OWLS 2012 PROGRAM. OWLS Genoa, 4-6 July 2012 Program Page 1

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OWLS 2012

ORGANISING COMMITTEE

Alberto Diaspro, Istituto Italiano di Tecnologia and University of Genoa, Italy

Francesco Baldini, National Research Council, Florence, Italy

Paolo Bianchini, Istituto Italiano di Tecnologia, Genoa, Italy

Francesca Cella Zanacchi, Istituto Italiano di Tecnologia, Genova, Italy

Valeria Delle Cave,Istituto Italiano di Tecnologia, Genova, Italy

Zeno Lavagnino, Istituto Italiano di Tecnologia and University of Genoa, Italy

Mattia Pesce, Istituto Italiano di Tecnologia, Genova, Italy

Manuela Rigano, University of Genova, Italy

Manuela Salvatori, Istituto Italiano di Tecnologia, Genova, Italy

Federico Tixi, Istituto Italiano di Tecnologia, Genova, Italy

Cesare Usai, National Research Council, Genova, Italy

PROGRAM

Alberto Diaspro, Istituto Italiano di Tecnologia and University of Genoa, Italy

Silvio Aime, University of Turin, Italy

Francesco Baldini, National Research Council, Florence, Italy

Gert von Bally, University of Muenster, Germany

Paolo Bianchini, Istituto Italiano di Tecnologia, Genoa, Italy

Francesca Cagnoni, Istituto Italiano di Tecnologia, Genova, Italy

Halina Rubenstein-Dunlop, University of Queensland, Australia

Hans-Jochen Foth, Technical University of Kaiserslauter, Germany

Min Gu, Swinburne University, Australia

Partha Pratim Mondal, Indian Institute of Science, Bangalore, India

Stephen Morgan, University of Nottingham, UK

Colin JR Sheppard, National University of Singapore, Singapore

Cesare Usai, National Research Council, Genova, Italy

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Page 2 • Program • OWLS • Genoa, 4-6 July 2012

Exhibit Area

2nd Floor

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Nikon Instruments Spa

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IIT - Istituto Italiano di Tecnologia / LANIR.EU

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Physik Instrumente (PI) S.r.l.

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HAMAMATSU PHOTONICS ITALIA

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Till Photonics GmbH

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Fianium Limited

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3rd Floor

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OLYMPUS ITALIA SPA

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VUTARA Inc.

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TOPTICA Photonics AG

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Bitplane AG

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OWLS 2012

Tuesday, July 3rd - 7.00pm

Inaugural Ceremony

Sala del Maggior Consiglio, Palazzo Ducale

www.palazzoducale.genova.it

Alberto Diaspro

,

OWLS President

Roberto Cingolani

,

Scientific Director, Istituto Italiano di Tecnologia

“Lettura Magistrale”

Scott E. Fraser

,

Caltech, CA

Imaging the Molecular and Cellular Dynamics of Embryogenesis

Welcome Cocktail kindly offered by

Camera di Commercio di Genova

Leica Microsystems

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Wednesday, July 4th

9.00 - 9.30

Magazzini del Cotone - Congress Center

http://www.portoantico.it

Scirocco-Libeccio Room

OWLS 2012 Opening Lecture

Karsten Konig, Saarbrucken University, Germany

Clinical Multimodal Hybrid Multiphoton/CARS Tomography of In Vivo Human Skin

Chair: A. Diaspro Parallel Sessions

9.30 - 10.00

Scirocco - Libeccio Room Ponente Room

David Sampson

University of Western Australia, Australia

Optical elastography - high-resolution imaging of tissue mechanical properties.

Chair: G. Scarcelli

Gertrude Bunt

University of Goettingen, Germany

Visualization of multiple coupled signal-ing events in a ssignal-ingle cell by fluorescence lifetime pattern analysis.

Chair: R. Bizzarri

10.00 - 10.45 Optical application to biomedicine

Chair: G.Scarcelli

Andrea Curatolo

University of Western Australia, Australia

Portable 3D optical coherence elastogra-phy for applying microscopic imaging of tissue mechanical properties in the clinic.

George A. Stanciu

University Politehnica Bucharest, Romania

Electrical charge on the hydroxyapatite surface investigated by laser scanning microscopy.

S. Beke

Istituto Italiano di Tecnologia (IIT), Gen-ova, Italy

Excimer laser-based stereolithography for rapid fabrication of rigid biodegrad-able photopolymer scaffolds for tissue engineering.

Advanced imaging techniques I

Chair: R. Bizzarri

Barbara Storti

NEST, Pisa Italy

Multiplexing correlation spectroscopy and resonance energy transfer unveils dy-namical interactions between transient receptor potential vanilloid 1 (trpv1) and microtubules at nanoscale in living cells.

Dominika O. Trembecka-Lucas

Jagiellonian Universit. Poland

Interaction between HP1 dimer and PCNA in DNA replication and repair, as revealed by bimolecular fluorescence complementation assay (BiFC), and FRET/FLIM.

Simona Rodighiero

Fondazione Filarete, Milan, Italy

FRET study of 4.1R, ICln and actin inter-actions: a link between cell morphology and cell proliferation.

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10.45 - 11.15 Giuliano Scarcelli

Harvard Medical School, USA

Brillouin microscopy to study the bio-mechanical properties of cornea and lens.

Chair: D.Sampson

Ranieri Bizzarri

National Research Council, Italy

Novel probes and strategies for high-res-olution fluorescence imaging in living cells.

