Depletion Respiration

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130

The investigation and monitoring of biological cleaning treatment processes are becoming more and more important with respect to the environmental require-ments such as wastewater treatment, soil remediation and waste treatment. Biological tests are often in the fore-ground as well as the usual physical-chemical measuring methods. In order to determine the biodegradability of foodstuffs, pollutants, harmful substances or waste substances and the microbial activity so-called respiration (depletion) measurements are often carried out. In these measurements the respiration of the organisms is determined under defined conditions as the oxygen uptake or release of carbon dioxide.

Technical Data:

OxiTop®_{-C measuring head}

and OxiTop®_{Control OC 110} _{see page 120}

Stirrer platforms see page 124 and 135

Respiration

Literature

Depletion/Respiration

Modular system for

respiration measurements

Selected accessories

Favorable package price

Features

Soil respiration

Soil analysis:

biodegradation of contaminated sites

Biodegradation according to

OECD

Biogas determination

Anaerobic degradation

Microbiology

Growth and stress investigations: determination of respiration rate

Further information on this subject can be requested from WTW:

Application reports Offprints

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General Features Sample Preparation Laborator y & Field Respiration BOD Multi-Parameter Con-ductivity Oxygen (D.O.) ISE ORP pH Colony _Counter Photo- metr y T urbidity Software, Printers Customer Ser vice

Depletion/Respiration

Laboratory method for determining the

microbial soil respiration according to

DIN 19 737

Soil respiration measurements are used for forecasting, surveying and checking remediation work, for biodegradability measurements of substances (pesticides, fungicides, fertilizers, etc.) as well as for carrying out toxicity tests.

This determination is possible with the OxiTop®_{Control System} and special, practically-tested measuring vessels in a very accurate, simple and favorably-priced way.

The expenditure for personnel and apparatus is considerably

reduced when compared with conventional methods. Soil respiration measurements can be carried out in 2 different types of vessel.

For actively respiring soils with strong CO2development the

MG 1.0 measuring vessel is recommended: its large opening (approx. 100 mm dia.) means that it can be easily used with large-volume CO2absorber vessels for quantitative CO2

determination.

Example of application using PF/45... sample vessels

Model Complete soil respiration package Order No. OxiTop®_{Control B6M} _{Package for soil respiration (aerobic) with} _{208 232}

6 MG 1.0 measuring vessels, 1000 ml, with stopper adapters for OxiTop®_-C

OxiTop®_{Control B6} _{Package for soil respiration (aerobic) with} _{208 230}

6 PF 45/500 sample vessels, 500 ml, Duran and 6 OxiTop®_{AD/SK adapters, autoclavable}

Ordering Information for soil respiration

Determination of soil respiration

For the determination of aerobic soil respiration optimized application-oriented complete packages are available.

Equipment

6 OxiTop®_-C_{measuring heads}

6 MG 1,0 measuring vessels, 1000 ml with stopper adapters for OxiTop®_-C

alternatively:

6 PF 45/500 sample bottles, 500 ml, Duran, with 6 OxiTop®_{AD/SK adapters, autoclavable}

1 Controller OC 110with operating instructions 1 PC communication program Achat OCfor further data

processing and documentation of the measuring data 1 Interface cable AK 540 B

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132

Model Complete OECD packages Order No. OxiTop®_{Control A6} _{Package for aerobic applications with 6 x 1000 ml measuring places} _{208 220}

OxiTop®_{Control A12} _{Package for aerobic applications with 12 x 250 ml measuring places} _{208 222}

OxiTop®_{Control S6} _{Package for aerobic applications with 6 x 510 ml measuring places} _{208 196}

OxiTop®_{Control S12} _{Package for aerobic applications with 12 x 510 ml measuring places} _{208 198}

Note: versions for 120 VAC/50/60 Hz on request

Ordering Information for OECD

The determination of the biodegradability should be checked before “new” chemicals are used for the first time, not only for environmental reasons but also to minimize disposal charges. The sample prepared according to the instructions and a blank are stirred at a constant temperature for 28 days in closed bottles.

