Biochemistry 1 Course Specifications. First year of M.B.B.Ch. Program

Faculty of Medicine Al-Azhar University Assuit Quality Assurance Unit Faculty of Medicine

Biochemistry 1

Course Specifications

First year of M.B.B.Ch. Program

A- Professional information:

Title: Biochemistry1

Programme(s) on which the course is given: M.B.B.Ch. program Major or minor element of programs: Major

Department offering the program: Department of Biochemistry

Department offering the course: Department of Medical Biochemistry

Academic year / Level: First year

Date of specification approval: 21/ 10/ 2014

Teaching hours a. Lecture:

b.Tutorial/practical: Totalteaching hours:

120 hours 48 hours

168 hours Allocated marks: 150 marks

Course duration: 24 weeks of teaching with a final end of year exam.

Course director: Prof. Dr. Elsaeid Mostafa Elsaeid Elbawab Head of Medical Biochemistry Department Teaching staff: 2 professors, 2 assistant professors,5 lecturers

and 11 assistant lecturers and demonstrators.

I- Course Aims:

¶ To enable the student to be oriented with essential topics including:

¶ Micro- and macromolecules of carbohydrates, lipids, proteins, nucleotides and nucleic acids

¶ To enable the student to describe basic principles of molecular biology and protein synthesis

¶ To understand the basic biochemistry of vitamins, and enzymes

¶ To enable the student be oriented with the basic biochemistry of the hemoglobin and free radicals

II- Intended Learning Outcomes:

II- A) Knowledge and Understanding:

By the end of the course, students should be able to:

1.Mention definition, importance, chemical structure and classification of carbohydrates, lipids, and proteins

2. Illustrate the mode of action and kinetics of enzymes and their role in the diagnosis of diseases

3. Illustrate structure and role of vitamins as coenzymes and point out diseases produced by vitamins deficiency

4. Describe the biological transport and cell membrane and their biochemical, clinical and laboratory importance

5. Mention definition, importance and chemical structure of steroids 6. Describe nucleotides, nucleic acids and gene concept

7. Mention DNA replication, transcription and post transcriptional modification of RNA.

8. Define genetic code and protein synthesis

9. Describe regulation ofgene expression and mutation

10. Describe mechanism of DNA repair, DNA damage and diseases related to defective repair

11- Describe genetic engineering and recombinant DNA techniques, animal and human cloning

12- Describe principles of gene therapy

13- Describe the structure of proteins of the extra-cellular matrix

14- Describe the immune system, structure and types of immunoglobulin

15- Mention definition, types, sources and effect of free radicals and antioxidants

II- B) Professional Skills:

II- B1. Practical skills:

By the end of the course, students should be able to:

1.Identify laboratory reagents and instruments used in biochemistry laboratory.

2- Identify the physical and chemical properties of carbohydrates

3- Perform chemical tests to study the properties of lipids and fatty acid 4- Identify the physical and chemical properties of amino acids and proteins and estimation of total plasma proteins

5- Identify unknown solutions

6- Identify tests for recombinant DNA technology. 7- Identify methods for animal and human cloning 8- Identify techniques utilized in analysis of DNA, RNA

9- Perform tests important for determination of hemoglobin in blood sample.

II- B2. Intellectual skills:

By the end of the course, students should be able to:

1. Interpret the general scheme to reach the correct identification of unknown carbohydrate, lipid and protein solution

2. Correlate causes, mechanisms and effect of diseases based on knowledge of carbohydrate, protein or lipid biochemistry or molecular biology

3. Interpret different tests for genetic engineering and recombinant DNA technology

4- Comment on techniques utilized in analysis of DNA and RNA. II- C) Communication & Transferable skills:

By the end of the course, students should be able to:

1- Write reports and essay on the different scientific items in the field of biochemistry.

2- Report the biochemical results in printable sheets 3- Work in groups and team in laps

4- Use computer and internet to extract information and knowledge III. COURSE CONTENTS:

Total teaching hours: 168 hr

Subjects Lectures Tutorial&

Practical

Total teaching Hours

Carbohydrates chemistry 15 16 31

Lipids chemistry 10 8 18

Proteins and neucleoproteins chemistry

15 16 31

Enzymes 20 -

-

20

Molecular biology 20 - 20

Vitamins 20 -

-

20 Free radicals and antioxidant 5 -

-

5 Myoglobin andhemoglobin 5 -

-

5

Immunoglobulins 5 -

-

5

Xenobiotics 2 - 2

Principles of physical chemistry

3 - 3

General scheme 8

III-A) TOPICS:

1.Carbohydrates chemistry: monosaccharides,(physical propertieschemical properties) Disaccharides (physical propertieschemical properties). Polysaccharides(physical

propertieschemical properties)

2.Lipids chemistry: definition and classification,fatty acids (physical and chemical

properties),triacylglycerols, phospholipids,glycolipids,steroids,

3. Proteins and neucleoproteins chemistry:amino acids (classification, physical and chemical properties), proteins (structure, classification, physical and chemical

properties, four orders of protein structure), purines and pyrimidines,nucleosides

4. Enzymes:general properties, classification, purification, kinetic, inhibitors,

regulation of enzymes activities,

5. Molecular biology: DNA structure and function, RNA types and function, DNA organization, replication and repair, RNA synthesis, processing and modification RNAs processing, protein synthesis,mutations, regulation of gene expression

recombinant DNA technology

6. Vitamins: Types, structure, functions, deficiency manifestations and recommended daily allowance.

7.Free radicals and antioxidant: definition, sources, hazards

,

8

.