Chair: G.Bunt

11.15 - 11.45 Coffee break

11.45 - 12.15

Scirocco-Libeccio Room

Monika Ritsch-Marte

Medizinische Universität Innsbruck, Austria

Applications of spatial light modulators in biophotonics: from optical tweezers to advanced microscopy.

Chair: F.Difato Parallel Sessions

12.45 - 13.30

Scirocco - Libeccio Room Ponente Room

Plasmonics

Chair: E. Di Fabrizio

Emanuela Esposito

CNR-ICIB Napoli, Italy

Lab-on-Fiber Technology: Nanophoton-ics Within Optical Fibers.

Fang Liu

Tsinghua University, Beijing

Integrated Bio-Sensor based on Hybrid Coupler with Surface Plasmon Polariton and Dielectric Waveguide.

Ranjeet Kumar

Indian Institute of Technology New Delhi, India

Clustering of Au-NPs near plasmon ex-citation and enhanced scattering based in-vitro detection of Ehrlich ascites car-cinoma cells.

Advanced imaging techniques II

Chair: A. Periasamy

Antonella Battisti

NEST, Pisa, Italy

Intracellular pH measurements made simple by genetically-encoded fluo-rescent probes and phasor approach to FLIM.

Ottavia Golfetto

University of California, Irvine, California

Laurdan fluorescence lifetime as a bi-osensor for membrane fluidity.

Deo R. Singh

University of Wisconsin Milwaukee, USA

Quaternary structure of wild type and mutated rhodopsin at room and body temperature using spectral FRET. 13.30 - 15.30 Lunch, posters, exhibition, Job corner.

Company Presentations

14.00 - 15.00

Scirocco - Libeccio Room Ponente Room

Olga Levai

Leica Microsystems

Opening the gate to Super-Resolution.

Maria Marosvölgyi

Till Photonics

Combining advanced fluorescence imag-ing techniques on the same livimag-ing sample: spinning disk confocal, TIRF, FRAP(PA) and FRET.

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15.30 - 16.00

Scirocco - Libeccio Room

Anthony Watts Oxford University, UK

Realizing bio-imaging potential: Enhancing bacteriorhodopsin-mediated molecular scale, wavelength-selective photoswitching.

Chair: A. Periasamy Parallel Sessions

16.00 - 16.30

Scirocco - Libeccio Room Ponente Room

Ilaria Testa

Max Planck Institute Goettingen, Germany

RESOLFT Nanoscopy in Life Sciences: unraveling the mysteries with low light levels

Chair: G. Vicidomini

Arthur Chiou

National Yang-Ming University, Taiwan (R.O.C.)

Optical Tweezers Based BioMicrorheology

Chair: D. Cojoc

16.30 - 17.15 Single molecule and super-resolution

Chair: I.Testa

Paolo Annibale

EPFL, Lausanne, Switzerland

Towards accurate dual color PALM im-aging.

Francesca Cella Zanacchi

Istituto Italiano di Tecnologia, Genova, Italy

Super-resolution microscopy of large biological samples within a light sheet based architecture.

Davide Mazza

Fondazione S.Raffaele, Milano, Italy

Measurement of binding in living cells by single molecule tracking. A benchmark for protein/scaffold interactions.

Optical Tweezers

Chair: A. Chiou

Francesco Difato

Istituto Italiano di Tecnologia, Genova, Italy

Position and Force-clamp interferometric tracking of an optically trapped probe to study cytoskeleton dynamics of devel-oping mouse neuron.

Pasquale Bianco

Università degli Studi di Firenze, Italy

The energy landscape of DNA over-stretching transition determined with a Dual Laser Optical Tweezers under force clamp.

Dan Cojoc

Materials Technology Institute, Trieste, Italy

Local delivery of small numbers of attrac-tant and repulsive guidance molecules to neuronal cells by optical manipulation techniques.

17.15 - 17.45 Mike Heilemann

Wuerzburg University, Germany

Quantitative Single Molecule Biology with Photoswitchable Fluorophores.

Chair: I.Testa

Maria Luisa Calvo Padilla

Universidad Complutense de Madrid, Spain

Holography and its applications.

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17:45 - 18:15 Coffee break

18:15 - 18:45

Scirocco - Libeccio Room

Brad Amos Cambridge, UK

A laser-scanning microscope for optical sectioning of large-volume specimens such as mouse embryos using a Mesolens.

Chair: C.J.R. Sheppard Parallel Sessions

18.45 - 19.30

Scirocco - Libeccio Room Ponente Room

Optical tomography

Chair: C.J.R. Sheppard

Stephen P Morgan

University Park, Nottingham, UK

Pulsed ultrasound modulated optical to-mography of tissue

Maria Pogorelova

Moscow State University, Moscow, Russian Federation

Optical microtomography of early mouse embryo with laser scanning microscopy

Ludovico Silvestri

University of Florence, Italy

Whole-brain neuroanatomy on the cron-scale with confocal light sheet mi-croscopy

Optical application to biomedicine II

Chair: K. Braeckmans

Wei Zhang

Tsinghua University, Beijing, China

Trace level multi-gas sensing in mid-in-frared hollow-core Bragg fiber supporting multi-wavelength transmission

Tadelech

Laser-Induced Breakdown and X-Ray Fluorescence Spectroscopic Techniques in Detection of Elements in Tea Leaves

Tewfik Soulimane

University of Limerick, Ireland

Photosterilisable Textiles for Improved Hospital Hygiene

19.30-20.00 Martin J. Booth

Oxford University, UK

Adaptive optics for high resolution bio-logical microscopy

Chair: C.J.R. Sheppard

Dayong Jin

Macquarie University, Sydney, NSW 2109, Australia

Novel coding and decoding in suspension arrays for accelerated biomolecular dis-covery and personalized medicine.

