Used together with our Roche Applied Science website, this
Product Index
helps you to
navigate the following product categories:
Ü
Discovery Reagents
Ü
Sample Preparation
Ü
PCR
Ü
Sequencing Solutions
Ü
Cell Biology
Browse this listing of proven instruments and reagent systems for pinpointing when to
perform functional assays that analyze gene and protein expression. Find the
cutting-edge products to identify the cellular and molecular mechanisms used by normal and
diseased cells, with the goal of better understanding how we function. We hope the
solutions provided in this index create breakthroughs and improve laboratory efficiency.
Thank you for using our high quality and outstanding Roche service.
Best wishes,
2. Sample Preparation
3. PCR
Roche Applied Science provides a wide variety
of essentials for research laboratories, that are
complimentary with the analysis technologies referred
in subsequent chapters. Trust the high standard of
quality and reliability. Thoroughly tested reagents with
superb quality are the driving factors for success
deliv-ering performance you essentially need for your tricky
experiments.
Choose from a broad portfolio for hybridization
experiments, cloning, labeling, enzymatic analysis or
simply laboratory buffers and gels. Basic research made
easy.
High quality nucleic acid purification is the gateway to
all genome analysis. Use proven Roche High Pure Spin
Column products and stabilization/extraction reagents for
state-of-the-art nucleic acid purity and yield. To streamline
your laboratory’s automated workflow, use
MagNA Pure
Compact
and
MagNA Pure LC 2.0 Sample Preparation
Systems
.
Proven technology and performance reach the next level in
revolutionary
MagNA Pure 96 Automated Sample
Prepa-ration Systems
. DNA and/or RNA from up to 96 samples
can be isolated in less than one hour from a broad variety
of sample materials using barcoded, prefilled reagents.
Now available for IVD applications.
The new
Roche LightCycler® 96 Real-Time PCR
Instru-ment
combines accuracy and speed with convenient
handling using touchscreens and powerful software for
data analysis. The proven
LightCycler® 480 Real-Time
PCR System
is the preferred end solution for
high-throughput gene expression and genetic variation analysis.
Roche Applied Science has mastered the science of
enzyme blending to bring premium performance to PCR
and RT-PCR products. Choose Roche Applied Science’s
dNTPacks, convenient products that combine PCR-Grade
Nucleotides, thermostable enzymes and enzyme blends,
and all associated products such as buffers and
PCR-enhancing additives in one convenient pack.
5. Cell Biology
Roche’s portfolio of proven DNA sequencing and target
enrichment solutions are advancing research in human
health, agriculture, evolutionary biology and more. The
GS
FLX+ System
and benchtop
GS Junior System
offer the
unique combination of powerful next-generation
sequenc-ing throughput and the familiarity of long Sanger-like read
lengths (up to 1,000 bp).
NimbleGen SeqCap EZ Library products prepare DNA
samples for a variety of next-generation sequencing
platforms allowing researchers to selectively sequence
specific human exome and disease-associated regions.
The broad portfolio of products with complete
customiza-tion enables researchers to minimize sequencing costs in
variant discovery studies.
Improve your cell analysis results. Purify proteins using
cOmplete His-Tag Purification Resin, now applicable
with any buffer with EDTA or DTT for the highest protein
stability without loss of capacity. Proven cOmplete ULTRA
and PhosSTOP Protease Inhibitor Tablets are the easy way
to stabilize the protein you spent days isolating.
Easily transfect a large variety of cell types using the
low cytotoxic X-tremeGENE HP or choose between nine
additional Transfection Reagents. Use Liberase Enzyme
Blends to isolate increased numbers of viable primary cells
with high efficiency.
Ü
Detailed information about products, local contact, terms and conditions:
Online Catalog on
www.roche-applied-science.com
Ü
General Information about Legal Statement and Trademarks:
https://www.roche-applied-science.com/new/legal
Ü
Regulatory and Patent License Disclaimers for individual products:
www.technical-support.roche.com
All 454 Life Sciences’ products mentioned in this catalog are labeled “For life
science research only. Not for use in diagnostic procedures.” For License Disclaimer information of 454 Sequencing
products please refer to the Instructions for Use.
Nucleic Acid Analysis . . . 8
Protein Analysis . . . 23
Antibodies and Detection Substrates . . . 29
Nucleotides and Analogs . . . 33
Catabolic Analysis . . . 37
Metabolite Analysis . . . 41
Laboratory Reagents . . . 41
2
Sample Preparation
. . . .43Automated Sample Preparation of Nucleic Acids . . . 43
Manual Sample Preparation of Nucleic Acids . . . 50
Isolation of Cells - Tissue Dissociation . . . 53
Degradation of Cellular Components . . . 54
Isolation of Proteins . . . 55
3
PCR
. . . .60 Real-Time PCR Systems . . . 60 Real-Time PCR Reagents . . . 75 PCR/RT-PCR Enzymes . . . 76 GS Junior System . . . 82 GS FLX+ System . . . 83 GS FLX System . . . 84 Assays . . . 85 Sequence Capture . . . 865
Cell Biology
. . . .88Cell Counter and Analyzer . . . 88
Fluorescence based Cellular Assays . . . 91
Transfection . . . 91
Cell Culture Reagents . . . 92
Mycoplasma Detection and Elimination . . . 94
Growth Factors and Cytokines . . . 94
Reporter Gene Detection . . . 95
Apoptosis . . . 96
Cytotoxicity . . . 98
Cell Proliferation . . . 99
Oncology Research . . . 100
Virus Research . . . 100
6
Alphabetical Product Index
. . . .101Nu
Lab
Kits
Prod High Conv solut tripho kit fo Nick Labe of the the n DNA Rand Unifo modi many South SP6/ For in SP6 o nonra hybriEnz
Prod DNA This conta trans Klen Use t techn in rec oligo Klen This s Sang be us the e Polyn Use t DNA phos witho SP6 Trans prom Use l prote T3 R Trans prom Use l synth T4 D Labe hybri must T4 Geucleic Acid
beling of Nu
s for Labeling
duct Nameh Prime DNA Lab
venient, rapid rand ions offer a choice osphates (32P, 35S or many different h
k Translation Kit
l DNA with radioa e helix, incorporati nicked site. Use lab /RNA transfer hyb
dom Primed DNA
ormly label plasmid fied dNTP. High sp y hybridization tech hern and northern
/T7 Transcription
n vitro transcriptio
or T7 promoter. Sy adioactively labele dizations, genomic
ymes for Lab
duct Name
A Polymerase I
recombinant DNA ain DNase I). It can slation (with added
ow Enzyme
this labeling-grade niques, such as 3’-cessed DNA ends nucleotides during
ow Enzyme
sequencing grade ger DNA sequencin sed in 3’-end label elongation of oligon
nucleotide Kinas
to phosphorylate 5 or RNA using [γ
phate exchange, a out altering their 5
RNA Polymerase
scribe RNA from c moter. Perform synt abeled RNA in DN ection, and synthes
RNA Polymerase
scribe RNA from c moter. Perform synt abeled RNA in RN hesis of capped RN DNA Polymerase l 3'-termini of DNA dization probe. DN t have 5´-protrudin ene 32 Protein in s
d Analysis
cleic Acids
g of Nucleic A
eling Kit om-primed DNA l e in the selection o S, 3H, digoxigenin, ybridization techn ctive or modified d ing labeled nucleo beled probes in co bridization, and ISHA Labeling Kit
d or phage DNA w pecific activity labe hniques, including blots, and in situ
n Kit on of DNA sequenc ynthesize labeled R ed ribonucleotides. c sequencing, and
beling of Nucl
A Polymerase I is en n therefore be use d DNase I) and sece enzyme from E. c
-end labeling, rand to create blunt-en g site-directed mut enzyme is specific ng (dideoxy-chain
ing, random DNA nucleotides.
se
5' ends of DNA or -32 P]-ATP either b
and to remove 3'-p ' ends (at low pH o
e
cloned DNA templa thesis with labeled NA/RNA blots, in s sis of capped RNA cloned DNA templa thesis with labeled NA/DNA blots, ISH NA in vitro with m7 A, producing highl NA labeled with re ng ends. Blunt-end site-directed mutag
s
Acids
abeling kit. Suppli of modified deoxyr fluorescein, biotin iques. dNTPs. Enzymes n otides as polymeras lony/plaque hybrid H. with any [α-32 P]-d eled DNA probes a g gene library scree
hybridizations. ces cloned downst RNA using radioact . Use in DNA/RNA S1 nuclease stud
leic Acids
ndonuclease-free d for labeling of D cond-strand synthecoli in many molec dom primed DNA l nded DNA, and elo
tagenesis. cally prepared and
termination metho labeling, fill-in rea
RNA, label 5'-hydr by direct phosphor phosphates from R only). ates downstream f d NTPs to generate situ hybridization, R A in vitro. ate downstream fr d NTPs, generating , RNase protection 7GpppG or m7Gpp ly labeled DNA for ecombinant T4 DNA d DNA will not wor
genesis.
