ER-500

SINNOWA@

i+-r'N.F

it+

Table

of Contents

1.1 1.2 1.3 1.4 6 TEST

I

-1-7 PATIENT INFORMATION.

...29

I

-2-1

lntroduction

1.1

Main Features

The features of the filters are shown in table 1 :

Table

I

Feature Technical Parameter

Wavelength Accu

racy

( _{nm )}

_t2.0

Half-Width ( _{nm ) <10} Transmission Peak

Ratio

(%)

>35

-O.D.

Accuracy:<

t0.01A

or

¡1%

.

-

Linearity: r>0.999 .

-O.D.

Reproducibility:<0.5% .

-O.D.

Stability:Drift Error<0.005A in 1Omins; Drift Error<0.005A when the supply voltage swings

t10%

.

1,2

Specifications

-Graphical

operating interface, menu designed for convenient use.

-500

programmable analysis items.

Four

measure

method

:

Single

wavelength/Dual

wavelength/Fixed

time/Kinetics.

-Eleven

analysis means,

ABS

/

Cut-Off

/

Single

point

regression

/

Multipoint

regression

/

Linear

regression

/

ABs%/Exponential

Regresssion

/

Logarithm

Regression

/

Dual

Logarithm Regression

_/

Logit Regression

/

Polynomial Regression.

-

Performing 12 different tests

just in

one

plate. Flexible Plate

Position with

Blanks,Reference,standards.Samples and QC in one screen.

-

Proven optical system

with

8

channels optical

fiber

scanning,

auto

plate

centering system positions

the

center of

the

cell accurately.Get reading result in

just

10 seconds.

-

Printing patient reports with the thermal printer.

-

Easy connect to PC,comprehensive functions with PC software.

1.3

External

Features

Top View as figure 1:

Figure

I

O

Power indicator light

@ LCO and Touch screen

Figure 2

O

Power ON/OFF switch

@

Power cord receptacle

@

Fuses

@

Gemel

@

RS-232 serial interface

@

Parallel printer interface

@

USB interface

@

PS/2 mouse and keyboard interface

@

Serial Number Label

k,

-2-1.4 Specifications

o

Place the instrument _{on a clean, sturdy table or bench.lt is}

important

_to

keep

the

instrument

_in

a

_{ctean, relatively}

_dust

_free

_environment

to

ensure maximum performance.

I

Weight: Outline Dimension:

460mm

(L)

x360mm

(W)

x21Omm

(H)

AC(220L22)V

,

_(50t1)Hz

F2AL250V 2-9Sx20 mm

Temperature:

*s-+.40c;

_{Rerative humidity}

_:

_{2}o/o-gooil; Atmospheric}

pressure:

_860-1060hPa

Storage temperature:

-10c-50c

Light Source: _{Tungsten Halogen Lamp,}

_OSRAM64607,

BV/SOW

Wavelength:

_405nm,

_450nm

_,

_4g2nm,

_630nm lndication

r3flge:

Continuous

_mode<10s,

_{Stepping mode<20s}

ARM core series Programmabl

e

ltems: lnterface:

"r'.

usB..

Mouse and keyboard interface

Display: _{320x240 LCD}

lnput Mode, Touch Screen _{,Touch pen}

Memory Capability:

2000

Plates

( _{96 holes}

)

_{test data}

2

lnstrument

_Set-up

2.1

lnitial

Start-up

-3-o

Connect

the

power cord to

the

back of

the

instrument.Before connecting the

instrument

to

the

main electrical supply, check

that the

AC

voltage

is

appropriate for the instrument.

3

lnput operation

3.1

Touch Screen and

Pen

ln

the

Microplate

Reader, the

pen

is

used

to

click on the area of the visual

screen. The

valid

method

of

input operation is

the

single click,

which

means to touch the screen by the pen or the finger and then to remove immediately.

3.2

Digit

soft

keyboard

!t is used

to

input the integer (like the time,date)and the decimal fraction(like

the concentration) as Figure 3.

Pletse inFl¡t Lll[ cEutra=t ¿res ü - I5

-trtrtrr

trtrtrr

trtrtr8

trtrtr8

Figure 3

3.3 Gharacter

soft

keYboard

Keybord

is

shown

as

Figure

4. lt

is

used

to

input

the

character string. The

"<-"

key

is

used

to

delete

the

character, and

the

"CAP"

key is

used

to

shift the uppercase and the lowercase of the character.

FLease input l:,:spltal t:alte Flease irrput hnspittl r:aue Hax. tenr¡tlr :rrJ llax. LEngth f,ü

tEE-E-EtrtrtrtrEitrtrEltrEltrtrtr

Etrtrtrtrtrtrtrgt]_trtrtrtr

Etrtrtrtrtrtrtrtrtrtrtrtr

Etrtrtrtrtr_trElIl_trEgtr

Etrtrsssstrtrtrtrtr

ffitrtrtrtrtr@

l=,,-ltrtrtrtrtrtrtrtrtrtr

I il,1-

]trtrtrfltr@trEtrtr

fqmn-l-l@@tE

EEtrl-l@@tI

Figure 4

-4-3.4 Date/time

_soft

_keyboard

It is

used

to

input

the

date and

the

time

as

Figure

S. The

format

is

Year-Month-Day and the "Today" is used to _{input the date of today quickly.}

PLease input drte

2000-rl1-rl1 309S_12_3I

r

trtrtrr

trtrtrEq

trtrtrE

trl-r_",

lffi

Figure 5

3.5

Formula

soft

keyboard

It is

used

to

_{input the formuta as figure}

_6.

_{There are}

_four

_{N(Negative).. four}

P(Positive)'.

eight

D(Standard)..

MIN(get

the

minimum value).. MAX(get

the

l

i

maximum value) and the common operators.

Please 1r:put tor¡luta L

Ilax. Iengrh S0

EEEEEE4ETE@

EEEEEEEE@

EEEEEEEE

I * I

lil-*l

fofr-l

IFI

Figure 6

4

Start-up

4.1

_{Procedure of start-up}

Turn

on the

power switch

on the

_{rear panel. After about three seconds, the} instrument

will

perform

an

initial

self

diagnosis

that

requires about

one

minute.

Please

wait

ten

minutes

_for

_the

instrument

_to

_warm

_up(reach

_thermal

equilibrium)before reading plates.

The _{procedure of start-up inctudes:}

1)

Load the program;

I

-5-2).

Access the user data;

3)

Wait for the stable light of the Iamp. It is necessary to wait about five minutes

for the

stabilization

of the

lamp before the

first reading. (The

result

of

O.D.

may be not accurate if the lamp is not stable. )

4)

Self diagnosis of the optical and

the

mechanical system .The filter wheel will turn around and the plate will reciprocate once.