Shan Shan Kou

EPFL, Lausanne, CH-1015 Switzerland

Three-dimensional (3D) Monte Carlo Simulation of Born’s Diffractions for Cel-lular Analysis

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Thursday, July 5th

8.30 - 9.00

Scirocco - Libeccio Room

RMS Lecture

Tony Wilson Oxford University, UK

Making Light Work in Microscopy.

Chair: F. Brakenhoff Parallel Sessions

9.00 - 9.30

Scirocco - Libeccio Room Ponente Room

Martin Oheim

Université Paris Descartes, France

Non-evanescent excitation light in ob-jective-type TIRF microscopy limits to quantitative imaging and super-resolu-tion microscopy.

Chair: J. Bewersdorf

Amitabha Chattopadhyay

Centre for Cellular and Molecular Biology Hyderabad, India

Organization and Dynamics of the Se-rotonin-1A Receptor in Live Cells using Fluorescence Microscopy.

Chair: S. Maiti

9.30 - 10.15 STED microscopy I

Chair: J. Bewersdorf

Giuseppe Vicidomini

Istituto Italiano di Tecnologia, Genoa, Italy

Towards Low-Intensity STED Microscopy by Time Gating.

Lorena Benedetti

University of Milan, Italy

Morphological features of 4.1R overex-pressing cells characterized with Correla-tive Light-Scanning Electron Microscopy and observed with gated-STED nanoscopy.

Tilman Rosales

National Institutes of Health, USA

Instrumentation for super-resolution us-ing new low power probes.

Advanced Imaging Techniques III

Chair: A. Chattopadhyay

Cosimo Arnesano

University of California Irvine, Irvine, Cal-ifornia

Digital Parallel Frequency-Domain Spec-troscopy for Tissue Imaging.

Stefano Coppola

Sapienza - University of Rome, Italy

Intracellular trafficking of multicompo-nent lipoplexes: a fluorescence micros-copy study.

Anna Letizia Allegra Mascaro

University of Florence, Italy

In vivo imaging of climbing fibers reactive plasticity after laser axotomy.

10.15 - 10.45 Joerg Bewersdorf

Yale University, USA

New Approaches in STED Microscopy: TIRF and Phase Retrievaly.

Chair: M. Oheim

Sudipta Maiti

Tata Institute of Fundamental Research, India

Protein misfolding versus aggregation: following the early events with an ultra-sensitive fluorescence tool.

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10:45 - 11:15 Coffee break

11.15 - 11.45

Scirocco - Libeccio Room

Ernst H.K. Stelzer

Goethe University Frankfurt , Germany

From the ultramikroskop to theta microscopy to tetrahedral microscopy to light sheet-based fluorescence microscopy.

Chair: F. Cella Zanacchi Parallel Sessions

11.45 - 12.30

Scirocco - Libeccio Room Ponente Room

Single Particle Tracking

Chair: Z. Lavagnino

Hendrik Deschout

Ghent University, Belgium

On-chip sheet illumination for sin-gle-particle tracking measurements in bio-fluids.

Katrien Forier

Ghent University, Belgium

Single particle tracking microscopy: To-wards nanomedicines for the treatment of chronic pulmonary infections in cystic fibrosis patients.

Christelle Rosazza

University of Konstanz, Germany

Single Particle Tracking of DNA in cells delivered by electroporation.

Label Free Techniques

Chair: P. O’Shea

Francesca Romana Bertani

National Research Council, Rome, Italy

Hyperspectroscopic confocal reflectance imaging of cultured cells.

Alessandro Candiani

University of Parma, Italy

Label-free DNA sensor based on optical fiber grating devices.

Dan Cojoc

National Research Council, Trieste, Italy

Secondary speckle sensing microscopy for fast detection of malaria.

12.30 - 13.00 Kevin Braeckmans

University of Ghent, Belgium

Sizing Nanomatter in Biological Fluids by Fluorescence Single Particle Tracking.

Chair: Z. Lavagnino

Paul O’ Shea

University of Nottingham, UK

Imaging specific proteins in cells; time-resolved dynamics and disposition without GFP.

13.00 - 15.00 Lunch, posters, exhibition, Job corner. Company Presentations

14.00-15.00

Scirocco-Libeccio Room

Luciano Lucas

Bitplane

Imaris: the 3D/4D image analysis soft-ware

Ponente Room

Alessandro Cometta

Zeiss

A super-resolution close-up view: from SIM to PALM

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15.00 - 15.30 Scirocco-Libeccio Room Colin Sheppard

National University of SIngapore, Singapore and Istituto Italiano di Tecnologia, Italy

Imaging in the phase contrast microscope.

Chair: P.Saggau

15.30 - 16.00 Gail McConnell

University of Strathclyde, UK

Promising new wavelengths for multiphoton laser scanning microscopy: thinking outside the Ti:Sapphire

Chair: P.Saggau Parallel Sessions

16.00 - 17.30

Scirocco - Libeccio Room Ponente Room

Non Linear/Multiphoton Microscopy

Chair: G. McConnell

Giulia Borile

University of Padova, Italy

Multiphoton fluorescence Ca2+ imaging in acute cardiac slices.

Albrecht Haase

University of Trento,Italy

In-vivo morpho-functional imaging of the honeybee brain .

