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(1)

Photosynthesis

Photosynthesis

(2)
(3)

Autotrophs

Autotrophs

Plants and some

Plants and some

other types of

other types of

organisms that

organisms that

contain

contain

chlorophyll

chlorophyll are are

able to use

able to use light light energy from the energy from the

sun

sun to produce to produce food.

(4)

Autotrophs

Autotrophs

Autotrophs

Autotrophs

include

include

organisms

organisms

that

that

make their own

make their own

food

food

Autotrophs can

Autotrophs can

use the sun’s

use the sun’s

energy directly

(5)

Heterotrophs

Heterotrophs

Heterotrophs Heterotrophs are organisms are organisms

that can NOT that can NOT

make their own make their own

food food

(6)

Energy

Energy

Energy Takes Many Energy Takes Many

Forms such as

Forms such as light, light, heat, electrical,

heat, electrical,

chemical, mechanical chemical, mechanical

Energy can be changed Energy can be changed from

from one form to one form to another

another

Energy can be stored in Energy can be stored in chemical bonds & then

chemical bonds & then

released later

(7)

ATP – Cellular Energy

ATP – Cellular Energy

Adenosine TriphosphateAdenosine Triphosphate

Contains two, high-energy phosphate Contains two, high-energy phosphate bonds

bonds

Also contains the nitrogen base adenine Also contains the nitrogen base adenine & a ribose sugar

(8)

ADP

ADP

Adenosine DiphosphateAdenosine Diphosphate

ATP releases ATP releases energyenergy, a , a free free phosphate,

phosphate, & & ADPADP when cells take when cells take

energy from ATP

(9)

Sugar in ADP & ATP

Sugar in ADP & ATP

Called ribose

Pentose

sugar

(10)

Importance of ATP

Importance of ATP

Principal

Compound

Used To

Store

Energy

In

Living

(11)

Releasing Energy From ATP

Releasing Energy From ATP

ATP is constantly being ATP is constantly being used and used and

remade by cells remade by cells

ATP provides all of the energy for ATP provides all of the energy for cell activities

cell activities

The high energy phosphate bonds The high energy phosphate bonds can be

can be BROKENBROKEN to to release energyrelease energy

The process of releasing ATP’s The process of releasing ATP’s

energy & reforming the molecule

(12)

Releasing Energy From ATP

Releasing Energy From ATP

AddingAdding A A

Phosphate Group

Phosphate Group

To ADP stores

To ADP stores

Energy in ATP

Energy in ATP

RemovingRemoving A A

Phosphate Group Phosphate Group From ATP From ATP Releases Energy Releases Energy

& forms ADP

& forms ADP

Lose

(13)

Cells Using Biochemical Energy

Cells Using Biochemical Energy

Cells Use ATP For:

Active transportMovement

Photosynthesis

Protein SynthesisCellular respirationAll other cellular

(14)

More on ATP

More on ATP

Cells Have Enough ATP To

Cells Have Enough ATP To

Last For A

Last For A

Few Seconds

Few Seconds

ATP must

ATP must

constantly

constantly

be

be

made

made

ATP

ATP

Transfers Energy

Transfers Energy

Very Well

Very Well

ATP Is

ATP Is

NOT

NOT

Good At

Good At

Energy Storage

(15)

Glucose

Glucose

Glucose is a

Glucose is a

monosaccharide

monosaccharide

C

C

66

H

H

1212

O

O

66

One Molecule of glucose

One Molecule of glucose

Stores

Stores

90 Times

90 Times

More

More

Chemical Energy Than

Chemical Energy Than

One Molecule of ATP

(16)

History of

History of

Photosynthesis &

Photosynthesis &

(17)

Photosynthesis

Photosynthesis

Involves the Use Of light Involves the Use Of light

Energy to convert

Energy to convert Water (HWater (H220)0) and

and Carbon Dioxide (COCarbon Dioxide (CO22)) into into

Oxygen (O

Oxygen (O22)) and and High Energy High Energy

Carbohydrates

Carbohydrates (sugars, e.g. (sugars, e.g.