ed nucleotide ibonucleoside n, etc). Use this
ick one strand se proofreads dization, dNTP or are used in ening, tream of the tive or A ies. (does not DNA by nick esis of cDNA. cular biology labeling, filling ongation of d tested for od). It can also actions, and roxyl ends of rylation or by RNA or DNA from an SP6 e labeled RNA. RNase rom a T3 g labeled RNA. n, and ppA. r use as A Polymerase rk. Use with Cat. No. 11 585 584 001 10 976 776 001 11 004 760 001 10 999 644 001 Cat. No. 10 642 711 001 10 642 720 001 11 008 404 001 11 008 412 001 10 104 531 001 10 174 645 001 10 633 542 001 10 810 274 001 11 487 671 001 11 031 163 001 11 031 171 001 11 004 786 001 11 004 794 001 1 kit for up labeled nucleo 1 kit for up to 1 kit for 1 kit for up t 2 Pack Size to 50 labeling reac otide of choice, 0.0 per assay o 50 standard labe up to 50 labeling r to 2 x 20 transcript Pack Size 250 U 1,000 U 100 U 500 U 250 U (5 x 103 U/m 200 U 1,000 U 1,000 U 5,000 U 1,000 U 5,000 U 100 U 500 U ctions with a 01 to 2 µg DNA eling reactions reactions tion reactions ml) Price in € 481,00 389,00 431,10 559,00 Price in € 139,20 462,90 104,00 417,80 268,30 206,90 823,00 106,60 412,80 106,60 412,80 194,40 735,10
T4 P Phos RNA 3'-CM label T7 R Trans prom Use R micro Term Add fragm and 3 ddNT
PCR
Prod PCR Gene low-c Probe remo PCR For d PCR. in PC produ PCR Direc PCR. diges Urac PCR Add hybri when ELISA PCR Conv produ conve Micro PCR Conv 100 t ELISA sequ PCR Conv produ (see contr Hybr Use i ELISA olynucleotide Kin phorylate 5'-hydro and DNA, 3' ends MP, forming 5'[32P] ing of RNA with T4
RNA Polymerase
scribe RNA from c moter. Perform synt RNA in RNA/DNA oarray target synth
minal Transferase
nucleotides to the ments, such as for
3´-end labeling wit TPs (e.g ., DIG-ddU
R Product L
duct Name
DIG Probe Synth
erate highly sensiti copy gene detectio es labeled with alk oval of digoxigenin
DIG Labeling Mi
direct labeling of am The PCR DIG labe CR. The nucleotide uct combined with
DIG Labeling Mi ct labeling of ampl Prevent PCR carry st contaminating P il-DNA Glycosylas Fluorescein Lab mix directly to PCR dization probes. P n limited amounts A, membrane hybr ELISA, DIG-Labe venient nonradioac ucts for detection entional ethidium oplate format is ide
ELISA, DIG-Dete
venient, nonradioac times more sensitiv A format makes de ence in large sam
ELISA, DIG-Dete
venient, nonradioac ucts; 5-pack is the Cat. No. 11636111 rols. For parallel te
ridization Buffer
n combination wit As for application
nase, 3'-phospha
oxyl ends of oligon s of RNA, and add ]pCp. This substra 4 RNA ligase. cloned DNA templa
thesis with labeled blots, ISH, RNase hesis, and synthesi
e 3´-OH ends of do homopolymeric ta th either radioactiv UTP).
Labeling and
hesis Kit ve hybridization pr on of rare mRNA in kaline-labile digoxilabel after detecti
ix
mplification produ eling mix can repla concentration ens h DIG detection. ixPLUS ification products yover by incorpora PCR products from e. beling Mix Rs to produce fluo articularly useful f of target DNA is a ridization, or ISH. eling
ctive method adds using PCR ELISA; bromide staining o eal for automation
ection
ctive method for la ve than ethidium b etermining presenc ple numbers easy.
ection, 5-pack
ctive method for la e high capacity ver 910), providing mo sting of a large nu th the PCR ELISA a in nucleic-acid hyb
atase free
ucleotides and link a 5'[32 P]-terminal ate is commonly us ate downstream fr d NTPs, generating protection, s of capped RNA uble- or single-str ailing or end-labeli ve or chemically m
d Detection
robes using PCR. S n Southern and no igenin (DIG)-dUTP ion are stable for o cts using digoxige ace the unlabeled sures maximum yieusing digoxigenin ating dUTP instead
previous amplifica
rescein-labeled DN for synthesizing DN available. Use label
a DIG label to you 100 times more se of products using n.
abeling and detect bromide staining o ce or absence of s
abeling and detect rsion of the standa ore reagents, but l umber of samples. and the series of D bridization reactio
kers, 5' ends of label to sed for 3'-end
rom a T7 g labeled RNA. in vitro . randed DNA ng with dNTPs, modified Suitable for orthern blots. P for easy over one year. enin-dUTP in nucleotide mix eld of PCR (DIG)-dUTP in of dTTP and ations using NA NA probes led probes in ur PCR ensitive than agarose gels. t PCR products; n agarose gels. specific target ting PCR ard kit acking DIG-detection ns. 10 709 557 001 10 838 292 001 10 881 767 001 10 881 775 001 03 333 566 001 03 333 574 001 Cat. No. 11 636 090 910 11 585 550 910 11 835 289 910 11 636 154 910 11 636 120 910 11 636 111 910 11 965 409 910 11 717 472 001 8,000 U (fo reacti 24,000 U (fo reacti 1 kit for up to 2 volume (one labeled pro 500 µl (2 x 250 reactions of 1 co 2 x 250 µl for u 50 µ 100 µl for up t 1 kit 1 kit for 1 microplates detec 1 kit fo (five microplat on the number standar 200 U 1,000 U 1,000 U 5,000 U or 20 tailing or 3'-e ons, 400 U per rea or 60 tailing or 3'-e ons, 400 U per rea
Pack Size
25 reactions of 50 e reaction can prod obe to analyze 650 membrane) 0 µl) for up to 2 x 2 00 µl final reaction oncentration 200 µ up to 2 x 50 reactio µl final reaction vol
to 10 PCR reaction 100 µl
t for up to 50 react
192 detection react s), allows the semi-ction of 50 PCR pro
or 480 detection re tes), the number of r of required samp rd and/or control r 100 ml end labeling action) end labeling action) µl final reaction duce enough cm2 of blot 25 PCR assays, n volume, final µM ons, reactions of lume ns, reactions of tions tions (two -quantitative oducts eactions f tests depends le dilutions and eactions 292,20 902,00 106,60 412,80 95,30 214,50 Price in € 435,70 394,70 394,70 322,70 548,60 502,10 867,90 76,90
Pro
Kits
Prod DIG Nonr DIG-and t north DIG-Comp DIG-of lab South DIG-Comp chem labele north DIG Produ chem Desig reageKits
Prod DIG Nonr DIG-gene chrom DIG Comp (SP6/ effici and R DIG 3'-en using oligo mem DIG For 5 oligo slot b DIG For fa DIG-tailed memMix
Prod High Labe prime librar G-50 Bioti Labe polym north and ioducts for M
s for Labeling
duct Name DNA Labeling an radioactive random 11-dUTP, followed the substrates NBT hern, and dot blots-High Prime DNA
plete, convenient k 11-dUTP and colo beled probes in 1 h hern/northern blot
-High Prime DNA
plete, convenient k milumiluminescent ed probe in 1 hour hern blots, colony/p
Northern Starter
uce DIG-labeled R miluminescent dete gned for the novice ents proven to pro
s for Labeling
duct Name
DNA Labeling Ki
radioactive random 11-dUTP. Used for detection. Detect mogenic or chemil
RNA Labeling Ki
plete kit for RNA la /T7). DIG-labeled r ency. Used in nort RNase protection e Oligonucleotide nd labeling of oligo g DIG-11-ddUTP a nucleotides are us brane-blot applica Oligonucleotide 5'-end labeling of o nucleotides are us blots, colony/plaqu Oligonucleotide
ast and sensitive ta 11-dUTP and reco d with DIG-dUTP a brane blots or in s
xes and Reag
duct Name
h Prime
l DNA with radioa ers. Labeled probe ry screening, and i
to remove uninco
in RNA Labeling
l RNA with Biotin-merases (SP6, T3, a hern, and dot blots
in situ and microa
Membrane H
g and Detectio
nd Detection Kit
m primed labeling o d by color detection T and BCIP. Use la s, and colony and p
A Labeling and De
kit for random prim r detection of labe hour to overnight i ts, and colony/plaq
A Labeling and De
kit for random-prim detection of labele r to overnight incu plaque hybridizatio
r Kit
RNA probes to use ection reagents for e DIG system user vide successful, re
g
it
m primed labeling o r in situ and filter h
labeled hybrids us luminescence AP s
t (SP6/T7)
abeling using DIG-run-off transcripts thern/Southern blo experiments. 3'-End Labeling onucleotides from nd recombinant Te sed as hybridizatio ations and in situ h
5'-End Labeling
oligonucleotides w sed in hybridization ue, and in situ .
Tailing Kit, 2nd g
ailing of oligonucle ombinant Terminal are used for DNA o
situ hybridization.
ents for Labe
ctive dCTP using r es are used in Sout
in situ hybridizatio
orporated nucleotid
Mix
-16-UTP by in vitro and T7). Labeled R s, plaque and colon rray hybridizations
Hybridization
on
of DNA probes usi n using anti-DIG-A abeled probes in So plaque screening.
etection Starter
med DNA labeling eled hybrids. Obtai incubation. Use in que hybridizations.
etection Starter
med DNA labeling ed hybrids. Obtain ubation. Use in Sou
on.
with the supplied r northern blotting r. This Starter Kit c eproducible results
of DNA probes wit hybridizations and sing anti-DIG-AP c substrates. -11-UTP by in vitro
are synthesized w ots, ISH, plaque or
Kit, 2nd generati
14 to 100 nucleotid erminal Transferas
n probes in all typ hybridization.