4,2 Main Menu

The screen displays the main menu after the procedure of start-up. The main

menu has six

different modes: Patient lnformation, Test, Report,

System,

ltem Setup and Search as Figure 7.

JB

lEl

Feport

H,q

Search

IE

Info Test

I-F\

u

atient

tr

_--:-=

_E SYsteü

5

ltem Setup

Choose

the

ltem

setting

window as Figure 8:

¡con

The user could set 500 different items at most.

5,1 Measure

method

The reader has four measure methods.

&

w

Iten

Setup

Figure 7

in

the

main menu and

go

the

item

]D Iteu I,¡EDe FuII I,laue anEIYS].E

I IlBsAg iepatitis B Eut-0ff

Z IlEslb lepEtitis E Eut-0ft

3 HtseAg lepatitis E [ut-0ff 4 IlBelb lEp8titiE E f,ut-0ff 5 IlEcÁh lep8titis E Cut-0ff

Page Up Page Dorln ller¡ llodifl DeIete Feturn

Figure 8

-6-5.1.1

Single wavelength

The _{method uses one wavelength to measure the}

_oD.

5.1.2

Dual

wavelength

The method uses two wavelengths

to

measure

the

OD.

The

result

of

OD is

the difference of the main wavelength and the reference wavelength.

5.1.3

Fixed

time

A

first

absorbance measurement

_is

_made

_for

_each

_sample

_after

_a programmed incubation period, and a second measurement is performed after a

defined interval. The fixed time method should use the single regression analysis method.

5.1,4

Kinetics

A

first

measurement

_of

_absorbance

_is

_{made against}

_the

_{BLANK after}

a

programmed incubation

period.

Next,

new

measurements

are

made

at

defined

intervals

of

time. The absorbance

is

calculated

as the

mean

of the

increase (or

decrease)

in

absorbance

per

minute.

The

_{kinetics method should use}

_only

_one

wavelength.

5.2

Analysis

method

There are eleven analysis methods, including the comprehensive qualitative

and

quantitative

_{data-evaluation}

_functions

_with

_cut-offs,

curve-fits

and

tra nsformation form u las.

5.2.1 O.D.

mode

Measure the O.D. of the plate cell direcfly.

5.2.2

Qualitative(Gut-Off)

_mode

cut-off

limit formula

z

cov ₌

x

x NC

+y

x

pc

+ Fac

NC is the O.D.

of

the

negative reference

_{materiat, PC is the O.D.}

_of

_the

positive reference

material,X,Y,

Fac is the coefficient, which is set in the reagent

instruction.All

_{the other}

_kind

_of

_{qualitative formula}

_can

_{convert into}

this

formula

format.For example:

if

sample oD/Negative Ref.oD>2.1

_is

_the

positive,then

X=2.1

,

Y=0,

Fac=O.

The

ratio

of

the

sample O.D.

and

the

Cov

is

often used

to

define

the qualitative result,and the unit is s/co.The normal s/co threshold is 1.

5.2,3

_Quantitative

_mode

1)

Single point regression

mode:

ore

standard

needed,

use the origin and the

n

t-2)

standard O.D. to fit the curve as Figure

9.

(X:concentrate,

Y:O.D.)

Figure9

Multi point

regression:

Two to eight standards

needed,

use all the standards O.D. to fit the curve as Figure 10:

C

Figure

l0

or

decrease),the

result

is

error

as

lf

the

curve

is

not

Figure 11 :

monotony(increase

o.D.

3)

Figure l1

Linear regression: Two to eight standards

needed,

use these standards to fit the regression curve

as

Y = kX + ó in Figure 12:

Figure 12

-8-I

4)

Exponential

Regression

:

_Two

_to

_eight

_standards

_needed

_,

use

these

standards

_{to fit the}

_{regression curye}

_as

_y

= kebx,all

the

_{standard O.D. must}

be positive number.

5)

Logarithm

Regression'

Two to eight standards

_needed,

_{use these standards}

to

fit

the

regression

_curye

_as

_Y

_-kLnx+b,all

_{the standard O.D. must}

be

positive _number.

6)

_{Dual Logarithm Regression, Two}

_to

_{eight standards needed, use}

these

standards

to fit

the

regression

curve

as

y:lg$Lnx+b),all

_the

_standard

O.D.

must

be

positive

number.

_lf

_setting

_the

_{concentration}

_as

x'

=

Lnx ,Y'

=

Lny,then

the _{regression formula turns}

_to

y, _{_}

_k,x,+b,

_.

7)

log-logit Regression: _{Two to eight standards}

_needed,

_{use these standards}

to

fit the _{regression curve as Log-Logit method.}

The_ _{abscissa of Log-logit curve}

is Concentrate, the ordinate

is

¡-!-

oD

^

concentrateofthestan,:::";:',,?",¡t¡l;m';:il:::,Hi:,"

B)

Polynomial _{Regression, Two to eight standards needed,}

use these standards to fit the regression _curye

_as

_Y

-

kxb,

all the standard

o.D.

must be positive

number.

5.3

Add

New ltem

choose

the

_{ltem setup icon}

_in

_the

_main

_menu

window _{as Figure 13.}

and

go

into

the

item

list

fte¡l rrane tlTl,lL

Fr-¡lt- llsnre

_l-.Trr.rr

I

Feagerrt

_{l@-rrrll:tai,'ra'elength}

rEsr

I,re1:Iro,:fEffiEl

_l.I.rTEl

Arrar-s=is

_lffiTTiEEEl

S :iirJ'¡1e Salr _f,, D,:trhte

Ersnl: Ilu*.|ffi| _:i¡rr

ff tlnluun r¡ir:u::

I'ro.

l..Tl

Fer.

rrealffitrl - Iffil

I

r.¡.-*

I

_f-

,-,,,

I

I

E*

I Figure 13

Select"New"key _{to add new item.}

The _{parameter of the item setup:}

o

ltem name: ltem brief name, string including _{twenty characters at most, blank} unallowed;

}

-9-o

Full name: ltem full

name,

string

including

thirty

characters

at

most,

blank allowed.

o

Reagent: Reagent name,

string

including

twenty

characters

at

most,

blank allowed.

.

Test Method: Select the test method from the four methods.

o

Main/Second Wavelength: select

from the

wavelength

list,

and

the

second

wavelength should be different with the main wavelength;

o

Analysis: Analysis method, selected from the list of methods.

.

Single Sam/Double Sam/Column minus: the

type of the

sample,

the

default

setting

is

single sample,

the

double sample means double sample

for

each

patient,

the

column minus method is

a

specific method,

which

uses

the

second

column minus the first column as the sample value.

o

Blank

Max.'

