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The F9 EC cell line as a model for the analysis of differentiation

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Fig. 1. Morphologyof exponentiallygrowingF9 cells (>.200),
Fig. 2. G-bandedkaryotypeof F9 cells.ExponenriallygrowingF9 cellsweretreatedwithColcemid.spreadonglassslicesandstainedwirhGiemsastainafterTrypsintreatment.Typicalfor F9 cellsis the presenceof a metacentflcmarker chromosomeand an Insertionon chromosome4(arr
Fig. 3. Morphologycells.of in vitro differentiatedF9F9 cells were seededat an initial densityof600.000cel/sllOcmplateongelatinizedtissueculturedishes_CellsweretreatedeitherwithRAand dbcAMPfA) or withRA aloneat final concen-trationsof 10-and 10-3 respectively.The cells werepassagedeverysecondday duringthe differentia-tion time.DifA and DifB gavethe two differentiatedphenotypesappearingduringtreatmentwithRAas describedin the text (x 200).
Fig. 4.lmmunolocalizationof cytokeratinsand lamininduringdifferentiation.Cellswere treated for 3davswith RA anddbcAMP(A,BJor for 5 dayswith RA alone (C,D) using the culture conditions85 describedin Fig

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