(1)http://genomebiology.com/2001/2/3/reviews/0002.1. Review. Jason R Swedlow and Angus I Lamond Address: Division of Gene Regulation and Expression, Wellcome Trust Biocentre, University of Dundee, Dow Street, Dundee DD1 5EH, UK.. comment. Nuclear dynamics: where genes are and how they got there. Correspondence: Jason R Swedlow. E-mail: j.swedlow@dundee.ac.uk. reviews. Published: 9 Month 2001 Genome Biology 2001, 2(3):reviews0002.1–0002.7 The electronic version of this article is the complete one and can be found online at http://genomebiology.com/2001/2/3/reviews/0002 © BioMed Central Ltd (Print ISSN 1465-6906; Online ISSN 1465-6914). DNA is highly organized spatially, both within domains of chromatin along each chromosome and within the nucleus as a whole. Recent studies suggest that chromatin localization can affect transcriptional and replicational activity. The similarity between the movements of chromatin nuclear bodies suggests a common mechanism that regulates nuclear dynamics.. There is now a wealth of information about the molecular interactions involved in regulating gene expression and DNA replication, and it is clear that large structural changes occur in chromatin as a consequence of changes in transcriptional or replicational activity [12]. It is now commonly accepted that. information. Chromatin positioning. interactions. The nucleus is a dynamic organelle. Its partitioning into different compartments, each formed without membrane boundaries, suggests that the nucleus may use a different organizing principle than that of much of the cytoplasm. It also implies that there is a transport system to deliver factors to their appropriate targets and to move macromolecules between compartments, in addition to the tremendous flux of macromolecules entering and leaving the nucleus through the nuclear pores. So, how do things localize and move in the nucleus? In this review, we discuss the positioning and movement of nuclear components and how these processes may impinge on critical nuclear functions.. refereed research. It is likely that nuclear architecture also influences chromatin structure, just as cytoplasmic organelles are affected by their localization. Efforts to understand the architecture of the nucleus have exposed a remarkable level of organization of chromatin. Individual chromosomes are seen to occupy separate, non-overlapping territories in the interphase nucleus [6-9]. Moreover, a number of nuclear bodies. are now known, usually identified by the presence of specific molecular markers and formed in the nucleoplasm without the use of membrane barriers [10,11]. These bodies may be sites where specific nuclear activities take place; for example, the production of ribosomal subunits takes place in the nucleolus.. deposited research. We have grown used to the linear representation of the genome as a cluster of coloured lines representing different chromosomes or sections thereof. Unfortunately, for those of us studying its properties, the structure of the genome in vivo is far more complicated. Each chromosome is a complex of protein and nucleic acid, assembled into higherorder chromatin structures and packed into the cell nucleus. It is this chromatin complex that is the substrate for transcription, replication, damage repair and recombination. But chromatin can exist in at least two forms, termed ‘open’ and ‘closed’, that differ in their accessibility to nuclear factors including transcriptional activators and the replication machinery. It now appears that processes governing the opening of chromatin may play key regulatory roles in the nucleus. Recent studies have shown that loci do indeed expand upon transcriptional activation [1,2]. Moreover, protein machines and post-translational modifications have been identified that are involved in mediating structural changes in chromatin [3-5].. reports. Abstract.