Define

the

max. value

of the

reagent

blank.For example,the

reagent needs

the

blank less than 0.05,then

the

upper blank must be 0.05.1f the

blank

O.D. is

larger than

the

upper blank, "Too Iarge

OD

blank"will appear and

the maximum blank is used as the blank automaticly.

o

Sort No.: Define the sort of

the

item in

the

patient report. The small No. is in

the front of the list. The default value is 100.

o

Ref.

Area:

set the normal

area

of the item.The area

is

used in the

report

only.The area value don't

take

part in the formula calculating. The default value

of

the area

is

0.000

N

0.000, and

the

column

will

not

print

if the

area

is

0.000

_-

0.000.

.

Analysis Means:

choose

the

analysis means

from the

list.According

to

the different

means,

the screen will appear different windows.

o

Wait

time:

used in the Fixed time method and Kinetics method, the range of the value is 10

to

120;

o

Test

times:

Used in the Kinetics method, the range of the value is 2

to

10. If the analysis method is Cut-Off, click "Next" to view the menu as Figure 14:

.

Negative/Positive Ref

Area'

The valid

reference

area of the

Negative and

Positive,

the default value is

0.000-4.000;

o

Negative/Positive

1-4:

User

can see the OD of the

Negative/Positive after test, or set the OD directly without test.

-10-tr.trüpl

ffi

lresari,e

_trEI

_l4Jrtrúl

Pnsitir¡e Fef Area

f nrrtrü

_|

_trEI

F,rsirirE

_trEl

_{I r.ltrü}

| Iiegative I I'IEgEI:iTJE T IJegatirre lr I,legatilre 4 Figure 14 Click "Next":

o

Qualitative Group

Name,

User can define the _{name or signal of every group;}

Click the label "Group

1"-"Group

5" to set the predefined

name,

which will

shift between the _{"Negative,Weak negative,Neutral,Weak positive,positive,,;}

o

Formula(Cut-Off

_value):

_{User can define the C.O. formula or value of} every group, _{four formulas at most;}

The relation of the Cut-Off

value..

OD value and qualitative is as Figure 15:

l+

Cut-Off value _OD

Figure 15

From

the

manual

of the

EL¡SA k¡t,

the formula

_{of the}

_{cut off}

_is

,,cut

_off

value=Negat¡ve

x

_2.1"

,

_so

_the

_{formula should}

_be

_2.1.NC1

_;

_lf

_there

are comments like"Minimum Negative OD = 0.05

",

the meaning of the comments is

to get the large value of the NC1 and

0.05,

so the final

c.o.

formula is:

2.1.MAX(NC1 ,0.05)

This is the

formula

of the Group

1

.

There

are four

_formutas

_{at all. And}

_the

value

of the

formula must

be

"Form ula

4

>Formula

_3>

_Formula

_2>

_{Formula 1,}

.

The five groups have four formulas.

lf the item has more _{formulas, the CO vatue in S/CO could be selected from} the four formulas. The default vatue is 1 .

lf the formula is longer than the viewing area, just _{click the formula before the}

group label to show the full formula in the last line as Figure 16.

I,Jer¡rtlue Fef Ire¡

Fositl',:e l_ P¡sitire X Pnsitire f, Fr:sitir,'e 4 | ürrstl lr-rrrtrül I ú. orrr _I

I-ffi|

lP=*j",,=l

f*E [

ü,-

If

E*

|

Iffil

l'trrxrtr I

Iffit

| ür,ütr _|

ü

llr.¡af itative _FnruuLa

lroup

llGiIll+lffil

ü.2+1I¡I+FI

{

L'irrrup ¿l'iiE'?Il+

I-l

Erc,u¡r

i[-l+

l-l

Group

4l-l', I-l

rirnup

rl-l

Select the f'lrüu1u[ u= f,Ü rralue

llllclr fsrlüu1a 1¡he1 trErI dispra'j t¡h':'Le tnrrür'¡1e

fp.",,i",-l

t

n-*

I

I

úk

I

I

E="

I

Figure 16

lf the ana¡ys¡s method is quantitative method, click "Next" to view the menu:

o

STD numbers: define the numbers of the STD.

.

Dilute

Multiple'

define

the dilute

multiple

of the

sample, and

the

calculated

value should multiply the dilute multiple.

o

Unit: Choose unit from the 24 common units in the list.

o

Dual STD:

lf

check

the

dual

STD,all

the

standards

in

the

plate

will

be

positioned double times.

.

ABS

percent:

check

the

box

to

use

_"D^

+"100/o

as the

ordinate

of

the

standard

graph, the

D0 is

the

reference stañdard

of

100%

O.D., and

the

D0

needs no concentrate.

lf the

item se¡ects

the ABS

percent,

the first

standard is

D0

when

position

the

plate.

The dual

Iogarithm regression cannot use the ABS percent because of the ordinate is in logarithm.

.

STD

concentrate

table:

Display

and

select

the STD

concentrate. Attention:

AII the STD concentrate should increase.

.

Modify concentration: Modify the selected concentrat¡on as Figure 17.

sTD

uuu

_ft f,,i1r.¡te I,lr-r1tip1e _E Uni-t

I

r:r.rrl

[.+rs

5TD Percent: Hod j-t1 llsncenl:ration

,5TD f,oncentratiun shor.lld increast

lp'-,j--llil.=*l¡

n-

IIB*

I

Figure 17

Click "Next" to set the qualitative group and formula as the Cut-Off item.

Click "Next"

to

set

the

Quality Control.

The

menu

of QC is

demonstrated in the

ID Il¡ncerrtration I I. tltltl Z, z. tltltl 3. t-il:il:l 4 4 . r_il_r L-l E J 5. IUr]

-12-next sect¡on.

After

setting

all the

parameters,

_click

"OK"

_to

_save

_the

_{item. Attention: The}

same name of the item is not allowed.

5.4

_Quality

_{Gontrol Settting}

The

qualitative

_and

quantitative

_{items both could set two}

_eC

_materials

as Figure 18. @uc r

_Enc

¿ QHnoron X- 5Il

fi

t-'I ff fnstart n¡eans QHnorrrr H_ SD O L-J 0 lrrstBnt nJEans Ilodify 5I Tabte l"es

I

E="-l

Earch

r,r".lffi'l

Eatch l¡o.

[:ffi|

ruerase

_f

r-ál

plttutti-ruree

_I

l,]rrIt1-rü

lP.*i",,=l

I *¡

Figure

l8

o

Batch

No.:

The

batch number

_{of the QC materials,}

_{different batch QC}

materials should have different

eC

data;

o

Average:

_{The average value of the}

_ec

_{materials(F);}

o

sD'

The _{standard deviation of the}

_ec

_{materials (.gD).}

The

routine

_{quality control}

_in

_room

_includes

_the

_routine

conditions variance-knownvalues (

_RCVK)

and routine conditions variance-unknown values

(RCVU).