Set
with digoxigenin. DI ns, such as Southe
generation
eotides at the 3'-e Transferase. Oligo or RNA hybridizatio
eling
random oligonucle thern northern, an ons. Use Quick Spi
des.
o transcription usin RNA can be used i ny lifts, RNase prot s.
n
ing AP conjugate outhern, Kit I using n high yields . Kit IIwith DIG and n high yield of uthern/ techniques. ontains the s. th d single-copy conjugate and o transcription with high colony lifts, ion des in length se. DIG-labeled pes of IG-labeled ern, dot and
nd using onucleotides on in eotides as d dot blots, n Columns, ng RNA n Southern, tection assays, Cat. No. 11 093 657 910 11 745 832 910 11 585 614 910 12 039 672 910 Cat. No. 11 175 033 910 11 175 025 910 03 353 575 910 11 480 863 001 03 353 583 910 Cat. No. 11 585 592 001 11 685 597 910 1 kit for up detection of 5 1 kit for up detection of 2 as 1 kit for up detection of 2 1 kit for up detection o reactions of 1 1 kit for up to 4 1 kit for up 1 kit for up to 2 oligonucleotide of a 1 set for a 1 kit for up to oligonucleotide of a 200 µl fo 40 µl for up Pack Size p to 25 labeling rea 50 blots, 10 ng to 3 blots of 100 cm2 p to 12 labeling rea 24 blots, 10 ng to 3 ssay, blots of 100 c p to 12 labeling rea 24 blots, 10 ng to 3 blots of 100 cm2 p to 10 labeling rea f 10 blots, blots of 1µg DNA, yielding labeled RNA, each
Pack Size
0 labeling reaction DNA per assay
p to 2 x 10 labeling
25 labeling reactio s per assay corres 30-mer oligonucle
at least 10 labeling
o 25 tailing reactio e per assay corresp 30-mer oligonucle Pack Size or up to 50 labelin p to 20 transcriptio actions and 3 µg per assay, actions and 3 µg DNA per cm2 actions and 3 µg per assay, actions and f 10 x 10 cm2 approx. 20 µg h ns, 10 ng to 3 µg g reactions ons, 100 pmol of sponding to 1 µg eotide g reactions ons, 100 pmol ponding to 1 µg eotide g assays on reactions Price in € 763,20 461,70 469,70 484,00 Price in € 596,80 562,80 544,40 414,30 506,10 Price in € 364,60 176,00
Bioti Comp probe after in situ DIG Easy-Dig-1 lifts, o dGTP DIG Mixtu (SP6/ and R GTP DIG-Prem Obta incub dot b Digo hydr For D via am with group Fluor RNA T7, o enzym (Sout Fluor Conv prime at hig lifts, Hexa This prime in ma and n
Kits
Prod DIG Conta acid DIG-conju DIG High South chem methHyb
Prod DIG Read using Hyb b nonto DIG Block DIG-for 10 made in-High Primeplete reaction mix es. Biotin-labeled overnight incubat
u, and colony and
DNA Labeling M
-to-use nonradioac 11-dUTP. For Sout or in situ hybridiza P (each), 0.65 mM
RNA Labeling M
ure for RNA labelin /T7/T3). Used in no RNase protection e (each), 6.5 mM UT
-High Prime
mixed solution for ra in high yields of la bation. Use labeled blots, and colony/p
oxigenin-3-O-me oxysuccinimide e
DIG labeling of pro mino groups (unde NH2 -groups of pro
p, digoxigenin, is b
rescein RNA Lab
labeling with Fluo r T3 RNA polymera me-conjugated an therns, northerns, rescein-High Pri venient enzyme an ed labeling of DNA gh yield for use in ISH, and library sc
anucleotide Mix
10x Hexanucleotid ed DNA labeling. H any hybridization t northern blots, and
s for Detectio
duct Name
Nucleic Acid Det
ains all reagents to hybrids. Used in S labeled nucleic ac ugate and the colo
Luminescent Det
ly sensitive, specif hern/northern blot miluminescent subs hods, with shorter e
bridization Re
duct Name Easy Hyb dy-to-use blocking g nonradioactive, d buffer cannot be u oxic solution and dEasy Hyb Granul
king buffer for mem labeled nucleic ac 00 ml of a 1x work e solutions and lon
for efficient rando probes are genera ion. Use in Southe plaque lifts.
Mix
ctive rapid random hern, northern, an ation; 10x solution dTTP, 0.35 mM DI ix ng using DIG-11-U orthern/Southern b experiments; 10x s TP, 3.5 mM DIG-11 andom primed DN abeled probes with d probes in library plaque hybridizatio
ethylcarbonyl-ε -ester
teins and 5'-amino er gentle condition oteins or oligonuc bound by Anti-Dig
beling Mix
orescein-12-UTP by ases. Detect labele ti-fluorescein, and plaque lifts, ISH).
me
d nucleotide mix f A with
fluorescein-Southern, norther reening. de Mix has all poss
High specific activ echniques for scre d in situ hybridizat
on
tection Kit
o detect membran Southern, northern, cids are detected u or substrates NBT a tection Kit ic detection of DIG ts and plaque/colo strate CSPD. Signa exposure times.
eagents
buffer for all mem digoxigenin-labeled used for in situ hyb
does not contain fo
es
mbrane hybridizat cid probes (not for king solution; 6 x 1 ng-term storage.
om primed labeling ated at high yield w ern, dot, and northe
m primed labeling m d dot blots, colony
with 1 mM dATP, G-11-dUTP. UTP by in vitro tran
blots, ISH, plaque solution of 10 mM
-UTP.
NA labeling using D hin one hour or ove
screening, Southe ns.
-aminocaproic ac
o-substituted oligo ns). One reactive g leotides. The other oxigenin and dete y in vitro transcrip
ed RNA directly or d use in hybridizati
or highly efficient -dUTP. Generate la n, and dot blots, co
sible sequences fo ity labeled DNA pr eening gene librari tion.
e-blotted, DIG-lab , and dot blots, and using anti-DIG-AP
and BCIP. G-labeled nucleic a ony lifts with anti-D al is as sensitive as
mbrane hybridizatio d nucleic acid pro bridization. The bu ormamide.
ion using nonradio ISH). Dissolve gra 00 ml portions allo g of DNA within 1 hour or ern blotting, mix with y and plaque dCTP, nscription or colony lifts, ATP, CTP, DIG-11-dUTP. ernight ern, northern, cid-N-onucleotides group interacts r functional cted. ption using SP6, r with an on techniques random abeled probes olony/plaque r random robes are used ies, Southern
beled nucleic d ISH. antibody
acids in DIG-AP and the
s radioactive
on applications bes. DIG Easy ffer is a oactive nules in water ow for freshly 11 585 649 910 11 277 065 910 11 277 073 910 11 585 606 910 11 333 054 001 11 685 619 910 11 585 622 910 11 277 081 001 Cat. No. 11 175 041 910 11 363 514 910 Cat. No. 11 603 558 001 11 796 895 001 100 µl for up t tem 50 µl for 40 µ 160 µl for up t tem 40 µ 100 µl for up t tem 100 µ 1 kit for up 1 kit for u gr o 25 labeling assay mplate DNA per as
up to 25 standard
µl for up to 20 reac
o 40 labeling assay mplate DNA per as
5 mg
µl for up to 20 reac
o 25 labeling assay mplate DNA per as
µl for up to 50 reac Pack Size p to 40 blots of 10 up to 50 blots of 1 Pack Size 500 ml ranules for 6 x 100 ys, 0.01 to 3 µg ssay reactions ctions ys, 0.01 to 3 µg ssay ctions ys, 0.01 to 3 µg ssay ctions cm x 10 cm 0 x 10 cm ml 334,90 156,70 160,70 533,50 186,20 160,70 410,20 57,50 Price in € 393,50 515,60 Price in € 266,00 305,80
DIG W The D hybri block Bloc Block filter Block
Mem
Prod Nylo Matr (DIG) RNA, Stron hybri Nylo Unch phag chem biotin Hybr Use t stand proce sequPro
Mix
Prod DIG Mixtu (SP6/ and R GTP Fluor RNA T7, o enzym (Sout Nick Optim hybri to de chrom DIG-Conv labele for fil the D Bioti Enzym Biotin for fil HighWash and Block
DIG Wash and Blo dization and DIG d king solution and m
cking Reagent
king Reagent is us hybridization and king reagent does
mbranes
duct Name
on Membranes, p
ix for hybridization ) and biotin, or rad , or oligonucleotide ngest signals and l
ds. on Membranes fo harged microporou ge/cosmid libraries miluminescent/chro nylated or radioact ridization Bags
these bags in nonr dard radioactive pr edures. All hybridiz entially to the sam
oducts for
in
xes for Label
duct Name
RNA Labeling M
ure for RNA labelin /T7/T3). Used in no RNase protection e (each), 6.5 mM UT
rescein RNA Lab
labeling with Fluo r T3 RNA polymera me-conjugated an therns, northerns,
k Translation Mix
mized enzyme mix dization. Designed etect multicopy or v msomes or interph -Nick Translation venient enzyme nu ed using DIG-11-d lter hybridization. F DIG-High Prime kit
in-Nick Translati
me nucleotide mix n-16-dUTP for use lter hybridization. F
Prime.