(2) 2 Genome Biology. Vol 2 No 3. Swedlow and Lamond. chromatin loci that are separated by many kilobases can nevertheless make contact and functionally interact. Changes in higher-order chromatin structure are important for function, although we don’t yet understand the mechanisms involved. A good example of the relationship between function and nuclear location is provided by the inactivated human X chromosome, or Barr body. In human females, the inactive X chromosome is maintained in a condensed, heterochromatic state and predominantly positioned at the periphery of the nucleus, juxtaposed to the nuclear envelope. Transcriptionally inactive heterochromatin is also often observed clustered along the nuclear envelope. Does the location of inactive heterochromatin reflect a regulated localization of chromatin to a specific subnuclear domain? It is possible that parts of chromosomes (or as in the case of the Barr body, whole chromosomes) that are repressed are localized to the periphery of the nucleus because they are simply excluded from the more interior portion. Recent evidence suggests, however, that the localization of chromatin loci is a regulated process with profound consequences for their activity. The key to these observations has been the combination of techniques for fluorescence in situ hybridization (FISH) that preserve the structure of the nucleus and high-resolution, multi-colour fluorescence microscopy. The gene density in human chromosomes is known to vary widely. Bickmore and colleagues [13] have explored the location of gene-rich and gene-poor chromosomes in human cells. Using FISH techniques that ‘paint’ whole chromosomes, they have visualized the relative position of chromosomes in the nucleus and correlated these with transcriptional activity. A statistical analysis of the positions of the relatively gene-poor human chromosome 18 and the gene-rich chromosome 19 showed that chromosome 18 was preferentially located near the nuclear periphery. After inhibiting transcription, the preferential localization of chromosome 18 to the nuclear periphery was no longer observed. These data suggest that chromosome location can be dependent upon active transcription. Most recently, the same workers have examined the disposition of all human chromosomes in the interphase nucleus [14], and the results reinforce the inverse correlation between proximity to the nuclear periphery and gene density. It is interesting to compare these results with previous observations showing that late-replicating chromatin is preferentially located at the nuclear periphery (Figure 1) and that high levels of transcription and of acetylated histone H4 are found in the nuclear interior [15,16]. These domains are stably maintained through a number of cell cycles, showing that they are a fundamental aspect of nuclear organization [9,16]. Collectively, these data suggest that in the crowded milieu of the nucleus, chromosome position may be finely tuned and dependent on transcription, replication, and possibly other nuclear activities.. Figure 1 Timing of replication correlates with chromatin location. HeLa cells were briefly pulsed with Cy3-dUTP to mark actively replicating regions of chromatin, then fixed and imaged on a fluorescence microscope. The cell on the left is late in S-phase and preferentially replicating heterochromatic regions, with those in the nuclear periphery marked by arrowheads. The cell on the right is at an earlier stage of S phase, when predominantly internal chromatin is replicated. See [15] and references therein for further details. Scale bar = 5 mm. If domains of heterochromatin are specifically localized to defined regions within the nucleus, this implies that both cisand trans-acting factors must mediate these events. It has been suggested that the nuclear periphery may be repressive by nature and may contain some factors that promote heterochromatin formation. Indeed, in yeast, localization of telomeres to the nuclear periphery requires the presence of the Sir proteins, factors that mediate transcriptional silencing at the telomere [8,17]. The requirement for specific factors that mediate heterochromatin formation and localization suggests that localization is unlikely to be an entirely passive event.. Position-effect variegation The phenomenon of position-effect variegation (PEV) provides further evidence that the localization of a chromatin locus has strong consequences for its activity, and that proximity to heterochromatin directly represses transcription. PEV was first observed in Drosophila, where chromosome rearrangements caused repression of a number of genes by moving them close to a large block of centric heterochromatin [18]. A classic example involves an inversion of the X chromosome that moves the white gene to a location near a large block of centric heterochromatin. Expression from the relocated white gene was usually repressed, but the level of repression differed between groups of clonally related.