B. Leshem

Paris Descartes University

Temporally focused illumination is inher-ently robust against scattering.

Leonardo Sacconi

National Research Council, Florence, Italy

Optical recording of intracellular action potential propagation in cardiac myo-cytes.

Godofredo Bautista

Tampere University of Technology, Fin-land

Wide field third-harmonic generation microscopy of turbid media.

Neeven A. Hosny

Imperial College London, Great Britain

Viscosity imaging of lipid-based micro-bubbles using fluorescence lifetime im-aging (FLIM) of molecular rotors.

Fluorescent Probes/Sensors

Chair: F. Brandi

Komal Bagga

Laser-based generation of luminescent bio-nanomarkers for cell imaging.

Ilaria De Santo

Istituto Italiano di Tecnologia, Naples, Italy

Understanding Drug Localization in Dox-orubicin Loaded TiO2 Nanotube Arrays through Fluorescence Correlation Spec-troscopy analysis.

Joanna L Richens

University of Nottingham, UK

Plasmonic-Based Biosensors in Disease Diagnosis.

Shu-Han Wu

National Yang-Ming University, Taipei, Taiwan

Dynamic Study of Cell Secretion using Microfluidic Traps and Gold Nanoslits Based Surface Plasmon Sensors.

Milena Stephan

Georg-August University of Goettingen, Germany

Combining Fluorescence Microscopy with Reflectometry: Development of a Biomembrane Sensor.

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17.30 - 18.00

Scirocco - Libeccio Room

Francesco S. Pavone

European Laboratory for Non Linear Spectroscopy, University of Florence

In Vivo Reactive Neural Plasticity Investigation by means of Correlative Light-Elec-tron Microscopy.

Chair: P. Bianchini

18.00 - 19.15 20.30

OWLS Assembly with Coffee break Social Dinner

Villa dello Zerbino

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Friday, July 6th

9.00 - 9.30

Scirocco - Libeccio Room

Enrico Gratton

University of California Irvine, US

Super-resolution by feedback imaging: mechanisms of translocation through the nuclear pore complex.

Chair: P. Bianchini

9.30 - 10.00 Daniel Farkas

Cedars-Sinai Medical Center, USA

Multimode Optical Molecular Imaging of Disease for Better Diagnosis and Treatment.

Chair: P. Bianchini Parallel Sessions

10.00 - 10.45

Scirocco - Libeccio Room

Ponente Room

Amyloid Studies

Chair: C. Canale

Valeria Musi

Ecole Polytechnique Fédérale de Lausanne, Switzerland

Bloch surface wave optical platform for sensing the aggregation kinetics of amy-loid beta peptides. Towards the early de-tection of Alzheimer’s disease.

Bidyut Sarkar

Tata Institute of Fundamental Research, Mumbai, India

Label free dopamine imaging with multi-photon ultraviolet microscopy.

Niels Zijlstra

University of Twente, Netherlands

Establishing the composition of al-pha-synuclein amyloid oligomers using single-molecule photobleaching.

Optics in the environmental science

Chair: F. Baldini

Malgorzata Biczysko

Istituto Italiano di Tecnologia, Pisa, Italy

Simulation of optical spectra line-shapes for biomolecules in natural environmen-tal conditions.

Sanjaya Thilakarathne

Saitama University, Japan

Ultra short term growth dynamics of Soybean and Radish under ozone stress using Statistical Interferometry Tech-nique - a comparison with conventional ozone assessment measures.

Dayong Jin

Macquarie University, Sydney, Australia

Tuning the Rare-earth Nanoparticles for Background-free Biosensing.

10.45 - 11.15 Coffee break

11.15 - 11.45

Scirocco - Libeccio Room

Nicolai Urban

Max Planck Institute Goettingen, Germany

Dynamic superresolution microscopy deep inside living brain tissue.

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Parallel Sessions

11.45 - 12.30

Scirocco - Libeccio Room

Ponente Room

Sted Microscopy II

Chair: B. Harke

Paolo Bianchini

Single wavelength 2PE-STED nanoscopy.

Brian R. Rankin

Mobius Photonics, CA, USA

A new laser for STED

Jay R. Knutson

National Institutes of Health, USA

A new class of low-power probes for mul-ticolor nanoscopy.

Novel Techniques

Chair: E. Gratton

Cristophe Silien

University of Limerick, England

Chemical Imaging with Super Resolution Infra-Red Absorption.

P. Nandakumar

BITS Pilani K. K. Birla Goa Campus, India

Laser scanning photothermal microscopy.

William M. Whelan

Atlantic Veterinary College, Canada

Frequency spectrum analysis in pho-toacoustic tissue characterization. 12.30 - 13.00 Peter Saggau

Baylor College, Texas, USA

Advanced Optical Imaging for Re-verse-Engineering the Brain

Chair: B. Harke

Ari T. Friberg

National Institute of Advanced Industrial Science and Technology (AIST), Japan

Classical Ghost Imaging of Phase Objects and through Phase Aberrations

Martijn Stopel

University of Twente, Netherlands

Room temperature excitation spectros-copy of single quantum dots

13.00 - 15.00 Lunch, posters, exhibition, Job corner.

15.00 - 15.30

Scirocco - Libeccio Room

Ponente Room

Maria Garcia Parajo

ICFO- Institut de Ciences Fotonique Barcelona, Spain

Cell surface nanoscopy using single molecule optical nanotools

Chair: M. Heilemann

15.30 - 16.00

EBSA Lecture

Andreas Zumbusch

Universitat Konstanz, Germany

Coherent anti-Stokes Raman Scattering microscopy of intracellular dynamics

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Parallel Sessions

16.00 - 17.30

Scirocco - Libeccio Room

Ponente Room

Nanoparticles & Smart Materials

Chair: M. Lorenzoni

Athanassia Athanassiou

Nanocomposite surfaces with photoin-duced wettability gradient for directional growth of cells.