Buffer Set
ck Buffer Set is us detection. The set maleic acid, washin sed to decrease the the detection of n not contain BSA.
positively charged
n with nonradioact dioactive labeling u e probes, for Sout owest background
or Colony and Pla
us nylon membran s with DIG probes a
omogenic substrat tive probes. Pore s radioactive hybridi robe hybridizations zation solutions an me bag.
n situ
Hybrid
ing
ix ng using DIG-11-U orthern/Southern b experiments; 10x s TP, 3.5 mM DIG-11 beling Mix orescein-12-UTP by ases. Detect labele ti-fluorescein, and plaque lifts, ISH).x
generates sensitiv d for direct fluorop very large hybridiz hase nuclei.
n Mix
cleotide mix to ge dUTP for in situ hy For highly sensitive ts.
on Mix
xture for generating e in in situ hybridiz
For highly sensitive
sed at various step contains 10x prep ng, and detection e background in n ucleic acid hybrids
d
tive labeling using using 32 P, 35 S, 3 H
thern, northern an d obtained with DI
aque Hybridizatio
e for nonradioactiv and detection with tes. Use in hybridiz size: 1.2 µm.
zation and detecti s, and western blo nd blocking buffer
dization
UTP by in vitro tran blots, ISH, plaque solution of 10 mM
-UTP.
y in vitro transcrip ed RNA directly or d use in hybridizati
ve probes for fluor phore-labeling of in
zation targets on m
nerate highly sens ybridization. The m e filter hybridizatio
g sensitive probes zation. The mix can e filter hybridizatio s of DIG arations of buffers. onradioactive s. This Roche digoxigenin with DNA, d dot blotting. G-labeled on ve screening of h zations with on procedures, tting can be added nscription or colony lifts, ATP, CTP, ption using SP6, r with an on techniques escence in situ n situ probes, metaphase sitive probes mix can be used
on probes, use
labeled with n also be used on, use
Biotin-11 585 762 001 11 096 176 001 Cat. No. 11 209 272 001 11 209 299 001 11 417 240 001 11 699 075 001 11 699 083 001 11 666 649 001 Cat. No. 11 277 073 910 11 685 619 910 11 745 808 910 11 745 816 910 11 745 824 910 1 10 20 50 disc 50 discs 40 µ 40 µ 200 µl for 160 µl for 160 µl for
set for up to 30 blo
50 g Pack Size 0 sheets (20 x 30 c 0 sheets (10 x 15 c 1 roll (0.3 x 3 m) cs (each 82 mm dia s (each 132 mm d 50 bags Pack Size µl for up to 20 reac µl for up to 20 reac r up to 50 labeling r up to 40 labeling r up to 40 labeling ots cm) cm) ameter) iameter) ctions ctions reactions reactions reactions 251,80 84,40 Price in € 403,00 224,00 525,80 268,80 500,30 102,40 Price in € 160,70 160,70 333,90 484,60 484,60
Ma
Res
Prod Aat I Aat I with recog Ssp 5 Acc Acc I fragm N6-m sequ Afl II Afl III fragm 5-me Afl III Alu I Alu I blunt 5-hyd Isosc Apa Apa with indic Bsp 1 Asp7 Reco with meth inhib Asp7 Reco fragm 5-me not in Bam Reco 5'-co meth resid BbrP Reco blunt recog Eco7 Bcl I Reco 5´-co 6N-p 5-me Bfr I Reco 5´-co 5-me Bst98apping and C
striction Enzy
duct Name II I recognizes the se 3´-cohesive termin gnition sequence a 5230 I and Zra I. I I recognizes the se ments with 5´-cohe methyladenine and ence. Acc I is an isII
I recognizes the se ments with 5’-cohe ethylcytosine at the I has no known iso
I recognizes the se t termini. It is inhib droxymethylcytosin chizomer: AluB I. I I recognizes the se 3´-cohesive termin ated (*) in the reco 120 I and Psp OMI
700 I (Xmn I)
ognizes the sequen blunt termini. Inhib hyladenine at the o
it. Isoschizomers:
718 I
ognizes G↓GT°AC*C
ments with 5’ cohe ethylcytosine as ind
nfluence activity(°)
mH I
ognizes the sequen ohesive termini. Ba hylation, but is inhi ue as indicated (*)
P I (PmaC I)
ognizes the sequen t ends. The enzyme gnition site as indic 2 I, PmaC I, Pml I, ognizes the sequen ohesive termini. Inh position of adenine ethylcytosine. Isosc
(Afl II)
ognizes the sequen ohesive ends. The e ethylcytosine at the 8 I, MspC I, and Vh
Cloning of N
mes
equence GA*CGT↓ ni. It is inhibited by as indicated (*). Aa equence GT↓(A,C) esive termini. It is i 5-methylcytosine soschizomer to Fb equence A↓*C(A,Gesive ends. Afl III is e site indicated (*) oschizomers. quence *AG↓*CT a bited by 6-methylad ne,or 4-methylcyto equence GGG*CC↓ ni. It is inhibited by ognition sequence . nce G*A°ANN↓NN bited by 6-methyla other A(°) or presen MroX I, Pdm I, Xm C; same sequence sive ends, not 3’ e dicated (*). Methyl ). Neoschizomer: K nce G↓G°AT*C°C a m H I is not inhibit bited by 5- or 4-m ). Isoschizomer: Bs
nce CA*C↓GTG and
e is inhibited by 5-cated (*). BbrP I is and PspC I. nce T↓G*AT*CA, ge hibited by the dam e. Inhibited by 5-hy chizomers: Fba I, K
nce *C↓TTAAG and
enzyme is inhibited e site indicated (*). ha464 I.
NA
↓*C and generates y 5-methylcytosine at II is a neoschizo(T,G)*A*C and gen nhibited by the pre as indicated (*) on l I and Xmi I. G)(T,C)GT and gene
s inhibited by the p on the recognition
and generates frag denine, 5-methylcy osine at the (*) in th ↓*C and generates y 5'-methylcytosine e. Apa I is a neosch TT°C and generate adenine at the (*). nce of 5-methylcyt mn I. e as Kpn I but gene nds as from Kpn I lation of A and cen Kpn I.
and generates frag ted by overlapping methylcytosine at th stI. d generates fragm -methylcytosine wi s an isoschizomer o enerating fragmen m gene and methyla
ydroxymethyl-cytos Ksp22 I. d generates fragm d by the presence . Isoschizomers: Af fragments e in the omer of nerates esence of n the erates presence of n sequence. gments with ytosine, he sequence. s fragments e at the sites hizomer to es fragments
6-tosine does not
erates . Inhibited by ntral C does ments with g dam he internal C ments with ithin the of Acv I, nts with ates the sine, but not by
ents with of fl II, BspT I, Cat. No. 10 775 207 001 10 728 438 001 11 209 183 001 10 239 275 001 10 656 267 001 10 703 753 001 10 899 208 001 10 835 277 001 10 814 245 001 10 814 253 001 11 175 050 001 10 220 612 001 10 567 604 001 10 656 275 001 10 798 975 001 11 274 031 001 11 168 860 001 10 693 952 001 11 097 059 001 11 198 939 001 Pack Size 250 U (1 - 5 U/µl) 500 U (5 U/µl) 100 U (5 U/µl) 500 U (10 U/µl) 2,000 U (10 U/µl) 20,000 U (40 U/µl) 5,000 U (10 U/µl) 500 U (10 U/µl) 1,000 U (10 U/µl) 5,000 U (10 U/µl) 5,000 U (40 U/µl) 1,000 U (10 U/µl) 2,500 U (10 U/µl) 10,000 U (10 U/µl) 10,000 U (40 U/µl) 50,000 U (40 U/µl) 500 U (10 U/µl) 500 U (10 U/µl) 2,500 U (40 U/µl) 500 U (10 U/µl) ) ) ) ) ) Price in € 52,00 411,30 255,10 76,90 222,90 310,10 95,50 72,10 66,50 277,30 277,30 28,80 56,50 194,20 194,20 788,00 89,20 66,50 170,80 63,20
Bgl I Reco 5´-co but is Bgl II Bln I Bln I 5´-co meth BseA Reco 5'-co indic Acc I Bsm Reco 3'-co sequ Isosc BssH Reco 5´-ov gene indic BstX Reco with 5-me enzym Cfo I Reco 3´-co by sim Isosc Cla I Reco 5'-co 5-me BshV Dde Dde 5´-co 5´-hy HpyF Dpn Reco ends at the Mal I Dra I Dra I blunt the s isosc Dra I Dra I fragm (*). Is EclX Reco 5´-co show BseX II
ognizes the sequen ohesive termini. It i s sensitive to 5-me I has no known iso
I (Avr II)
recognizes the seq ohesive termini. Th hylation. Bln I is an
A I
ognizes the sequen ohesive termini. Sen
ated (*), whereas t III, Aor13H I, Bsp13
I
ognizes the sequen ohesive termini.Inh ence as indicated chizomers: BsaM I,
H II
ognizes the sequen verhanging ends. B rate large fragmen ated (*). Isoschizo
X I
ognizes the sequen 3´-overhanging en ethylcytosine within me has no known
I (Hha I)
ognizes the sequen ohesive termini. Inh multaneous presen chizomers: AspLE I
I
ognizes the sequen ohesive termini. It is ethylcytosine as sh V I, BspD I, BspX I, I I recognizes the se ohesive termini. It i ydroxymethylcytosi F3 I. I
ognizes the sequen . The enzyme is on e indicated site (*) I.
