MORE INFORMATION

ON THESE PRODUCTS AT:

www.stratech.co.uk/signosis

or email [email protected]

Special offer!

10% Off

all

Signosis

Products

for November

Product Focus

Defective signalling resulting from malfunctioning protein kinases and phosphatases accounts for more than 400 human diseases. These enzymes are "hot" targets for therapeutic interventions. As a leader in Active Enzymes and Signalling Proteins, SignalChem offers a

comprehensive and growing portfolio of Active Kinases & Enzymes (over 500) and the ancillary protein products to help scientists like you to study signalling pathways!

Active

Kinases

&

Phosphatases

FEATURES

Manufacturing and QC in-house High activity

High purity

In vivoactivation

Protein kinases and

phosphatases are key

molecular switches in

cellular signalling

pathways.

PRODUCT NAME

CODE

PRODUCT NAME

CODE

AKT1, Active A16-10G MLCK, Active M62-11G

ALK6 (BMPR1B), Active B05-11G MYLK3, Active M64-10G

CDK5/p25, Active C33-10G P70S6K, Active R21-10H

DCAMKL1, Active D14-10G PDGFR beta, Active P13-11G

EGFR (T790M), Active E10-12EG PDHK4, Active P60-10G

EIF2AK2, Active P80-11G PDK1, Active P14-10H

EIF2AK4 (GCN2), Active E12-11G P13K (p110 beta/p85 alpha), P28-10H

Active

ERK2, Active M28-10G PTEN, Active P23-20G

FAK, Active P91-11H RAF1 (EE), Active R01-13G

FGFR1 (FLT2), Active F04-11G RIPK3, Active R09-10G

GLK, Active M25-11G SPHK1, Active S17-10G

GSK3 beta, Active G09-10H SPHK2, Active S18-10G

HER2, Active E27-11G SRC, Active S19-18G

HPK1, Active M23-11G TBK1, Active T02-10G

IRAK4, Active I12-10G TRKA, Active N16-11G

LRRK2, Active L10-11G TRKB, Active N17-11G

MEKK2, Active M10-10G WNK1, Active W02-11G

Protein Production (Sf9) Proteins are properly folded Proteins are post-translationally modified. Cells are stimulated with growth factors, cytokines, etc. High purity is achieved through propriety processes Enzymes are tested in activity assays Purity is evaluated by densitometry Upstream kinases are co-expressed In vivo Activation Effective

Purification ControlQuality

Figure 1.Protein Production Process

MORE INFORMATION

ON THESE PRODUCTS AT:

www.stratech.co.uk/signalchem

or email [email protected]

Figure 2.Specific activity of SignalChem Active Enzymes, compared to those from

‘competition’. 500 400 300 200 100 0 CK2a1 SignalChem Competitor CHK Src lck Erk1 Zap70 S p e ci fi c A c ti v it y (n m o l/ m in /m g )

AD is a devastating neurological disease that affects more than 37 million people worldwide (1). PD is the second most common neurodegenerative disorder and affects approximately seven million people globally (2,3). The worldwide prevalence of HD is 5–10 cases per 100,000 persons (4,5). As research progresses, many similarities appear which relate these diseases to one another on a sub-cellular level. There are many parallels between different neurodegenerative disorders including atypical protein assemblies as well as induced cell death.(6,7).

Neurodegeneration can be found in many different levels of neuronal circuitry ranging from molecular to systemic. The causes and effects of neuronal degeneration are of major interest to a wide variety of neuroscientists. Paralleling this growing interest is an increasing number of methods applicable to the detection of neuronal degeneration. Biosensis offer Fluoro-Jade®_{C a RTD™}

(Ready-to-Dilute) kit designed to stain for neuronal degeneration, with a fluorescent Nissl counter stain, regardless of specific insult or mechanism of cell death. Fluoro-Jade C exhibits great signal to background noise ratio, as well as high resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localising not only degenerating nerve cell bodies but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols.