Francesco Baldini

National Research Council, Floresnce, Italy

PMMA-nanoparticles for molecular bea-con delivery in cells for survivin mRNA detection.

Despina Fragouli

Istituto Italiano di Tecnologia, Lecce, Italy

Rendering cellulose sheets antibacterial using silver nanoparticles formed by a laser ablation technique.

Alemu Kebede

Addis Ababa University, Etiopia

Controlled Synthesis, Characterization and Application of Iron Oxide Nanopar-ticles for Oral Delivery of Insulin.

Enrico Rampazzo

Università di Bologna, Italy

Fluorescent Silica-Core/PEG-Shell Nano-particles with Tunable emission for Imag-ing and LabelImag-ing.

Laura D’Alfonso

Università degli Studi di Milano Bicocca, Milan, Italy

Intracellular diffusion of Gold Branched Nanoparticles.

Force Microscopy

Chair: C. Canale

Marco Capitanio

European Laboratory for Non-linear Spec-troscopy, Sesto Fiorentino, Italy

Ultrafast force-clamp spectroscopy of single biomolecules

Josè L.Toca-Herrera

University of Natural resources and Life Sciences Wien, Austria

Delivering mechanical and interfacial sample properties by combining optical and atomic force microcopies

Jenu Varghese Chacko

AFM STED: Targeted probing with high spatial precision

Raman Spectroscopy

Chair: R. Proietti

Riccardo Cicchi

European Laboratory for Non-linear Spec-troscopy, Sesto Fiorentino, Italy

A combined fluorescence-Raman spec-troscopic probe for the diagnosis of melanocytic lesions

Anna Chiara De Luca

Institute of Protein Biochemistry, Naples, Italy

Modulated Raman spectroscopy: a promising biophotonics tool for early cancer diagnosis

Nicholas Smith

Osaka University, Japan

Raman imaging and analysis: from quan-tification of cellular morphology to RNA structure and intramolecular interac-tions.

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17.30 - 18.00

Closing Ceremony

Scirocco-Libeccio Room

OWLS 2012 Closing Lecture

Satoshi Kawata

Osaka University, Japan

Plasmons, Raman, nano and 3D, all in together.

Chair: A. Diaspro

18.00 - 18.30

Poster prizes

Sponsored by OWLS, Université Laval Québec, Camera di Commercio

di Genova

Farewell with Focaccia and white wine

Sponsored by Camera di Commercio, Leica Microsystems, Bitplane

Friday, July 6 2012 8.30pm

City Lights by LAMBS

www.lambs.it

Piazza De Ferrari, Genova (De Ferrari Square)

Saturday, July 7 2012 Full Day

Cinque Terre Tour

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OWLS 2012

Poster Session

“An insight of NoBoDys PerFect” Exhibition

P1 - P6 Page

P1 Gaser N. Abdelrasoul1,2 ,_{Raffaella Magrassi}3_{, Marco Scotto d’Abbusco}1_{, Alberto}

Diaspro1, 2

1 Department of Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, 16163 Genova, Italy 2 Department of Physics University of Genova, via Balbi 5, 16126 Genova, Italy

3 National Research Council (CNR) - Institute of Marine Sciences (ISMAR), Genova, Italy

2PE Imaging of Cancer Cells Using PEGylated Gold Nanorods as Biocompatible Con-trast Agent.

120

P2 Giulio Caracciolo1_{, Daniela Pozzi}1_{, Anna Laura Capriotti}2_{, Chiara Cavaliere}2_,

Francesco Cardarelli3, Angelo Bifone3, Giuseppe Bardi3, Fabrizio Salomone3 and

Aldo Laganà2

1 Department of Molecular Medicine, ‘Sapienza’ University of Rome, Viale Regina Elena, 324, 00161, Rome, Italy

2 Department of Chemistry, ‘Sapienza’ University of Rome, P.le A. Moro 5, 00185, Rome, Italy, 3 Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro 12, 56127 Pisa, Italy

Protein corona controls the uptake delivery mechanisms of lipid gene vectors in can-cer cell

123

P3 Tatjana Dramićanin, Lea Lenhardt, Ivana Zeković, Nasta Tanić and Miroslav D. Dramićanin

Vinča Institute of Nuclear Sciences, University of Belgrade, P.O. Box 522, 11001 Belgrade, Serbia

Breast Cancer Detection by Support Vector Machine Classification of Synchronous Fluorescence Spectroscopy Data

128

P4 Ivana Zekovića, Lea Lehnardta*, Tatjana Dramićanina, Miroslav D. Dramićanina

a Vinča Institute of Nuclear Sciences, University of Belgrade, PO Box 522, 11001 Belgrade, Serbia

Application of Supervised Self-Organizing Maps in Skin Melanoma Diagnosis by Total Synchronous Fluorescence Spectroscopy

144

P5 Elisabetta M. Caspani, Philip H. Crossley, Carolina Redondo-Garcia, Salvador Martinez

Laboratory of Experimental Embryology, Instituto de Neurociencias UMH-CSIC, Alicante, Spain

