Microbiological
Analysis
M ic ro b io lo g ic a l AnalysisGridded Membrane Filters from Cellulose Nitrate (Cellulose Ester)
acc. to ISO Norms, Sterile and Individually Packed, for Colony Counts
These membrane filters are in accordance with the following norms: ISO 7704, ISO 7899-2, ISO 8199, ISO 9308-1 and EN 12780.
Sterile, individually packed filters have long become standard for routine microbiological quality control because of the user benefits they offer.
They are pre-sterilized and ready-to-use and save preparatory time. As they are indi-vidually packed, they avoid the possibility of contamination of remaining filters in opened packs and conform with GLP, having filter identification and lot number printed on each individual envelope.
The increasing demand on these filters required the construction of a new packaging machine with ultra-modern stamping. Each membrane is checked to ensure it is not damaged in any way, is positioned correctly with no slippage under the edge seal, has perfect grid printing and is free of particles. Each envelope is checked for readable lettering. Quality control par excellence!
The membrane filters
All membranes are made of cellulose nitrate, a material which assures effective retention with high flow rates and optimum colony growth. The printed grid with a size of 3.1+3.1 mm makes the counting easier, especially for higher bacteria counts and for microcolonies, but does not influence the growth. The various filter colors allow the best contrast to the colonies (see membrane filter types, chosen for the nutrient pad sets, page 122).
Hydrophobic edge membranes are used mainly in the sterility testing of solutions containing antibiotics.
Specifications for gridded filter types, as for filter type 113 (page 120).
SMART filter dispenser manually operated for membrane tapes.
An automatic dispenser will be available starting 2ndhalf of 2004. Membrane Filters M ic ro b io lo g ic a l Analysis
Order numbers for sterile, individually packed membrane filters Type 114, white with black grid, for bacteriological research 47 mm diameter In packs of 100 11403-047ACN 1.20 µm 11404-047ACN 0.80 µm 11405-047ACN 0.65 µm 11406-047ACN 0.45 µm 11407-047ACN 0.20 µm In packs of 1000 11403-047ACR 1.20 µm 11404-047ACR 0.80 µm 11406-047ACR 0.45 µm 50 mm diameter In packs of 100 11403-050ACN 1.20 µm 11404-050ACN 0.80 µm 11405-050ACN 0.65 µm 11406-050ACN 0.45 µm In packs of 1000 11406-050ACR 0.45 µm 11403-050ACR 1.20 µm
Type 130, grey (black after wetting) with white grid, for the detection of yeasts and moulds
47 mm diameter In packs of 100 13004-047ACN 0.80 µm 13005-047ACN 0.65 µm 13006-047ACN 0.45 µm In packs of 1000 13004-047ACR 0.80 µm 13006-047ACR 0.45 µm 50 mm diameter In packs of 100 13004-050ACN 0.80 µm 13005-050ACN 0.65 µm 13006-050ACN 0.45 µm In packs of 1000 13005-050ACR 0.65 µm 13006-050ACR 0.45 µm
Type 131, white with black grid and pink-colored hydrophobic edge, for microbiological examination of products that contain inhibitors With 3 mm edge
47 mm diameter, in packs of 100 13106-047ACN 0.45 µm
13107-047ACN 0.20 µm
50 mm diameter, in packs of 100 13106-050ACN 0.45 µm
13107-050ACN 0.20 µm With 6 mm edge Membrane Filters M ic ro b io lo g ic a l Analysis
Type 138, green with dark-green grid, for bacteriological research 47 mm diameter In packs of 100 13806-047ACN 0.45 µm In packs of 1000 13806-047ACR 0.45 µm 50 mm diameter In packs of 100 13806-050ACN 0.45 µm In packs of 1000 13806-050ACR 0.45 µm
Type 139, white with green grid, for the detection of E.coli, coliform bacteria and other types of bacteria 47 mm diameter In packs of 100 13903-047ACN 1.20 µm 13906-047ACN 0.45 µm In packs of 1000 13905-047ACN 0.65 µm 13906-047ACR 0.45 µm 50 mm diameter In packs of 100 13906-050ACN 0.45 µm In packs of 1000 13906-050ACR 0.45 µm
Type 113, white, no grid, for prefilter holder 16807, page 137
47 mm diameter, in packs of 100 11301-047ACN 8 µm
50 mm diameter, in packs of 100 11301-050ACN 8 µm
Dispenserfilter … SCM
47 mm diameter*, contains 3 boxes of 100 membranes
white|black 11406--47----SCM 0.45 µm
grey|white 13006--47----SCM 0.45 µm
green|dark green 13806--47----SCM 0.45 µm
* 50 mm are available on request
Membrane tapes packed without protective papers. They will be available starting 2ndhalf of 2004.
