Ranjan K. Dash, PhD
Professor of Biomedical Engineering and Physiology Medical College of Wisconsin, Milwaukee, WI
OFFICE ADDRESS: Department of Biomedical Engineering Room #: TBRC-C2910
Medical College of Wisconsin 8701 Watertown Plank Road Milwaukee, WI 53226-6509 Phone: (414) 955-4497 Fax: (414) 955-6546 E-mail: [email protected]
Website: http://mcw.marquette.edu/biomedical-engineering/ranjan-dash.php HOME ADDRESS: 5614 S. Red Fox Rd.
New Berlin, WI 53151-7316 Phone: (414) 367-2679 (home)
(414) 861-9501 (mobile) PLACE OF BIRTH: Jagatsinghpur, Orissa, India
CITIZENSHIP: USA (effective 2/2015); USA green card holder (6/2007 – 2/2015);
USA nonimmigrant visa holder (J1 and H1B) (8/1998 – 6/2007) EDUCATION:
7/1985 – 6/1988: BSc, Mathematics, Utkal University, Bhubaneswar, Orissa, India 7/1988 – 6/1991: MSc, Mathematics, Utkal University, Bhubaneswar, Orissa, India 12/1992 – 7/1998: PhD, Theoretical Cardiovascular Fluid Dynamics, Indian Institute
of Technology, New Delhi, India
Thesis: Theoretical Studies of Cardiovascular Flows in Small and Large Blood Vessels
POSTGRADUATE TRAINING AND FELLOWSHIP APPOINTMENTS:
12/1992 – 12/1994: Junior Research Fellow and Teaching Assistant, Department of Mathematics, Indian Institute of Technology, New Delhi, India Advisors: Drs. Girija Jayaraman and Kamal Mehta
12/1994 – 7/1998: Senior Research Fellow and Teaching Assistant, Department of Mathematics, Indian Institute of Technology, New Delhi, India Advisors: Drs. Girija Jayaraman and Kamal Mehta
1/1996 – 7/1998: Research Scientist, Department of Mathematics, Indian Institute of Technology, New Delhi, India
Advisors: Drs. Girija Jayaraman and Kamal Mehta
9/1998 – 7/2000: Postdoctoral Research Associate, Department of Mathematics, Texas A&M University, College Station, TX
Advisor: Dr. Prabir Daripa
10/2000 – 11/2002: Postdoctoral Senior Fellow, National Simulation Resource for Circulatory Mass Transport and Exchange, Department of Bioengineering, University of Washington, Seattle, WA
Advisor: Dr. James Bassingthwaighte; Collaborator: Dr. Daniel Beard 12/2002 – 8/2003: Postdoctoral Senior Fellow, Resource Facility for Population Kinetics,
Department of Bioengineering, University of Washington, Seattle, WA Advisor: Dr. Paolo Vicini; Collaborators: Drs. James Bassingthwaighte,
Daniel Beard, and Martin Kushmerick
9/2003 – 5/2006: Senior Research Associate, Departments of Pediatrics and Biomedical Engineering, Center for Modeling Integrated Metabolic Systems (MIMS), Case Western Reserve University, Cleveland, OH
Advisors: Drs. Marco Cabrera and Gerald Saidel; Collaborators: Drs.
Daniela Calvetti and Erkki Somersalo FACULTY APPOINTMENTS (INCLUDING SECONDARY AND ADJUNCT APPOINTMENTS):
6/2006 – 6/2010: Assistant Professor, Department of Physiology and Biotechnology and Bioengineering Center, Medical College of Wisconsin, Milwaukee, WI 7/2010 – 6/2016: Associate Professor, Department of Physiology and Biotechnology and
Bioengineering Center, Medical College of Wisconsin, Milwaukee, WI 11/2013 – 6/2016: Adjunct Associate Professor, Department of Biomedical Engineering,
Marquette University, Milwaukee, WI
7/2016 – 6/2018: Associate Professor (Primary) and Co-Director of Graduate Studies, Department of Biomedical Engineering, Marquette University and Medical College of Wisconsin, Milwaukee, WI
7/2016 – 6/2018: Associate Professor (Secondary), Department of Physiology, Medical College of Wisconsin, Milwaukee, WI
8/2016 – 7/2018: Adjunct Associate Professor, Department of Biomedical Engineering, College of Engineering & Applied Science, University of Wisconsin- Milwaukee, WI
7/2018 – Present: Professor (Primary) and Co-Director of Graduate Studies, Department of Biomedical Engineering, Marquette University and Medical College of Wisconsin, Milwaukee, WI
7/2018 – Present: Professor (Secondary), Department of Physiology, Medical College of Wisconsin, Milwaukee, WI
8/2018 – Present: Adjunct Professor, Department of Biomedical Engineering, College of Engineering & Applied Science, University of Wisconsin-Milwaukee, WI
FACULTY AFFILIATIONS WITH MCWRESEARCH CENTERS:
6/2006 – 6/2016: Biotechnology and Bioengineering Center (Director: Andrew Greene) 9/2011 – 8/2013: Center for Computational Medicine and Virtual Physiological Rat Center
(Director: Daniel Beard)
12/2014 – Present: Cardiovascular Research Center (Director: Ivor Benjamin) 12/2015 – Present: Center of Systems Molecular Medicine (Director: Mingyu Liang)
AWARDS AND HONORS:
6/1988: Graduated BSc in First Class Honors and Distinction from Utkal University, Bhubaneswar, Orissa, India
6/1991: Graduated MSc in First Class with 4th Rank (81%) from Utkal University, Bhubaneswar, Orissa, India
12/1991: Qualified National Eligibility Test (NET) for Junior Research Fellowship conducted jointly by the Council of Scientific and Industrial Research and University Grant Commission, Govt. of India
12/1992 – 12/1993: Secured a high 9.7 CGPA on 10-point scale from 25 credits in pre-PhD course work at the Indian Institute of Technology, New Delhi, India
12/1992 – 12/1994: Junior Research Fellowship, University Grant Commission, Govt. of India 12/1994 – 12/1997: Senior Research Fellowship, University Grant Commission, Govt. of India 1/1996 – 7/1998: Project Scientist on a sponsored research project “Flow through Pipes and
Channels of Variable Cross Sections” (PI: Girija Jayaraman) from Department of Science and Technology, Govt. of India
7/2007 – 6/2011: Scientific Development Grant ($260,000/4 Years) from American Heart Association (National Affiliate)
12/2010: Marquis Who’s Who in Science and Engineering for the year 2011-2012 (for noteworthy achievements that are of significant value to the society) 9/2011: Founding Faculty, Virtual Physiological Rat Center (an NIH-funded National
Center for Systems Biology) and Center for Computational Medicine, Medical College of Wisconsin. The Center was closed in MCW with the PI/PD Dr. Daniel Beard moving to University of Michigan (8/2013)
8/2015: Outstanding Faculty Service Award for service to the Library Committee (July 2014 – June 2015), Medical College of Wisconsin
12/2017: Dean’s Choice Outstanding Graduate School Educator Award for the academic year 2016-2017 for commitment to excellence in graduate education, Graduate School of Biomedical Sciences, Medical College of Wisconsin
7/2018: Award of Tenure for distinction in scholarly and intellectual achievements, Department of Biomedical Engineering, Marquette University and Medical College of Wisconsin
12/2020: Dean’s Choice Outstanding Graduate School Educator Award for the academic year 2019-2020 for commitment to excellence in graduate education, Graduate School of Biomedical Sciences, Medical College of Wisconsin
MEMBERSHIPS IN HONORARY AND PROFESSIONAL SOCIETIES:
1/2002 – Present: Professional Member, Biomedical Engineering Society 10/2008 – Present: Professional Member, Biophysical Society
10/2008 – Present: Professional Member, American Physiological Society (Affiliated to Cardiovascular Section)
10/2011 – Present: Professional Member, American Heart Association (Affiliated to Council on Basic Cardiovascular Sciences)
10/2016 – Present: Professional Member, IEEE Engineering in Medicine and Biology Society EDITORSHIPS/EDITORIAL BOARDS/JOURNAL REVIEWS/CONFERENCE SESSIONS:
Editorships and Editorial Board Memberships:
3/2009 – Present Associate Editor, IEEE EMBS Conference Proceedings (Topics: Systems Biology;
