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Strategies for the Reconstitution and Purification of Haloarchael Protein RadA

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Academic year: 2020

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Figure

Fig.absorbance as compared to the proteins visualized
Figure 3. SDS-PAGE (Left) and DNA agarose gel (Right) analysisof soluble E. coli cell extract containing RadA precipitated withpolyethylenimine.M=Marker(kDa),1=RadApre-induction,2=post-4 h induction, 3=RadA incubated with PEI, 4=Supernatantof PEI-precipitated samples, 5=pellets after centrifugation of PEIprecipitation
Figure 4. Time course for benzonase incubation with solublelysate containing RadA in 500 mM NaCl
Figure 6. SEC of RadA after refolding.(A) A chromatogram showing the elution profile of RadA using a26/60 Superdex 200 SEC column (B) & (C) SDS-PAGE andagarose gel analysis of the obtained fractions (1-6 and 10-11)corresponding to the UV absorbance peak obtained at 110-160mL and 200-210 mL, respectively.

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