The Fluoro-Jade®_{C 'Ready to Dilute' (RTD™)}

Staining Kit provides an easy to use assortment of Fluoro-Jade C, DAPI, sodium

hydroxide and potassium permanganate in liquid form. Fluoro-Jade C labelled degenerating neurons are visualised with blue light excitation while DAPI counter stained cell nuclei are visualised with ultra-violet illumination. The Fluoro-Jade C Staining Kit can be used on all kinds of preserved tissues, including fresh-frozen, paraformaldehyde or formalin fixed, and formalin fixed, paraffin-embedded tissues.

References

1. Alzheimer's Society

-http://www.alzheimers.org.uk/site/scripts/documen ts_info.php?documentID=412 2013

2. Yao, S.C.; Hart, A.D.; Terzella, M.J.(May 2013). "An evidence-based osteopathic approach to Parkinson disease". Osteopathic Family Physician 5(3): 96–101.

3. de Lau LM, Breteler MM(June 2006). "Epidemiology of Parkinson's disease". Lancet Neurol. 5(6): 525–35.

4. Sharon I; Sharon R, Wilkens JP, Ersan T(2010). "Huntington Disease Dementia". emedicine, WebMD. Medscape. Retrieved 2010-05-16.

5. Driver-Dunckley E, Caviness JN.(2007). "Huntington's disease". In Schapira AHV. Neurology and Clinical Neuroscience. Mosby Elsevier. pp. 879– 885. ISBN 978-0-323-03354-1.

6. Rubinsztein DC(October 2006). "The roles of intracellular protein-degradation pathways in neurodegeneration". Nature 443 (7113): 780–6. 2006Natur.443.780R.

7. Bredesen DE, Rao RV, Mehlen P(October 2006). "Cell death in the nervous system". Nature 443(7113):

796–802. 2006Natur.443.796B.

Neurodegeneration is the umbrella term for the

progressive loss of structure or function of neurons,

including death of neurons. Many neurodegenerative

diseases including Alzheimer’s (AD), Parkinson’s (PD),

and Huntington’s Disease (HD) occur as a result of

neurodegenerative processes.

RTD

™

Fluoro-Jade

®

C

staining kit for

identifying neuronal

degeneration

Figure 2. Triple exposure of rat hippocampus exposed to kainic acid. The section was labelled with Fluoro-Jade C and DAPI combined with GFAP IHC. Section shows green Fluoro-Jade C positive neuronal degeneration throughout the entire CA-1 region. Blue viable positive granule cells of the dentate gyrus are DAPI positive. Both regions exhibit red GFAP positive hypertrophied astrocytes. Photo is courtesy of Dr. Larry Schmued/Biosensis

Product Focus

Figure 1.Double exposure of cingulated rat cortex exposed to kainic acid. Layer I contains Fluoro-Jade C positive degenerating axon terminals. Layer II contains densely packed DAPI-positive viable granule cells. Layer III contains Fluoro-Jade C positive degenerating and DAPI-positive viable pyramidal cells.

Photo is courtesy of Dr. Larry Schmued/Biosensis

MORE INFORMATION

ON THESE PRODUCTS AT:

www.stratech.co.uk/biosensis

Product Focus

Proteins often aggregate when exposed to elevated temperatures, long storage periods, repeated freeze/thaw cycles, to high shear forces or intense light. Oxidation by air, reactive reagents or surface exposure provide further sources of protein aggregation.

Protein aggregation can often be avoided with proper choice of pH, salt or

supplementation of stabilising additives in the buffer. This OptiSol protein

solubilisation screening kit contains a systematically varied array of buffers (from pH3 to pH10) and a series of solubility enhancers (salts, amino acids, sugars, polyols, reducing reagents) that enable the determination of conditions under which a particular protein sample is protected from aggregation. Similarly, proteins may be deaggregated by employing such formulations. A total of 90 different formulations with solubility enhancers can be tested in one single, label-free

experiment; included are 6 conditions that function as positive and negative control experiments.

