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Analysis of steroid hormones and their conjugated forms in water and urine by on-line solid-phase extraction coupled to liquid chromatography tandem mass spectrometry

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Figure

Fig. 1 Chemical structures of target free and conjugated estrogens (drawn using ChemBioDra Ultra 14.0)
Fig. 2 The EQuan™ system (column-switching technique) schema used in this experiment
Table 1 Tandem mass spectrometry (MS/MS) optimized parameters for  the analysis of  selected estrogens hor-
Table 2 Recovery values in  percentage for  the selected estrogens using the SPE BetaBasic column in HPLC water samples
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