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Cell mixing during the early development of mouse aggregation chimera

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Figure

Fig. 1. Cell mixingin preimplantationgenotypeopedchimeras.(A,B,C,DJ. Immunofluorescencestainingof well developedC3H/HeNx C57BU6N(A)andC3H/HeNx BALB/cA (8) chimeric blastocysts24 h after aggregation.The stained cells are of the C3H genotypewhile negativecells are of the C57BLor BALBkgenotype.It is importantto notethatonlyone representativeoptical section is shown.When the blastocystswere optically sectionedandratios at each optical cut were determined,the total cellratiowasequal.UniformlydistributedC3H-speciflccellmarker - CSA in the con-trol (C3H/HeNx C3H/HeN)aggregatesICI confirmedspecificityof the system.Immunofluorescencestaining and morphologyof incorrectly devel-chimericblastocyst;in thiscasewith2 blastocoels(D), Suchaggregateswereexcludedfromtheseexperiments.
Fig. 2. Immunoperoxidasestainingtion. IAIof a C3H/HeN x C57Bl/6Nchimeraat the late primitivestreakstage.CD-1 fostermother.Saggitalsec- CSA positive cells (arrowhead)are located alongthe visceral embryonal endoderm
Fig. 3.Immunoperoxidasechimerapositive.stainingof a C3H/HeNx C57BL/6Nat the late primitivestreakstage.Co.7 foster mother is CSASaggital section.Positive C3H cells are sporadically distributedover the embryonalectoderm(e) and in the mesodermalcomponentofammon(a) but the extraembryonalecroplacentalcone is uniformly posi.tive (arrowhead).
Fig. 4. Immunoperoxidasestaininghead).mother. Cells of both genotypes are intermingleder C3H genotypeof a C3H/HeNxBAlB/cAchimeras.Late primitivestreak stage.Saggitalsection.(A) Bothgenotypesare intermingledin alt embryonalas well as extraembryonaltissues.N
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