• No results found

Basic fibroblast growth factor as one of the essential factors regulating lens transdifferentiation of pigmented epithelial cells

N/A
N/A
Info
Download
Protected

Academic year: 2020

Share "Basic fibroblast growth factor as one of the essential factors regulating lens transdifferentiation of pigmented epithelial cells"

Copied!
8
0
0

Loading.... (view fulltext now)

Download now ( 8 Page )

Full text

(1)
(2)
(3)
(4)
(5)
(6)
(7)
(8)

Figure

Fig. 1. SDS-PAGEof purifiedovine testisHUase.HUasewas purifiedfrom a commercial preparation of HUase(BoehringerMannheim,Lot.11366220-24)by a two-stepcolumnchromatographyprocedure.85 de-scribedin MaterialsandMethods.(LanePJ PurifiedHUasewith anapparent MW 6
Fig. 3. Heparin-Sepharosechromatography.andOne hundred mg of crude HUase (Sigma. Lot. 79F-0098J was applied to a heparm-Sepharosecolumn (10mm;o..20 mm) eqUIlibratedwith 10 mM sodium phosphatebuffer (pH 7.4) containing 0.8 M NaC/
Fig. 5. SDS.PAGEof purified18 kDa protein.The 18 kDa proteinin theboundfractionII fromthe heparinaffinitycolumnwas furtherpurifiedbyreversed-phaseHPLC
Fig. 7. Effectof basicFGF on lens transdifferentiation.PECs weremaintainedfor30 days with EdFP medium containing basic FGf at aconcentrationof 1, 5, 10 or 30 ng/mf

References

Download now ( PDF - 8 Page - 3.52 MB )

Related documents