Bioinformatics and Computational Biology; and Modeling Methodologies) Workload involved handling of 28 papers in 2009, 24 papers in 2010, 22 papers in 2011, 20 papers in 2012, 18 papers in 2013, 16 papers in 2014, 21 papers in 2015, 18 papers in 2016, 15 papers in 2017, 10 papers in 2018, 15 papers in 2019, and 15 papers in 2021. This includes finding reviewers, assigning papers to the reviewers, getting reviews done for all the papers within stipulated time, reviewing papers if needed, writing review reports, and recommending papers for presentation and publication (occurs around March-June of each year) 9/2010 – Present: Editorial Board Member, Journal of Bioengineering and Biomedical Science
(Hilaris Publisher) and Bioenergetics: Open Access (OMICS Publishing Group) 3/2011 – Present: Reviewing Editor of Systems Biology associated with Frontiers Journals
(Frontiers in Physiology; Frontiers in Applied Mathematics and Statistics) 5/2014 – Present: Reviewing Editor of Cardiovascular Genetics and Systems Medicine, Frontiers
in Cardiovascular Medicine
11/2017 – Present: Contributing Faculty Member of F1000Prime, Integrative Physiology Section of F1000 Physiology
3/2021 – Present: Associate Editor of Computational Physiology and Medicine, Frontiers in Physiology
Journal Manuscript Reviewing:
Manuscript Reviewer for the following international scientific journals:
2002 – Present (various dates):
■ American Physiological Society Journals: AJP Heart and Circulation Physiology; AJP Cell Physiology; AJP Endocrinology and Metabolism;
AJP Regulatory, Integrative and Comparative Physiology; Journal of Applied Physiology
■ Biochimica et Biophysica Acta Journals: BBA Bioenergetics; BBA Biomembrane; BBA General Subject; BBA Molecular Cell Research
■ Biomedical Engineering Society Journals: Annals of Biomedical Engineering; Cardiovascular Engineering and Technology; Cellular and Molecular Bioengineering
■ Biomedical Central Journals: BMC Biophysics; BMC Physiology; BMC Systems Biology; Biomedical Engineering Online
■ Miscellaneous Physiology, Biophysics, and Biochemistry Journals:
Biophysical Journal; Bioscience Report; Chemical Science; Cell Biophysics and Biochemistry; European Journal of Pharmacology; Frontiers Journals (as Reviewing Editor); Free Radical Biology and Medicine; Nitric Oxide;
Journal of Physiology London; Journal of Biological Chemistry
■ Miscellaneous Computational Biology and Medicine Journals: Computers in Biology and Medicine; Computational Biology and Chemistry; IEEE EMBS Conference Proceedings (Associate Editor); IET Systems Biology; IMA Journal of Mathematical Medicine and Biology; Journal of Royal Society Interface;
Mathematical Biosciences; Mathematical and Computer Modeling;
Mathematical Problems in Engineering; PLoS One; PLoS Computational Biology; Theoretical Biology and Medical Modeling
■ Biofluid Dynamics Journals: Acta Mechanica; Acta Biotheoretica; Journal of Applied Fluid Mechanics; Physica-A; Physica-D
Conference Abstract Reviewing and Poster Judging:
2015, 2016: Abstract Reviewer, Proceedings of the Annual Biomedical Engineering Society Meetings (20-25 abstracts/year during summer); Poster Presentations Judge (8-10 posters/year), Annual Biomedical Engineering Society Meetings 2020: Abstract Reviewer, Proceedings of the Society of Free Radicals in Biology and
Medicine (SFRBM) Meetings (20-25 abstracts/year during fall);
Workshops/Symposiums/Conference Sessions Organizing/Chairing:
6/2007: Co-organized and co-chaired with Dr. Dexuan Xie (University of Wisconsin- Milwaukee) a special oral session on “High Performance Computing for Biological Modeling and Simulations” at the 7th International Conference on Computational and Mathematical Methods in Science and Engineering (CMMSE-2007), Chicago, June 20-23, 2007
Organizing Committee Member of the 2013 and 2017 Cardiac Physiome Society Annual Meetings held in Bar Harbor/Maine/USA (Oct 2013) and Toronto/Canada (Nov 2017). Involved in suggesting, discussing, deciding on the potential invited speakers, and in other activities
6-10/2013, 6-10/2017:
10/2016: Chaired (Co-chair: Stacey Finley, University of Southern California) a platform session on “Metabolic Models”, 2016 Biomedical Engineering Society Annual Meeting, Minneapolis, Oct 5-8, 2016
10/2016: Chaired (Co-chair: Said Audi, Marquette University) a platform session on
“Computational Modeling in Cardiovascular Systems I”, 2016 Biomedical Engineering Society Annual Meeting, Minneapolis, Oct 5-8, 2016
NATIONAL/INTERNATIONAL ELECTED/APPOINTED LEADERSHIP AND COMMITTEE POSITIONS: National/International Grant Reviewing Committees:
5/2010: Member, NIH/CSR Special Emphasis Study Panel ZHL1 CSR-G (S1) for RFA-HL- 10-017 “Functional Modeling of Pediatric Upper Airway Disorders (R01)”;
Reviewed 5 R01 grants
12/2010: International Reviewer of a computational biofluid dynamics grant from the National Foundation of Science, Higher Education and Technological Development of the Republic of Croatia
3/2012 – 3/2016: Member, AHA Bioengineering and Basic Science Study Panel (Vascular Biology, Cardiac Mechanics, Bioengineering); Reviewed 10 grants/year
6/2012, 10/2013: Member, NIH/CSR Special Emphasis Study Panel ZEB1 OSR-C (O1) for MSM PAR- 11-203 “Predictive Multiscale Models for Biomedical, Biological, Behavioral, Environmental and Clinical Research (Interagency U01)”; Reviewed 4 U01 grants in 6/2012 and 3 U01 grants in 10/2013
7/2015: Member, NIH/CSR Special Emphasis Study Panel ZRG1 BST-T (O2) for Bioengineering Sciences and Technologies; Reviewed 4 R01 grants
10/2018: International Reviewer of a computational modeling grant focusing on cellular metabolism and tumor progression from the Netherlands Organization for Scientific Research, Earth and Life Sciences Division
10/2019: International Reviewer of a computational modeling grant focusing on cellular metabolism using a telecommunication approach from the National Science Centre of Poland
7/2020: Member, NIH/CSR Special Emphasis Study Panel ZRG1 CVRS A05 (Cardiovascular Respiratory Sciences); Reviewed 3 R01 grants
National/International PhD Dissertation Reviewing Committees:
4/2008: Invited International Reviewer of PhD dissertation “The Effect of Chaotic
Vasomotion in Skeletal Muscle on Tissue Oxygenation” of Mr. Ranjan K. Pradhan (Advisor: Dr. V. S. Chakravarthy), Dept. of Electrical Engineering, Indian Institute of Technology, Chennai, India
8/2016: Invited International Reviewer of PhD dissertation “Enhanced 1D Modeling for Predicting BID-DO Conditions in River” of Ms. Ritu Kapoor (Advisor: Dr. Babita 11 /2019, 3/2021; Co-Chair, AHA Fellowship Bioengineering and Basic Science Peer Review Panel 11/2021: (Vascular Biology, Cardiac Mechanics, Bioengineering)
Tyagi), Dept. of Mathematics, Banasthali University, Uttar Pradesh, India 1/2018: Invited International Reviewer of PhD dissertation “Some Mathematical Models
of Flow in Renal Tubules and Solute Transfer in Glomerular Capillaries” of a graduate student of Dr. P. Muthu, Dept. of Mathematics, National Institute of Technology (NIT), Warangal, Telangana, India
1/2019: Invited International Reviewer of PhD dissertation “Role of Ultrastructural Alterations in Diabetic Cardiomyopathy” of Mr. Shouryadipta Ghosh (a graduate student of Dr. Vijay Rajagopal), Dept. of Biomedical Engineering, University of Melbourne, Australia
National/International Faculty Promotion Reviewing Committees:
4/2012: Invited External Reviewer of Dr. Ponakala Nagarani’s candidacy for promotion from Lecturer to Senior lecturer, Dept. of Mathematics, University of West Indies, Mona Campus, Jamaica, West Indies
11/2017: Invited External Reviewer of Dr. Rachael Hageman Blair’s candidacy for promotion from Assistant Professor to Associate Professor with Tenure, Dept.