Soluble proteins pass through filters, aggregated proteins don’t. This simple feature is exploited in the filtration step of the OptiSol assay. Those wells that contain soluble proteins will yield protein after the filtration process; those with protein aggregates will not contain any protein.

After running the OptiSol Protein Solubilisation kit, you have established conditions that keep your protein target in solution and you know conditions at which your protein aggregates.

If your challenge is:

• Undesired antibody or protein aggregation • Reversible precipitation of proteins during

elution off a purification column • Insoluble protein at neutral pH If you need to:

• Screen different buffers to maintain your protein stable at high concentrations. • Keep an antigen soluble while removing

certain components from the buffer • Separate bound antigen-antibody complex

into unbound forms

• Find optimal conditions for long term stability of proteins following purification • Optimise purification conditions

The OptiSol Protein Solubility Screening kit could take

you

a huge step forward.

Find Your Protein’s

Solubility Sweet Spot:

OptiSol

BENEFITS:

Cost Effective:

A simple-to-use kit, which consumes minimal protein that can be used with standard laboratory equipment.

Better:

Less batch-to-batch variation between protein preparations, higher purity, improved reproducibility and consistent activity.

Faster:

Succeed with your first protein preparation. No more additional purifications necessary because protein preparations are not ‘crashing out’ any more as a result of protein aggregation.

Smarter:

The Protein Dashboard™ supports spotting trends and identifying critical solvent factors for optimal protein stability.

OptiSol

Protein Solubility

Kits help researchers

identify formulations that

protect a target protein

from aggregation. They

can also be used to

solubilise an already

aggregated protein

sample.

MORE INFORMATION

ON THESE PRODUCTS AT:

www.stratech.co.uk/dilyx

or email [email protected]

Special offer!

10% Off for New

Customers

Product Focus

The system, developed by System Biosciences (SBI) uses a nuclease, CRISPR-associated (Cas9) that complexes with small RNA’s as guides (gRNAs) to cleave DNA in a sequence-specific manner upstream of the Protospacer Adaptor Motif (PAM) in any genomic location. The CRISPR-Cas9 system is comprised of separate guide RNAs known as the crRNA and tracrRNA. These two separate RNAs have been combined into a single RNA to enable site-specific mammalian genome cutting through the design of a short guide RNA. SBI has built a series of all-in-one CRISPR-Cas9 cloning vectors with different promoter options. These cloning vectors will express a human codon-optimised Cas9 nuclease as well as any gRNA

sequence of your choice linked to the tracrRNA. Simply design the gRNA for the genome target DNA sequence and clone into the Cas9 SmartNuclease™ vector for efficient genome engineering.

How to build a Cas9 SmartNuclease™ Vector

The general workflow of the cloning, validation, and transfection of the

gRNA/Cas9 expression construct into cells is simple. First, design two DNA

oligonucleotides that are sense and antisense sequences of the target DNA, which is 20 bp upstream of the Protospacer Adaptor Motif PAN (5’ – NGG – 3’) (Figure 1). Anneal the two oligonucleotides to

generate a duplex and clone the duplex directly into the provided linearised CRISPR-Cas9 vector by ligation (Figure 2). Transform into competent cells and grow

the clones using LB/Kanamycin. Confirm the correct clones by plasmid construct sequencing.

Highlights:

• Specific genome cleavage guided by an RNA sequence

• Easy gene knockouts

• Efficient genome editing with homologous recombination

• No need to make a new protein effector for each new target

• All-in-one Cas9-gRNA cloning and expression vectors

The recent discovery of

the type II prokaryotic

CRISPR (Clustered

Regularly Interspaced

Short Palindromic Repeats)

system in the bacterium

Streptococcus pyogenes

has provided a

revolutionary tool for

targeted genome

engineering, with simple

elegance.

