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(2) ´ µ¶ @·¸ A¹ºK* B» C** D¼½ JK LMNL OLP * LQRSLTUOLP ** VGWXYZ[\]^_PL`Ga. An Novel Enzyme-linked Immunosorbent Assay for Barley Lipid Transfer Protein as a Quality Control System of Beer Yukie Murakami-Yamaguchi, Junko Hirose*, Wataru Fujii** and Hiroshi Narita Department of Food and Nutrition, Kyoto Women’s University, -/ Kitahiyoshi-cho, Imakumano, Higashiyama-ku, Kyoto 0*/ῌ2/*+ * Department of Food Science and Nutrition, School of Human Cultures, The University of Shiga Prefecture, ,/** Hassaka-cho, Hikone, Shiga /,,ῌ2/-** Safety Science Institute, Quality Assurance Division, Suntory Limited, +ῌ+ῌ+ Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka 0+2ῌ2/*῍῍῍῍῍῍῍῍῍῍῍῍῍῍῍῍ Using a polyclonal antibody raised against lipid transfer protein (LTP) purified from barley flour, a new sandwich-type enzyme-linked immunosorbent assay method was developed to detect barley LTP at concentrations of *.+ῌ+* ng/mL. This method showed *.**-ῌ cross reactivity against LTP of wheat and no cross reactivity against that of oats, adlay, and rye. LTP was detected in several foods containing barley, including fermented foods such as vinegar, soybean paste and beer. The LTP content was approximately the same for similar kinds of beer, even for those manufactured at di#erent facilities or on di#erent production days. Controlling and monitoring the LTP content in beer is important, as LTP is not only known as a factor of foam formation and stability, but also as an allergen. Therefore, this detection method of the LTP content may be a useful tool for quality control of beer. (Received Aug. ,1, ,**2 ; Accepted Oct. ,+, ,**2) Keywords : beer, ELISA, food allergy, lipid transfer protein. ῍ῌ῏ῌ῎ : lipid transfer protein, ELISA,
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(91) OR. 35)D LTP S "TUK #$5. +-. LTP ";<= V WX8Y Z'[\ )N]1^. _`)N]Ka ;<=bc=d1 )ef135)N ] .5) g.;<= "hi1j]k. +.. l Bmno pqg1 )rstu#I]vwZ.xy `. +/. z{ |'}~ )` A ABKz{ y0 y0 y0'%N 6j ῍ + ,. -. . /. 0. 1. 2. 3. +*. ++. ῌ. Kader, J.C., Lipid-transfer proteins in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol., .1, 0,1ῌ0/. (+330). Douliez, J.P., Michon, T., Elmorjani, K. and Marion, D., Structure, biological and technological functions of lipid transfer protein and indolines, the major lipid binding protein from cereal kernels. J. Cereal Sci., -,, +ῌ ,* (,***). Garcia-Olmedo, F., Molina, A., Segura, A. and Moreno, M., The defensive role of nonspecific lipid-transfer proteins in plants. Trends Microbiol., -, 1,ῌ1. (+33/). Kader, J.C., Lipid-transfer proteins : a puzzling family of plant proteins. Trends Plant Sci. Biol., ,, 00ῌ1* (+331). 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(92) 15 . ,0. ,1. ,2. ,3. >?ῌ@ : A#%;BCDEFGHIJ. caria from beer : an immediate hypersensitivity reaction due to a +* kDa protein derived from barley. Clin. Exp. Allergy, ,3, .*1ῌ.+- (+333). Garcia-Casado, G., Crespo, J.F., Rodriguez, J. and Salcedo, G., Isolation and charasterization of barley lipid transfer protein and protein Z as beer allergens. J. Allergy Clin. Immunol., +*2, 0.1ῌ0.3 (,**+).
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As such, this study investigated the effect of sample size on the efficiency of six commonly used count data models namely: Poisson regression model (PRM), Negative binomial
The sliding track is almost totally covered with brass film and this layer protects the surface from the hard asperities of the steel disc continued sliding results