of Biostatistics, State University of New York at Buffalo LOCAL/REGIONAL ELECTED/APPOINTED LEADERSHIP AND COMMITTEE POSITIONS:
Fall 2013: Study Panel Member, CTSI Pilot and Collaborative Clinical and Translational Research Program (Multi-Institutional Study Panel from Greater Milwaukee) 5/2014 – 6/2016: Faculty Experience Committee Member, Joint MU-MCW Department of BME
Initiative; Participated in regular meetings and discussions for the formation of the Joint MU-MCW Department of BME and creation of by-laws and common cultures for the new department
7/2016 – Present: Graduate Committee Member, Department of BME, Marquette University and Medical College of Wisconsin
10/2016 – 4/2017: Co-Developer along with Dr. Said Audi (MU) of the Joint MU-MCW BME PhD Program Handbook and Proposal, which were later (5/2017) approved by the MCW Graduate School of Biomedical Sciences; Joint BME PhD Program was launched on MCW platform effective Fall 2017
12/2016 – 5/2017: Faculty Search Committee Member, Department of BME, Marquette University and Medical College of Wisconsin; resulted in successful recruitment of Dr.
Brandon Tefft at MCW in Tissue Engineering (Chair: Dr. Andy Greene) 1/2017 – Present: Faculty Interview/Recruitment Committee Member, Department of BME,
University of Wisconsin-Milwaukee, WI (January-March, Annually)
7/2017 – 12/2018: Co-Director along with Dr. John LaDisa (MU), Joint MU-MCW BME PhD Program, MCW Graduate School of Biomedical Sciences
Winter 2018: Study Panel Member, Digestive Disease Center Pilot Grant Review Committee 2018, Medical College of Wisconsin; Reviewed 2 pilot grants
1/2019 – Present: Co-Director along with Dr. Said Audi (MU), Joint MU-MCW BME PhD Program, MCW Graduate School of Biomedical Sciences
7/2019 – Present: Promotion and Tenure Committee Member, Department of BME, Marquette University and Medical College of Wisconsin
Spring 2019: Study Panel Member, Cardiovascular Center Pilot Grant Review Committee 2019, Medical College of Wisconsin; Reviewed 5 pilot grants
7/2019 – 9/2019: Faculty Search Committee Member, Department of BME, Marquette University and Medical College of Wisconsin; Resulted in a failed search for an MU faculty position in Biomechanics (Committee Chair: Dr. Brian Schmidt)
3/2020 – 8/2020: Faculty Search Committee Member, Department of BME, Marquette University and Medical College of Wisconsin; Resulted in successful recruitment of Dr. Jim Hokanson for MCW BME faculty position in Biosensors and Implantable Devices (Committee Chair: Dr. Amit Joshi)
1/2021 – Present: Clinical Immersion Certification Program Development Committee Member, Department of BME, Marquette University and Medical College of Wisconsin
MEDICAL COLLEGE OF WISCONSIN COMMITTEES/SERVICES:
6/2006 – 6/2016: Planning Committee Member, Biotechnology and Bioengineering Center (BBC);
Involved in regular meetings and discussions, including retreats, regarding the growth of the Center; Served as a Review Panel Member for BBC Inducement Grant Program for Faculty Collaborations within MCW (Fall 2006); Served as a Review Panel Member for Innovation Center Research Grant Program for Post- doctoral and Clinical Fellows within MCW (Summer 2011)
6/2006 – Present: Recruitment Committee Member; Involved in interviews and recruitments of prospective students for admission into MCW graduate programs (e.g. MSTP, Physiology, BME) and prospective faculties for MCW departments and centers 6/2006 – Present: Miscellaneous MCW services including but not limited to (1) Participating and
contributing to the departmental activities; (2) Promoting the computational biology research program with visiting scientists; (3) Promoting the Institution’s and participating departments’ and centers’ mission; (4) Judging MCW Research Day and graduate/medical students’ poster presentations
9/2011 – 8/2013: Planning Committee Member, Center for Computational Medicine and Virtual Physiological Rat Center; Involved in regular meetings and discussions for the growth of the Centers and computational biology research program
7/2014 – 6/2020: Library Committee Member, MCW Faculty Council; Received Outstanding Faculty Service Award for Service during the year July 2014 – June 2015
7/2015 – 6/2018: Course Evaluation Committee Member, MCW Graduate Studies Council;
Reviewed 4-5 courses each during the spring and summer meetings
7/2016 – Present: Participating Faculty Mentor for prospective undergraduate students under the Diversity Summer Health-Related Research and Education Program (DSHREP),
Program for Undergraduate Research Experience (PURE), and Summer Program for Undergraduate Research (SPUR), Medical College of Wisconsin
7/2017 – Present: Graduate Studies Council Member, Joint MU-MCW BME PhD Program, Graduate School of Biomedical Sciences, Medical College of Wisconsin; Received Dean’s Choice Outstanding Graduate School Educator Award (2016-2017, 2019-2020) 11/2018 – 6/2021: BME Faculty Representative, Department Mentoring Champions Committee of
Faculty Affairs, Medical College of Wisconsin
9/2020 – Present: Admission Committee Member, Medical Student Training Program (MSTP), Medical College of Wisconsin
7/2021 – Present: University Rank and Tenure Committee Member, Medical College of Wisconsin
GRADUATE STUDENT DISSERTATION COMMITTEES/ADVISING:
9/2007 – 7/2010: MS Dissertation Committee Member of Anthony Rick (Advisor: Dr. Daniel Beard), Department of Physiology, Medical College of Wisconsin
9/2007 – 7/2012: PhD Dissertation Committee Member of Mathew Thompson (Advisor: Dr. Daniel Beard), Department of Physiology, Medical College of Wisconsin
11/2013 – 2/2019: PhD Dissertation Co-Advisor of Xiao Zhang (Advisor: Dr. Said Audi), Department of Biomedical Engineering, Marquette University
9/2017 – 5/2018: MS Dissertation Committee Member of Nina Friedly (Advisor: Dr. Said Audi), Department of Biomedical Engineering, Marquette University
9/2017 – 10/2019: MS Dissertation Committee Member of Anthony Cammarata (Advisor: Dr. Said Audi), Department of Biomedical Engineering, Marquette University
9/2017 – Present: PhD Dissertation Director/Advisor of Shima Sadri, Department of Biomedical Engineering, Marquette University and Medical College of Wisconsin 9/2018 – 6/2020: MS Dissertation Director/Advisor of Viren Shah, Department of Biomedical
Engineering, University of Wisconsin – Milwaukee
9/2018 – Present: PhD Dissertation Committee Member of Mir Hadi (Advisor: Dr. Amit Joshi) Department of Biomedical Engineering, Marquette University and Medical College of Wisconsin
9/2019 – 8/2020: PhD Dissertation Committee Member of Choi Hoon (Advisor: Dr. Yoganandan Narayan), Department of Biomedical Engineering, Marquette University and Medical College of Wisconsin
9/2019 – Present: PhD Dissertation Director/Advisor of Justin Womack, Department of Biomedical Engineering, Marquette University and Medical College of Wisconsin
9/2020 – Present: PhD Dissertation Director/Advisor of Viren Shah, Department of Biomedical Engineering, Marquette University and Medical College of Wisconsin
6/2020 – Present: MSTP/PhD Dissertation Co-Advisor of Chris Monti (Advisor: Dr. Scott Terhune), Department of Microbiology and Immunology, Medical College of Wisconsin
9/2020 – 8/2021: MS Dissertation Committee Member of Sweta Ganesh (Advisor: Dr. Said Audi), Department of Biomedical Engineering, Marquette University
9/2020 – Present: PhD Dissertation Committee Member of Patrick Noffke (Advisor: Dr. Janette Strasburger), Department of Biomedical Engineering, Marquette University and Medical College of Wisconsin
RESEARCH GRANTS/AWARDS/CONTRACTS/PROJECTS: Active (Peer Review):
Title: Experimental and Computational Analysis of Mechanisms of Mitochondrial- Cellular ROS Crosstalk in the Kidney in Salt-Sensitive Hypertension
Source: NIH/NHLBI (R01-HL151587)
Role: Principal Investigator/Program Director (Current efforts: 25%) PIs: Allen Cowley (Physiology/MCW); Ranjan Dash
Duration: 1/1/2021 – 12/31/2024
Direct Funds: $1,560,000/4 years ($780,000/4 years for Dash)
Objective: Use a systems biology approach to iteratively conduct experiments and
computationally model the measured data to quantitatively characterize how a high salt diet with associated hypertension produces mitochondrial dysfunction with oxidative stress and reduced efficiency of oxygen utilization/ATP synthesis in the kidney due to mitochondrial-cellular ROS crosstalk.