For RCVK,

select"Known

₇

.

SD", ohd input

the

7

and

5D

in the manual of the QC materials.

For

RCVU,

_{select L-J}

_or

_{instant means.}

_The

_{Levey-Jennings}

rule

uses the front 20 values to

calculate

₇

. SD, and makes the

eC

graph

_from

_{the 21rt value.}

The

_{instant means uses}

_the

_{Sl table,}

_calculates

_x.,sD

and

makes

_{the eC}

graph from the 3'd value.

The limit of all these QC

methods

is

i

tzs.

with

the

development

of

the

computer, there are

more

and

more efficient

control

methods.

The

instrument

integrates

_a

_{common multi-rules control method:}

_westgard

Mutti-rules.

Here

is

the routine of

westgard

Multi-rules as Figure 1g:

-13-B

t

Out

of

control Figure 19

I,

t,

I,

I,

I,

:l'

ffir

In

control

5.5 Mod¡fy

the

item

Select

an

item from

the

list and click the "Modify" key

to

modify

the

item

setting.

The Cut-Off value will be cleared if the Cut-Off item is modified.

The

quantitative curve

and

the

STD

concentrate

will be

cleared

¡f

the quantitative item is modified.

5.6 Delete

the

item

Select an item from the list and click the "Delete" key to delete the item. lf the

"ok" key in the confirm window is clicked,the item

will

be delete completely.lf the "Cancel" key in the confirm window is clicked,the deleting will be cancelled.

6

Test

6.1 Test

setting

Click

the

Test icon

in

the

main menu

and

go

into

the

plate

reading

mode.select the parameter from the window to modify the setting as Figure 20.

It""d s.-""ilI IE="J.=rr-i1 | Sterltri]. HÉDe:

PI€te Setr¡I]

DiEectiorr QIIorizonta]- OVerti,=EI I{ovj-r¡q Hode Q Corrtinuous Q Steppj-ng shÉkir¡g Hode sharr:.net s¡:eed ls]", -E 5hÉk:-rrg Tiue _{lü s=*o"d E}

I * I I

"="1

Figure20

-14-T

o

Load

stencil:

toad the preset _{stencil of position;}

o

Plate

Direction:

_{serect Horizontar or Verticar position;}

o

Moving

Mode:

select the plate _{continuous moving or stepping}

moving;

'

Shaking

_speed:

_{select the}

_slow.,

_{medium or}

fast _{shaking speed;}

o

Shaking

_time:

_{select the time of shaking plate,}

user-defined _{area is from}

10

to

180 second.

6.2 Plate Hole

Table 3

(_-

o

The direction of the plate,s moving is

6.3 Stenc¡l

Click the _{"Load Stencil" Button to go to the Plate Load tnterface}

as Figur e 21 . t -".r" ,r, I

F*-

t,-,;l

I-l

I

--.1

Figure 21

select an

_{available stencil in}

_the

_{list and click}

_ok

_to

toad

the

position

_of

_the

stencil'

All

_{informations including}

_{the direction, moving mode, shaking}

speed, shaking time, the _{item and the type of each hole are stored}

in the stencit.

6.4 Hole

Position

User

can

position

_the

_sampre(s),

_{Brank(B), Reference}

(N, p), standard(D),Quality

_control(oc)

_{in any hole of the}

standard

_{96 holes plate}

_as

Figure 22.

P.l-Ei:E LoEd

III 5tr=I.I':i1 f t: e¡l Hrile ! 1 2 3 _{4 5 6} 7 8 9 10 11 ₁₂ A A1 A2 A3 A4 A5 A6 A7 87 A8 B8 A9 B9

410

810

All

412 B B1 82 B3 B4 B5 B6 C6 811 H^12 C c1

c2

C3 C4 C5

_c7

_{CB C9}

_c10

_c11

_c12

D D1 _{D2 D3 D4 D5 D6} D7 DB D9 D10 D11 D12 E E1 _{E2 E3 E4 E5 E6}

E7 EB E9 E10 E11 E12

F F1 F2 F3 F4 F5 F6 F7 F8 F9 F10 F11 F12

G G1 G2 G3 G4 G5 G6 G7 GB G9 G10 G11 G12

H

iH1

H2 H3 H4 H5 H6 H7 H8 H9 H10 H11 H12

-15-I

,

l¡

_'

lt

¡¡

_ll

F

_ll

,

_lt

*

_llr-r*rl

rteu

<+

rst

:FI

arl f

eno

rrd

4rh

I Z 4 E FJ ? o ! _{Itl ]I} la ¿ E I'11 PI gI J¿ JJ JD EO E ü D E F L-l H

f,urrent op: f, Itero I,leue: HEsAg

l,.*l

ls.-"-i{@

tiil

IffiI

Figure 22

The procedure of the position:

a)

Click "ltem" to select the item;

b)

Click "S,B,N,P,D,QC" to select the type of the sample;

c)

CIick the hole on the screen to position;

d)

When

multi-items position,

after

finishing

the 1't

item,

click

the

;

"2nd,3'd,4tn"

_to

_{select the other item.}

_CIick

_the

">>"if position

the

Sth

-12th item. After changing the item, the positioned hole will be dark.

-

Sample

User

could click any

hole

to set the

Sample.CIick

the

hole again

to

modify

the Sample No.The area of the Sample No. is from 1 to gg9.

-

Blank

The blank is used to do zero adjustment.lt means that all the other hole's OD

should

minus

the

Blank

OD.lf the

BIank

is

not

positioned,

the default blank

is

zero.

Every item can position more than one blank holes.lf there are several blank holes in the item, the result only displays a mean blank value.

-

Negative Reference

The

negative reference

is valid only in the

Cut-Off items.User

can

position

the

negative

reference

or

positive

reference

according

to

the

Cut-Off

formula.Every item can position more references. lf there are several Ref. holes

in the item, the result only displays a mean Ref. value.

The values

of

the

Ref

are

saved

automaticly.lf

the

next

test

of

the

item

doesn't position the reference, the program will use the saved reference value.

-

Positive Reference

Positive Reference is set as same as Negative Reference.

l.

--

Standard

The Standard is valid only in the quantitative items.The user should position all the standards before reading the plate.lf the item has the saved standards,it is

not necessary to position the standards.

-Quality

Control

Valid if the item has QC.

-

Clear

User can click

the

hole

to

clear after select

the

"Clear" _button.

_And

_{only the}

hole of the current item can be cleared.

-

Stencil

The

Stencil

button is

used

to save the

positioned

_{holes as}

_a

_{stenci!. Click}

"Stencil" _{after finishing}

_the

_position

_to

_go

_{to the}

_{stencil operation interface. Input}

the name of

the

new stencil and click "Add" _{to save the stencil. Click "Modify" or} "Delete" button to modify or delete the selected stencil. Load Stencil function is in

the first interface of the test.