I
recognizes the se t ends. Dra I is not ite indicated (°) on chizomer of Aha III
III
II recognizes the s ments with 3´-cohe soschizomer: Ade I
I (Xma III)
ognizes the sequen ohesive termini. Inh wn (*). 5-methylcyto X3 I, BstZ I, Eag I, E
nce A↓G°AT*CT an
s not inhibited by ethyl and 5-hydrox oschizomers.
quence C↓CTAGG
e enzyme is not kn isoschizomer of A
nce T↓CCGG*A and
nsitive to overlapp the isoschizomer M 3 I, BspE I, Kpn2 I, nce G*AATG°CN↓N ibited by 6-methyl (*). 5-methylcytos Mva1269 I, Pct I. nce G↓*CG*CGC an Belongs to a class nts of DNA. Inhibit mers: BseP I, Pau
nce *CC*A(N)5↓NT nds. BstX I is inhib n the recognition s isoschizomers. nce G*CG↓*C and g hibited by 5-methy nce of 5-hydroxym , BstHH I, Hha I. nce *AT↓*CG*AT a
s inhibited by over own (*). Isoschizo Bsu15 I, BsuTU I.
equence *C↓TNAG
s inhibited by the ine at the site indic
nce GmA↓T*C and
nly inhibited by the if no 6-methylade equence TTT↓A°AA inhibited by the p n the recognition s . sequence C*A*CNN esive termini. It is m I. nce *C↓GG*C*CG a hibited by 5-methy osine in position 4 Eco52 I. nd generates fragm overlapping dam m xy-methylcytosine,
G and generates fra nown to be affecte Avr II, AspA2 I, and
d generates fragm ping dam methylati Mro I is not. Isosch
Mro I. N and generates fr
adenine within the ine does not inhib
nd generates fragm of rare-cutter enzy ted by 5'-methylcyt I. TGG and generates ited by 6-methylad sequence as indica generates fragmen ylcytosine, as show methylcytosine at b nd generates fragm rlapping dam-meth
mers: Ban III, Bsa2
G and generates fr presence of 5´-me cated (*). Isoschizo generates fragme e occurrence of 5-enine is present. Is A and generates fr presence of 6-meth sequence. Dra I is NN↓GTG and gene methlation sensitiv
and generates frag ylcytosine in positio 4 also inhibits. Isosc
ments with methylation (°), as shown (*). agments with ed by d Xma I. ments with ion as hizomers: ragments with e recognition it (°). ments with ymes, which tosine as s fragments denine and ated (*). The nts with wn (*). Inhibited oth C residues. ments with hylation and 29 I, BseC I, agments with ethyl- or omers: BstDE I,
ents with blunt methylcytosine oschizomer: ragments with hyladenine at an erates ve as indicated gments with on 1 and 5 as chizomers: 10 348 767 001 10 567 639 001 10 899 224 001 11 175 068 001 11 558 161 001 11 558 170 001 11 417 169 001 11 292 307 001 11 168 851 001 11 117 777 001 11 117 785 001 10 688 541 001 10 404 217 001 10 656 291 001 11 092 758 001 10 835 307 001 10 742 970 001 10 742 988 001 10 827 754 001 10 843 547 001 11 131 397 001 500 U (10 U/µl) 2,000 U (10 U/µl) 2,000 U (40 U/µl) 10,000 U (40 U/µl) 200 U (10 U/µl) 1,000 U (10 U/µl) 200 U (10 U/µl) 200 U (10 U/µl) 200 U (10 U/µl) 250 U (10 U/µl) 1,250 U (10 U/µl) 1,000 U (10 U/µl) 500 U (10 U/µl) 2,500 U (10 U/µl) 2,500 U (40 U/µl) 1,000 U (10 U/µl) 200 U (10 U/µl) 1,000 U (10 U/µl) 5,000 U (10 U/µl) 500 U (1 - 5 U/µl) 1,000 U (10 U/µl) ) ) 66,50 177,50 177,50 521,30 128,60 515,80 102,00 112,10 112,10 63,20 255,10 50,20 73,20 291,80 291,80 167,50 83,20 330,50 139,80 267,30 478,00
Eco4 Eco 4 with as ind Aor5 EcoR Eco R with or bo isosc EcoR Reco blunt (*). It 5-hyd Fok I Reco 5'-co dista Neos Hae Reco ends C*. In Isosc Hind Reco blunt inhib recog Hind Reco 5´-co 5-hyd inhib Hinf Reco 5´-co 5-me 5-hyd Hpa Reco blunt as ind Isosc Kpn Reco 3´-co both Ksp Belon fragm gene of 5'-Mae Reco 5’-co inform FspB Mae Reco 5´-co Inhib Isosc 47 III 47 III recognizes th blunt ends. Eco47 dicated (*). 6-mety 1H I.
R I
RI recognizes the s 5´-cohesive termin oth A residues, and chizomer to Rsr I.
R V
ognizes the sequen t ends. Inhibited by is not inhibited by droxymethylcytosin
I
ognizes the sequen ohesive termini. A C
nces from the seq schizomers: BseG I
III
ognizes the sequen . Inhibited by 5-me nhibited by 5-hydro chizomers: BshF I,
d II
ognizes the sequen t ends. Inhibited by ited by 5-hydroxym gnition sequence.
d III
ognizes the sequen ohesive termini. Inh droxymethylcytosin it (°). Isoschizome
I
ognizes the sequen ohesive termini. Se ethylcytosine at 3’p droxymethylcytosin
I
ognizes the sequen t ends. Inhibited by dicated (*). 5-meth chizomers: KspA I.
I
ognizes the sequen ohesive termini. Kp C-residues or by 6 I (Sac II) ngs to a class of ra ments of genomic rates fragments w -methylcytosine. Is e I
ognizes the sequen ohesive termini. Su mation is available I, Bfa I, Xsp I.
e II
ognizes the sequen ohesive termini. Su bited by the presen chizomers: HpyCH4
he sequence °AG*C III is inhibited by t yladenine is not inh
sequence G↓*A*AT
ni. EcoR I is inhibite d by 5-methylcytos
nce G*AT↓AT°C an
y the presence of 6 y the presence of 5 ne. Isoschizomer: E nce GG*ATG(N)9/1 Class II S enzyme w uence. Inhibited b I, BstF5 I, BtsC I. nce GG↓*C°C, gene
ethyl and 5-hydrox oxymethylcytosine Bsn I ,BspAN I, Bs
nce GTPy↓Pu*AC a
y 6-methyladenine methylcytosine at t Isoschizomer: Hinc nce *A↓°AG*CTT a hibited by 6-methy ne also inhibits. 6-rs: none known.
nce G↓*ANT°C and
nsitive to 6-methy position (°) does no ne does. Isoschizo nce GTT↓*A°AC an y 6-methyladenine hylcytosine does n
nce GGTAC↓C and
pn I is not inhibited 6-methyladenine. I are-cutter enzymes DNA. Recognizes with 3'-cohesive en soschizomers: Sac
nce C↓TAG and ge
pplied with the sp e concerning meth
nce A↓*CGT and g pplied with the sp nce of 5-methylcyto 4 IV. C↓GCT and genera the presence of 5-hibiting.Isoschizom TT*C and generate ed by 6-methylade sine as indicated (* nd generates fragm 6-methyladenine, a 5-methylcytosine ( Eco 32I. 13 and generates f which cleaves dsD y 6-methyladenine erating fragments xymethylcytosine a at the external C° uR I, Pho I. and generates frag e as indicated (*). H the 3’-C residue of c II. nd generates fragm yladenine or 5-met methyladenine at d generates fragme ladenine (*). Prese ot prevent cleavag mers: none known nd generates fragm e and 5-hydroxyme ot influence the cl d generates fragme d by 5-methylcytos Isoschizomers:Asp s which generate l the sequence CCG ds. Not sensitive to II, Sst II. enerates fragments ecial 2x incubation ylation sensitivity. enerates fragment ecial 2x incubation osine as indicated ates fragments -methylcytosine mers: Afe I, es fragments enine at either *). Eco RI is an ments with as indicated (°) or fragments with DNA at precise e as shown (*). with blunt at the internal °. gments with Hind II is f the ments with thylcytosine (*). A does not ents with ence of e, but n. ments with ethylcytosine leavage (°). ents with sine at either or p718 II, Acc65 I. large GC↓GG and o the presence s with n buffer. No Isoschizomers: ts with n buffer. (*). 11 167 103 001 10 200 310 001 10 606 189 001 10 703 737 001 11 175 084 001 10 667 145 001 10 667 153 001 11 040 197 001 11 004 816 001 10 693 944 001 10 656 305 001 10 656 313 001 10 656 321 001 10 798 983 001 11 274 040 001 10 779 652 001 10 779 679 001 11 274 082 001 10 380 385 001 10 567 647 001 10 742 953 001 10 899 186 001 11 117 807 001 10 822 221 001 10 862 495 001 2 100 U (5 U/µl) 10,000 U (40 U/µl) 50,000 U (40 U/µl) 5,000 U (10 U/µl) 10,000 U (10 U/µl) 2,000 U (10 U/µl) 10,000 U (10 U/µl) 10,000 U (40 U/µl) 100 U (1 - 5 U/µl) 5,000 U (10 U/µl) 2,500 U (3 - 10 U/µ 5,000 U (10 U/µl) 10,000 U (10 U/µl) 10,000 U (40 U/µl) 50,000 U (40 U/µl) 1,000 U (10 U/µl) 5,000 U (10 U/µl) 20,000 U (40 U/µl) 100 U (3 - 10 U/µl 500 U (3 - 10 U/µl 10,000 U (40 U/µl) 5,000 U (10 U/µl) 1,000 U (10 U/µl) 250 U (1 - 5 U/µl) 50 U (1 - 5 U/µl) ) ) ) ) ) ) µl) ) ) ) ) ) ) ) ) 185,20 51,00 205,20 41,00 51,00 73,20 275,10 275,10 125,40 132,90 463,70 54,40 96,40 96,40 350,20 67,70 268,40 794,20 73,20 292,80 190,80 118,70 92,10 360,50 339,40
Mae Reco 5´-co Mae none Mlu Reco 5´-co sequ 6-me MluN Reco blunt indic Mro Reco 5'-co no in Aor13 Mun Mun 5´-co recog Mva Reco 5´-co resid 6-me Mvn Mvn blunt sequ BstFN Nar I Reco 5´-co 4-me posit Nco Reco 5´-co Prese Isosc Nde Nde with recog Isosc Nde Reco 5´-co show Bsp1 Nhe Nhe 5´-co recog Not I Not I GC↓G by 5-not in Nru I Reco ends 5-me posit e III
ognizes the sequen ohesive termini. Su III is not known to e known.