Title: Predictive Computational Modeling to Define Antiviral Efficacy Source: NIH/NIAID (R21-AI149039)
Role: Principal Investigator (Current efforts: 12%)
PIs: Scott Terhune (Microbiology and Immunology/MCW), Ranjan Dash Duration: 3/18/2020 – 2/28/2022
Direct Funds: $275,000 for 2 years ($137,500 for 2 years for Dash)
Objective: Develop predictive computational models of mitotic cell cycle in conjunction with bench experiments and clinical data to characterize dysregulation of cellular proliferation and differentiation with human cytomegalovirus infection and define the efficacy of antiviral drug treatments.
Past (Peer Review):
Title: Analyzing the Cardiac Power Grid: Adaptive Responses to Fluctuating Local Demand Source: NIH/NHLBI (U01-HL122199)
Role: Principal Investigator of MCW Subcontract
PIs: James Bassingthwaighte (UW Seattle); Daniel Beard (UMich); Ranjan Dash (MCW); Andrew McCulloch (UCSD)
Duration: 9/15/2015 – 5/31/2021 (with NCE)
Direct Funds: $2,150,000 for U01; $240,000 for MCW Subcontract (5 years)
Objective: Use mathematical modeling of cardiac function to construct a framework demonstrating the control of a broad set of biochemical, gene-regulatory, vascular, and tissue networks governing cardiac function.
Title: PPG: Mechanisms of Anesthetic Cardioprotection; Project III: Modeling of Mitochondria and Cell Function in Anesthetic Cardioprotection
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Source: NIH/NIGMS (P01-GM066730) Role: Principal Investigator of Project III
PIs: Zeljko Bosnjak (Project I and Overall); Mingyu Liang (Project II); Ranjan Dash (Project III); Xiaowen Bai (Stem Cell Core)
Duration: 3/1/2015 – 2/29/2021 (with NCE)
Direct Funds: $5,829,380 for PPG; $1,374,435 for Project III (5 years)
Objective: Use a systems biology approach to iteratively conduct experiments and computationally model the measured data to quantitatively characterize the specific effects and molecular mechanisms of action of isoflurane on proteins regulating mitochondrial and cellular functions leading to cardioprotection against ischemia-reperfusion injury, and to determine the mitochondrial and cellular mechanisms that lead to impaired cardioprotection in type-2 diabetes.
Title: SPECT Imaging and Computational Modeling for Assessment of Acute Lung Injury Source: NIH/NHLBI (R15-HL129209)
Role: Co-Investigator (Current efforts: 3%)
PIs: Said Audi (BME/MU); Elizabeth Jacobs (Pulmonary Medicine/MCW) Duration: 1/1/2019 – 12/31/2020
Direct Funds: $300,000 (Salary support for Dash; 3% effort)
Objective: Combine in vivo SPECT imaging and physiologically-based pharmacokinetic (PBPK) modeling of the lung uptake and retention of SPECT biomarkers to mechanistically characterize the effects of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) on specific mitochondrial and cellular targets for developing preclinical therapeutic strategies.
Title: Systems Biology Based Tools for Modeling Platelet Storage Lesion for Optimal Blood Transfusions
Source: US Army (DOD)-CFDRC SBIR Phase II Project (W81XWH-16-C-0106) Role: Principal Investigator of MCW Subcontract
PIs: Andrzej Przekwas and Mahadevabharath Somayaji (CFDRC); Ranjan Dash (MCW); Anand Ramasubramanian (SJSU)
Duration: 9/26/2016 – 1/25/2019
Direct Funds: $58,442 for MCW Subcontract (2 years)
Objective: Develop an integrated systems biology- and physiology-based computational modeling tool to simulate platelet storage lesion and to explore novel methods for extending the shelf-life of platelets stored in bags.
Title: Predictive Computational Modeling to Define Antiviral Efficacy Source: MCW Advancing Healthier Wisconsin Endowment (REP # 5520429) Role: Co-Principal Investigator
PIs: Scott Terhune (Microbiology and Immunology/MCW), Ranjan Dash Duration: 2/1/2017 – 1/31/2019
Direct Funds: $200,000 for 2 years ($100,000 for 2 years for Dash)
Objective: Develop predictive computational models of mitotic cell cycle in conjunction with bench experiments and clinical data to characterize dysregulation of cellular proliferation and differentiation with human cytomegalovirus infection and define the efficacy of antiviral drug treatments.
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(6) Title: Mechanistic Characterization of Calcium and ROS Induced Mitochondrial Dysfunction
Source: NIH/NHLBI (K99/R00-HL121160)
Role: Co-Mentor/Co-Advisor (no salary support)
PIs: Jason Bazil (Assistant Professor, Physiology, MSU-East Lansing) Duration: 9/16/2014 – 1/31/2019
Direct Funds: $714,511 for 5 years
Objective: Develop an integrated experimental and computational research program to mechanistically characterize calcium and reactive oxygen species induced mitochondrial dysfunction in disease pathologies.
(7) Title: Cations and ROS Modulating Mitochondrial Function in Normal and Ischemic Hearts Source: NIH/NHLBI (R01-HL095122)
Role: Principal Investigator/Program Director
PIs: Ranjan Dash (Physiology/MCW); Amadou Camara (Anesthesiology/MCW) Duration: 4/1/2010 – 3/31/2015 (with 1 year NCE)
Direct Funds: $1,400,000/4 years ($700,000/4 years for Dash)
Objective: Obtain dynamic data on mitochondrial cations, ROS, and bioenergetics, and use the obtained data to develop and validate dynamic computational models to quantitatively characterize biophysical and biochemical mechanisms associated with mitochondrial cations and ROS handlings in the heart in dynamic regulation of mitochondrial function under normal and ischemic conditions.
(8) Title: Virtual Physiological Rat Project Source: NIH/NIGMS (P50-GM094503)
Role: Co-Investigator and Project Leader of Project II (8/11/11 – 8/31/13) PI: Daniel Beard (MCW; moved to UMich in 9/2013)
Duration: 8/11/2011 – 6/30/2016
Direct Funds: $13,000,000 (Salary support for Dash based on efforts)
Objective: Establish a NIH/NIGMS National Center of Systems Biology to develop and apply a systems biology research platform for studying physiological mechanisms of complex cardiovascular diseases through mechanistic multi-scale computational models and engineered rat models of cardiovascular disease.
(9) Title: Integrated Modeling of Cardiac Metabolism and Transport Source: NIH/NHLBI (R01-HL072011)
Role: Co-Investigator (6/1/2006 – 8/31/2013)
PI: Daniel Beard (MCW; moved to UMich in 9/2013)
Direct Funds: $250,000/year (Salary support for Dash based on efforts) Duration: 12/1/2002 – 5/31/2017
Objective: Develop computational models of cardiac tissue oxygen transport and cellular energy metabolism and apply the developed models to analyze experimental data on myocardial oxygenation and oxidative energy metabolism under ischemic and exercise conditions.