:

9.Immunoglobulins: structure, calcifications

,

,

.

11. Principles of physical chemistry:isomerism, expression of concentration, diffusion

osmotic pressure, viscosity, surface tension, adsorption and elution, solutions, the

,

III-B) PRACTICAL CLASSES

1. Carbohydrates chemistry:Molisch's test-Fehling & Barfoed's tests-Selivanoff'test- osazone test-iodine test

2.Lipid chemistry:acroline test-salting out

3.Protein chemistry:Biuret'test –Xanthoproteic reaction-Milon's reaction-Rosenheim's reaction- sulfur test- heat coagulation test-salting out test-experiments on urea

(sodium hypobromite test)-expirements on uric acid ( Murexide test) 4. General scheme

IV. TEACHING AND LERNING M ETHODS: IV-A) M ETHODS USED:

1. Lectures (general and small group lectures).

2. Practical classes (small group teaching, practice of laboratory skills, AV aids) 3.MCQs (formative assessment)

4.Assignment and reports

IV-B) M ETHODS FOR DISABLED STUDENTS: No special arrangements are available.

V-C) TEACHING PLAN:

Lectures:

5 lectures/week; one hour each between 8 a.m. and 2 p.m. according to the current time table in general lecture halls.

Practical classes and tutorials:

The students are divided into 4 groups. Each group has a 2-hour practical class once per week. Students of each group are divided into 2 subgroups. Both

subgroups rotate between tutorial class (the related subjects of the theoretical lectures with AV aids) and practical class.

Time plan:

Item: Time schedule Teaching hours Total hours

Lectures 5lectures/week; one hour each between 8,00 am and 2 p.m

5x24 weeks 120

Practical 2 hours every other week

2x12 24 Tutorial 2 hours every

other week

2x 12 24

Total 168

V- TEACHING AND LEARNING F ACILITIES: Facilities used for teaching this course include: - Lecture halls: provided by the Faculty.

- Small group classes: in the Department.

- Information technology / AV aids: available in computer- assisted classes in the Department.

department.

VI. STUDENT ASSESSMENT: VI-A. ATTENDANCE CRITERIA:

The minimum acceptable practical (and tutorial) attendance is 75%; students who fail to attend that percentage of activities will lose 5 marks out of the practical marks. Students need to attend at least 60% in order to attend for the final practical examination.

VI-B) ASSESSM ENT TOOLS:

Tool Purpose

Written examination Assessment of knowledge and understanding Oral examination Assessment of knowledge and understanding Practical examination Assessment of practical and intellectual and

general skills (check list)

VI-C) TIME SCHEDULE:

•Formative examinations: one held in January and the other in April for all students. Those who don't attend the examination(s)for acceptable reason(s); their marks will be raised as a proportion from the final written examination score.

•Final examination: at the end of the academic year (May) for all students. The exam is re-held in September for those who fail to pass the final exam or postpone it

VI-D) GRADING SYSTEM:

Examination Marks allocated

Formative examinations: January

April

20 10 Final examination:

Written Oral Practical

75 20 25

Total ₁₅₀

•The minimum passing score is 90 marks provided at least 36 marks are obtained in the final written examination.

FAIR 60-<65%.

FORMATIVE ONLY ASSESSMENTS

Student knows his marks after the formative exam. VI-E) EXAMINATION DESCRIPTION:

Examination Type Description Marks Formative Exam:

Jan

April

Written

A one--hour short essay questions.

20

Written Thirty-minute MCQ

10

Final Written A 3-hour written paper

composed of short essay type questions (45 marks) and MCQ (30 marks)

75 Practical How to deal with laboratory

instruments. Detection of carbohydrates, lipids, protein, urea and uric acid

25

Oral One oral examination station 20

Total 150

VII- LEARNING AND REFERENCE M ATERIALS: VII-A) BASIC M ATERIALS:

•Department Book: available for students to purchase from the faculty or department. •Overhead projections and computer presentations used during teaching in lectures and tutorial classes.

•Notes on the laboratory practical work.

VII-B)List of references

1- course notes:

Department course notes (lectures and practical) 2- Essential books:

Department course notes. 3- Recommended books: ¶ Harper's Biochemistry.

¶ Lippincott's Illustrated Biochemistry.