-

Batch

Click "Batch"

to

enter

the

menu

of

batch position. Only

the

sample

can

be

batch positioned. Select

the

Sample

No., Start hole and

the

number

of

the sample to finish the batch position.

The ways to position the hole:

o

Click the hole to set a hole;

o

click

the

number

(1-1»

to set a column of holes;

o

Click the

alphabet

(A-H)

to set a line of holes;

o

Click the "*" _{to set all the holes.}

All the ways is valid to Clear operation.

6.5 Plate

test

After

all the

settings, click "Ok"

to

move

the

plate

and

read

the

absorbance automaticly. The

first

time of the

test

needs some minutes

to

stablizate

the

light source as Figure 23.

I

-t7-The liqht iE sttrhlE Te=t 45ü IJavelength Setting f iIl:er

6.6

Result

view

and

print

After

the

plate reading, click

the

"Position"

to

view

the

position

of the

plate.

CIick

the

"ABS"

to view the value of

absorbance. CIick

the

sample hole to

modify

the value.Attention: lf the value is

Iarger

than

4.000A,the value will

display as 4.00*;lf the value

is

less than

0.000A,the

value will display

as

0.00*.

Click "Qualitative" to view the qualitative of the result according to the setting of the item.

Click

"Quantitative"

_to

view the

calculated

value. In

the

Cut-Off item,

the

quantitative value is the value

of

S/CO. But in the quantitative item, the value is

the calculated concentration.

CIick "standard" to view the standard curve.

The default setting

is to

show

the

result

of

all

the

itenns. If only

one

item is

wanted, just click the Num or >> _to shift the item

.

Click AII to view all the items.

CIick "Save" to save all the values of the plate. The memory of the instrument can save more than two thousand plates.

Click "Print"

to

print

all

the

values

by the

position.

The

holes

that

not positioned will be neglected automaticly as Figure 24.

I

-..*-l

Figure 23

PIEre l,lo. :3 {{ ¡II }} ¿11 Lsr End 3rd 4th

t 4 5 6

¿ U. UU5 U. UUU .5. ¿

B U. UUU U. U9tr u. .)5tf . UJtJ

I U. UI4I U. UUU U. U9ts u. 554 I. UJ!

D U. UIJ U. UUU q. UU tt_ ltqñ . UJ9

E U. UUtr U. U9U T. UqU

F G H $ Position €IAEs $ tluantitatiue Q Qualitative Sar¡e I Print

Page Dounl Standard lFeturn

Figure 24

-18-7

Patient lnformation

Click

the

"Patient lnfo" icon in

the

main menu

to patient information as Figure 25.

enter the setting

of

the

Fatirnt Inf¡ ll¡ 5 l,ln. ll a¡: e

+

I I Feter

|

_'"--

,,,

I

F"'=

,-,;l

Figure 25

7.1 Summariz.ation

Select the Test Date to view the list of the samptes. The default sending date is the current date.

There are two modes of filling the information:

+

First set the patient information:

Click

the'Add'key

to set the _{patient information, and the first column}

_flag

_1,

show that the patient's information is finished already.

+

First test the sample:

After finishing

the

sample test, the

first

column

flag o

show that the tested

sample has

no

patient information. Select

the o

row and click 'Modify,

_to

_input

the patient information.

The meaning of the flag in the first column:

a

Patient information existed, but no sample test result.

o

sample test result existed, but no patient information.

r

Both the patient information and the sample test result existed.

lf the patient information and the sample test result both exist, click ,Modify,

to modify the patient information.

lf

_{the added sample number already exists in the}

_test

_{result, the system will}

automaticly combine the two contents.

Click the Delete to delete the selected _{patient's information and test results.}

7

.2

lnformation

records

The patient information can be added

or

modified in

the

patient info menu or

I--l

|

,,r'.,

I

I

--..=l

I

t-.-rl

Test Ilete

-19-in the patient searching menu.

Sample NO.: Every patient has one and only sample number in one day. The

sample number cannot be changed when modifying;

Name: 18 characters at most;

Sex: Select from Male, Female and unknown; Age: the area of age is from 0

to

150;

Bed No.: 9 characters at most;

Record No.: the record number in hospital, g characters at most;

sample:

the type of sample, defined in the word book;

Department: the sending department, defined in the word book;

Sender'

the sending doctor, defined in the word book; Laboratory: the clinical laboratory, defined in the word book; Analyzer:

the

analyzing doctor, defined in the word book;

Send date: the date sending the sample, defined in the word book; Test date: the date testing the sample, defined in the word book.

Click OK to confirm the input, Esc to cancel the input as Figure 26.

SaupIe Sample N0. llaue 5ex Age Eed l,lo. Fecord Iüo.

l-t"=l

Dep.Etloent

_liEEffiEE

Sender LEborEtorl¡ Inalyzer Sead DEte

t-t

It"b'*t"EI

t--lffirl

2007-03-09 lH"r.

E

f-pl

t-l

t-l

Test date

ffitE

Figure 26

8

System Setting

Click,system'icon

in

the

main menu and

go

into

the

system setting menu.

There are four functions: System set, System log, Data delete and Word Book as

Figure 27.

-20-SIEtenl

Figure 27

8.1

System

setting

Select

the

'System

_set'

_key

_{and go}

_into

_the

_{system setting menu}

_as

_Figure

28:

seriar

r,ro. ( rD

I

reúua:-rlrll

HDsIritEI I,lame FelrüEt Tj.tte

Dare

rir¡e

_I

¿trrr.-o"-r6l

_IEI

Lar:guage _{lE"qli=h E} Fri-nteE _ITIr,==r"I -E

LrlD _{trtrnrrasr EI-}

=lE Iil,:rk Hode _{15r""cl.-"1"",=E}

Hai-rrter-leRce Touch Defar-r-lt oh CanceI

EE

E

ffi

E

EE

Figure 28

1)

Serial

No.

(

_ID)

:

_Show

_the

_identifier

_{of the}

_instrument,

_not

_allowed

modified;

2)

Hospital Name: The name will appear on top of the patient report;

3)

Report Title: The name w¡ll appear following the Hospital Name;

D

Date: Set the date of the system;

5)

Time: Set the time of the system;

6)Language:System supports Chinese and English;

Such

as

chang¡ng the language option,

you

need to press "OK'' button

to

restart

the instrument to take effect;

The Date and Time is used in the test and report, please adjust the date and

the time before us¡ng the instrument.

