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Does the chaperone heat shock protein hsp70 play a role in the control of developmental processes?

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Fig.1. Northernblothybridizationof stageVI oocyteandembryostageRNA. Total RNA of 10 oocytesor embryosfromunfertilizedegg totai/budstagewasfractionatedonagarosegels,stainedwitherhidiumbromideto evaluateloading and RNA integrity,transferredto mem-branesandhybridizedwirh P2Pl Pw70/534DNA probe.(AISignalcorre-spondingto an RNA of about 3kb was detectedat every 5tage.'285'and'18S'indicatethe migrationpositionof {RNA(8) As a controlof RNArntegrity, '28S' (RNA was visualized by ethldiumbromidestaining
Fig. 4. Immunolocalizationof HSP70-relatedand meraphaseizedproteinson Pfeurodelesembryosectionsshowingcleavagestages.(A) 2-cell embryosshowingthe cytoplasmicana perinuclearlocationof HSP70.(B) The S8melocarionis observedin anima!blastomeres.(C) Detailof a
Fig. 5. Immunolocalizationplasmof HSP70-relatedproteinat gastrulastages.(A,B)Earlygastrulastage.Immunofluorescencemainly concerns cyto-of all blastomeresand perinuclearregionsof some.Detailof blasropore(arrow)in B showsstrongfluorescenceof bottlecells.(C,D
Fig. 6. Immunofluorescenceof an exogastrulasection: nuclei of InvO-luted cells are labeled (small arrows), whereas rhose of cells which couldnot invaginare are not

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