PrecisionX

™

Cas9 SmartNuclease

™

System from

SBI

MORE INFORMATION

ON THESE PRODUCTS AT:

http://www.stratech.co.uk/sbi or email [email protected]

Figure 2.RNA-directed Cas9 Nuclease Expression Vectors

Figure 1.The CRISPR-Cas9 Nuclease Heterocomplex

PRODUCT

PACK SIzE

CATALOGUE

EF1-hspCas9-H1-gRNA linearised SmartNuclease vector 10 reactions CAS900A-1-SBI

CAG-hspCas9-H1-gRNA linearised SmartNuclease vector 10 reactions CAS920A-1-SBI

CMV-hspCas9-H1-gRNA linearised SmartNuclease vector 10 reactions CAS940A-1-SBI

MSCV-hspCas9-H1-gRNA linearised SmartNuclease vector 10 reactions CAS960A-1-SBI

PGK-hspCas9-H1-gRNA linearised SmartNuclease vector 10 reactions CAS980A-1-SBI

Cas9 SmartNuclease AAVS1-gRNA Targeting Vector (EF1a promoter) 10 µg CAS601A-1-SBI

Cas9 SmartNuclease AAVS1 Positive control kit (includes CAS601A-1, 1 kit CAS605A-1-SBI

Product Focus

Technology from HepatoChem™ exploits an optimised panel of catalytic chemical reaction conditions in a multi-well parallel format, which by its diversity, mimics the suite of cytochrome P450 enzymes (CYP) present in human hepatocytes, offering the researcher a unique, synthetic chemical liver.

Exciting new technology speeds up metabolite study.

• Produce drug metabolites in days, not months.

• Exclusive process produces large quantities of metabolites.

• Rapid, inexpensive scale up for NMR and

in vitrotesting.

• Eliminate complex and costly synthetic chemistries.

HepatoChem™ Technology

Their innovative approach is based on a diverse set of chemical conditions that mimics the main reactions occurring during drug metabolism. This panel is used to quickly find reaction conditions that

generate specific drug metabolites. This approach (basically a chemical liver), is analogous to the panels of cytochromes P450 present in the human liver, but with the advantage of the scalability of chemistry. When a reaction condition is identified, it is optimised and scaled up to produce milligram to gram quantities of metabolites.

HepatoChem™ has developed a series of biomimetic panels. These panels use several kinds of catalysts, including metalloporphyrins, which gives access to a unique set of reaction conditions. This series of easy-to-use kits is suitable for the preparation of metabolites for MS and NMR.

Key Benefits

Direct synthesis of Metabolites from

•

parent drug

Authentication with biological

•

reference samples

Rapid optimisation in hours

•

Milligram to gram scale

•

Common Biotransformation Reactions

•

• Aliphatic Hydroxylation • N-Dealkylation • O-Dealkylation • Cleavage • N-Oxidation • S-Oxidation • Rearrangement • Formulation

MetaboLyte™ Biomimetic Oxidation Kits

Three kits have been introduced to help the researcher produce metabolites for their drug candidates. These kits perform a one-step synthesis of metabolites from the parent drug.

• Fast • Inexpensive • Reproducible

• Scalable from μg to mg to g

• Convenient room temperature reagents • Easy-to-use

US Biological and fellow Massachusetts company

HepatoChem™

have joined forces to offer a

revolutionary way to screen, optimise and produce

metabolites from drug candidates.

MORE INFORMATION

ON THESE PRODUCTS AT:

www.stratech.co.uk/usbio or email [email protected]

MetaboLyte

™

Production Platform

-From Screening To

Optimisation To

Production

A B C D E F G H A B C D E F G H A B C D E F G H 1 2 3 4 5 6 7 8 9 10 11 12 1 2 3 4 5 6 7 8 9 10 11 12 1 2 3 4 5 6 7 8 9 10 11 12 Optimisation Kit M3008-20 Production KitM3008-30

Complete Kit includes all solvents and reagents for 2x22 screening reaction conditions. 2 plates included

1

Perform the primary screen. Select desired metabolite wells. Order corresponding optimisation kit.

2

Perform the optimisation kit. Identify best production conditions. Order corresponding production kit.

3

Scale-up and isolate your metabolite.