7

)

Printer: Show the type

of the

printer,

_{the default printer is}

_the

_internal thermal printer. The internal thermal printer has three keys: OPEN key is pressed

to

open

the

door

for

adding

the

thermal paper (Attention:

The

outer side

of

the paper is the thermal layer. The thermal layer must touch the thermal head.); SEL

key is red when online, LF key is green when the power is on. Press SEL to turn

I

-2t-off the red

Iamp and press LF

to

send

the

paper.

Turn

on the

power pressing the SEL, and then loose the key to do the self-test;

8)

LCD

contrast:

Used to adjust the brightness of the LCD back light;

9)

Work mode: Select the stand-alone mode

orthe

PC control mode.

Attention: Before using

the

PC software, please choose

the 'PC

control'

mode. The 'Sample test' key will disappear in

the

PC control mode, and

the program should be in the main menu when using the PC software.

10)

Maintenance: Some maintaining and calibrating functions;

11)

Touch: Used

to

calibrate the touch screen. The touch screen is already

calibrated before leaving factory, so do the calibration only when necessary.

The use of the calibration:

Firstly calibrate

the

abscissa

K:

CIick

the left

part

of the

screen,

the

screen wil! display the real pixel point, then click the right part of the screen, there will be

a

pixel point

also, if the

horizon distance between

the two

points

is

longer than

the two clicks, decrease

the

abscissa

K,

othenryise increase

the

abscissa

K.

Adjust

the K

until

the

distance

is the

same. Secondly calibrate

the

abscissa B:

CIick

anywhere

on the screen,

if

the displayed pixel point

is

not in the

same position of the click, adjust abscissa B until the abscissa of the click is accurately.

The calibration of

the

coordinate is the same as

the

abscissa. After finished the calibration, click OK to save and ESC to quit.

12)

Default: Restore to

the

leaving factory state,

be deleted.

The

initial setting includes

23 test

items

But

the

setup in

the

System Config

will be

remained the touch screen and the setup of the filters.

Click

OK to save the new

setting, otheruvise the after shut down.

Ádjust lhscissa Abscissa

_@l

r. trrl J,hscissa

_@l

ü Idjust ürdinate rJrdinate

_@

I. ü3 Drdinate

_@

-Z

and all

the

user's input will

and

some common words.

including

the

calibration of

new

setting

will

disappear

E

E

E

E

EIear Default 0k Esc

Figure 29

'Maintenance'

_key

_to

go

into the

system

Click

the

_ZZ_

Figure

29. The

_{maintaining module consists}

_{of four}

_functions:

Add new

filters, testing the _{new lamp, verify the repeatability and advanced function.}

o

Filters Setup: The start menu of the maintaining is _{used to add the filters.} The filter wheel can instatl nine filters

at

most. When adding filters,

firs¡y

select the _{number of total filters, then fill in the wavelength}

of the filters in

turn, at last press _{Calibrate Key to save the setting. Attention:}

lf you want

to

_{add filters, please contact with}

_the

_manufacturer

_{or the}

_distributor.

Please don't dismantle the instrument and add uncertain filters.

o

Testing

the

new lamp: Click

the

'Lamp' key

to

go

into

the

new

tamp

testing

_{menu. Firstly select}

_{the one filter and click start}

key, adjust the

position

_{of the lamp by}

_hand,

_{the value on the}

screen

will

change with

the movement of the lamp. When _{the screen is full of data, click} ,,Stop,,

to

stop the test. Click

"Page down"

or

"Page up"

_{to view the}

_{next screen.} Keep adjusting

the

lamp until

the

maximum value.

The

normal value of the baseline is more than 15000.

o

Verify

the

repeatability: Click

the

'Repeat' key

to go

into the repeatability

test

_{menu. Firstly select one filter,}

_test

_times

_and

_row.

_The

range

of

the

test times is

5-8.

Click "Start" key _{to test the certain row for 5 to B times.}

And the

average

value,

repeatability

_{and the}

_CV

_will

_show

_{when all}

_the

tests are finished.

o

Advanced functions:

_{Click the}

'Advanced'

_key

_to

_{go into the}

_advanced functions as Figure 30. This module is only used by the serviceman.

Add ner,r fll_1:er

FirtEr r

_lEr]

Filter

I

_lEl

Fi.rter ¡

_lEl

Fllter 4 Filter 5 Filter tl Filt:er ? Filter E Fil-l:r=r I'¡tE

l-r

t-t

l-t

l.

EI

I

4ü51 Figure 30

8.2

System

Log

\cte

the _{important information of the system.}

I

Ll¡lilrrate

"r' r t ¡r-l1t L al:p Fe¡rs¡¡ Á dr.r 6¡1¡ ¡ 6 J e t¡-¡ r¡

8.3 Date Delete

ln the

system menu

as

Figure 31

_,

click

the

'Data Delete' key

to go

into

the

menu of data deleting. tt is suggested that

the

user delete the old data after

half an year to keep the system faster.

There are five ways of deleting the patients:

o

Delete the patients' data three months ago.

o

Delete the patients' data one month ago.

o

Delete all the _{Patients'data.}

o

And delete the QC data by item and date.

o

Delete the Plate data by date and plate.

DfrtE DE]-ÉIE

tüiPatients QDeIEtE dBtE three üonths Eg'l

fiDelete data one Ioonth aga (-iDelet:e aII patlents dñt8

fr

[[

uata

fl Plate Dat:a

|

,-,.=.-

,-"1

|

**.-"

I

Figure 31

8.4

Word

book

ln the

system

menu as Figure

32,

click the 'Word

book'

to go into

the editing interface of the wordbook.

There

are

seven items

in the

workbook: Laboratory, Department, Analyzer, Sender , Sample, Diagnosis and Unit.

Firstly select the radio circle of the editing item, then edit;

Add: fill in the text box, and click

the'Add'k"y;

Modify: Select the item and fill in the textbox, then click the 'Modify' key;

Delete: Select the item and click the 'Delete' key.

-24-Ii'lrd Enok

Irl c Diagnosis

Add I'l'rdity DeIrte FEturn

Figure 32

9

Search

Click the

'search'

icon in the ma¡n menu

as

Figure 33 to go into the menu of data searching.

The

_{Patient Data, ltem Report, Plate,}

_QC

_{Report, Standard and} Statistics

can be

selected from

the column'search

Mode'.

And

more

detail

condition

_{can be}

_selected

_from

_the'search

_Options'.

_After

_selecting

_the

searching mode and option, just click

'oK'

to begin searching.

Ltk E o¡ fi Lah o r r r:,: rlC, D epartrnerr t Q Inallzer fi Ser:der Q Saup]e (}l _filagrrosis ff Llnlt Figure 33

9.1

Patient

data

searching

Set the option of each searching and check the option box. lf the option box

s '"rot checked, the searching cond¡tion is not valid.

lf

the

patient information

_is

_not

_avaitable,

_the

_sample

_data

_cannot

_be

searched in the patient _report.