Order numbers for gridded membrane filters, non-sterile, not individually packed, for particle testing, microscopy and microbiology
Type 114, white with black grid
25 mm diameter, in packs of 100 11403-025N 1.20 µm 11404-025N 0.80 µm 11406-025N 0.45 µm 37 mm diameter, in packs of 100 11404-037N 0.80 µm 47 mm diameter, in packs of 100 11403-047N 1.20 µm 11404-047N 0.80 µm 11405-047N 0.65 µm Membrane Filters M ic ro b io lo g ic a l Analysis
Type 130, grey (black after wetting) with white grid 25 mm grid, in packs of 100 13006-025N 0.45 µm 47 mm diameter, in packs of 100 13004-047N 0.80 µm 13005-047N 0.65 µm 13006-047N 0.45 µm 50 mm diameter, in packs of 100 13004-050N 0.80 µm 13005-050N 0.65 µm 13006-050N 0.45 µm
Type 131, white with black grid and 3 mm pink-colored, hydrophobic edge
25 mm diameter, in packs of 100 13106-025N 0.45 µm 13107-025N 0.20 µm 47 mm diameter, in packs of 100 13101-047N 8.00 µm 13106-047N 0.45 µm 13107-047N 0.20 µm 50 mm diameter, in packs of 100 13101-050N 8.00 µm 13106-050N 0.45 µm 13107-050N 0.20 µm
Type 131, with 6 mm pink-colored, hydrophobic edge
47 mm diameter, in packs of 100 13106-047-HCN 0.45 µm
13107-047-HCN 0.20 µm
Type 138, green with dark-green grid 47 mm diameter In packs of 100 13806-047N 0.45 µm In packs of 1000 13806-047R 0.45 µm 50 mm diameter In packs of 100 13806-050N 0.45 µm In packs of 1000 13806-050R 0.45 µm
Type 139, white with green grid 47 mm diameter In packs of 100 13906-047N 0.45 µm In packs of 1000 13906-047R 0.45 µm 50 mm diameter In packs of 100 13906-050N 0.45 µm In packs of 1000 13906-050R 0.45 µm Membrane Filters M ic ro b io lo g ic a l Analysis
Dehydrated Media Pads in Petri Dishes, with Matching Membrane
Filters for Economical, Time-saving Microbiological Quality Control
Sartorius nutrient pad sets have been used successfully in combination with the membrane filter method for more than 20 years now. They ease and simplify a lot of microbiological procedures, due to their practical handling.
One nutrient pad set consists of a 50 mm pad, evenly impregnated with nutrient media, packed dry and sterile in a petri dish, plus a suitable membrane filter, tested for colony growth, sterile and individually packed. Directly after wetting with sterile, demineralised water, the nutrient pad set is ready to use.
Benefits for the user Economy.
No time-consuming and labour-intensive preparation of the nutrient media (sterilization, cleaning, etc.).
Easy handling.
Nutrient pad sets can also be used in laboratories without comprehensive microbiological equipment. Consistent quality.
During the production, each nutrient pad set batch is compared with the corresponding agar medium, in order to guarantee consistent quality and reproducible results. Trouble-free storage.
nutrient pad sets can be stored at room temperature in a warehouse, between 18 and 24 months depending on the type.
Order numbers for nutrient pad sets in petri dishes
Nutrient pad set Determinaton | Order
type Detection*: number**:
Azide Enterococci (1) 14051-047N
Bismut sulfite Salmonellae (1) 14057-047N
Caso Colony count (1) 14063-047N
Cetrimide Pseudomonas aeruginosa (2) 14075-047N
Chapman Staphylococci (2) 14074-047N
Chromocult E. coli and coliforms (7) 14087-047N
ECD E. coli (2) 14082-047N
Endo E. coli and coliforms (2) 14053-047N
Glucose-Tryptone Mesophilic bacteria and 14066-047N
thermophilic spore formers (2)
Lysine Wild yeasts (3) 14061-047N
Malt extract Yeasts and moulds (6) 14086-047N
McConkey Enterobacteria (2) 14097-047N
M-FC E. coli and coliforms (2) 14068-047N
Orange serum Acid-tolerant microbes (1) 14062-047N
Orange serum (pH 3.2) Acid-tolerant microbes (1) 14096-047N
R2A Colony count (1) 14084-047N
Sabouraud Yeasts and moulds (3) 14069-047N
Schaufus-Pottinger Yeasts and moulds (4) 14070-047N
Schaufus-Pottinger Yeasts and moulds (5) 14072-047N
Media M ic ro b io lo g ic a l Analysis
Order numbers for nutrient pad sets in petri dishes
Nutrient pad set Determinaton | Order
type Detection* number**
Teepol E. coli und coliforms (2) 14067-047N
Tergitol TTC E. coli und coliforms (2) 14056-047N
TGE Colony count (1) 14076-047N
Tomato juice Lactic acid bacteria, special
Leuconostoc oenos (1) 14079-047N
VLB-S7-S Beer spoiling bacteria (2) 14059-047N
Weman Leuconostoc mesenteroides (1) 14065-047N
Wallerstein Lactic acid bacteria and
other beverage spoilers 14089-047N
Wort Yeasts and moulds (3) 14058-047N
Wort (pH 4,4) Yeasts and moulds (3) 14078-047N
Yeast extract Colony count (1) 14090-047N
Special brochure available on request. Order no. SM-4017-e
Media M ic ro b io lo g ic a l Analysis
* A pack contains 100 sterile nutrient pad sets, each of them individually packed in a petri dish, and 100 sterile, individually packed membrane filters. The membrane filters are selected for optimum growth together with the corresponding nutrient media. The supplied membrane filter type is listed within brackets:
(1) = green with dark green grid, 0.45 µm pore size
(2) = white with green grid, 0.45 µm pore size (3) = grey (black after wetting) with white
grid, 0.65 µm pore size
(4) = white with green grid, 0.65 µm pore size (5) = white with green grid, 1.2 µm pore size (6) = grey (black after wetting) with white
grid, 0.8 µm pore size