(10) Title: Mechanisms of Metabolic Dysfunction in Type-2 Diabetes Source: NIH/NIDDK (R01-DK095210)
Role: Co-Investigator (4/1/2012 – 8/31/2013)
PIs: Daniel Beard (MCW; moved to UMich in 9/2013); Robert Wiseman (MSU) Duration: 4/1/2012 – 3/31/2017
Direct Funds: $420,000/year (Salary support for Dash based on efforts)
Objective: Obtain kinetic data on mitochondrial metabolic pathways and 31PNMR data on cellular phosphoenergetics from normal and diseased (diabetic) rat skeletal muscle and use the obtained data to identify computational models of skeletal muscle cellular energy metabolism, and then use the developed models to determine how the control of energy metabolism fails and remodeling occurs in skeletal muscle in diabetes.
(11) Title: PPG: Anesthetic-Induced Cardiac Preconditioning (Project II: Mitochondrial Function in Anesthetic-Induced Cardiac Preconditioning)
Source: NIH/NIGMS (P01-GM066730)
Role: Co-Investigator (8/1/2008 – 7/31/2013) PI: Zeljko Bosnjak (Anesthesiology/MCW) Duration: 8/1/2008 – 7/31/2013
Direct Funds: $1,250,000/year (Salary support for Dash based on efforts)
Objective: Computational modeling specific aim of Project II was to investigate the direct effects of volatile anesthetics (isoflurane) on cardiac mitochondrial function that leads to anesthetic preconditioning (APC).
(12) Title: Mechanisms of Metabolic Dysfunction in Heart Disease Source: NIH/NHLBI (R01-HL094317)
Role: Co-Investigator
PI: Daniel Beard (BBC and Physiology/MCW) Duration: 1/1/2009 – 12/31/2012
Direct Funds: $225,000/year (Salary support for Dash based on efforts)
Objective: Obtain kinetic data on mitochondrial pathways from normal and diseased (heart failure) rats and use the obtained kinetic data to identify computational models of mitochondrial carbohydrate and fatty acid metabolism, and then use the developed models to determine how molecular-level changes in mitochondrial enzymes and transporters that occur in heart disease affect overall cardiac function and how therapies may be targeted at the molecular level to improve function at the whole-organ level.
(13) Title: Inflammation and Infection in Acquired and Congenital Cardiovascular Disease Source: NIH/NHLBI (T32-HL094273)
Role: Participating Faculty Mentor (Mentored Jason Bazil; 9/2010 – 8/2013) PI: Andrew Greene (BBC and Physiology/MCW)
Duration: 5/1/2009 – 4/30/2014
Direct Funds: $100,000/year (No salary support for faculties)
Objective: Develop a highly interdisciplinary, translational postdoctoral training program focused on inflammation and infection in the development and progression of cardiovascular disease.
(14) Title: Database-Enabled Tools for Regulatory Metabolic Networks Source: NSF Biological Database and Informatics (DBI-0743705) Role: Co-Principal Investigator (PI of MCW Subaward)
PI: Meral Ozsoyoglu (Case Western Reserve University) Duration: 8/1/2009 – 7/31/2012
Direct Funds: $51,589 for MCW Subaward for 3 years
Objective: Design and implement a database-enabled framework and tools to facilitate effective and efficient model development for multi-scale mechanistic models of biological systems.
(15) Title: Computational Modeling of Mitochondrial Energetics Source: AHA National (SDG-0735093N)
Role: Principal Investigator/Program Director
PI: Ranjan Dash
Duration: 7/1/2007 – 6/30/2011
Direct Funds: $65,000/year ($260,000 for 4 years)
Objective: Develop mathematical models of mitochondrial cation transporters (e.g. Na+- Ca2+ cycling) based on available kinetic data from literature on the transporter function, and integrate the cation transporter models into existing models of mitochondrial oxidative phosphorylation, TCA cycle, and electrophysiology to simulate and analyze the effects of cations on mitochondrial bioenergetics and ROS emission (generation – scavenging).
(16) Title: Time Course of Metabolic Adaptations during Loading and Unloading Source: NASA Johnson Space Center (NNJ06HD81G)
Role: Co-Principal Investigator (PI of MCW Subaward) PI: Marco Cabrera (Case Western Reserve University) Duration: 6/12/2006 – 6/11/2009
Direct Funds: $35,280 for MCW Subaward for 2 years
Objective: Develop multi-scale computational models of skeletal muscle metabolism and simulate time course of metabolic adaptations to actual and simulated microgravity (unloading) and chronic endurance training (loading).
(17) Title: Multi-Scale Modeling of the Heart in Cardiovascular Disease and Metabolic Syndrome
Source: NIH/NIBIB (R01-EB005825)
Role: Co-Investigator (6/1/2006 – 7/31/2008) PI: Daniel Beard (BBC-Physiology/MCW) Duration: 8/1/2005 – 7/31/2008
Direct Funds: $250,000/year (Salary support for Dash based on efforts)
Objective: Integrate computational models of the heart, including cellular and whole-organ electrophysiological simulations with metabolic and mechanical simulations, to understand how these systems interact in the heart in health and disease.
(18) Title: Analysis of Large-Scale Biochemical Systems Source: NIH/NIGMS (R01-GM068610)
Role: Co-Investigator (6/1/2006 – 7/31/2008) PI: Daniel Beard (BBC-Physiology/MCW) Duration: 8/1/2004 – 7/31/2008
Direct Funds: $250,000/year (Salary support for Dash based on efforts)
Objective: Development of software for analyzing and simulating systems of biochemical
reactions and coupling of whole-body transport and cellular reaction systems for physiologically-based pharmacokinetic modeling, and application of these tools to drug delivery and metabolism.
(19) Title: Center for Modeling Integrated Metabolic Systems Source: NIH/NIGMS (P50-GM066309)
Role: Co-Investigator (9/2003 – 5/2006)
PI: Gerald Saidel (Case Western Reserve University) Duration: 7/1/2002 – 6/30/2007
Direct Funds: $2,000,000/year (100% Salary support for Dash)
Objective: Establish an NIH/NIGMS National Center of Systems Biology that combines mathematical modeling, computer simulation, and in vivo experimentation to quantify relationships between cellular metabolism and physiological responses of tissue-organ systems and the whole body to various perturbations (e.g. ischemia, hypoxia, exercise).
MEDICAL COLLEGE OF WISCONSIN TEACHING ACTIVITIES:
Fall 2007: Developed and taught (Course Co-Director) along with Dr. Daniel Beard a part of a three-credit systems biology course (50% of the course, spanning 14 weeks, totaling 28 1.5-hrs lectures) “PHYS8275, Special Topics in Physiology: Chemical Biophysics of Cellular Processes” to the graduate students at the Medical College of Wisconsin. This course was aimed at developing mathematical models and computational tools for the simulation and analysis of integrated biochemical systems.
Fall 2009: Developed and taught (Course Co-Director) along with Dr. Daniel Beard a part of a three-credit systems biology course (50% of the course, spanning 14 weeks, totaling twenty-eight 1.5-hr lectures) “PHYS8284, Special Topics in Physiology: Computational Methods in Biomedical Research” to the graduate students at the Medical College of Wisconsin. This course was focused on practical techniques (computational models and methods) for the simulation and analysis of biological/physiological systems, developed largely via application-driven examples. In teaching, the important details of the underlying biological systems were described along with a complete step-by- step development of model assumptions, resulting equations, and (when necessary) computer codes.
Fall 2011: Developed and taught (Course Co-Director) along with Drs. Daniel Beard and Brian Carlson a part of an one-credit course (33% of the course, spanning 14 weeks, totaling fourteen 1-hr sessions) “PHYS8275, Special Topics in Physiology: Classic Papers in Physiology Research” to the physiology graduate students at the Medical College of Wisconsin. The goal of this course was to study classic works from the physiology literature, signifying quantitative methodology, critical thinking, scientific rigor, and major mechanistic insights. In the course of the 10-week period of the course, two students per week were asked for a directed presentation of one of the classic papers.