Malignopodium: a new player in glioma progression

*

P6 Roberto Cighettia, Matteo Piazzaa, Valentina Calabresea, Gaetana Damorea and Francesco Peria

a Università degli Studi di Milano-Bicocca, Piazza della Scienza, 2 – 20126 – Milano, Italy

New fluorescent molecular tools to investigate LPS recognition

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P16 - P30 Page

P16 Hanna Beckera,2_{, Amy Gladfelter}3_{, Rudolf Oldenbourg}1

1 Marine Biological Laboratory, Woods Hole, Massachusetts, USA 2 Universität Konstanz, Konstanz, Germany

3 Dartmouth College, Hanover, New Hampshire, USA

Quantitative and high resolution imaging of fluorescence anisotropy is shedding light on septin architecture in the yeast bud neck

121

P17 Iván, Coto Hernández1,2_{, Giuseppe, Vicidomini}1_{, Alberto, Diaspro}1,2

1 Istituto Italiano di Tecnologia, Genova, Italia 2 Universita Degli Studi di Genova, Genova, Italia

Generation Of Super Continuum Source And Its Application In Nanoscopy

125

P18 William F. Falco, Joelson Fernandes, Evaristo A. Falcão, Anderson R.L. Caires

Grupo de Óptica Aplicada, Universidade Federal da Grande Dourados, Dourados, MS, Brazil

Effects on Photosynthetic Activity of Plants Induced by Silver Nanoparticles: Moni-toring by Chlorophyll Fluorescence

130

P19 Joelson Fernandes1_{, William F. Falco}1_{, Samuel L. Oliveira}2_{, Anderson R.L.}

Caires1

1 Grupo de Óptica Aplicada, Universidade Federal da Grande Dourados, Dourados, MS, Brazil 2 Grupo de Óptica e Fotônica, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil

Changes on Photosynthetic Activity of Herbicide-tolerant Soybean Plants Induced by Glyphosate

131

P20 Jenni Karttunen1_{, Sari Mäntynen}1_{, Heli Lehtivuori}1_{, Janne Ihalainen}1_{, Teemu}

Ihalainen1_{, Jaana K. H. Bamford}1_{and Hanna M. Oksanen}2

1 Department of Biological and Environmental Science and Nanoscience Center, PO Box 35, 40014 University of Jyväskylä, Jyväskylä, Finland

2 Institute of Biotechnology and Department of Biosciences, PO Box 56, 00014 University of Hel-sinki, HelHel-sinki, Finland

Protein Localization and Interactions in Bacteria Studied with Confocal Microscopy and Fluorescence Lifetime

138

P21 Volkan KILIÇ, Gözde AYDOĞAN KILIÇ, Ahmet ÖZATA

Anadolu University, Faculty of Science, Department of Biology, 26470, Eskişehir, TURKEY

Light microscopic pathology in Common carp ( Cyprinus carpio L.,1758) from Sa-karya River (TURKEY)

140

P22 Lea Lenhardt1_{*, Ivana Zeković}1_{, Tatjana Dramićanin}1_{, Živoslav Tešić}2_{, Dušanka}

Milojković-Opsenica2, Miroslav D. Dramićanin1

1 Vinča Institute of Nuclear Sciences, University of Belgrade, PO Box 522, 11001 Belgrade, Serbia 2 Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.

Assessment of Fluorescence and Infrared Spectroscopy Capabilities for the Determi-nation of Botanical Origin of Serbian Unifloral Honey

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P16 - P30 Page

P23 Daniela Pozzi1_{, Giulio Caracciolo}1_{, Francesco Cardarelli}2_{, Angelo Bifone}2_{, Heinz}

Amenitsch3

1 Department of Molecular Medicine, “Sapienza” University of Rome, Viale Regina Elena, 324, 00161, Rome, Italy

2 Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro 12, 56127 Pisa, Italy

3 Institute of Biophysics and Nanosystems Research, Austrian Academy of Sciences, Schmiedel-strasse 6, A-8042 Graz, Austria

Confocal microscopy makes visible cholesterol-mediated gene delivery mechanisms 152

P24 Liliia Rohoza, Tatyana Dyubko, Sergey Galchenko, Boris Sandomirsky

Institute for Problems of Cryobiology and Cryomedicine fo the NAS of Ukraine, Kharkiv

Spectrofluorimetric investigation of interactions of heart peptides with blood serum albumin

153

P25 Sebastian Tannert1_{, Peter Kapusta}1_{, Alexander Glatz}1_{, Christian Litwinski}1_{, Uwe}

Ortmann1_{, Rainer Erdmann}1_{, Katrin Hoffmann}2_{, Ute Resch-Genger}2

1 PicoQuant GmbH, Rudower Chaussee 29, 12489 Berlin, Germany, www.picoquant.com 2 Federal Institute for Materials Research and Testing (BAM), Richard-Willstätter-Str. 11, 12489 Berlin, Germany)

On the spectral sensitivity calibration of fluorescence spectrometers: Extension to the NIR, polarization and grating effects

154

P26 Anatoliy Tatarets1*_{, Yevgeniy Povrozin}1_{, Olena Obukhova}1_{, Iryna}

Fedyunya-yeva1, Ewald Terpetschnig2, Leonid Patsenker1,2

1 State Scientific Institution “Institute for Single Crystals”, NAS of Ukraine, 60 Lenin Ave., 61001 Kharkiv (Ukraine)

2 SETA BioMedicals, LLC, 2014 Silver Ct East, Urbana, 61801, IL, USA,

Novel Squaraine Probes for Proteins and Fluorescence Imaging Applications

155

P27 Francesco Urciuolo1_{, Giorgia Imparato}1_{, Costantino Casale}1_{, Fabio Formiggini}1_,