Glucose) & Starches

(18)

Investigating Photosynthesis

Investigating Photosynthesis

Many Scientists Have

Many Scientists Have

Contributed To

Contributed To

Understanding Photosynthesis

Understanding Photosynthesis

Early Research

Early Research

Focused On

Focused On

The

The

Overall Process

Overall Process

Later Researchers

Later Researchers

Investigated The Detailed

Investigated The Detailed

Chemical Pathways

(19)

Early Questions on Plants

Early Questions on Plants

Several Centuries Ago, The

Several Centuries Ago, The

Question Was:

Question Was:

Does the increase in mass

Does the increase in mass

(20)

The Photosynthesis

The Photosynthesis

Equation

(21)

Pigments

Pigments

In addition to water, carbon dioxide, and light energy,

photosynthesis requires

Pigments

Chlorophyll is the

primary light-absorbing pigment in autotrophs

(22)

Light and Pigments

Light and Pigments

Energy From The Sun

Enters Earth’s Biosphere As Photons

Photon = Light Energy

Unit

Light Contains A Mixture Of Wavelengths

Different Wavelengths

(23)

Light & Pigments

Light & Pigments

Different pigmentsDifferent pigments absorb absorb

different

different wavelengthswavelengths of light of light

Photons of light Photons of light “excite”“excite” electrons electrons in the plant’s pigments

in the plant’s pigments

Excited electrons carry the Excited electrons carry the absorbed energy

absorbed energy

Excited electrons move to Excited electrons move to

HIGHER

(24)

Chlorophyll

Chlorophyll

There are 2 main types

There are 2 main types

of chlorophyll

of chlorophyll

molecules:

molecules:

Chlorophyll a

Chlorophyll a

Chlorophyll b

Chlorophyll b

A third type,

A third type, chlorophyll chlorophyll c

c, is found in , is found in dinoflagellates

(25)

Chlorophyll a and b

(26)

Chlorophyll a

Chlorophyll a

Found in all

Found in all

plants, algae, &

plants, algae, &

cyanobacteria

cyanobacteria

Makes photosynthesis possible

Makes photosynthesis possible

Participates

Participates

directly

directly

in the

in the

Light Reactions

Light Reactions

Can

Can

accept energy

accept energy

from

from

chlorophyll b

(27)

Chlorophyll b

Chlorophyll b

Chlorophyll b is an

Chlorophyll b is an

accessory pigment

accessory pigment

Chlorophyll b acts

Chlorophyll b acts

indirectly

indirectly

in photosynthesis by

in photosynthesis by

transferring the light it

transferring the light it

absorbs to chlorophyll

absorbs to chlorophyll

a

a

Like chlorophyll a, it

Like chlorophyll a, it

absorbs red & blue light

absorbs red & blue light

and

(28)

The Biochemical

The Biochemical

(29)

It Begins with Sunlight!

(30)

Photoautotrophs Absorb Light

Photoautotrophs Absorb Light

Energy

(31)

Inside A Chloroplast

(32)

Structure of the Chloroplast

Structure of the Chloroplast

Double membrane

Double membrane

organelle

organelle

Outer membrane

Outer membrane

smooth

smooth

Inner membrane

Inner membrane

forms stacks

forms stacks

of connected sacs called

of connected sacs called

thylakoids

thylakoids

Thylakoid stack is called the

Thylakoid stack is called the

granun

granun

(grana-plural)

(grana-plural)

Gel-like material around grana

Gel-like material around grana

called

(33)

Function of the Stroma

Function of the Stroma

Light Independent

Light Independent

reactions occur here

reactions occur here

ATP used

ATP used

to make

to make

carbohydrates like

carbohydrates like

glucose

glucose

Location of the

Location of the

Calvin

Calvin

Cycle

(34)
(35)

Thylakoid membranes

Thylakoid membranes

Light DependentLight Dependent reactions occur reactions occur here

here

Photosystems are made up of Photosystems are made up of clusters of chlorophyll molecules

clusters of chlorophyll moleculesPhotosystems are Photosystems are embedded in embedded in

the thylakoid membranes

the thylakoid membranes

The two photosystems are:The two photosystems are:

Photosytem IPhotosytem I

(36)

Photosynthesis Overview

(37)

Energy Carriers

Energy Carriers

Nicotinamide Adenine Dinucleotide Nicotinamide Adenine Dinucleotide

Phosphate

Phosphate (NADP(NADP++))