I
ognizes the sequen ohesive termini. Inh ence as indicated ethyladenine (°). Is
N I (Bal I)
ognizes the sequen t ends. MluN I is in ated (*). Isoschizo
I (Acc III)
ognizes the sequen ohesive ends. Inhib hibition by overlap 3H I, BseA I, Bsp13 n I (Mfe I) I recognizes the s ohesive termini.Mu gnition sequence a I (BstN I) ognizes sequence * ohesive termini. Se ues, and 4-methyl ethyladenine. Isosc I (FnuD II) I recognizes the se t ends. Mvn I is inh ence, as indicated N I, BstU I. I
ognizes the sequen ohesive ends. Inhib ethylcytosine at the ions. Isoschizomer
I
ognizes the sequen ohesive termini. Inh ence of 6-methylad chizomers: Bsp19 I I I recognizes the se 5´-cohesive termin gnition sequence a chizomers: FauND II (Mbo I)
ognizes the sequen ohesive termini. Inh wn (*). No inhibition 43 I, BssM I, BstM I I recognizes the se ohesive termini. Nh gnition sequence a I is a rare-cutter en GG*C*CG°C, gener -methylcytosine as nhibiting (°). Isosch I
ognizes the sequen . Inhibited by over ethylcytosine is inh ion of the C. Isosc
nce ↓GTNAC and g
pplied with the sp o be inhibited by m
nce °A↓*CGCGT an
hibited by 5-methy (*). It is not influen oschizomers: none
nce TGG↓C*CA and
nhibited by overlap mers: Mls I, Bal I, M nce T↓*CCGG°A, g bited by 5-methylcy pping dam-methyla 3 I, BspE I, Kpn2 I. sequence C↓A*ATT un I is inhibited by as indicated. Isosc *C*C↓(A,T) GG, ge nsitive to simultan cytosine at extena chizomers: BstN I, equence *CG↓CG hibited by 5-methy (*).Isoschizomers nce GG↓*CGCC an bited by 5-methylcy e 3'-position and b rs: Mly113 I. nce *C↓C°ATGG an hibited by 5-methy denine does not in .
equence °CA↓T*AT
ni. Nde I is inhibite as indicated (*), bu
I.
nce ↓G*ATC, gener hibited by overlapp n by 5-methylcytos MB I, Dpn II, Kzo9 I, equence G↓CTAG* he I is inhibited by as indicated (*). Iso nzyme and recogn rating fragments w s shown (*). 5-meth hizomers: CciN I. nce T*CG↓°CG*A, g lapping dam-meth hibiting(*) or not in hizomers: BtuM I, generates fragmen ecial 2x incubation methylation. Isoschi nd generates fragm ylcytosine within th
nced by the presen e known. d generates fragm pping dcm-methyla Msc I, Msp20 I. enerating fragmen ytosine(*). In contr ation(°). Isoschizom TG and generates 6-methyladenine i hizomers: Mfe I. enerating fragment neous 5-methylatio al C. Inhibited by
BseBI, BstOI, Bst2U and generates fra ylcytosine within th : Acc II, Bsh1236 I, nd generates fragm ytosine as shown ( by 5'-hydroxymethy nd generates fragm ylcytosine as indica nfluence the cleava
TG and generates ed by 6-methyladen ut not by 5-methylc rating fragments w ping dam-methylat sine. Isoschizomer Mbo I, Sau3A I. *C and generates f 5'-methylcytosine oschizomers: AsuN izes the sequence with 5’-cohesive te hylcytosine in the 5 generating fragme hylation as shown( hibiting(°), depend Bsp68 I. nts with n buffer. izomers: ments with he recognition nce of ments with ation as nts with rast to BseA I,
mers: Acc III,
fragments with in the ts with on at both C UI. gments with he recognition , BspFN I, ments with (*). Inhibited by ylcytosine in all ments with ated (*). age (°). fragments nine within the cytosine (°). with tion as rs: BfuC I, fragments with within the NH I. rmini. Inhibited 5'-C position is
ents with blunt (*). ding on the 10 822 230 001 10 822 248 001 10 909 700 001 10 909 718 001 11 526 430 001 11 102 982 001 11 441 337 001 11 288 075 001 11 062 573 001 11 103 024 001 10 835 315 001 10 835 323 001 11 047 698 001 11 040 219 001 11 040 227 001 11 040 243 001 10 885 843 001 10 885 851 001 10 885 860 001 11 014 706 001 11 014 714 001 11 037 668 001 10 776 777 001 50 U (1 - 5 U/µl) 250 U (1 - 5 Uµl) 500 U (10 U/µl) 2,500 U (10 U/µl) 200 U (10 U/µl) 100 U (1 - 5 U/l) 200 U (10 U/µl) 5,000 U (10 U/µl) 200 U (10 U/µl) 1,000 U (10 U/µl) 200 U (10 U/µl) 1,000 U (10 U/µl) 1,000 U (40 U/µl) 200 U (10 U/µl) 1,000 U (10 U/µl) 1,000 U (5 U/µl) 200 U (10 U/µl) 1,000 U (10 U/µl) 1,500 U (40 U/µl) 200 U (10 U/µl) 1,000 U (10 U/µl) 1,000 U (40 U/µl) 1,000 U (10 U/µl) 88,70 360,50 67,70 234,00 104,20 344,90 168,60 198,60 106,40 245,00 102,00 383,80 383,80 84,30 336,10 307,20 71,00 283,90 337,20 88,70 342,80 342,80 355,50
Nsi I Nsi I 3’-co 5-me Zsp2 Pst I Pst I 3´-co and 6 Pvu Reco 3´-co Inhib Isosc Pvu Pvu I blunt recog Rsa Reco ends howe inhib Sac Reco 3’-co 5-me 6-me Sal I Sal I 5´-co N6-m Isosc Sau3 Reco termi 5- an Isosc Sca Sca I blunt indic SexA SexA with (*). Is Sfi I Analy GG°C Inhib Cs is Sfu I Reco 5´-co insen BspT Sma Reco Not i other Neos SnaB Reco blunt TAmC sequ
recognizes the seq ohesive termini. Ns ethylcytosine is not
I.
recognizes the seq ohesive termini. Pst 6-methyladenine, a
I
ognizes the sequen ohesive termini. No bited by 5-methylcy chizomers: BpvU I,
II
I recognizes the se t ends. Pvu II is inh gnition sequence a
I
ognizes the sequen . Rsa I is not inhib ever, the presence
iting as indicated
I (Sst I)
ognizes the sequen ohesive termini. Inh ethylcytosine at the ethyladenine (°). Is
recognizes the seq ohesive termini. Sa methyladenine with chizomers: none kn 3A I ognizes sequence ↓ ini. No inhibition b nd 4-methylcytosin chizomers: Mbo I, N I recognizes the se t ends. Sca I is not ated (°). Isoschizo
A I
AI recognizes the se 5'-cohesive termin soschizomers: Mab yze and clone larg
C*CNNNN↓NGG°C
bited by 5-methylcy not inhibiting (°).
I (Asu II)
ognizes the sequen ohesive termini. Inh nsitive to 5-methylc T104 I, BstB I, Csp4 I ognizes sequence*C nhibited by 5-meth r positions or 4-me schizomers: Cfr9 I, B I
ognizes the sequen t ends. Inhibited by CGTA. Inhibited by ence TmACGTmA quence ATGC*A↓T si I is inhibited by 6 t inhibiting. Isosch quence *CTGC°A↓ t I is inhibited by th as indicated (*). Iso nce CG°AT↓*CG, g ot inhibited by over ytosine as shown ( Mvr I, Ple19 I. equence CAG↓*CT hibited by 5'- and 4 as indicated (*). Iso nce GT↓*A°C and g ited by the presen
of 6-methyladenin (*). Isoschizomers:
nce G°AG*CT↓C an
hibited by 5-methy e other C does not oschizomers: Psp1
quence G↓T*CG*A
l I is inhibited by 5 hin the recognition nown.
↓G°AT*C. Generate
by dam gene which ne, 5-hydroxymethy Nde II, Dpn II and
equence AGT↓A°C
inhibited by the p mers: Ass I, BmcA equence A↓C*C(T ni. SexA I is dcm m b I. e DNA fragments. C°C. Generates frag ytosine at central C Isoschizomers: no nce TT↓°CG*AA an hibited by 6-methy cytosine (°). Isosch 45 I, Nsp V. C°C*C↓GGG. Gene hylcytosine at mid ethylcytosine in eit TspM I, Xma I, Xm nce T*A*C↓GT*A a
y 5´-methylcytosin y N6-methyladenin (*). Isoschizomers T and generates fr 6-methyladenine, a izomers: EcoT22 I, G and generates f he presence of 5-m oschizomers: BspM enerating fragmen rlapping dam-met (*) and 4-methylcy TG and generates f 4'-methylcytosine w oschizomers: none generates fragmen ce of 5-methylcyto ne or 4-methylcyto : Afa I. nd generates fragm ylcytosine at the ce
t inhibit, nor does 124B I, Sst I. AC and generates f 5-methylcytosine a n sequence as indi es fragments with h methylates N6 of ylcytosine inhibit a others. CT and generates fr presence of 5-meth A I, Zrm I. T,A)GGT and gener methylation sensitiv
. Recognizes the se gments with 3´-co C (*). 5-methylcyto one. nd generates fragm yladenine as indica hizomers: Bpu14 I, erates fragments w dle C(°). 5-methylc ther position inhibi maC I.