(3) http://genomebiology.com/2001/2/3/reviews/0002.3. deposited research. Chromatin dynamics. interactions information. Direct methods have recently been used in living cells to analyse chromatin movements. The technique of marking a chromosome with a tandemly repeated array of lacO (transcription-factor-binding) sites and expression of a fusion protein made up of green fluorescent protein (GFP) and lacI (the cognate transcription factor) provides a way of detecting a specific chromatin locus in a live cell [35]. Once integrated into the Saccharomyces cerevisiae genome, the position of the marked locus can be tracked over time [36,37]. A plot of the distance a locus travels as a function of time provides a measure of the type of motion, whether it is passive or active, and its characteristics. Results of this type of study showed that yeast chromatin diffuses passively with a diffusion constant D = 5 x 10-12 cm2/sec within a confined nuclear subvolume with a radius R = 0.3 mm [36]. Treatment of the cells with sodium azide to inhibit ATP synthesis via respiratory electron transport caused little change in these measurements, suggesting that chromatin diffusion does not require ATP hydrolysis. A similar analysis in Drosophila embryonic cells, using a mark in the chromatin generated by the localization of fluorescently labeled DNA topoisomerase II to a heterochromatic region of the X chromosome, gave similar values (D = 2 x 10-11 cm2/sec;. refereed research. How universal is the regulated localization of chromatin loci in interphase? Since the initial observations in Drosophila,. reports. The studies on bwD suggest that the insertion of DNA sequences that form heterochromatin near to a gene can influence the gene’s activity and location. What might these cis-acting sequences be? The recent development of synthetic compounds, such as oligomers of pyrrole and imidazole amino acids that bind to specific repetitive tracts of DNA with high affinity, has provided a biochemical tool for probing the effects of heterochromatin structure [25]. These compounds make heterochromatin more accessible to nucleases, presumably by making it more ‘open’, and they also show interesting effects in vivo. For example, Drosophila fed these compounds are viable, but show limited homeotic transformations. Moreover, the compounds also reverse the effects of heterochromatin silencing of both the white gene and the brown locus in bwD/+ flies [26]. It will be interesting to determine the effects of drugs affecting heterochromatin on the localization of specific gene loci. It is also possible that drugs targeted to other heterochromatic regions (such as telomeres) might be developed in future.. In summary, there is suggestive evidence that directed localization of chromatin loci is one mechanism for regulating gene expression within the nucleus. Targeted localization can be either permanent or transient. Although it is still unclear how general this process might be, it raises a number of important questions concerning the movement of chromatin domains in the nucleus. For example, do specific chromatin loci differ in their dynamic behaviour? And how rapidly can a chromatin locus move, and what factors mediate the movement of loci?. reviews. A clue to the mechanism of PEV came from studies on the Drosophila brown gene and a trans-inactivation phenomenon. In Drosophila, homologous chromosomes pair in somatic cells, allowing the localization of one homolog to affect the other. The brown-Dominant allele (bwD) contains a 1-2 Mb insertion of heterochromatin into the brown gene [21]. In a heterozygous animal bearing one wild-type and one bwD allele (bwD/+), transcription from the wild-type allele is repressed. In cells bearing two copies of the wildtype allele, FISH analysis showed that both loci were localized away from the nuclear periphery. By contrast, in bwD/+ flies, both alleles were clustered together on the nuclear periphery in the vicinity of heterochromatin [22,23]. These studies demonstrated that the location of a locus could move. Moreover, they suggest that one part of heterochromatin-mediated repression includes the recruitment of a locus to a heterochromatin zone of the nucleus. In this model, a gene proximal to heterochromatin in linear sequence is guided to a heterochromatic repressive zone, possibly by interactions between separate blocks of heterochromatin [24]. Furthermore, interactions of specific loci with heterochromatin can be transient.. targeted localization of heterochromatic sequences has been observed in fission yeast [27], mice, and humans, suggesting that it is does not depend on the somatic pairing of homologs that occurs in insects but is, in fact, a general phenomenon. In humans, the Ikaros DNA-binding protein is required for gene regulation during the development of B and T cells in the immune system [28]. Interestingly, Ikaros localizes to pericentric heterochromatin [29]. It has been proposed that it regulates gene activity by recruiting the loci it binds to the repressive environment of pericentric heterochromatin [30]. Ikaros localization depends on two Znfinger motifs, suggesting that it associates with pericentric heterochromatin by directly binding DNA [31]. Recent studies have identified a homolog of Ikaros, named Helios, that forms a heterodimer with Ikaros and also localizes to centric heterochromatin [32]. In addition, at least part of the cellular pool of Ikaros is complexed to NURD, a chromatinremodelling complex, suggesting that Ikaros function may also involve the remodelling of chromatin [33,34].. comment. cells, generating a variegated phenotype. The overall level of repression also depended on the proximity of white to heterochromatin: inversions that placed white close to heterochromatin repress more than those that put white farther away. This cis-inactivation might occur by the spreading of transcription-silencing factors from heterochromatin into the white gene [19]. Alternatively, the proximity to heterochromatin of white on the inverted X might expose white to a repressive environment, perhaps mediated by a cluster of heterochromatin [20]..