The CO2produced is removed from the gas space by means

of an absorber so that the resulting negative pressure is a mea-sure of the biodegradability.

The continuous recording of the values in the OxiTop®_-C

means that the required documentation can be guaranteed in an optimal manner.

The measuring bottles and adapters can be autoclaved at 121 °C.

Determination of biodegradability according to

DIN EN 29 408 / ISO 9408 / OECD 301 F

1 Controller OC 110 with operating instructions 1 PC communication program Achat OC 1 Interface cable AK 540/B

1 IS 6-Var stirrer platform IS 12 stirrer platform

with operating instructions with operating instructions and 230 V plug-in and 230 V plug-in

power supply power supply 1 NHP 600 (1 bottle of NaOH pellets) 1 NTH 600 Nitrification inhibitor 1 REF 600 Stirrer bar remover

Equipment per Equipment per

A6 package: A12 package:

1 OxiTop®_{-C measuring head}

1 Measuring bottle Measuring bottle PF45/1000 PF45/250

1 OxiTop®_{AD/SK adapter}

1 RST 600 Stirrer bar

Equipment per Equipment per

A6 measuring place: A12 measuring place:

If BOD sample bottles

are used:

1 IS 6 stirrer platform IS 12 stirrer platform

Equipment per Equipment per

S6 package: S12 package:

1 Measuring bottle Measuring bottle PF600 PF600

1 GK 600 L rubber sleeve

Equipment per Equipment per

S6 measuring place: S12 measuring place:

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Depletion/Respiration

Model Complete packages for biogas determination Order No. OxiTop®_{Control AN6} _{Package for aerobic or anaerobic} _{208 225}

applications with 6 x 1000 ml measuring places

OxiTop®_{Control AN12} _{Package for aerobic or anaerobic} _{208 227}

applications with 12 x 250 ml measuring places

Ordering Information

Anaerobic degradation processes take place in the absence of oxygen. In order to be able to fill the gas space above the sample with inert gas the measuring bottle has side-mounted connections which are sealed with septa. When an-aerobic degradation has taken place the dissolved CO2can be driven off and

then removed from the gas space by

means of a CO2absorber. The resulting

pressure difference is proportional to the CO2concentration, the remaining

over-pressure is proportional to the methane concentration.

The degradation process can be com-fortably followed in the “pressure” operating mode.

Determination of anaerobic degradation processes:

biogas determination

Biogas determination

1 Controller OC 110 with operating instructions 1 PC communication program Achat OC 1 Interface cable AK 540/B 1 IS 12 stirrer platform with

operating instructions and 230 V plug-in power supply

1 NHP 600 (1 bottle of NaOH pellets)

1 NTH 600 Nitrification inhibitor 1 REF 600 Stirrer bar remover

Equipment per

AN12 package:

1 Measuring bottle MF45/250

Equipment per

AN12 measuring place:

1 Controller OC 110 with

operating instructions 1 PC communication program

Achat OC

1 Interface cable AK 540/B 1 IS 6-Var stirrer platform with

operating instructions and 230 V plug-in power supply

1 NHP 600 (1 bottle of NaOH pellets)

1 NTH 600 Nitrification inhibitor 1 REF 600 Stirrer bar remover

Equipment per

AN6 package:

1 Measuring bottle MF45/1000

Equipment per

AN6 measuring place:

Complete packages for

biogas determination

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134 Microbiological growth and stress

investigations: determination of the

respiration rate

The use of special measuring bottles with side-mounted con-nections and septum closures allows the interference-free addition of substrates and solutions.

As pressure alterations could indicate a reduction in oxygen concentration, for example, which could make the addition of oxygen or air necessary (possibly other gases as well) it is possible to set a “warning pressure” or a pressure limit so that the operator can carry out manipulations on the system. The momentary pressure can be stored so that the manipula-tion is fully documented.