Complete Kit includes all solvents and reagents needed to produce your metabolite 0.01 mmol scale.

Complete Kit includes all solvents and reagents for optimisation of selected screening reaction conditions. 1 plate included.

Product Focus

Antigen detection with primary antibodies of the same species as the test tissue yields high background when using an indirect detection method. This severely limits the use of mouse monoclonal antibodies on mouse tissues. GBI Labs' ultra sensitive polymer HRP and AP kits use the latest innovation in polymer conjugated secondary antibody together with

proprietary blocking buffer to detect mouse antibody on mouse tissue, rat antibody on rat tissue, rat antibody on mouse tissue, or mouse antibody on rat tissue with very clean background.

Mouse Antibody On Mouse Tissue

The Klear Mouse HRP-Polymer Detection System is designed for staining mouse antibodies on mouse tissues. The kit uses a special blocking buffer, polymeric HRP-linked secondary antibody as well as mouse antibody enhancer to detect mouse primary antibody that is bound to the mouse tissue. This technology provides excellent

sensitivity and high specificity. Being a biotin-free system, it overcomes the nonspecific staining caused by the streptavidin/biotin system due to endogenous biotins.

Features

• Polymer detection with super sensitivity. Biotin free.

• Specially designed to detect MOUSE IgG antibody on MOUSE tissue

• Clean background.

• Simple procedure with only 35 minutes of blocking step

• Select chromogen Kits: DAB, DAB-Ni, AEC, AP-Red+, Fast-Red, BCIP/NBT, AP-Blue.

Mouse Antibody On Rat Tissue

Detecting mouse primary antibody on rat tissue is a very difficult task due to background staining issues. Polink-2 Plus HRP Mouse-NR (no cross reactivity to rat) DAB Detection Kit is specially designed to solve this problem. The secondary antibody is adsorbed to rat, rabbit and human serum proteins, providing excellent specificity to detect mouse primary antibody on rat tissue. Besides rat tissue Polink-2 Plus HRP Mouse-NR DAB Detection kit also can be used on human tissue and rabbit tissue. Polink-2 Plus HRP Mouse-NR DAB Detection Kit is the 3rd_{generation of polymer}

detection system. It uses mouse antibody enhancer to help amplify the polymer-enzyme conjugate reaction to achieve super sensitivity and specificity in IHC staining. It produces consistent immunostaining outcomes on archival tissues and on difficult-to-work with antibodies. Most commonly used specimens for this system are: frozen tissue, paraffin-embedded tissue, freshly prepared

lymphocytes and fixed culture cells. It can be used for manual or auto-staining.

Features

• Two steps polymer detection system (3rd

generation technology) with super sensitivity. Biotin free.

• Specially designed to detect MOUSE IgG antibody on RAT tissue

• Clean background.

• 10 + 10 minutes incubation.

• Human, rat, and rabbit serum protein adsorbed.

• Select chromogen Kits: DAB, DAB-Ni , AEC, Red+, Fast-Red, BCIP/NBT, AP-Blue

Kits available to trial are listed at the top of the page.

Please contact [email protected] for more details and for a more comprehensive list of all other samples available.

Immunohistochemistry

- Same Antibody &

Tissue Species

MORE INFORMATION

ON THESE PRODUCTS AT:

www.stratech.co.uk/gbi-labs or email [email protected]

D50-6-GBI-SAMPLE Klear mouse AP with Fast Red Kit SAMPLE Primary Antibody Antigen– Mouse

Tissue – Mouse/rat

D51-6-GBI-SAMPLE Klear Mouse AP with AP-Red+ Kit SAMPLE Primary Antibody Antigen – Mouse

Tissue – Mouse/rat

D52-6-GBI-SAMPLE Klear Mouse HRP with DAB Kit SAMPLE Primary Antibody Antigen– Mouse

Tissue – Mouse/rat

D53-6-GBI-SAMPLE Klear Mouse HRP with AEC Kit SAMPLE Primary Antibody Antigen– Mouse