(4) 4 Genome Biology. Vol 2 No 3. Swedlow and Lamond. R = 0.9 mm) [36]. An analysis of chromatin movement in living human cells, using Cy3-dUTP-labeled DNA, found similar characteristics [38]. Taken together, these results suggest that bulk chromatin passively diffuses within the nucleus and that, in general, the volume within which it moves is quite limited. We note that these observations do not necessarily rule out the presence of specific paths that nuclear components could move along [39]; but a ‘tracklike’ mode of movement does not appear to be the dominant mechanism used by chromatin or other nuclear bodies so far examined (see below). It is instructive to consider the scale of these motions relative to free molecules and other nuclear components. Recent studies have used fluorescence recovery after photobleaching (FRAP), a technique in which fluorescence is initially bleached in a spot using a focused laser beam and the subsequent recovery of fluorescence is measured as a reporter of the diffusion of unbleached molecules into the bleached zone. The nuclear diffusion constants of GFP fused with the pre-mRNA splicing factor SF2/ASF, the chromatin-binding protein HMG-17, and the nucleolar protein fibrillarin, were measured in living cells using FRAP [40]. Diffusion constants for all three proteins were found to be approximately 10-9 cm2/sec, significantly faster than that measured for chromatin. A diffusion constant of 10-9 cm2/sec was also obtained for polyadenylated RNA in living cells [41] and again, no evidence was found for energy-dependent mobility. We have discussed (above) results indicating that repression of transcriptional activity can be correlated with the location of a chromatin locus. This highlights the importance of analysing the localization of a single locus during transcriptional activation. Recently, Tumbar and Belmont [42] have accomplished this in living cells using a stable cell line bearing 10-20 tandemly repeated copies of the dihydrofolate reductase gene DHFR fused to lacO and also expressing GFP-lacI or GFP-lacI fused to the acidic activation domain of VP16 (GFP-VP16-AAD). In cells expressing GFP-lacI, the DHFR-lacO repeat was seen localized to the nuclear periphery in around 50% of cells examined. In the presence of GFP-VP16-AAD, only about 30% of cells showed peripheral localization. These data suggest that transcriptional activation of a peripheral locus can change its position in the nucleus, at least in a proportion of cells. Taken together, these results provide a picture of a nucleus in which no components are immobile, but where proteins and other macromolecular complexes rapidly diffuse around, and perhaps within, slower moving, yet still mobile, chromatin. It should be borne in mind, however, that methods such as FRAP assay bulk populations of molecules and so may not identify small subpopulations of molecules with significantly different properties. Thus, even although passive diffusion may be the predominant mode of movement in the nucleus,. it cannot be excluded that some factors can move by energydependent mechanisms. If bulk chromatin movement is largely random and well confined, then two events are required to localize a chromatin locus specifically into a regulatory nuclear subcompartment. First, the locus must be liberated from a confined volume - the restriction to its mobility must be removed and second, a specificity mechanism must be available to target the locus to a specific subcompartment. In view of the large amount of data suggesting passive diffusion mechanisms, specificity probably comes from the binding of chromatin-associated factors, either to one another or to other nuclear components or receptors. This may be similar to the known mechanism of nuclear protein export that involves the selective transport of protein export cargoes to the nuclear pore complex, mediated by the nuclear export receptor Crm1 [43]. When might such movements be detectable? During S phase, all chromatin is disassembled and DNA is replicated and then reassembled into chromatin. This period of disassembly might be associated with increased chromatin dynamics and therefore might afford a chance for chromatin to redistribute within the nucleus. Indeed, in Drosophila the association of heterochromatic clusters has been shown to change during S phase [44]. In mammalian cells, analysis of the localization of an inserted late-replicating heterochromatic region showed movement from the nuclear periphery to the nuclear interior that correlated with late S phase [45]. A recent careful study in budding yeast revealed that late-firing replication origins were clustered on the nuclear periphery in G1 [46]. As cells advanced into S phase, late-firing origins