It is suggested to set the content of each search¡ng _{option before check the}

::tcn

box and to select the date before the name and sample number.

The table of

the

patient _{data searching result has five columns including the}

fi Petierrt fi Iteu fi Plate fi tltl Feport fi Star:,3ard fi 5tatlst1,-:s

l-l

l-l

l-l

l--l

Send Date TeBt DÉt:E I'leme SanpIe I,Ja.

-25-send¡ng date, sample number, name, sex and the total numbers.

Select the patient in the table. Click

the

'patient report' key

to

see the patient

reports. Click

the

'patient information' key

to

see

the

information

of the

patient.

CIick

the

'Print' key

to

print

the

patient's report. The

instrument supports the

consecutive printing: there is

a

'P' flag in front of the sending date after selecting

the patient. Select all the reports you want to print and just click 'Print' key to print them.

The

sample

with no

patient information

can not be

searched

in the

patient searching.

9.2

ltem searching

The function of

the

module is

to

search

for the

records of

the

same item by

the date as Figure 34.

fi Patient !ffirten fr Flnte S 0c Feport

fi

Standard

fi

Statlstlcs 0k I Esc Figure 34

The

item searching result show

the

patient's name, sample number, result value and the qualitative of the specific item and date as Figure 35.

200?-03-ü9 HBeIg table Sauple totEl:94

Fet. EreE: ünit:

Iüo I,l aro e SaupIe EeEuIt 0uEIitEt]"ue

¡

I j _{8. e25 Positiue} D J 0. 5r4 Iüegatlve 4 4 D. 588 I,¡egÉtlvE 5 5 0. 626 Iüegative 6 6 2. 09e PositiIIe 7 7 0. 663 I'¡EgEtive I D O. 5BÜ Negative

lr"s-

u;l

IF"s-

D*;l I

P.d*l

I

F*,*

I Figure 35

9.3 Plate

Searching

Select Plate mode and set the date and the plate number to search the plate

hrB"

I

l-

,otnrx-ilr]

Iteu I'lBüÉ

TeEt Dü1:e

-26-I

Search Search

I

atrtrr{f-orl

t--tr

TEst DEte FLEi:E No. Figure 36

9.4 QC

Searching

Select

QC report

mode

and set the item

name, month

and the

number to search the QC data as Figure 3T .

as Figure 36. DEtB Search llode ff Patlent ff Iten S PIate fi [tI Fepnrt fi Stenderd fi Strtistlcs 0k Esc Data Search Hode fi Patlent ff Iten fi PIate S tJtI Eepnrt fi 5t:r=ndard Q Stntistics rlk Esc Figure 37

The searching result show the QC

data.. X..

SD and CV

value"

The 2nd

eC

has a pref¡x of *.

click

"QC graph"to view the graph of

_ec

as Figure 3g.

I: Test Date YrIue

¡

2 007-03- 10 I4. 9U rl 2007-03-I1 ¿. ?Srl ?- _?007-03-I1 1s. 961 :3 It07-03-t 2 2.7ü4 -( _{i00?-03-12 I5. 4rl3} 00?-03-13 ¿. toJ ":í:007-03-13 r5. t75 _r. 2007-03-14 4. 995 2 00?-03-14 r. ₁₄₁ Unit:

Eatch II,:. :3tlü?DI

X = Í.50ü 5D = ll.Züll llt = 20.953 Erttrh I,lrr. :3t10?ll4 X = 15.ll0[ 5D = 1.üú[ l]t = 25. Elll XüD?-rl3 T4 QC graph ürl I ürl E X=2. 50[ X=18. 0r]0 5D=ü. SrlD SF=I. rl00 CT=2r1. 953 rtT=ZS. 6üI 15n -t5D -Z5D -35Dü

-, - !! Page Dorun Delete tII liraph _Feturn

Figure 38 on zt -Searrh

n*r*F-Tl

I

emi-oi-os _I

l-l

9.5

Standard Searching

Select Standard mode and set the item name to search the standard data as

Figure 39. Data Seerch Search I'lode fi Patient: fi Iten ff Plate fi tJtJ Feport S Standard fi Statlstics 0k Esc Figure 39

TheCut-Off item shows the absorbance of the reference and the formula.

The quant¡tative item shows the table of the concentration and absorbance of the standards, and the graph of the standards as Figure 40.

HEs,$g Standtrrd

Hepatltis E Surface Intigen rlut-0f f Erl ¡ ü. 2ü6

FEF AE5 FoErouIE Ilalue T¡] ü. 098 e. l+I'¡1 0. zü6 llTUt Standard 'l1:n I UuLti rlon. ÁE5 f . il[t] tl . üEs 2. 0Etl rl . 480 3. ü0ú r_. 1¿¿ 4. 800 r. 450 5. 080 I q'Jq s.0

9.6

Statistics

F;l@

Search I,lode Q Petient Q lteu S PIate S Q[ Feport fi Standard [üiStetistics Figure 40 Setup =nt

l-.,".1

ffi

SarupIe rlc $earch Setup

t"lffiñTl

*

_{l-iñ'?-n-'ffill}

[k I E=c Figure 41

The

Statistics Function

is

used in the statistics

of

the QC data in the custom¡zing time as Figure 41 &42.

]¿l 1. É 0. B tt-tt-_{--8.0 1.8 3.6} 5.4 Q Patien fiIteu 5 S It:ero 0 StErt DE1 End Date

FEtlent: StEtlEtlcE Fstient nufi: I

Ido Ilonth I,¡ilI,1

I ¿tltl ? - 01 rl Stltl?-tlE rl Stltl? -tl5 I

l-r"-*',rl

I

r"r-

,-,*l

I

--,*"

I

Figure 42

10

Report

In

the

main menu

as

Figure 43, click

the

'Report' key

to go

into

the

patient

report menu. The patient report is

a

shortcut of

the

latest patient searching. The

result

of

searching

by the

patient

in the

latest sending

date is as

same

as

the patient report list.

Patlent TahIe

IF"-1=*

F-r-*l tp".1*.

rr,f"l

Test: Üate SaropIe I,J ar¡ e 5e>r T'lteI a

¡

z L-rtlT -tl3 - rl ! Z I ? ¿007-t:t3-r:rl J I eüü?-rt3-0s 4 3ütl?-tl3-tls tr I ¡rlrl?-tl3-tltl E 1 30t_t? - tl3 -tl g _{T 1} ¿rlr:tT-[3-t]S _I StltlT-tl3-üs 3

Ptge l-l¡ Fage Doun Select AII Frir:t Feturn

Figure 43

The module

is

used

to

search and print

the

latest patient report quickly. The

patient

table

is

in the latest

sending

day. If there

is

no

patient

in the

day, the result may be in the old days.

