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Curriculum Vitae

Dr. Daniel Tietze

B

USINESS

A

DDRESS

Technische Universität Darmstadt

Eduard‐Zintl‐Institute for Inorganic and Physical Chemistry

Alarich‐Weiss‐Str. 8 64287 Darmstadt Germany Phone: +49 6151 16 2796 Fax.: +49 6151 16 4347

P

ERSONAL

D

ATA

Date of birth: Place of birth: Nationality: Family status: Profession: Specialized in: 1980 Pößneck, Germany

German

married, one child

Chemist (Diploma)

Physical Chemistry (PhD)

E

DUCATION AND ACADEMIC CAREER

10/2009 11/2005‐10/2009 10/2004‐08/2005

PhD defense, summa cum laude

PhD in physical chemistry, Friedrich‐Schiller‐University, Institute of

Physical Chemistry, Jena, Germany,

Prof. Dr. G. Buntkowsky (now Technische Universität Darmstadt)

Title: “Investigations on the Mechanism of Nickel Superoxide Dismutase

Using Peptide Based Model Systems”

Diploma in chemistry, grade: excellent, Technical Chemistry and

Environmental Chemistry, Friedrich‐Schiller‐University Jena, Germany,

Prof. Dr. G. Kreisel, Dr. S. Meyer

Title: “Photooxidation von Citronellol in verschiedenen Reaktortypen“

(„Photo‐oxidation of citronellol in various reactor types“)

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P

ROFESSIONAL

E

XPERIENCE 08/2013‐current 07/2012‐07/2013 11/2009‐06/2012

Habilitand, Junior scientist & research group leader Eduard‐Zintl‐

Institute for Inorganic and Physical Chemistry, Technische Universität

Darmstadt, Prof. Dr. Gerd Buntkowsky

Project: “NMR as a tool to study structure and function of peptides and

ion channels”

PostDoc, Department of Chemistry, Iowa State University, Ames, USA,

Prof. Dr. M. Hong (now Massachusetts Institute of Technology ‐ MIT,

Cambridge, MA, USA)

Project: “Conformation and dynamics of the influenza A and influenza B

proton channel investigated by solid‐state NMR”

PostDoc, Eduard‐Zintl‐Institute for Inorganic and Physical Chemistry,

Technische Universität Darmstadt, Prof. Dr. Gerd Buntkowsky

S

CHOLARSHIPS AND

A

WARDS

2015 2014 2013 2011

Fonds der Chemischen Industrie research grant, project: “NMR structure

analysis, in‐silico ligand design, theoretical binding studies of novel

Gq/11 inhibitors.”

DAAD travel grant, 56th Rocky Mountain Conference on Magnetic

Resonance July 13th – 17th 2014, Copper Mountain, Colorado, USA

Travel grant, 3rd winter school on Biomolecular Solid‐State NMR, Jan.

6th ‐11th 2013, Stowe, Vermont, USA

Travel grant, SMARTER 2 workshop, May 23rd – 27th 2011, Aveiro,

Portugal

I

NVITED

L

ECTURES

1. invited talk: 12th German Peptide Symposium, March 18.‐21. 2015, Darmstadt, Germany

2. invited talk: “Pharmazeutisches Montagskolloqium der Pharmazie”, November 2013, Institute of

Pharmaceutical Biology, Rheinische Friedrich‐Wilhelms‐Universität Bonn, Bonn, Germany

3. invited talk: June 2013, Chemistry Department of Pharmaceutical Sciences and Experimental

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P

ARTICIPATION AT

I

NTERNATIONAL

C

ONFERENCES

1. talk: D. Tietze “Exploring enzyme function through model peptides: Conformation and Dynamics

studied by solid‐state NMR”, 12th German Peptide Symposium, March 18.‐21. 2015, Darmstadt,

Germany

2. poster: J. K. Williams, D. Tietze, J. Wang, Y. Wu, W. F. DeGrado, M. Hong “SSNMR Studies of the

Conformation, Dynamics and Small‐Molecule Interactions of Wild‐Type and S31N Mutant

Influenza M2 Proton Channels”, 56th Rocky Mountain Conference on Magnetic Resonance July

13. – 17. 2014, Copper Mountain, CO, USA

3. poster: D. Tietze, S. Voigt, M. Tischler, G. Buntkowsky “Revealing the position of the substrate in

NiSOD: a model study”, SMARTER 2 workshop, May 23. – 27. 2011, Aveiro, Portugal

4. poster: D. Tietze, S. Voigt, M. Tischler, D. Imhof, H. Breitzke, G. Buntkowsky “Investigations on

the Catalytic Mechanism of Nickel Superoxide Dismutase using solid‐state NMR” EUROMAR, July