NADPH

NADPH

= =

Reduced

Reduced

Form

Form

Picks Up

Picks Up

2 high-energy

2 high-energy

electrons and H

electrons and H

++

from the Light

from the Light

Reaction to form NADPH

Reaction to form NADPH

NADPH

NADPH

carries energy to be

carries energy to be

passed on to another molecule

(38)

NADPH

(39)

Occurs across the

Occurs across the

thylakoid

thylakoid

membranes

membranes

Uses light energy

Uses light energy

Produce

Produce

Oxygen

Oxygen

from water

from water

Convert

Convert

ADP to ATP

ADP to ATP

Also convert

Also convert

NADP

NADP

++

into the

into the

energy carrier

energy carrier

NADPH

NADPH

Light Dependent Reactions

(40)

Light Dependent Reaction

(41)

Light Dependent Reaction

(42)

Photosystem I

Photosystem I

Discovered

Discovered

First

First

Active in the final stage of

Active in the final stage of

the Light Dependent Reaction

the Light Dependent Reaction

Made of 300 molecules of

Made of 300 molecules of

Chlorophyll

Chlorophyll

Almost completely chlorophyll

Almost completely chlorophyll

a

(43)

Photosystem II

Photosystem II

Discovered

Discovered

Second

Second

Active in the beginning stage

Active in the beginning stage

Of the Light Dependent

Of the Light Dependent

Reaction

Reaction

Contains about

Contains about

equal amounts

equal amounts

of chlorophyll a and

of chlorophyll a and

chlorophyll b

(44)

Photosynthesis Begins

Photosynthesis Begins

Photosystem

Photosystem

II absorbs light energy

II absorbs light energy

Electrons are energized

Electrons are energized

and passed

and passed

to the

to the

Electron Transport Chain

Electron Transport Chain

Lost electrons are replaced from

Lost electrons are replaced from

the splitting of water

the splitting of water

into 2H

into 2H

+, +,

free electrons, and Oxygen

free electrons, and Oxygen

2H

2H

+ +

pumped across thylakoid

pumped across thylakoid

membrane

(45)

Photosystem I

Photosystem I

High-energy electrons

High-energy electrons are are

moved to Photosystem I

moved to Photosystem I

through the Electron

through the Electron

Transport Chain

Transport Chain

Energy is used to

Energy is used to transport Htransport H++

from stroma to inner

from stroma to inner

thylakoid membrane

thylakoid membrane

NADP+ converted to NADPH NADP+ converted to NADPH

when it picks up 2 electrons &

when it picks up 2 electrons &

H+

(46)

Phosphorylation

Phosphorylation

Enzyme in thylakoid

Enzyme in thylakoid

membrane called

membrane called

ATP

ATP

Synthase

Synthase

As H+ ions passed through

As H+ ions passed through

thylakoid membrane,

thylakoid membrane,

enzyme binds them to ADP

enzyme binds them to ADP

Forms

(47)
(48)

Light Reaction Summary

Light Reaction Summary

Reactants:

Reactants:

H

H

22

O

O

Light Energy

Light Energy

Energy Products:

Energy Products:

ATP

ATP

(49)

Light Independent Reaction

Light Independent Reaction

ATP & NADPH

ATP & NADPH

from light

from light

reactions used as energy

reactions used as energy

Atmospheric

Atmospheric

C0

C0

22

is used

is used

to

to

make sugars

make sugars

like glucose and

like glucose and

fructose

fructose

Six-carbon Sugars made

Six-carbon Sugars made

during the

during the

Calvin Cycle

Calvin Cycle

(50)

The Calvin Cycle

(51)

The Calvin Cycle

The Calvin Cycle

Two turnsTwo turns of the Calvin Cycle of the Calvin Cycle are required to make one

are required to make one

molecule of glucose

molecule of glucose

3-CO3-CO22 molecules enter the cycle molecules enter the cycle to form several

to form several intermediate intermediate compounds (PGA)

compounds (PGA)

A 3-carbon molecule called A 3-carbon molecule called Ribulose Biphosphate

Ribulose Biphosphate (RuBP) is (RuBP) is used to regenerate the Calvin

used to regenerate the Calvin

cycle

(52)
(53)

Factors Affecting the Rate

Factors Affecting the Rate

of Photosynthesis

of Photosynthesis

Amount of

Amount of

available water

available water

Temperature

Temperature

Amount of

Amount of

available light

available light

energy

(54)

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