Select the printing line, and the first column of the line will appear a "P" which

means

the

line

will be

printed.

Multilines

can be

selected

to

print. And

click "Select AIl" to select all the lines in the table. If there are more than one P before

the

line,

the

patient report

will be

printed

out

continuously.

The

P

will

disappear after report was printed.

Click

the

"Patient

_Report"

_or

_{"Patient lnfo" key}

_to

_view

_the

_report

_or

_the

information of the selected patient as Figure44.

-29-ljEIÍFl-E Un. :.1- I,laue: f'EtEr :iex:Ila.Le nr_re: Iü

f t: e¡: F.rsr-rIl: Llrlit:./[r-¡a1 I,lsrüsl- Are¡

HEeA,-t 1. [1¡] Fosltl'.re

Faqe I.J¡ Fage DErrrn &dd ur=l-tr LE Prlut F e tr-r rn

Figure 44

11

Shut

Down

When finished

us¡ng

the

instrument,

shut down the

power directly.

All

the saved data is stored in the FIash memory and reliable for long time.

12 PC

mode

12.1

Software

lnstal

lation

The

instrument

can

be controlled

by

a

PC

through

the

serial

port,

us¡ng

the

PCsoftware

interface,

more

powerful,

easy to

connect hospital management system,if using this method, the instrument must be set

as

follows: (software, see the software online help)

o

Click

"System

rr

o

Select the "PC Mode"

.

CIick "OK"

Note: To

install

the

software, please

contact

the dealer or manufacturer. Set

to"PC Control", the main interface on the "sample test" button will disappear.

12.2

Software Operation

Operating

systeffr:

Windows XP

Hardware

environment

¡

Pentium ll1350

above,

memory 128M, 500M or more free hard disk capac¡ty, resolution 800 * 600 and above

Communication:

Seria! Communication

Communication lnterface:

RS232 serial port

Gommunication baud rate

:19200

12.3

Software ser¡al

communication protocol

and code

1) Frame

format

Table 4

Frame head Sequence length PDU type Data Check

A5H 1 byte 2 bytes 2 bytes N bytes 1 byte

t

-30-Frame

head:

Because synHeader is ASH, therefore _{may not appear the same}

byte as ASH in the other fields in a frame, in order to avoid the frame analysis

grammatical ambiguity;

if

in

other

fields

appears

_{AsH, then in after that}

_fills

again an ASH byte. Receiver when

analysis,

meets single

AsH

byte to think it

is the synHeader,

meets

two

consecutive

AsH

byte

to think

it

is

other data

byte,

this time should neglect an ASH byte.

Sequence:

Back end may wilfully

fill

in; front end must

fill

in

the

same

sequence number rn the response frame.

Length:

The

length

is the total

length

of

PDU

type field and

data

field,

not including the synHeader, sequence and checkcode

PDU:

See

Part2

Data:

See Part 2

Check:

Single-byte XOR _{checksum algorithm, computation scope: sequence}

number, length, PDU type, and data.

2)

PDU

type and

data

0x0001

reset

0x0002

Read the

front

version

0x0003

front version

0x00'10

command completes and no data is returned

0x0011

command to complete, _{but an error occurred}

0x0101

shake; Vibration ELISA ptate

0x0102

switch the tight fitter

0x0103

read the

air's

AD value

0x0104

AD value

0x0105

ELISA test board

0x0106

ABS Vatue

0x0107

AD value

of

the last test

0x0108

AD value

0x0109

lamp

switch (0

Open,

1 Close)

0x010A

read lamp's state

0x010B

lamp's state

0x010C

read machine's lD

0x010D

machine's lD

-3)

Send

commands and responses

Table 5

Type Command function

Receive /Send

Data

Expected response

Timeout Other explanations

0x0001 reset Send 0x001 0 5 seconds

After power on,use this

command

to

judge

whether the correction of coefficient is correct 0x0002 Read the front vers¡on Send none 0x0003 1 seconds

0x0003 front version Receive 4 bytes none none

0x001 0

Command comPletes and no data is returned

recelve none none none

0x0011

Command

to

complete, but

an

error occurred

recetve two bytes none none

0x0101 Vibration ELISA Plate Send

Two

bYtes

first

bYte

ls

speed Second byte

is

times 0x0010 Vibration time +3 seconds Vibration SPeed :

1.

slowly

2.

middle

3.

fast Vibration time: 1 -60 seconds

0x0102 switch

the

light filter Send

one byte:

Filter number(0-7)

0x0010 3 seconds

installs seven light filters, the user can

increase two

0x0103

read

the

air's

AD value

Send none 0x0104 1 second

0x0104 the air's AD value Receive

16

bytes

of

AD value

none none

I

row

16 AD value of the air

0x01 05 ELISA

test

board Send

One byte test mode

(0: continuous; 1: Step) 0x0106 Continuous: 1 Oseconds Step: 30 seconds

&

-32-0x01 06 Absorbance test Value Receive 192 bytes, 96 holes

of

the

16 absorbance value none 1 seconds

192 bytes of data, all

values have expanded

1,000 times

10005

absorbance said too

much 10,006 that is too small absorbance

0x0107 AD value

of

the last test Send none 0x01 08 1 seconds

Executive Directive to be tested

0x0108 AD value Receive

208 bytes, 96 holes

of the 16 AD value

+8 rows the 16 air AD

none 1 seconds

0x0109 lamp switch send

0

means open, means closed

0x0010 I second

0x010A read lamp's state send none 0x01 0B 1 second

0x01 0B lamp's state recerve

one

byte: lamp's state

two bytes: switch lamp time

none none

0x01 0C read machine's ID send none 0x010D 1 second

0x010D machine's lD recetve

nine

bytes: machine's lD none none I

-33-4)

Error number

0x1101 self-test error

\

0x1102 Order overtime 0x1103 lllegal orders

0x1 1 04 lllegal orders investigation

0x1201 lnvalid filter

Ox1202lnvalid Vibration ELISA plate time

Oxl2OS lnvalid Vibration ELISA plate speed Ox1204lnvalid Vibration ELISA plate mode

0x1205 No reduction filter signal

0x1206 ELISA plate without reset signal

Ox12O7 ELISA plate fixed or positioning signal slot Optocoupler

0x1208 AD not OK signal

0x1 209 lnvalid voltages

0x120F Light source is too strong 0x1210 Light source is too weak

5)

Serial communication parameters

Baudrate: 19200

Parity: 'N'

Databits:8

Stopsbits:1

-34-IS09001&

IS013485

Certif

ied

Sinnowa

Medical

Science & Technology

Co.

,

Ltd

Acld

0ilin

Industri¿rl

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ing Chin¿r

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