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Stainless Steel

Dissolved Oxygen Electrodes

DISSOLVED OXYGEN ELECTRODES _________________________________________________

The steam sterilizable family of dissolved oxygen electrodes from the pHoenix Electrode Company is

specifi-cally designed for fermentation where it is necessary to subject electrodes to repeated steam sterilization.

Dissolved oxygen electrodes are available in both polarographic and galvanic styles to fulfill all requirements.

pHoenix O

2

electrodes are compatible with most common O

2

amplifiers found in fermentation. Polarographic

electrodes are designed to be used with pHoenix Electrode Company, Ingold IL, Cole-Parmer and Applikon O

2

amplifiers, or with other amplifiers that are designed to be used with Ingold dissolved oxygen electrodes.

Galvanic electrodes are designed to be used with pHoenix Electrode Company, New Brunswick Scientific, LSL

Biolafitte and Applikon O

2

amplifiers.

The DO probes are available for laboratory fermentation where sterilization takes place in a separate autoclave

or industrial applications where sterilization takes place in-situ.

The easily replaced electrode membrane cartridge is a unique feature of the pHoenix polarographic and NBS

galvanic dissolved oxygen electrodes. The membrane is of a special construction - a silicone and teglon

composite, reinforced with stainless steel mesh. This unique membrane is very rugged and allows for

re-peated sterilization without damage, allowing ot to withstand severe conditions. The electrode operates

with-out maintenance for 5 to 10 sterilizations, fermentation cycles or, in terms of time, for 6 to 8 weeks. The

pHoenix Biolafitte type galvanic dissolved oxygen electrode features easily replaceable FEP teflon

mem-branes.

The polarographic dissolved oxygen electrodes are available with or without automatic temperature

compensa-tion. They are designed to match the Ingold IL oxygen amplifier. Temperature compensation is not normally

required when calibration is made at the same temperature as the controlled fermentation temperature with no

variation.

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SPECIFICATIONS

A

B

C

D

E

F

G

H

I

A1

A2

A3

A4

A5

A6

OXYGEN ELECTRODE

ASSEMBLY

A. Detachable Lead

B. O-ring Seal

C. 316L Stainless

steel body

D. O-ring

E. Anode

F. Membrane Cover

G. Replacement

Membrane Cartridge

H. O-ring

I. Cathode

MOUNTINGS

DESCRIPTION

19mm socket

flange

M18 x 1.5

3/4” NPT

3/8” NPT

25mm socket

flange

No Thread

PG 13.5

BODY

DIAMETER

19 mm

16 mm

19 mm

14 mm

19 mm

12 mm

12 mm

TYPE

07

05

03

01

08

10

11

FIGURE

A1

A2

A3

A4

A5

A6

A6

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SPECIFICATIONS _________________________________________________________________

TYPE

CATHODE

ANODE

RESPONSE

STABILITY

1

TEMPERATURE

COMPENSATION

TEMPERATURE

RANGE

STERILIZATION

TEMPERATURE

RESIDUAL

SIGNAL

OUTPUT

2

025IP25

Polarographic

Pt

Ag/AgCl

98% 60 Seconds

Better Than 2%

50 nA

025IP26

Polarographic

Pt

Ag/AgCl

98% 60 Seconds

Better Than 2%

50 nA

025IP27

Polarographic

Pt

Ag/AgCl

98% 60 Seconds

Better Than 2%

50 nA

025BG25

Galvanicic

Ag

Pb

98% 60 Seconds

Better Than 2%

35mV + 10mV

025IP25

Polarographic

Pt

Ag/AgCl

98% 60 Seconds

Better Than 2%

50 nA

NO

YES

YES

NO

NO

0-80°C

0-80°C

0-80°C

0-80°C

0-80°C

130°C

130°C

130°C

130°C

130°C

Less Than 1 nA

Less Than 1 nA

Less Than 1 nA

0.5 - 1 mV

Less Than 0.4 µA

1

= Constant Temperature

2

= Medium Saturated With Air

ORDERING INFORMATION _________________________________________________________

025IP 025BG

025NG

Sensor Type

Polarographic Stainless Steel Galvanic, LSL Biolafitte pHoenix and Applikon Compatible, Stainless Steel Galvanic, New Brunswick Scientific Compatible, Stainless Steel 25 26 27 Temperature Compensation No Temperature Compensation Temperature Compensated Ingold Amplifier

Temperature Compensated pHoenix Electrode Company, Cole-Parmer, and B&C Electronics 01 03 05 07 08 10 11 Mounting

3/8” NPT Male Thread (For NBS Headplate), 14 mm Body Diameter. 3/4” NPT Male Thread, 19 mm Body Diameter.

M18 x 1.5 Male Thread (For LSL Biolafitte and Applikon Headplate), 16 mm Diameter.

19 mm Socket Flange, 19 mm Body Diameter. 25 mm Socket Flange, 19 mm Body Diameter.

12 mm Body Diameter Straight Body. 12 mm Body Diameter with

PG 13.5 Thread.