Tissue – Mouse/rat

D58-6-GBI-SAMPLE Polink-2 Plus HRP Mouse-NR with DAB Kit SAMPLE Primary Antibody Antigen– Mouse

Product Focus

EdU / EU CLICK Reagents – an alternative to BrdU/BrU

Traditionally, incorporation of 5-Bromo (deoxy) uridine (5-BrdU/5-BrU) into nascent DNA or RNA strands has been employed to assess proliferation. This method is hampered by the cell morphology destructive detection via a Br(d)U specific antibody that requires harsh cell

permeabilisation conditions and nucleic acid denaturation procedures preventing subsequent analysis of other cellular targets.

This drawback can be overcome by the incorporation of ethynyl-labelled (deoxy)uridine (5-EdU & 5-EU) and the subsequent detection of the ethynyl-functionalised DNA/RNA by covalent conjugation of small-sized fluorescent azides.

5-EdU & 5-EU-based nucleic acid labelling;

• is non-destructive and therefore suitable for mulitparametric analysis (no harsh permeabilisation & nucleic acid denaturation required).

• provides a significantly faster detection procedure (∼2h instead of > 4h). • is compatible with flow cytometry &

microscopy

• is compatible with many cell lines and organisms

CytoTell™ – An alternative to CFSE

Among all the existing fluorescent dyes, CFSE is the preferred cell proliferation indicator that is widely used for live cell analysis. However, there are a few major drawbacks associated with the use of CFSE for monitoring cell proliferation.

• CFSE cannot be used with GFP labelled cells or FITC channel labelled antibodies • CFSE is highly toxic to cells since it

indiscriminately reacts with all amino groups.

• CFSE has slow response and is inconvenient to use. The CFSE fluorescence intensity of the 2nd

generation cells is decreased more than 10 fold from the 1st_{generation. You would}

have to wait for another generation to start the cell proliferation analysis;

• Medium removal is required for analysis by Flow Cytometry as CFSE reacts with medium components.

The CytoTell™ Dyes have been developed to eliminate the above CFSE limitations and are available with Red, Green and Blue spectra for ultimate flexibility

CytoTell™ dyes can also be used for long term tracking of labelled cells and are compatible for use with standard formaldehyde-containing fixatives and saponin-based permeabilisation buffers for additional/antibody based staining. CytoTell™ Dyes provide;

• Much faster response to cell proliferation than CFSE.

• Greater convenience than CFSE. • Increased sensitivity over CFSE.

Also Available Through Stratech:

Incorporation assays: BrdU, and other labelled nucleotides (e.g. Fluorescent, Biotin & Digoxin)

Colorimetric Assays: MTT, xTT, WST (1, 3, 5 & 8)

Cell based dyes and stains, e.g. CFSE, DAPI, Hoechst, etc.

Wide range of secondary detection reagents: Fluorescently labelled azides & streptavidin, Antibodies against BrdU, Biotin & Digoxin.

Cell Proliferation

Assays

Assaying cell proliferation is central to the work

undertaken in many research laboratories and having a

variety of high quality, reliable and effective reagents

available is vital. Stratech offer a wide range of

reagents and assays from a variety of manufacturers

around the World at incredibly competitive prices. Below

we focus on the alternatives that we offer as

replacements to BrdU and CFSE based assays, more

reagents & kits can be found on our website.

MORE INFORMATION

ON THESE PRODUCTS AT:

www.stratech.co.uk or email [email protected]

Figure 1.Cell tracking assay with CytoTell™ Green and CFSE. Jurkat cells (~2x106 cells/ml) were stained with CytoTell™ Green or CFSE (0.5 μM) on Day 0. The cells were passed serially at 1:1 ratio for 9 days. Fluorescence intensity measured in the day after passage

NAME CAT NO. Ex(NM) EM(NM)

CytoTell™ 22255-ABD 630 660 Red CytoTell™ 22253-ABD 511 525 Green CytoTell™ 22251-ABD 403 454 Blue 100 80 60 40 20 0 0 1 2 3 4 10 10 FL1-H CytoTell™ Green Unstained Cells Day 0 Day 1 Day 2 Day 3 Day 4 Day 7 Day 8 Day 9 CFSE

