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Investigating the Role and Structure of Free Radicals in Lignin Biosynthesis.

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Figure 2.1: Composition of the plant cell wall5.
Figure 1.2: The relative contributions of the three major monolignols to the lignin in the various layers and the cell wall-bound enzymes might participate in the various steps of lignification10
Figure 1.3: The chemical structures of three classical monolignols (Top left). The three building blocks of lignin (Top middle)
Figure 1.4: a) Structure of iron heme group in Horseradish Peroxidase23; b) Catalytic cycle of heme peroxidase22a; c) Structure of multi-copper cluster in laccase24; d) Monolignol oxidative dehydrogenation by HRP or laccase
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Theory of EPR Spectroscopy Introduction to spin-trapping technique Introduction Results and discussion of HRP-generated monolignol radicals Results and discussion of laccase-generated monolignol radicals Probe unpaired electron density from isotropic hyperfine parameters Preparation of UV-induced monolignol radical p Glucocoumaryl alcohol deglucosidation and oxidative dehydrogenation using β-

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