lost this constraint and moved into the nuclear interior, apparently before the actual firing of the origin [46]. These data all show that large movements of specific chromatin loci can occur during S phase. It will be interesting therefore to extend the analysis of the dynamic properties of chromatin to see whether large-scale chromatin movements play an important role at other stages of the cell cycle.. Nuclear body dynamics As well as chromatin, there are different types of nuclear bodies, including nucleoli, speckles, Cajal bodies (formerly called coiled bodies; CBs) and PML bodies, each of which have distinct molecular components and are probably carrying out specific functions (Figure 2) [10,11]. Given the observed mobility of chromatin, it is not surprising to discover that these nuclear organelles are mobile as well. For example, time-lapse analysis following transient expression of a GFP fusion to the splicing factor ASF/SF2 showed that the speckled domains containing these factors were dynamic and could dramatically change shape [47,48]. Changes in the shape of speckled domains were inhibited by.

(5) http://genomebiology.com/2001/2/3/reviews/0002.5. comment reviews reports. In summary, recent studies have underlined the probable importance of regulated localization of specific chromatin loci and nuclear bodies as a way of modulating their function. In this review we have considered the recent data describing the dynamic behaviour of chromatin loci and nuclear bodies. We have discussed a general, two-step model that attempts to account for the movements of the large-scale nuclear structures that have been observed to date (Figure 3). A prediction of this model is that future analysis of the movements of chromatin loci and nuclear bodies will reveal marked changes either in diffusion rates, or in constrained volumes, as they undergo transitions from freely diffusing to tethered or constrained states, and vice versa. A major goal for future studies will be to understand the mechanisms involved in both moving and targeting large nuclear structures. It will be equally important to understand what mechanisms restrict their movement and tether them to specific locations. This will involve the establishment of better assay systems for detecting movement and the identification and characterization of nuclear factors that mediate targeted localization.. information. More recently, time-lapse movies of a stable HeLa cell line expressing GFP fused to the CB marker protein p80coilin have revealed that these can be remarkably dynamic structures. CBs traverse the nucleus and undergo both joining and splitting reactions [49]. CBs could also be detected moving to and from the periphery of nucleoli and rates of movement up to about 1 mm/min were recorded. CB movement has also been detected in HeLa cells expressing GFP fused to the nucleolar protein fibrillarin [49,50] and in the nuclei of plant cells expressing a GFP-fused snRNP protein [51]. The rate of CB movement changes markedly over time. CBs show transitions between making small movements that are apparently confined in a local subvolume and larger movements that involve their transiting more rapidly to more distant sites elsewhere in the nucleus. This behaviour of CBs is consistent with a two-step system for nuclear mobility, where constrained mobility is first relaxed, allowing the bodies to diffuse until they reach a new target or confined location. CBs have been seen to be transiently associated with many different transcriptional loci, suggesting that one mechanism for confining CB mobility may be. interactions. association with chromatin [52-56]. It is interesting to compare this putative mechanism with the proposed mode of chromatin targeting discussed above, and it will be important to test in future whether a two-step release and target model may provide a general way of organizing largescale nuclear structures.. refereed research. treatment of cells with a-amanitin, an inhibitor of transcription by RNA polymerase II. This suggests that dynamic changes in speckled domains were correlated with RNA polymerase II activity, and it was proposed that splicing factors can shuttle between speckles and sites of transcription [47,48].. deposited research. Figure 2 Chromatin and nuclear bodies. The micrograph shows the separate detection in the same HeLa cell nucleus of bulk chromatin (blue), the nucleolar protein fibrillarin (red), the RNA-splicing factor ASF/SF2 (green), and the Cajal body (formerly coiled body) autoantigen p80coilin (magenta). The right-hand panel shows an overlay of the four separate signals. Nuclear Cajal bodies contain both p80coilin and fibrillarin (arrows). ASF/SF2 localizes in nuclear speckles. Scale bar = 5 mm..