025IP - 25 - 150 - 05

70 mm 120 mm 150 mm 220 mm 320 mm 420 mm XXX mm “H” Length Polarographic 08 Mounting

10 and 11 Mountings 03, 05, 07 and 08 Mountings

03, 05, 07, 08, 10 and 11 Mountings

03, 05, 07, 08, 10 and 11 Mountings

05, 07, and 08 Mountings Special Order “H” Length

70 mm 150 mm 220 mm 320 mm 420 mm 440 mm Galvanic (LSL Biolafitte Fermentor) 08 Mounting

05, 08, 10 and 11 Mountings

05, 10 and 11 Mountings 05, 10 and 11 Mountings 05 Mounting 05 Mounting 127 mm 186 mm 229 mm 308 mm 355 mm 360 mm 432 mm 559 mm 711 mm Galvanic (NBS Fermentor)

01, 08, 10 and 11 Mountings 01, 08, 10 and 11 Mountings 01, 08, 10 and 11 Mountings 01 and 08 Mountings 01 and 08 Mountings 01 and 08 Mountings 01 and 08 Mountings 01 and 08 Mountings 01 and 08 Mountings

“H” Length (Immersion length is the distance from the point of penetration inside the vessel to the tip of the probe.) EXAMPLE: 025IP25-150-05

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MEMBRANE SERVICE KITS AND MEMBRANES ________________________________________

POLAROGRAPHIC TYPES

M0500P1-K1

M0500P1-K2

M0500P1-K5

M0500P1-M1

M0500P2-M2 M0500P2-M6 R001068

Membrane Service Kit for Polarographic O2 electrodes with 3/4” NPT, 19 mm or 25 mm mounting with 19 mm O.D. body diameter. Consists of replacement O-rings, 3 replaceable membrane cartridges, tool, and a 125 mL bottle of electrolyte.

Membrane Service Kit for Polarographic O2 electrodes with M18 x 1.5 mounting with 16 mm O.D. body diameter. Consists of replacement O-rings, 3 replaceable membrane cartridges, tool, and a 125 mL bottle of electrolyte.

Membrane Service Kit for Polarographic O2 electrodes with PG 13.5 or 12 mm mounting with 12 mm O.D. body diameter. Consists of replacement O-rings, 3 replaceable membrane cartridges, tool, and a 125 mL bottle of electrolyte.

Replacement Membrane consists of one membrane cartridge and replacement O-rings for 3/4” NPT, 19 mm or 25 mm mounting, 19 mm O.D. body diameter.

Replacement Membrane consists of one membrane cartridge and replacement O-rings for M18 x 1.5 mounting, 16 mm O.D. body diameter.

Replacement Membrane consists of one membrane cartridge and replacement O-rings for PG 13.5 or 12 mm mounting, 12 mm O.D. body diameter.

Polarographic O2 electrode filling solution, 125 mL

GALVANIC TYPES

Membrane Service Kit for Galvanic O2 electrodes with M18 x 1.5, 19 mm or 25 mm socket with 16 mm O.D. body diameter. Consists of 10 membranes (5 each of 1 and 2 mil), replacement O-rings, tool, and a 125 mL bottle of electrolyte.

Membrane Service Kit for Galvanic O2 electrodes with 3/8” NPT mounting with 14 mm O.D. body diameter. Consists of replacement O-rings, 3 replaceable membrane cartridges, tool, and a 125 mL bottle of electrolyte.

Membrane Service Kit for Galvanic O2 electrodes with PG 13.5 or 12 mm mounting with 12 mm O.D. body diameter. Consists of replacement O-rings, 3 replaceable membrane cartridges, tool, and a 125 mL bottle of electrolyte.

Replacement Membrane consists of 10 membranes 1 mil thick and replacement O-rings for M18 x 1.5, 19 mm and 25 mm mountings, 16 mm O.D. body diameter.

Replacement Membrane consists of 10 membranes 2 mil thick and replacement O-rings for M18 x 1.5, 19 mm and 25 mm mountings, 16 mm O.D. body diameter.

Replacement Membrane consists of one membrane cartridge and replacement O-rings for 3/8” NPT mounting, 14 mm O.D. body diameter.

Replacement Membrane consists of one membrane cartridge and replacement O-rings for PG 13.5 or 12 mm mounting, 12 mm O.D. body diameter.

Galvanic O2 electrode filling solution, 125 mL M050BG1-K3

M050NG1-K4

M050NG1-K1

M050BG2-M3 M050BG2-M5 M050NG2-M4 M050NG1-M1 R001069

CABLES ___________________________________________________________________________

C1 TYPE CABLES

For non-temperature compensated O2 electrodes, used with both polarographic and galvanic O2 electrodes. Standard 3 meter lead. BNC Connector

DIN Connector for use with Ingold pO2 amplifier

Dual Pin Connector for use with NBS Galvanic pO2 amplifier Banana Plugs for use with LSL Biolafitte pO2 amplifier

No Connector for pHoenix, Applikon, and Cole-Parmer pO2 amplifiers or for use when user supplies connector

Custom cable with choice of plug, lemo connector. Specify size and pin nimber

Pin Connector, for use with 025NG15 and 027NG15 Galvanic O2 electrode. Cable features a load resistor for direct connection to a recorder with mV input. (Approx. 40 mV saturated air / water)

Amphenol4-Pin Electrode head connector for use with NBS pO2 amplifier

Amphenol detachable lead to Amp 4-Pin connector for use with NBS pO2 amplifier 9001091-010B

9001091-010I 9001091-010D 9001091-010U 9001091-010T 9001091-010CC 9001093-010D 9001096-010D 9001096-003

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C2 TYPE CABLES

For polarographic temperature compensated O2 electrodes. Standard 3 meter lead. DIN Connector + pins for use with Ingold pO2 amplifier

No Connector for pHoenix, Applikon and Cole-Parmer pO2 amplifiers, or for use when user supplies connector

Custom cable with choice of plug type, lemo connector. Specify plug size and pin number

Amphenol 4-Pin Electrode head connector to Amp 4-Pin Connector. For polarographic electrodes, NBS

Note: Longer lead lengths are available on special order.

9001092-010I 9001092-010T 9001092-010CC 9001097-003

6103 Glenmont • Houston, Texas 77081 • 800/522-7920 • 713/772-6666 • Fax 713/772-4671

References

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