Model Complete packages for microbiology Order No. OxiTop®_{Control AN6} _{Package for aerobic or anaerobic applications with 6 x 1000 ml measuring places} _{208 225}

OxiTop®_{Control AN12} _{Package for aerobic or anaerobic applications with 12 x 250 ml measuring places} _{208 227}

Complete packages for aerobic measurements

Ordering Information for microbiology

1 Controller OC 110 with operating instructions

1 PC communication program Achat OC 1 Interface cable AK 540/B

Equipment per Equipment per

AN6 package: AN12 package:

1 Measuring bottle Measuring bottle MF45/1000 MF45/250

Equipment per AN6 Equipment per AN12

measuring place: measuring place:

Complete packages

for aerobic measurements

Equipment per Equipment per

A6 package: A12 package:

1 Measuring bottle Measuring bottle PF45/1000 PF45/250

Equipment per Equipment per

A6 measuring place: A12 measuring place:

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Depletion/Respiration

Model

Order No.

IS 6-Var Stirrer for 6 stirrer positions for sample bottles with a larger base diameter. 208 145 mains operation 230 V/50/60 Hz.

Note:versions for 120 VAC/50/60 Hz on request

Soil respiration accessories / Spare parts

MG 1.0 Measuring vessel MG 1.0, 1000 ml, complete for OxiTop®_{-C, consisting of} _{209 019}

1 x DV/MG, 4 x DV/MG/KL, 1 x MB50 and 2 x GK 600 L

MG 1.5 Measuring vessel MG 1.5, 1500 ml with stopper adapter for OxiTop®_{-C, and} _{209 021}

1 x DV/MG, 4 x DV/MG/KL, 1 x MB50 and 2 x GK 600 L

MG/ 1.0/E Measuring vessel MG 1.0, 1000 ml, minimum order 6 pieces 209 510

MG/ 1.5/E Measuring vessel MG 1.5, 1500 ml, minimum order 6 pieces 209 511

DV/MG Stopper adapter for measuring vessels MG 1.0 and MG 1.5 with support and seal 209 022

DV/MG/KL Clip for measuring vessels MG 1.0 and MG 1.5, 209 520 minimum order 20 pieces

DV/MG/DI Seal for measuring vessels MG 1.0 and MG 1.5, 209 521 minimum order 5 pieces

MB50 Beaker, 50 ml, as CO2absorber 108 064

Measuring and sample bottles

PF 45/100 Sample bottle, 100 ml, Duran, minimum order 3 pieces 209 101

MF 45/250 Measuring bottle 250 ml (Duran) with 2 connections; complete with accessories 209 106 2 x VS25, GK 600 L, 2 x SEP25, 2 x STH25

OxiTop®_AD/SK _{Adapter OxiTop}®_{-AD/SK, autoclavable (121 °C), consisting of} _{209 016}

1 x OxiTop®_{AD, 1 x OxiTop}®_{SK, 1 x DI45, 1 x VS45 and 2 x GK 600 L}

OxiTop®_AD _{Adapter, autoclavable (121 °C), with 1 x DI45} _{209 017}

OxiTop®_SK _{Screw-on sleeve, autoclavable (121 °C)} _{209 018}

DI 45 Seal for measuring vessels PF 45/... autoclavable (121 °C), 209 501 minimum order 5 pieces

SEP 25 Septum for measuring bottle MF45/..., minimum order 3 pieces 209 503

Ordering Information for accessories and spare parts

For BOD measurement with OxiTop

®

_{... see also BOD measurement section from page 114.}

IS 6-Var Stirrer platform

The IS 6-Var Stirrer platform has 6 stirrer positions on the base area of the IS 12 Stirrer platform. This means that sam-ple bottles with a larger base diameter, e.g. MF45/500, MF45/1000 or PF45/500 and PF45/1000 can be stirred. The technical specifications are otherwise the same as for the IS 12 Stirrer platform (see page 124).