Spring 2016: Taught one 1.5-hr lecture as a Guest Lecturer on “Multi-Scale and Systems Modeling of Cardiac Energy Metabolism and Ca2+-ROS Handling in Health and Disease” as a part of graduate course “BIEN5720, Cardiopulmonary Mechanics” (Instructor: Dr. John LaDisa) for Biomedical Engineering graduate students at Marquette University. Prepared lecture
notes and PowerPoint slides and shared with the participating students.
Spring 2016,
Spring 2017 Taught two 2-hr lectures as a Guest Lecturer on “Introduction to Computational Modeling of Biological/Physiological Systems” as a part of a multi-disciplinary graduate course “PHYS8230, Physiological Genomics” (Course Director: Dr. Mingyu Liang) for the physiology graduate students at the Medical College of Wisconsin. Prepared lecture notes and PowerPoint slides and shared with the participating students.
Spring 2018, Spring 2021
Developed and taught (Course Co-Director) along with Dr. Said Audi a part of a three- credit systems biology course (>50% of the course, spanning 14 weeks, totaling twenty- eight 1.5-hr lectures) “BIEN6391, Special Topics in BME: Modeling and Simulations of Integrated Cellular Systems” to the BME graduate students at the Medical College of Wisconsin and Marquette University. This course was focused on the use of kinetic and thermodynamic principles for modeling and analysis of integrated cellular systems.
Examples of systems at different levels of biological complexity was covered, starting with physiology and biochemistry of the system, use of basic physiochemical laws to derive the governing differential equations, and use of numerical methods to simulate the systems behavior under normal and pathological conditions using computer programming (MatLab).
Fall 2019 Taught two 1.5-hr lectures as a Guest Lecturer on “Introduction to Modeling and Simulations of Integrated Biological Systems” as a part of a BME graduate course
“BIEN6710, Special Topics in BME: Cellular and Molecular Bioengineering” (Course Director: Dr. Brian Hoffmann) for the BME graduate students at the Medical College of Wisconsin and Marquette University. Prepared lecture notes and PowerPoint slides and shared with the participating students. Also took a test on the topic and evaluated.
EXTRAMURAL AND PAST TEACHING ACTIVITIES: 1/1993
– 7/1998: Involved in classroom teaching and tutoring Calculus I and II, Complex Analysis, Numerical Analysis, Ordinary Differential Equations, Initial and Boundary Value Problems, and Applied Mathematical and Computational Methods to the under- graduate engineering students at the Indian Institute of Technology, New Delhi, as a teaching assistant; Additional teaching load included test preparation and grading test papers, homework, quizzes, and computer lab assignments.
Winter 2001, Winter 2002:
Taught part of a bioengineering graduate course BIOEN589 (Bioengineering Principles of Physiology) floated by Dr. James Bassingthwaighte at the University of Washington; Developed two computational teaching models for the course, which were used in laboratory classes for computational simulations; These were (1) a model for computing oxygen and carbon dioxide saturations of hemoglobin under varying physiological conditions and for computing oxygen and carbon dioxide contents in the whole blood, and (2) a four-compartmental recirculating model for transport and exchange of oxygen, carbon dioxide, bicarbonate, and hydrogen ions between the lungs and systemic circulation.
MCWSTUDENTS,POSTDOCTORAL FELLOWS, AND FACULTY MENTORED: 6/2006
– Present:
Working closely with graduate students and postdoctoral fellows associated with computational biology research. Providing general consultations on specific aspects of
mathematical modeling and computer simulations of transport and reaction processes in complex metabolic systems. Involved in educating, supervising, and mentoring of undergraduate and high school students interested in computational biology research.
Short-Term Students Supervised/Mentored (SPUR, MSTP, High School):
Summer 2006:
Supervised Victoria Georgakas and Aleksandra Polosukhina, 2006 MCW SPUR students in our Computational Biology Laboratory, on computational modeling of mitochondrial energy metabolism and Na+-Ca2+ cycling.
July 2012: Supervised David Schauder, a MSTP MD/PhD student in MCW, who completed a one- month rotation in our Computational Biology Laboratory, learning combined
experimental-computational approach to characterize mitochondrial Ca2+ buffering system.
July 2013: Supervised Amin Bemanian, a MSTP MD/PhD student in MCW, who completed a one- month rotation in our Computational Biology Laboratory, developing computational models of cell cycle (proliferation and differentiation) dysregulation with viral infection.
Fall 2016 – Summer 2018:
Mentored Nabeel Quryshi, a high school student (junior-senior), who was highly
interested in learning about computational biology research. Nabeel learned quite a bit over two years and received several first-place awards at different fairs and
competitions (e.g., Intel International Science and Engineering Fair, May-2017). He joined Harvard University in Fall 2018 for his dual undergraduate and graduate studies (for majors in premedical sciences and master of public health).
Summer 2017:
Supervised Brittany Sheahan, 2017 MCW SPUR student in our Computational Biology Laboratory, on the computational modeling of mitochondrial energy metabolism and K+ dynamics in calibrating membrane potential from fluorescent measurements.
Supervised Jeannette Ingabire, 2018 MCW SPUR student in my Computational Biology Laboratory, on the computational modeling of cardiac L-type Ca2+ channel function and regulation through various mechanisms (e.g., voltage and Ca2+).
Supervised Janelle Lanham, 2019 MCW SPUR student in my Computational Biology Laboratory, on the computational modeling of mitochondrial energy metabolism in vascular endothelial cells in type 2 diabetes and hyperglycemia.
Supervised Grace Gibson, 2021 MCW SPUR student in my Computational Biology Laboratory, on the computational modeling of mitochondrial Ca2+ homeostasis and regulation of energy metabolism in the heart and kidney.
Graduate Students Supervised/Mentored:
MS Thesis Committee Member and Co-Mentor of Anthony Rick who worked in our Computational Biology Laboratory (Mentor: Daniel Beard) and graduated with MS Degree in Physiology with thesis titled “Identification of Catalytic Mechanism of Mitochondrial Malate Dehydrogenase”.
PhD Thesis Committee Member and Co-Mentor of Matthew Thompson who worked in our Computational Biology Laboratory (Mentor: Daniel Beard) and graduated with a PhD in Physiology with thesis titled “Physiologically-Based Pharmacokinetic
8/2007 – 7/2010:
8/2007 – 7/2012:
Summer 2018:
Summer 2019:
Summer 2021:
Modeling of Drug Transport and Metabolism”.
PhD Thesis Committee Member and Co-Mentor of Xiao Zhang in the Department of Biomedical Engineering at Marquette University (Mentor: Said Audi), who worked on multi-scale computational modeling of lung tissue bioenergetics to quantitatively characterize cellular and mitochondrial targets in acute lung injury (ALI) and acquired respiratory distress syndrome (ARDS).
PhD Thesis Committee Director and Advisor of Shima Sadri in the Joint Department of Biomedical Engineering, Medical College of Wisconsin and Marquette University. She is working on computational modeling of mitochondrial bioenergetics and ROS homeostasis to quantitatively characterize underlying kinetic mechanisms of mitochondrial dysfunction and ROS dysregulation in the kidney in salt-sensitive hypertension.
MS Thesis Committee Director and Advisor of Viren Shah in the Department of Biomedical Engineering, University of Wisconsin-Milwaukee. He worked on computational modeling of mitochondrial bioenergetics with altered VDAC kinetics for ions and metabolites.
PhD Thesis Committee Director and Advisor of Justin Womack in the Joint Department of Biomedical Engineering, Medical College of Wisconsin and Marquette University, who is interested in problems of computational virology (cell cycle dysregulation with viral infection) (Co-Advisor: Dr. Scott Terhune).
MSTP/PhD Thesis Committee Member and Co-Advisor of Chris Monti in the Department of Microbiology and Immunology, Medical College of Wisconsin, who is interested in problems of computational virology (viral DNA replication and viral class protein synthesis) (Advisor: Dr. Scott Terhune).
