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Protocol for Real Time PCR Identification of Anthrax Spores from Nasal Swabs after Broth Enrichment

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Figure

TABLE 1. Strains and plasmids used in the study
TABLE 2. Primers and probes used in real-time PCR
FIG. 2. Qualitative detection of SNPs exploiting changes in Tcirculans(cloned gene; Table 2);containing the target (target ofItalian strains is efficiently detected by theannealed to the SNP containing the target (thems during melting curve analysis
TABLE 3. Autolysis in overnight liquid cultures of B. anthracis

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