(6) 6 Genome Biology. Vol 2 No 3. Swedlow and Lamond. Figure 3 A simplified representation of a model for nuclear dynamics. (a) Chromatin loci and nuclear bodies are constrained within a nuclear subvolume, either by tethering to another structure or by physical boundaries to their movements. (b) Movement of a locus or nuclear body to a new location entails, first, relaxation of tethering or removal of the physical barrier. This is followed by transit to one or more new sites in the nucleus, and transit ends as a result of either binding via specific factors at a new location or encountering a new physical constraint. The new site can place the locus in either an active (c) or a repressive (heterochromatin) (d) domain. Current evidence suggests that movement between compartments is likely to be the result of passive diffusion, although at present we cannot exclude the possibility that energy-mediated movements might also occur. The further development of new and sensitive microscopy techniques to analyse the properties of fluorescently tagged fusion proteins will continue to provide insights into the dynamic properties of the nucleus in living cells. In our opinion, it will be of particular importance to focus such studies on the dynamics of single particles and specific chromosomal loci in living cells. Systems are now available to allow visualization of the events associated with the activation of a specific locus in a living cell [1,42]. While the focus of these studies so far has been on the events associated with transcriptional activation, it will be important to look in future also at specific repression events. We anticipate that quantitative analysis of nuclear dynamics will provide a much clearer picture of how structures are localized and moved around within the nucleus, and should improve our understanding of the effects of such movement on gene expression and replication.. Acknowledgements We thank Judith Sleeman and Barry Wong for providing Figures 1 and 2. We are grateful to Wendy Bickmore for helpful discussions. J.R.S. is a Career Development Fellow of the Wellcome Trust. A.I.L. is a Principal Fellow of the Wellcome Trust.. References 1. 2. 3. 4. 5.. Tsukamoto T, Hashiguchi N, Janicki SM, Tumbar T, Belmont AS, Spector DL: Visualization of gene activity in living cells. Nat Cell Biol 2000, 2:871-878. Tumbar T, Sudlow G, Belmont AS: Large-scale chromatin unfolding and remodeling induced by VP16 acidic activation domain. J Cell Biol 1999, 145:1341-1354. Cheung P, Allis CD, Sassone-Corsi P: Signaling to chromatin through histone modifications. Cell 2000, 103:263-271. Sudarsanam P, Winston F: The Swi/Snf family nucleosomeremodeling complexes and transcriptional control. Trends Genet 2000, 16:345-351. Pirrotta V: Polycombing the genome: PcG, trxG, and chromatin silencing. Cell 1998, 93:333-336..

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Results: Based on chi-square test results, the CHHF group showed a higher frequency of the following diseases: anaemia, hypotension, chronic gastritis, reflux oesophagitis, chronic

Abstract Background: On account of deterioration of chronic heart failure CHF and extensive exploration of Chinese herbal injections CHIs, we performed a network meta-analysis to

Abbreviations HR-QOL: Health-Related Quality of Life; IIQ: Incontinence Impact Questionnaire; LUTS: Lower Urinary Tract Symptoms; OAB: Overactive Bladder; OAB-SF: Overactive Bladder

jacquemontiana leaf extract except aqueous fraction have strong antibacterial potential, especially against all MDR strains tested and three fractions PJM, PJE and PJB have strong