nd generates fragm e within the seque ne at either A with s: BstSN I, Eco105 ragments with as indicated (*). Mph1103 I, fragments with methylcytosine MA I. nts with hylation (°). ytosine. fragments with within the e known. nts with blunt osine (°), osine is ments with entral C (*). fragments with and cated (*). 5´-cohesive f adenine (°). at C(*). ragments with hylcytosine, as rates fragments ve as indicated equence hesive termini. osine at other ments with ated (*), but is Bsp119 I,
with blunt ends. cytosine at its(*). ments with ence in the I. 10 909 831 001 10 909 840 001 10 621 625 001 10 621 633 001 10 798 991 001 10 650 129 001 10 650 137 001 10 642 690 001 10 642 703 001 10 729 124 001 10 729 132 001 11 047 671 001 10 669 792 001 10 669 806 001 11 047 655 001 10 348 783 001 10 567 663 001 11 047 612 001 10 709 751 001 10 775 266 001 11 497 995 001 11 288 024 001 11 288 059 001 11 243 497 001 10 220 566 001 10 656 348 001 11 047 639 001 10 997 480 001 200 U (10 U/µl) 1,000 U (10 U/µl) 3,000 U (10 U/µl) 10,000 U (10 U/µl) 10,000 U (40 U/µl) 500 U (5 U/µl) 100 U (5 U/µl) 1,000 U (10 U/µl) 5,000 U (10 U/µl) 1,000 U (10 U/µl) 5,000 U (10 U/µl) 5,000 U (40 U/µl) 1,000 U (10 U/µl) 5,000 U (10 U/µl) 5,000 U (40 U/µl) 500 U (10 U/µl) 2,500 U (10 U/µl) 2,500 U (40 U/µl) 500 U (1 - 5 U/µl) 2,500 U (10 U/µl) 200 U (10 U/µl) 1,250 U (10 U/µl) 5,000 U (40 U/µl) 2,000 U (10 U/µl) 1,000 U (10 U/µl) 5,000 U (10 U/µl) 5,000 U (40 U/µl) 200 U (10 U/µl) ) ) ) 95,40 262,80 88,70 219,70 219,70 323,80 82,10 49,90 208,60 71,00 283,90 283,90 88,70 331,70 331,70 52,10 198,60 198,60 180,90 255,50 148,60 235,00 773,10 100,90 102,00 356,10 356,10 168,60
Spe Reco 5'-co N6-m Isosc Sph Reco 3´-co 5-me 5-hyd Ssp Ssp I blunt as ind Stu I Stu I blunt indic Swa Swa with geno Isosc Taq I Taq l 5'-co indic Tru9 Tru9 5´-co availa Xba Reco termi gene 5-hyd Xho Xho I with 6-me PaeR
SuR
Prod SuRE 10x o selec Tris-a 5 mM SuRE 10x o deter doub 10 m SuRE 10x o deter doub 10 m SuRE 10x o deter doub pH 7. Iognizes the sequen ohesive termini. Sp methyladenine and chizomers: Bcu I, A
I
ognizes the sequen ohesive termini. Inh ethylcytosine at
3'-droxymethylcytosin
I
recognizes the se t ends. The enzyme dicated (*). Isosch
I
recognizes the seq t ends. Stu I is inhi
ated (*). Isoschizo
I
I recognizes the se blunt ends. The en omes. No informati chizomers: Smi I.
I
recognizes the se ohesive termini. Taq
ated (*). Isoschizo I I recognizes the se ohesive termini. No able. Isoschizomer I ognizes sequence T ini. Requires at lea which methylates droxymethylcytosin I I recognizes the se 5’-cohesive termin ethyladenine and 5 R7 I, Sfr274 I, Sla I,
RE/Cut Buffer
duct Name E/Cut Buffer A optimized incubatio ct 100% activity or acetate, 660 mM p M dithiothreitol, pH E/Cut Buffer B optimized incubatio rmined to select 10 ble-digests. 100 mM M 2-mercaptoetha E/Cut Buffer H optimized incubatio rmined to select 10 ble-digests. 500 mM M dithioerythritol, E/Cut Buffer L optimized incubatio rmined to select 10 ble-digests. 100 mM .5 (at +37°C). nce *A↓*CTAGT an e I is inhibited by t 5’-methylcytosine Ahl I. nce GC*ATG?°C. G hibited by 6-methy C(°) or simultaneo ne at both Cs. Isosequence AAT↓*ATT
e is inhibited by th izomers: none kno
quence AGG↓*C*C
bited by the prese mers: Aat I, Eco14
equence ATTT↓AA
nzyme is very usefu on is available con equence T↓CG*A a q I is inhibited by o mers: Tth HB8 I. equence T↓TAA a o information conc rs: Mse I, Tru1 I. T↓*CTAGA. Genera
ast two nucleotide s 6N of adenine inh ne inhibit at (*). Iso
equence *C↓T*CG* ni. Xho I is inhibited 5-methylcytosine a Str I, Tli I.
rs
on buffer for RE di to calculate activit potassium-acetate, H 7.9 (at +37°C).on buffer for restri 00% activity or to c M Tris-HCl, 1 M Na anol, pH 8.0 (at +3 on buffer for restri 00% activity or to c M Tris-HCl, 1 M Na pH 7.5 (at +37°C) on buffer for restri 00% activity or to c M Tris-HCl, 100 mM nd generates fragm the presence of e ( mA?m CTAGT) enerates fragment yladenine(*). No in ous presence of schizomers: Bbu I, T and generates fr he presence of 6-m own. CT and generates f ence of 5-methylcy 7 I, Pce I, SseB I.
AAT and generates ul for digesting GC ncerning methylati
and generates frag overlapping dam-m
nd generates frag cerning methylation
ates fragments wit es around target se hibits; 5-methylcyt oschizomers: none
*AG and generates d by the presence as indicated (*). Iso
igests. Activity dete ty in double-digest , 100 mM magnesi
cition digests. Act calculate activity in aCl, 50 mM MgCl2
37°C).
cition digests. Act calculate activity in aCl, 100 mM MgC ).
cition digests. Act calculate activity in M MgCl2 , 10 mM ments with as shown (*) . ts with hibition by Pae I ragments with methyladenine fragments with ytosine as s fragments C-rich bacterial on sensitivity. gments with methylation, as ments with n sensitivity is th 5´-cohesive equence; dam tosine and e. s fragments of oschizomers: ermined to ts. 330 mM ium acetate, tivity n 2 , tivity n Cl2 , tivity n dithioerythritol, 11 008 943 001 11 008 951 001 11 207 644 001 10 606 120 001 11 026 534 001 11 026 542 001 11 026 950 001 10 972 975 001 10 753 351 001 11 047 680 001 11 371 517 001 10 567 671 001 11 175 114 001 11 464 825 001 10 674 257 001 10 674 265 001 10 674 273 001 11 047 663 001 10 703 770 001 10 703 788 001 10 899 194 001 Cat. No. 11 417 959 001 11 417 967 001 11 417 991 001 11 417 975 001 200 U (10 U/µl) 1,000 U (10 U/µl) 1,000 U (40 U/µl) 500 U (10 U/µl) 2,500 U (10 U/µl) 2,500 U (40 U/µl) 200 U (10 U/µl) 1,000 U (10 U/µl) 500 U (10 U/µl) 2,500 U (40 U/µl) 200 U (10 U/µl) 2,500 U (10 U/µl) 10,000 U (10 U/µl) 1,000 U (10 U/µl) 1,000 U (10 U/µl) 5,000 U (10 U/µl) 20,000 U (10 U/µl) 20,000 U (40 U/µl) 2,500 U (40 U/µl) 12,500 U (40 U/µl) 5,000 U (10 U/µl) Pack Size 5 x 1 ml 5 x 1 ml 5 x 1 ml 5 x 1 ml ) ) ) ) 88,70 342,80 342,80 261,70 908,50 908,50 118,70 262,80 76,60 307,20 100,90 102,00 301,60 245,00 44,40 103,20 390,40 390,40 52,10 210,70 99,80 Price in € 25,20 25,20 25,20 25,20
SuRE 10x o deter doub 10 m SuRE One s and H been activi
Clo
Prod rAPid Rapid inact requi deph Alka Calf I phos cloni Highe Alka Use t phos enzym conc Isopr Isopr lac o analo Meg Very large episo chrom Rapi Fast, or blu fragm linea Rapi Cova plasm recirc just 5 T4 D Use T Catal 3'-hy stran pBR3 Selec cleav sites inact pUC Plasm orien many E. co Stora Box w stora E/Cut Buffer M optimized incubatio rmined to select 10 ble-digests. 100 mM M dithioerythritol,E/Cut Buffer Set
set containing 1 m H for DNA restricti determined in eac ity in double-diges
ning of DNA
duct Name d Alkaline Phosp d, efficient dephos ivated at +75°C fo ired after restrictio osphorylated DNAaline Phosphatase
Intestinal Alkaline phates from DNA ng efficiency by pr er conc. 20 U/µl en
aline Phosphatase
this calf intestinal a phates from DNA me (20 U/µl), conv entration (1 U/µl) ropyl-ß-D-thioga ropyl-β-D-thiogal operon. It binds an og of galactose tha ganuclease I-Sce rare-cutter endon e DNA fragments, a
omal DNA, cloned mosomal DNA isol
d DNA Dephos &
efficient dephosph unt-end DNA frag ments into plasmid r DNA, and library
d DNA Ligation K
lently joins sticky-mid or phage vecto cularizing linear D 5 minutes at room
DNA Ligase
T4 DNA ligase to jo lyzes formation of ydroxyl- and 5'-pho d nicks in dsDNA
322 DNA
ctable plasmid for vage sites in the am
in the tetracycline ivating these drug
18 DNA
mid for loning doub ntation with respec
y unique sites for c
li by transformatio
age Box
with removable sty ge of 50 microcen
on buffer for restri 00% activity or to c M Tris-HCl, 500 mM
pH 7.5 (at +37°C)
for Restriction E
ml 10x solutions ea ion digests. Activit ch buffer to select sts.