Big gap between 1st and 2nd generation 10 10 10 C o u n ts 100 80 60 40 20 0 0 1 2 3 4 10 10 FL1-H 10 10 10 C o u n ts

Product Focus

Cytokines are signalling molecules that have critical roles in many biological processes such as cellular growth, differentiation, gene expression, migration, immunity and inflammation. Their malfunction leads to many diseases, including arthritis, acute and chronic liver disease, inflammatory bowel disease, cardiac-related diseases and cancers. Cytokines are commonly working together in a biological or disease process,

therefore, the comprehensive analysis of the expression of multiple cytokines is important in revealing the mechanisms underlying many biological functions and diseases.

The Human Cytokine ELISA Plate Array from Signosis is available with chemiluminescent or colourimetric detection that allows you to monitor the abundance of 31 human cytokines simultaneously. This fast and sensitive assay can be used for quantitative comparison of these cytokines among different samples.

The 96-well white plate is divided into 3 or 4 sections, and each section has 4 or 3 columns for one sample. In each section, 31 specific cytokine capture antibodies are coated on 31 wells and one blank. The sample can be cell culture supernatants, cell lysates, tissue homogenates, serum, or plasma samples among others.

Mouse and rat cytokine arrays are also available please contact

[email protected] for futher information.

MORE INFORMATION

ON THESE PRODUCTS AT:

www.stratech.co.uk/signosis or email [email protected]

1 2 3 4 5 6 7 8 9 10 11 12

A TNFa VEGF PDGF-BB IL-10 TNFa VEGF PDGF-BB IL-10 TNFa VEGF PDGF-BB IL-10

B IFNr EGF PIGF-1 FGFb IFNr EGF PLGF-1 FGFb IFNr EGF PLGF-1 FGFb

C G-CSF IL-6 b-NGF Leptin G-CSF IL-6 b-NGF Leptin G-CSF IL-6 b-NGF Leptin

D GM-CSF Resistin SCF IGF-1 GM-CSF Resistin SCF IGF-1 GM-CSF Resistin SCF IGF-1

E IL-1a PAI-1 MCP-1 TGF-b IL-1a PAI-1 MCP-1 TGF-b IL-1a PAI-1 MCP-1 TGF-b

F IL-8 IL-12 MIP-1a Adipo IL-8 IL-12 MIP-1a Adipo IL-8 IL-12 MIP-1a Adipo

G IP-10 IL-13 IL-2 IL-17a IP-10 IL-13 IL-2 IL-17a IP-10 IL-13 IL-2 IL-17a

H Rantes Eotaxin-3 IL-4 Blank Rantes Eotaxin-3 IL-4 Blank Rantes Eotaxin-3 IL-4 Blank

BENEFITS:

Multiplex – One pre-coated plate allows the measurement of: 31 cytokines for 3 human samples.

High Sensitivity – Requires minimal amount of samples.

Quantitative profiling– Allows quantitative comparison of expression among samples.

Product Name

Sizes

Catalogue No.

Human Cytokine ELISA Plate Array 3 Reactions EA-4001

(Chemiluminescence)

Human Cytokine ELISA Plate Array 3 Reactions EA-4002

(Colorimetric)

Human Cytokine

ELISA Plate Arrays

1983 - 30th Anniversary - 2013

Stratech Focus

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THE

PROBE

Who are we

Stratech Scientific Ltd. provides over 750,000 innovative and specialist life science research products. We have built an excellent reputation over 30 years for supplying high quality, competitively priced, reliable products to our end users.

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October 10 2013

Edinburgh University Supplier’s Fair Edinburgh UK

November 1 2013

Leeds University Flow Cytometry Conference

Leeds UK

December 2-5 2013 BSI Annual Congress Liverpool UK De sig ne d an d pr in te d by : m jw eb ba ss oc ia te s l td | N ew m ar ke t