PhD Thesis Committee Director and Advisor of Viren Shah in the Joint Department of Biomedical Engineering, Medical College of Wisconsin and Marquette University, who is interested in problems of computational immunology (Co-Advisor: Dr. Terhune).
Postdoctoral Fellows Supervised/Mentored:
Partially supervised Johan Haumann, who was a postdoctoral fellow with Dr. David Stowe (Anesthesiology), in designing specific experiments to investigate the dynamic regulations of mitochondrial free [Ca2+] during different states of respiration.
Partially sponsored Kalyan Vinnakota first as a postdoctoral fellow with Dr. Daniel Beard and then as a Research Scientist in our Computational Biology Laboratory through my AHA scientific development grant and my NIH R01 grant on using sensitivity analysis to design specific experiments and identify biochemical/metabolic systems. Kalyan then (8/2013) moved as a Research Assistant Professor to the Department of Molecular and Integrative Physiology, University of Michigan. He is currently a Program Manager in Gilbert Family Foundation, Detroit, MI.
Supervised and sponsored Ranjan Pradhan for postdoctoral researcher first through my AHA scientific development grant and then through my NIH R01 grant to computationally model and mechanistically characterize the kinetics of mitochondrial cation transport system (Na+-Ca2+ cycle) in cardiomyocytes and to understand the crucial roles of cations (Ca2+) in the dynamic regulations of mitochondrial function under normal and ischemic conditions. Ranjan then (8/2013) moved as a Research Scientist to the Department of 12/2013
– 3/2019:
9/2018 – 6/2020:
7/2007 – 6/2009:
1/2008 – 8/2013:
8/2008 – 8/2013:
9/2019 – Present:
9/2017 – Present:
7/2020 – Present:
9/2020 – Present:
6/2015
Molecular and Integrative Physiology, University of Michigan. He is current an Assistant Professor in Kalinga Institute of Technology, Bhubaneswar, Orissa, India.
Partially supervised Age Boelens, who was a postdoctoral fellow (Anesthesiology) working on Drs. David Stowe’s and Amadou Camara’s AHA and NIH grants, in designing specific experiments to study the effects of extra-matrix [Mg2+] on mitochondrial Ca2+
uptake and intra-matrix free [Ca2+] regulations during different states of respiration.
Supervised and sponsored Bhawana Agarwal for postdoctoral research, who worked on the project “Mitochondrial Function in Anesthetic Preconditioning”, as a part of Dr.
Bosnjak’s PPG “Anesthetic-induced Cardiac Preconditioning”. Specifically, I supervised her in the designing of crucial experiments to investigate the direct effects of volatile anesthetics (isoflurane) on mitochondrial function (bioenergetics, electrophysiology, cation homeostasis, and ROS emission) that leads to cardioprotection. Bhawana is currently a clinical coordinator in a pharmaceutical company.
Supervised and sponsored Jason Bazil for postdoctoral research through BBC’s T32 grant and my NIH R01 grant to develop computational models of mitochondrial bioenergetics and cation and ROS handlings, and to utilize the model-driven experimental designs to characterize the kinetics of mitochondrial cation transport and sequestration and ROS generation and scavenging systems in cardiomyocytes to quantitatively characterize the crucial roles of cations (e.g., Ca2+) and ROS in the dynamic regulations of mitochondrial function under normal and ischemia-reperfusion conditions. Jason then (8/2013) moved as a Postdoctoral Fellow to the Department of Molecular and Integrative Physiology, University of Michigan. Based on the research in my laboratory, Jason was successful in receiving a K99/R00 grant. Jason is currently an Assistant Professor in the Department of Physiology in the Michigan State University.
Partially supervised Christoph Blomeyer, who was a postdoctoral fellow (Anesthesiology) working on Dash-Camara’s R01 project, in designing specific experiments to investigate the effects of extra-matrix [Na+] on mitochondrial Ca2+ efflux and intra-matrix free [Ca2+] regulations during different states of respiration.
Supervised and sponsored Shivendra Tewari for postdoctoral research through my NIH R01 grant to develop computational models of mitochondrial bioenergetics and cation handling, and to use the model-driven experimental designs to characterize the kinetics of mitochondrial cation transport and buffering (sequestration) in cardiomyocytes and to understand the crucial roles of cations (e.g., Ca2+) in the dynamic regulations of mito- chondrial function under normal and ischemic conditions. Shivendra then (11/2013) moved as a Postdoctoral Fellow to the Department of Molecular and Integrative Phy- siology, University of Michigan. Shivendra is currently a Research Scientist II in the US Army Systems Biology Division, Fredrick, MD.
Supervised and sponsored Venkat Pannala for postdoctoral research through my NIH R01 grant to computationally characterize the kinetics of mitochondrial bioenergetics and ROS generation and scavenging in cardiomyocytes to quantitatively understand the crucial roles of ROS in the dynamic regulation of mitochondrial function under normal and ischemic-reperfusion conditions. Venkat is currently a Research Scientist II in the US Army Systems Biology Division, Fredrick, MD.
Supervised and sponsored Neeraj Manhas for postdoctoral research through my NIH 11/2010
– 9/2013:
2/2012 – 11/2013:
7/2012 – 4/2016:
3/2009 – 4/2015:
3/2009 – 2/2011:
9/2010 – 8/2013:
grant (PPG Project III), who worked on mathematical modeling (spatial-temporal) of cardiomyocytes electrophysiology and Ca2+ dynamics, incorporating SR-mitochondria microdomain and crosstalk in signal transduction and energy homeostasis, to study mechanisms of cardioprotective effects of volatile anesthetics (isoflurane). Neeraj is currently an Assistant Professor in an Engineering College in India.
Supervised and sponsored Yongwoon Jung for postdoctoral research through my AHW collaborative grant with Dr. Scott Terhune, who worked on developing computational models of cell cycle (proliferation and differentiation) dysregulation with human cytomegalovirus (HCMV) infection to identify key biomarkers for antiviral treatments as well as to define the efficacy of antiviral treatments.
Supervising and sponsoring Namrata Tomar for postdoctoral research through my NIH grants, who is experimentally characterizing the effects of respiratory substrates, Ca2+, and ADP on bioenergetics (respiration, NADH redox state, and membrane potential) and Ca2+-ROS handlings in the heart and kidney mitochondria under different conditions (e.g., low and high Ca2+, ischemia-reperfusion injury, and salt-sensitive hypertension).
Supervised and sponsored Sunil Kandel for postdoctoral research through my NIH grants, who worked on experimentally characterizing the effects of respiratory substrates, Ca2+, and ADP on bioenergetics (respiration, NADH redox state, and membrane potential) and Ca2+-ROS handlings in the heart and kidney mitochondria under different conditions (e.g., low and high Ca2+, ischemia-reperfusion injury, and salt-sensitive hypertension).
Supervising and sponsoring Xiao Zhang for postdoctoral research through my NIH grants for developing computational models of mitochondrial bioenergetics and cations/ROS handlings to mechanistically characterize the kinetics of mitochondrial cation transport and buffering (sequestration) and ROS generation and scavenging in cells, as well as to understand the crucial roles of cations (e.g., Ca2+) and ROS in the dynamic regulations of mitochondrial function in the heart and kidney (cortex and medulla) under normal and pathological conditions, such as slat-sensitive hypertension.
EXTRAMURAL STUDENTS,POSTDOCTORAL FELLOWS, AND FACULTY MENTORED:
Supervised the MIMS Center’s 2004 summer internship undergraduate students Krisanne Litinas on the “Modeling of Oxygen Transport and Utilization in Skeletal Muscle at Onset of Exercise” and Jessica Ferrato on the “Constraint-based Analysis of Cellular Metabolic Pathways in Skeletal Muscle” at Case Western Reserve University, Cleveland.