Paolo Netti1,2

1 Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Largo Bar-santi e Matteucci n, 53 80125 Napoli

2 Interdisciplinary Research Centre on Biomaterials (CRIB), University of Naples Federico II, P. Le Tecchio 80, 80125 Naples, Italy

Coupling Engineered Living Tissue with Multiphoton Tomography : towards in vitro understating of biological phenomena

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P31 - P45 Page

P31 William Dallari, Marco Allione, Elena Samoylova, Marco Scotto d’Abbusco, Alberto Diaspro

Nanophysics, Istituto Italiano di Tecnologia, via Morego, 30, 16163 Genova

Permanent and rewritable 3D optical data storage through multi-photon absorption 126

P32 Silvia Galiani1_{, Benjamin Harke}1_{, Giuseppe Vicidomini}1_{, Gabriele Lignani}2_{, Fabio}

Benfenati2, Hanako Tsushima2, Evelina Chieregatti2, Paolo Bianchini1, Alberto

Diaspro1

1Nanophysics, Istituto Italiano di Tecnologia, Genoa, Italy

2Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Genoa, Italy

Optimization of a STED nanoscope

133

P33 Manuel Gunkela, Nina Beila, Jürgen Benekea, Slawek Mazura, Jürgen Rey-manna, Bastian Schumachera and Holger Erflea

a Viroquant-CellNetworks RNAi Screening Facility, BioQuant, University of Heidelberg, Germany

Automated microscopy in RNAi screening experiments

135

P34 Timo Hakkarainen1_{, Tero Setälä}1_{, Ari T. Friberg}1,2,3

1 Department of Applied Physics, Aalto University, Box 13500, FI-00076 Aalto, Finland

2 Department of Physics and Mathematics, University of Eastern Finland, Box 111, FI-80101 Joensuu, Finland

3 Department of Microelectronics and Applied Physics, Royal Institute of Technology (KTH), Elec-trum 229, SE-164 40 Kista, Sweden

Near-Field Imaging of Interacting Nano-Particles

136

P35 Christian Jüngst*and Andreas Zumbusch

Department of Chemistry, University of Konstanz, 78457 Konstanz, Germany

Label-free imaging of Lipid Droplets in living cells

137 P36 Marcelle Koenig1_{, Steffen Ruettinger}1_{, Sebastian Tannert}1_{, Thomas Schoenau}1_,

Christian Litwinski1, Olaf Schulz2, Michael Wahl1, Marcus Sackrow1, Kristian

Lauritsen1_{, Felix Koberling}1_{, Robert Ros}2_{, and Rainer Erdmann}1

1 PicoQuant GmbH, Rudower Chaussee 29, 12489 Berlin, Germany, [email protected] 2 Arizona State University, Lab Robert Ros, Physics Department, Tempe, AZ 85287-1504, USA

Pushing the boundaries of single molecule detection

141

P37 Zeno Lavagninoa1_{, Francesca Cella Zanacchi}1_{, Alberto Diaspro}1,2

1 Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30,16163 Genova, Italia 2 LAMBS- Dept. of Physics – University of Genoa, Via Dodecaneso 33, 16153 Genova, Italia

Thick Sample imaging and scattering effects: a phantom sample based characteriza-tion of Single Plane Illuminacharacteriza-tion Microscopy performances

142

P38 Aimar Namma2_{, Andres Arend}1_{, Marina Aunapuua,}2

1 Department of Anatomy, University of Tartu, Ravila 19, 50411 Tartu, Estonia

2 Department of Veterinary Medicine, Estonian University of Life Sciences, Fr Kreutzwaldi 62, 51014 Tartu

Computer assisted image analysis in the detection of expression of bone morphoge-netic proteins in the neural tube of human embryos

146

P39 Marco Zanella1_{†, Luca Ceseracciu}2_{†, Roberta Ruffilli}1_{, Alberto Diaspro}2_,

Liber-ato Manna1

1 Nanochemistry, Istituto Italiano di Tecnologia, via Morego 30, 16163 Genova, Italy 2 Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, 16163 Genova, Italy

Polarized plasmonic absorption in micrometer long copper(I) selenide nanowires

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P46 - P60 Page

P46 Abebe Belay

Adama Science and Technology University, School of Natural Sciences, Department of Physics, P.O.Box, 1888, Adama, Ethiopia,

The Hetero-Association of Caffeine (CA) with 5-Caffeoyl quinic Acid (5-CQA) and Ethidium Bromide (EB) in Aqueous Solution

122

P47 Mariagrazia Di Luca1_{*, Francesco Cardarelli}2_{, Ranieri Bizzarri}1_,3

1 NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, Piazza San Silvestro 12 - 56127 Pisa

2 Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro 12, 56127 Pisa

3 Istituto di Biofisica - CNR, via Moruzzi 1, 56124 Pisa

Fluorescent recombinant virus like particles as nano-carriers of sensors

127

P48 Sabato Fusco, Valeria Panzetta, Paolo Antonio Netti

Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia Largo Bar-santi e Matteucci, 53 80125 Napoli

Cell Mechanical Characterization by Particle Tracking Microrheology in Optical Mi-croscopy

132

P49 Gallus Lorenzo1*_{, Ferrando Sara}1_{, Gambardella Chiara}1_{, Amaroli Andrea}1_,

