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Quantification of karrikins in smoke water using ultra-high performance liquid chromatography–tandem mass spectrometry

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Academic year: 2020

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Figure

Fig. 1 Structures of known karrikin compounds, endogenously occurring in smoke water
Fig. 2 Preparation scheme of karrikins. a TEMPO, NCS, TBAB; b (EtO)2POCH2CO2Et, NaH; c  CF3COOH; d ClCOOEt, pyridine; e  Et3N,  CH2Cl2; f Pd(PPh3)4, THF; g NBS,  CHCl3; h  CH3B(OH)2, Pd(OAc)2, S-Phos, tBuOK, toluene
Fig. 3 Stability of karrikin standards in McIlvaine buffer, pH 5.0. Solutions of  KARwere incubated for 12 days (short-time treatment; d1 (black squares) and  KAR2 (white diamonds) standards  (10−5 M) a, b) and 12 weeks (long-time treatment; c, d), at two
Table 1 Partition coefficients, retention times, diagnostic MRM transitions and  optimised instrument settings for the synthesised and studied karrikins
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