Supervised the MIMS Center’s 2005 summer internship undergraduate student Eric Xu working on the analysis of pulmonary oxygen uptake (VO2) data collected in our human exercise laboratory to understand the “on” and “off” kinetics of VO2 associated with exercise onset and recovery of various intensities (moderate, heavy and very heavy) at Case Western Reserve University, Cleveland.
Supervised and worked closely with the MIMS Center’s graduate students at Case Western Reserve University, Cleveland. Provided general consultations on specific aspects of mathematical modeling and computer simulations of transport and metabolic processes in complex physiological/metabolic systems:
■ Jaeyeon Kim on the topic “Regulation of Whole-Body Energy Metabolism and 6/2004
– 8/2004:
9/2003 – 5/2006:
6/2005 – 8/2005:
12/2016 – 6/2019:
9/2017 – 9/2019:
4/2019 – Present:
4/2016 – Present:
Fuel Homeostasis during Exercise”.
■ Yanjun Li on the topic “Regulation of Cellular Metabolism and Energetics in Skeletal Muscle during Ischemia and Exercise”.
■ Haiying Zhou on the topic “Whole-Body Gas Exchange and Acid-Base Balance during Hypoxia and Exercise”.
Regularly advising Ben Korman, BSc, MBBS, MD, FFARACS, FANZCA, who is an
anesthesiologist at Royal Perth Hospital, and currently conducting doctoral dissertation work at the University of Western Australia, Perth. Ben is working on mathematical modeling of gas exchange in the lungs involving a mixture of physiological and inert gases to study the effects of ventilation-perfusion inequality on gas exchange, second gas effects, and lungs function. I am regularly advising him via emails and skype on mathematical modeling and numerical computing aspects of the problem.
INVITED/PEER-REVIEWED LECTURES/SYMPOSIUMS/WORKSHOPS/PRESENTATIONS:
1) “Estimation of increased wall shear stress during coronary angioplasty – a theoretical model”.
23rd National Conference on Fluid Mechanics and Fluid Power, Bhopal, India, Dec 27-29, 1996.
[PEER-REVIEWED] [INTERNATIONAL]
2) “A new class of models and their study for bi-directional propagation of capillary-gravity water waves”. 36th Annual Meeting of the Society of Engineering Science, University of Texas, Austin, Oct 25-27, 1999. [PEER-REVIEWED] [NATIONAL]
3) “A new class of model equations for two-way propagation of capillary-gravity water waves”. 52nd Annual Meeting of the American Physical Society, Division of Fluid Dynamics, New Orleans, Nov 21- 23, 1999. [PEER-REVIEWED] [NATIONAL]
4) “A numerical study of pulsatile blood flow in an eccentric catheterized artery using a fast algorithm”.
Department of Mathematics Seminar, Indian Institute of Technology, Kharagpur, India, Aug 16, 2002.
[INVITED] [INTERNATIONAL]
5) “A numerical study of blood flow in a catheterized curved artery with stenosis”, National Simulation Resource for Circulatory Mass Transport and Exchange Seminar, Department of Bioengineering, University of Washington, Seattle, WA, Oct 2, 2000. [INVITED] [REGIONAL/LOCAL]
6) “Computational modeling of oxygen and carbon dioxide transport and exchange in the physiological systems”, Resource Facility for Population Kinetics Seminar, Department of Bioengineering, University of Washington, Seattle, WA, Sept 25, 2002. [INVITED] [REGIONAL/LOCAL]
7) “Computational modeling of oxygen and carbon dioxide transport and exchange in the physiological systems”, Department of Applied Science, College of William & Marry, Williamsburg, VA, Oct 24, 2002. [INVITED] [NATIONAL]
8) “Estimation of muscle oxygen uptake during muscle contraction and recovery using deconvolution”.
Special Session on Microvascular Oxygen Exchange, Biomedical Engineering Society Annual Meeting, Nashville, Oct 1-4, 2003. [INVITED/PEER-REVIEWED] [NATIONAL]
9) “Application of constraint-based estimation methods to complex metabolic systems”, MIMS Center Summer Workshop on Modeling Metabolic Dynamics, Case Western Reserve University, Cleveland, OH, June 11, 2004. [INVITED] [REGIONAL/LOCAL]
9/2015 – Present:
10) “Application of large-scale computational methods to complex metabolic system models”, MIMS Center Advisory Board Meeting, Case Western Reserve University, Cleveland, OH, Oct 22, 2004.
[INVITED] [REGIONAL/LOCAL]
11) “A model of oxidative phosphorylation in mammalian skeletal muscle (Korzeniewski and Zoladz, Biophys Chem 92:17-34, 2001)”, MIMS Center Journal Club, Case Western Reserve University, Cleveland, OH, Feb 25, 2005. [INVITED] [REGIONAL/LOCAL]
12) “Control of respiration and bioenergetics during muscle contraction (Chung et al, Am J Physiol Cell Physiol 288:730-738, 2005)”, MIMS Center Journal Club, Case Western Reserve University, Cleveland, OH, Apr 29, 2005. [INVITED] [REGIONAL/LOCAL]
13) “Skeletal muscle energy metabolism: modeling and experimental validation”, MIMS Center Review, Case Western Reserve University, Cleveland, OH, July 22, 2005. [INVITED] [REGIONAL/LOCAL]
14) “Cellular metabolic dynamics in skeletal muscle during ischemia – In silico studies”, Biotechnology and Bioengineering Center Journal Club, Medical College of Wisconsin, Milwaukee, WI, June 28, 2006. [INVITED] [REGIONAL/LOCAL]
15) “Cellular metabolic dynamics in skeletal muscle during ischemia – In silico studies using a multi-scale top-down integrated model”, Fifth World Congress of Nonlinear Analysis (WCNA 2008), Orlando, FL, July 2-9, 2008. [INVITED/PEER-REVIEWED] [NATIONAL]
16) “Modeling regulation of mitochondrial free Ca2+ by ATP/ADP-dependent Ca2+ buffering”. Special Session on Oxidative Phosphorylation and Mitochondrial Metabolism, Biophysical Society Annual Meeting, Boston, Feb 28 – Mar 4, 2009. [INVITED/PEER-REVIEWED] [NATIONAL]
17) “Mechanism-oriented computational modeling of mitochondrial bioenergetics and cation handling”.
Department of Physiology Seminar, Medical College of Wisconsin, Milwaukee, WI, Oct 13, 2009.
[INVITED] [REGIONAL/LOCAL]
18) “Towards reevaluating mitochondrial Ca2+ dynamics”. Mentor-Mentee Seminar (RK Dash and JN Bazil), Biotechnology Bioengineering Center, Medical College of Wisconsin, Milwaukee, WI, May 3, 2011. [INVITED] [REGIONAL/LOCAL]
19) “Roles of cations and ROS in the dynamic regulation of mitochondrial function in normal and ischemic hearts”. Faculty Spotlight Presentation, Biotechnology Bioengineering Center, Medical College of Wisconsin, Milwaukee, WI, Sep 20, 2011. [INVITED] [REGIONAL/LOCAL]
20) “Regulation of pyruvate dehydrogenase in skeletal muscle during altered energy demand: Inference from computational analysis of phosphoenergetics data from 31P-NMRS”. National Centers for Systems Biology Annual Meeting, Chicago, July 17-18, 2012. [NATIONAL]; VPR Project Advisory Board Meeting, Milwaukee, July 26-27, 2012. [REGIONAL/LOCAL] [TWO INVITED POSTER PRESENTATIONS]
21) “Computational analyses of mitochondrial and cellular function in anesthetic-induced cardiac preconditioning”. Department of Anesthesiology PPG Project III Presentation, Medical College of Wisconsin, Milwaukee, November 28, 2012. [INVITED] [REGIONAL/LOCAL]
22) “Regulation of mitochondrial energy metabolism in cardiac and skeletal muscle during altered energy expenditure: role of pyruvate dehydrogenase”. Department of Physiology Seminar, Medical College of Wisconsin, Milwaukee, WI, Mar 6, 2013. [INVITED] [REGIONAL/LOCAL]
23) “Mechanisms of the mitochondrial glutathione ROS scavenging systems”. Mentor-Mentee Seminar