Faimali Marco2, Veronica Piazza2, Bianchini Paolo3, Masini Maria Angela1

1 Dip. di Scienze della Terra, dell’Ambiente e della Vita (DiSTAV), Univ. di Genova 2 Ist. di Scienze Marine, ICMM, CNR, Area di Genova

3 Dipartimento di Nanofisica, Istituto Italiano di Tecnologia Genova

Aquaporins in cyprid of barnacle Balanus amphitrite (=Amphibalanus amphitrite) (Cirripedia, Crustacea)

134

P50 Gözde Aydoağan KILIÇ, Volkan KILIÇ, Ahmet ÖZATA

Anadolu University, Faculty of Science, Department of Biology, 26470, Eskişehir, TURKEY

Autometallographic localization of heavy metals and immunoperoxidase detection of metallothionein induction in Common carp (Cyprinus carpio L.1758) from Sakarya River (TURKEY)

139

P51 Keurison F. Magalhães1,3_{, Anderson R.L. Caires}1*_{, Margarete S. Silva}2_{, Samuel L.}

Oliveira3

1 Grupo de Óptica Aplicada, Universidade Federal da Grande Dourados, Dourados, MS, Brazil 2 Grupo de Estudo e Desenvolvimento de Materiais, Universidade Estadual de Mato Grosso do Sul, Dourados, MS,Brazil

3 Grupo de Óptica e Fotônica, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil

The Role of Fatty Acid Methyl Esters on Optical Properties of Biodiesel

145

P52 Olena Obukhova1*_{, Anatoliy Tatarets}1_{, Olga Kolosova}1_{, Iryna Fedyunyayeva}1_,

Leonid Patsenker1,2, Ewald Terpetschnig2

1 State Scientific Institution “Institute for Single Crystals”, NAS of Ukraine, 60 Lenin Ave., 61001 Kharkiv (Ukraine)

2 SETA BioMedicals, LLC, 2014 Silver Ct East, Urbana, 61801, IL, USA

Highly Photostable Biomedical Markers Based on Thiosquarylium Dyes

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P53 Silvio Panettieri1_{Giovanni Signore}2_{Ranieri Bizzarri}2

1 NEST, Istituto Nanoscienze - CNR, Piazza S. Silvestro 12, 56127 Pisa

2 Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro 12, 56127 Pisa

Synthesis, stereochemical characterization, and photophysical features of an innova-tive 3-styryl coumarin acting as polarity/viscosity sensor for bioanalytical use

148

P54 Leonid Patsenker1,2*_{, Ewald Terpetschnig}2_{, Anatoliy Tatarets}1_{, Olena}

Obuk-hova1, Olga Kolosova1, Larysa Markova1, Inna Yermolenko1

1 State Scientific Institution “Institute for Single Crystals”, NAS of Ukraine, 60 Lenin Ave., 61001 Kharkiv (Ukraine).

2 SETA BioMedicals, LLC, 2014 Silver Ct East, Urbana, 61801, IL, USA,

Next Generation Fluorescent Dyes for Biomedical Applications

149

P55 Alessia Petrelli, Claudio Canale, Alberto Diaspro and Silvia Dante

Nanophysics Department, Fondazione Istituto Italiano di Tecnologia, Genova (Italy)

Quantitative evaluation of cell adhesion on patterned surfaces

150 P56 Ferruccio Pisanello1,2_*, _{Luigi Martiradonna}1_{, Godefroy Leménager}3_{, Alberto}

Bramati3_{, Massimo De Vittorio}1,4

1 Center for Bio- Molecular Nanotechnologies @UNILE, Istituto Italiano di Tecnologia, 73010 Arne-sano (Lecce), Italy

2 Center for Neuroscience and Cognitive Systems @UNITN, Istituto Italiano di Tecnologia, 38068 Rovereto (TN), Italy

3 Laboratoire Kastler Brossel, Université Pierre et Marie Curie, CNRS UMR 8552, Ecole Normale Supérieure, 75005 Paris, France

4 National Nanotechnology Laboratory, CNR- ‐Nano, Università del Salento, Dipartimento Ingegn-eria dell’innovazione, 73100 Lecce, Italy

Anisotropic colloidal nanocrystals as ultrastable polarized quantum emitters

151

P57 Kelly-Ann Vere, Joanna Richens and Paul O’Shea

Cell Biophysics Group, Institute of Biophysics, Imaging and Optical Science (IBIOS), School of Biol-ogy, University of Nottingham, UK NG7 2RD

Electrostatic mapping of molecular interactions on the cell surface; identification of novel cell-surface receptor systems

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Conference Sponsors

M I C R O S Y S T E M S

Diamond and Gold

OWLS Member Assembly

July 5th, 2012 in Genoa, Italy (17:30-19:00)

Porto Antico Congress Center – Magazzini del Cotone – Scirocco-Libeccio Room

Agenda

Extra content: Lecture by Francesco Pavone (see program)

1. Discussion of the Agenda 2. President’s Report 3. Update of bylaws 4. Elections

❚Board

❙President, Vice-President, Secretary, Treasurer ❙OWLS Representative to ICO

❚Regional Council Representatives

❙Africa, North America, South America, Asia, Australia/New Zealand, Europe ❚Standing Committees

❙Bylaws and Regulatory Statutes;

❙Technology Transfer, Standardization, and Technology Assessment; ❙Finances;

❙Education and Ethics

5. Meeting schedule

❚OWLS 13 Location ❚Co-Sponsoring

6. Treasurer’s report and decision of formal approval

❚Financial Report 7. New Members 8. Publications 9. Dues 10. Committee Reports 11. Miscellaneous Prof. Alberto Diaspro