Fragments
phatase sphorylation of 5’ e or 2 minutes. No ad on and dephospho A directly in ligatio e Phosphatase (1 U or RNA in small-s reventing self ligat nzyme is also avaie
alkaline phosphata or RNA. This prep venient for large-sc
is also available. alactoside actoside (IPTG) is d inactivates the la at cannot be cleave e I uclease. Use in ad and for mapping b mammalian DNA lated from
pulsed-& Ligation Kit
horylation (10 min ments. Minimizes d and phage vector y generation.
Kit
- or blunt-end DNA ors, adding linkers NA. Contains all re
temperature. oin sticky- or blun phosphodiester bo osphate ends in do are also closed by cloning of recomb mpicillin gene, or C e gene, allow foreig resistance genes. ble-digested restri ct to the lac promo
cloning of foreign on.
yrofoam insert (0.5 ntrifuge tubes. Tem
cition digests. Act calculate activity in M NaCl, 100 mM M ). nzymes ch of SuRE/Cut Bu ty of all restriction t 100% activity or to
ends of DNA and R dditional purificatio rylation. Use the n reactions. /µl) removes 5'-te scale experiments, tion of linearized v
lable.
ase to remove 5'-te p has a high conce
cale experiments. A
a very effective in
ac repressor. It is
ed by β-galactosi dapter cloning and bacterial or yeast c fragments, or mam -field gels. ) and ligation (5 m application times w rs, linker ligation, r
A fragments for clo to plasmids or oth eagents needed fo
t-ended DNA frag onds between neig ouble-stranded DN y T4 DNA Ligase. binant DNA. Pst I a Cla I, Hind III, Bam gn DNA to be inse .
iction fragments in oter. Polylinker (MC
DNA. Easily introd
to 2.0 ml capacity mperature-resistant tivity n MgCl2 , uffer A, B, L, M, enzymes has o calculate RNA. Rapidly on steps rminal enhancing vector DNA. erminal entration of A lower ducer of the a chemical idase. subcloning of chromosomes, mmalian min) of sticky- when cloning recircularizing oning into her DNAs, and or ligation in
gments. ghboring NA.
Single-and Pvu I HI, and Sal I erted for n either CS) provides duced into y) for the t to -70°C. 11 417 983 001 11 082 035 001 Cat. No. 04 898 133 001 04 898 141 001 10 713 023 001 11 097 075 001 10 724 815 001 11 411 446 001 11 362 399 001 04 898 117 001 04 898 125 001 11 635 379 001 10 481 220 001 10 716 359 001 10 799 009 001 10 481 238 001 10 885 819 001 10 800 058 001 1 kit 1 kit 1 kit for up 5 x 1 ml 1 set Pack Size 1,000 U (1 U/µl) 5,000 U (1 U/µl) 1,000 U (1 U/µl) 1,000 U (20 U/µl) 1 g 5 g 1,000 U t for up to 40 react for up to 160 reac p to 40 DNA ligatio 100 U (1 U/µl) 500 U (1 U/µl) 500 U (5 U/µl) 200 µl 1 A260 unit 50 µg (200 µl) 1 box tions ctions on reactions t 25,20 22,80 Price in € 88,50 354,90 86,50 100,90 80,20 300,50 404,80 170,30 487,30 279,10 85,10 268,80 268,80 207,10 456,90 7,20
Mo
Prod DNA For s mole gene 23.1 DNA DNA in So label DNA For s deter restri DNA DNA in So label DNA For s deter restri DNA For s deter restri DNA For s deter restri DNA DNA stand acid DNA For s deter restri DNA DNA stand acid DNA For s deter restri DNA DNA stand acid DNA For s deter restri DNA For s deter restri DNA For s deter restri DNA For s deter restri additlecular Weigh
duct Name A Molecular Weig ize distribution an cular weight deter rated by restriction kbp.A Molecular Weig
Molecular Weight outhern blot analys ing and detection.
A Molecular Weig ize distribution in rmination of doubl iction digests, PCR A Molecular Weig Molecular Weight outhern blot analys ing and detection.
A Molecular Weig ize distribution in rmination of doubl iction digests, PCR A Molecular Weig ize distribution in rmination of doubl iction digests, PCR A Molecular Weig ize distribution in rmination of doubl iction digests, PCR A Molecular Weig Molecular Weight dard in Southern b labeling and detec
A Molecular Weig ize distribution in rmination of doubl iction digests, PCR A Molecular Weig Molecular Weight dard in Southern b labeling and detec
A Molecular Weig ize distribution in rmination of doubl iction digests, PCR A Molecular Weig Molecular Weight dard in Southern b labeling and detec
A Molecular Weig ize distribution in rmination of doubl iction digests, PCR A Molecular Weig ize distribution in rmination of doubl iction digests, PCR A Molecular Weig ize distribution in rmination of doubl iction digests, PCR A Molecular Weig ize distribution in rmination of doubl iction digests, PCR tional band of 2642
ht Markers
ght Marker IIalysis using agaro rmination of doubl n digests, PCR, an
ght Marker II, DIG
t Marker II, DIG-la sis when using the Size range: 0.12 to
ght Marker III
agarose gels. Simp e-stranded DNA f R, and RT-PCR. Size
ght Marker III, DI
t Marker III, DIG-la sis when using the
Size range: 0.12 to
ght Marker IV
agarose gels. Simp e-stranded DNA f R, and RT-PCR. Size
ght Marker V
agarose gels. Simp e-stranded DNA f R, and RT-PCR. Size
ght Marker VI
agarose gels. Simp e-stranded DNA f R, and RT-PCR. Size
ght Marker VI, DI
t Marker VI, DIG-la blot analysis when
ction.Size range: 0.
ght Marker VII
agarose gels. Simp e-stranded DNA f R, and RT-PCR. Size
ght Marker VII, D
t Marker VII, DIG-l blot analysis when ction.Size range: 0.
ght Marker VIII
agarose gels. Simp e-stranded DNA f R, and RT-PCR. Size
ght Marker VIII, D
t Marker VII, DIG-l blot analysis when ction.Size range: 19
ght Marker IX
agarose gels. Simp e-stranded DNA f R, and RT-PCR. Size
ght Marker X
agarose gels. Simp e-stranded DNA f R, and RT-PCR. Size
ght Marker XIII (5
agarose gels. Simp e-stranded DNA f R, and RT-PCR. Size
ght Marker XIV (1
agarose gels. Simp e-stranded DNA f R, and RT-PCR. Size 2 bp. se gels. Simplifies e-stranded DNA f d RT-PCR. Size ran G-labeled beled, is used as a DIG System for nu o 23.1 kbp. plifies accurate mo fragments generate e range: 0.12 to 21 IG-labeled abeled, is used as DIG System for nu o 21.2 kbp. plifies accurate mo fragments generate e range: 0.07 to 19 plifies accurate mo fragments generate e range: 8 to 587 b plifies accurate mo fragments generate e range: 0.15 to 2. IG-labeled abeled, is used as using the DIG Sys .15 to 2.1 kbp. plifies accurate mo fragments generate e range: 0.081 to 8 DIG-labeled labeled, is used as using the DIG Sys .081 to 8.57 kbp. plifies accurate mo fragments generate e range: 19 to 111 DIG-labeled labeled, is used as using the DIG Sys 9 - 1114 bp. plifies accurate mo fragments generate e range: 72 to 135 plifies accurate mo fragments generate e range: 0.075 to 1 50 bp ladder) plifies accurate mo fragments generate e range: 50 to 750 100 bp ladder) plifies accurate mo fragments generate e range: 100 to 15 accurate fragments nge: 0.12 to a size standard ucleic acid olecular weight ed by 1.2 kbp. a size standard ucleic acid olecular weight ed by 9.3 kbp. olecular weight ed by bp. olecular weight ed by 1 kbp. a size stem for nucleic
olecular weight ed by 8.57 kbp. s a size stem for nucleic
olecular weight ed by 4 bp. s a size stem for nucleic
olecular weight ed by 3 bp. olecular weight ed by 12.2 kbp. olecular weight ed by bp. olecular weight ed by 00 bp and an Cat. No. 10 236 250 001 11 218 590 910 10 528 552 001 11 218 603 910 11 418 009 001 10 821 705 001 11 062 590 001 11 218 611 910 11 209 264 001 11 669 940 910 11 336 045 001 11 449 451 910 11 449 460 001 11 498 037 001 11 721 925 001 11 721 933 001 50 µg in 200 50 50 µg in 200 50 50 µg in 200 50 µg in 200 50 µg in 200 50 50 µg in 200 50 50 µg in 200 500 50 µg in 200 100 µg in 400 50 µg (1 A 50 µg in 200 Pack Size µl (1 A260 unit) fo lanes 00 µl (5 µg, 10 µg/m µl (1 A260 unit) fo lanes 00 µl (5 µg, 10 µg/m µl (1 A260 unit) fo lanes µl (1 A260 unit) fo lanes µl (1 A260 unit) fo lanes 00 µl (5 µg, 10 µg/m µl (1 A260 unit) fo lanes 00 µl (5 µg, 10 µg/m µl (1 A260 unit) fo lanes 0 µl (10 µg/ml) (5 µl (1 A260 unit) fo lanes µl (2 A260 units) f lanes A260 unit) for up to 5 µl (1 A260 unit) fo lanes or up to 50 gel ml) or up to 50 gel ml) or up to 50 gel or up to 50 gel or up to 50 gel ml) or up to 50 gel ml) or up to 50 gel µg) or up to 50 gel for up to 50 gel 50 gel lanes or up to 50 gel Price in € 97,70 195,50 108,30 195,50 157,80 148,30 148,30 194,20 148,30 220,40 169,50 243,80 186,00 122,50 129,60 136,40