4. – 9. 2010, Florence, Italy

5. talk: D. Tietze “Investigations on the Catalytic Mechanism of Nickel Superoxide Dismutase using

liquid and solid‐state NMR techniques” Ampere NMR School, June 20. – 26. 2010, Wierzba,

Poland

6. talk: D. Tietze “Investigations on the Catalytic Mechanism of Nickel Superoxide Dismutase”

Ampere NMR School, June 21. – 27. 2009, Zakopane, Poland

7. talk: D. Tietze “Investigations on the Catalytic Mechanism of Nickel Superoxide Dismutase” AK

Treffen Festkörper‐NMR, March 6. – 8. 2009, Heidelberg, Germany

8. poster: D. Tietze, D. Imhof, H. Breitzke, G. Buntkowsky “Mechanistic Investigations on NiSOD

Biomimetics Using Solid‐State NMR”, Ampere NMR School, June 19. – 28. 2008, Poznań/

Wierzba, Poland

C

OLLABORATIONS

(

MAJOR

)

 Prof. Mei Hong, PhD, Department of Chemistry, MIT ‐ Massachusetts Institute of Technology,

Cambridge, MA, USA

 Prof. Dr. Evi Kostenis, Institute of Biological Pharmacy, Rheinische Friedrich‐Wilhelms‐

Universität Bonn

 Prof. Dr. Gerd Buntkowsky, Eduard‐Zintl‐Institute for Physical and Inorganic Chemistry,

Technische Universität Darmstadt

 Prof. Dr. Doreen Mollenhauer, Theoretical Chemistry, Justus‐Liebig University, Gießen

 Prof. Dr. Diana Imhof, Institute of Pharmacy, Rheinische Friedrich‐Wilhelms‐Universität Bonn

 Prof. Dr. Gabriele M. König, Institute of Biological Pharmacy, Rheinische Friedrich‐Wilhelms‐

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 Prof. Dr. Thomas Prisner, Institute of Physical and Theoretical Chemistry, Goethe University

Frankfurt, Frankfurt, bio molecular EPR group

 Prof. Dr. Stefan H. Heinemann, Institute of Biochemistry and Biophysics, University of Jena,

electrophysiology group

 Prof. M. Ashley Spies, PhD, Department of Pharmaceutical Sciences and Experimental

Therapeutics, University of Iowa, Iowa City, IA, USA

 Prof. Dr. Harald Kolmar, Institute of Biochemistry, Technische Universität Darmstadt

 Prof. Dr. Gerhard Thiel, Department of Biology, Technische Universität Darmstadt

 Prof. Dr. Bodo Laube, Department of Biology, Technische Universität Darmstadt

 Dr. Indra Schröder, Department of Biology, Technische Universität Darmstadt

 Dr. A. A. Tietze, Institute of Biochemistry, Technische Universität Darmstadt

M

ETHODS

NMR S

PECTROSCOPY

• Solution NMR: structure determination of peptides and proteins, protein‐ligand interactions,

analysis of dynamics and conformation of peptides and proteins (standard techniques such as

NOESY, ROESY plus various spectral editing techniques)

• Solid state NMR: structure determination of membrane proteins in lipid environment, protein‐

ligand interactions, dynamics and conformation analysis of proteins and peptides, NMR

crystallography (standard solid state NMR techniques such as CP‐MAS, REDOR, HETCOR, DARR,

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F‐CODEX, DIPSHIFT)

• Software: assignment/ data analysis: CCPN analysis, TopSpin 3.2, structure calculation: YASARA

structure, XPLORE‐NIH, Talos

C

HEMISTRY AND PEPTIDE CHEMISTRY

:

• Chemistry: Schlenk techniques, organo metallic synthesis, protecting group chemistry for amino

acid derivates

• Peptide chemistry: Solution phase synthesis, solid phase synthesis, ligation strategies, manual

and automated syntheses

• Targets compounds: amino acid derivatives, short, medium‐sized and long peptides,

miniproteins, membrane proteins, peptidomimetics

• Peptide analytics: analytical and semipreparative HPLC (RP, IEC), TLC

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B

IOCHEMISTRY

/P

ROTEIN BIOCHEMISTRY

:

 Protein‐protein and protein‐ligand interactions: spectroscopic methods (e.g. NMR, UV/Vis);

 Enzymology: activity measurements, catalytic mechanism of enzymes, enzyme inhibitors, UV‐

Vis and fluorescent enzymatic assays (Stopped Flow, HPLC)

 Mass spectrometry of peptides and proteins: MALDI‐TOF/TOF‐MS

T

HEORETICAL CHEMISTRY

:

•

Protein dynamics/ motion analysis: Molecular dynamics simulation (NAMD, YASARA structure),

targeted molecular dynamics simulation, normal mode analysis

•

Protein‐ligand interactions: molecular docking (Autodock, Autodock VINA, Rosetta docking)