Photosynthesis
Photosynthesis
Autotrophs
Autotrophs
Plants and some
Plants and some
other types of
other types of
organisms that
organisms that
contain
contain
chlorophyll
chlorophyll are are
able to use
able to use light light energy from the energy from the
sun
sun to produce to produce food.
Autotrophs
Autotrophs
•
Autotrophs
Autotrophs
include
include
organisms
organisms
that
that
make their own
make their own
food
food
•
Autotrophs can
Autotrophs can
use the sun’s
use the sun’s
energy directly
Heterotrophs
Heterotrophs
• Heterotrophs Heterotrophs are organisms are organisms
that can NOT that can NOT
make their own make their own
food food
Energy
Energy
• Energy Takes Many Energy Takes ManyForms such as
Forms such as light, light, heat, electrical,
heat, electrical,
chemical, mechanical chemical, mechanical
• Energy can be changed Energy can be changed from
from one form to one form to another
another
• Energy can be stored in Energy can be stored in chemical bonds & then
chemical bonds & then
released later
ATP – Cellular Energy
ATP – Cellular Energy
• Adenosine TriphosphateAdenosine Triphosphate
• Contains two, high-energy phosphate Contains two, high-energy phosphate bonds
bonds
• Also contains the nitrogen base adenine Also contains the nitrogen base adenine & a ribose sugar
ADP
ADP
• Adenosine DiphosphateAdenosine Diphosphate
• ATP releases ATP releases energyenergy, a , a free free phosphate,
phosphate, & & ADPADP when cells take when cells take
energy from ATP
Sugar in ADP & ATP
Sugar in ADP & ATP
•
Called ribose
•
Pentose
sugar
Importance of ATP
Importance of ATP
Principal
Compound
Used To
Store
Energy
In
Living
Releasing Energy From ATP
Releasing Energy From ATP
• ATP is constantly being ATP is constantly being used and used andremade by cells remade by cells
• ATP provides all of the energy for ATP provides all of the energy for cell activities
cell activities
• The high energy phosphate bonds The high energy phosphate bonds can be
can be BROKENBROKEN to to release energyrelease energy
• The process of releasing ATP’s The process of releasing ATP’s
energy & reforming the molecule
Releasing Energy From ATP
Releasing Energy From ATP
• AddingAdding A A
Phosphate Group
Phosphate Group
To ADP stores
To ADP stores
Energy in ATP
Energy in ATP
• RemovingRemoving A A
Phosphate Group Phosphate Group From ATP From ATP Releases Energy Releases Energy
& forms ADP
& forms ADP
Lose
Cells Using Biochemical Energy
Cells Using Biochemical Energy
Cells Use ATP For:
• Active transport • Movement
• Photosynthesis
• Protein Synthesis • Cellular respiration • All other cellular
More on ATP
More on ATP
•
Cells Have Enough ATP To
Cells Have Enough ATP To
Last For A
Last For A
Few Seconds
Few Seconds
•
ATP must
ATP must
constantly
constantly
be
be
made
made
•
ATP
ATP
Transfers Energy
Transfers Energy
Very Well
Very Well
•
ATP Is
ATP Is
NOT
NOT
Good At
Good At
Energy Storage
Glucose
Glucose
•
Glucose is a
Glucose is a
monosaccharide
monosaccharide
•
C
C
66H
H
1212O
O
66•
One Molecule of glucose
One Molecule of glucose
Stores
Stores
90 Times
90 Times
More
More
Chemical Energy Than
Chemical Energy Than
One Molecule of ATP
History of
History of
Photosynthesis &
Photosynthesis &
Photosynthesis
Photosynthesis
• Involves the Use Of light Involves the Use Of lightEnergy to convert
Energy to convert Water (HWater (H220)0) and
and Carbon Dioxide (COCarbon Dioxide (CO22)) into into
Oxygen (O
Oxygen (O22)) and and High Energy High Energy
Carbohydrates
Carbohydrates (sugars, e.g. (sugars, e.g.
Glucose) & Starches
Investigating Photosynthesis
Investigating Photosynthesis
•
Many Scientists Have
Many Scientists Have
Contributed To
Contributed To
Understanding Photosynthesis
Understanding Photosynthesis
•
Early Research
Early Research
Focused On
Focused On
The
The
Overall Process
Overall Process
•
Later Researchers
Later Researchers
Investigated The Detailed
Investigated The Detailed
Chemical Pathways
Early Questions on Plants
Early Questions on Plants
Several Centuries Ago, The
Several Centuries Ago, The
Question Was:
Question Was:
Does the increase in mass
Does the increase in mass
The Photosynthesis
The Photosynthesis
Equation
Pigments
Pigments
• In addition to water, carbon dioxide, and light energy,
photosynthesis requires
Pigments
• Chlorophyll is the
primary light-absorbing pigment in autotrophs
Light and Pigments
Light and Pigments
• Energy From The Sun
Enters Earth’s Biosphere As Photons
•
Photon = Light Energy
Unit
• Light Contains A Mixture Of Wavelengths
•
Different Wavelengths
Light & Pigments
Light & Pigments
• Different pigmentsDifferent pigments absorb absorb
different
different wavelengthswavelengths of light of light
• Photons of light Photons of light “excite”“excite” electrons electrons in the plant’s pigments
in the plant’s pigments
• Excited electrons carry the Excited electrons carry the absorbed energy
absorbed energy
• Excited electrons move to Excited electrons move to
HIGHER
Chlorophyll
Chlorophyll
There are 2 main types
There are 2 main types
of chlorophyll
of chlorophyll
molecules:
molecules:
Chlorophyll a
Chlorophyll a
Chlorophyll b
Chlorophyll b
A third type,A third type, chlorophyll chlorophyll c
c, is found in , is found in dinoflagellates
Chlorophyll a and b
Chlorophyll a
Chlorophyll a
•
Found in all
Found in all
plants, algae, &
plants, algae, &
cyanobacteria
cyanobacteria
•
Makes photosynthesis possible
Makes photosynthesis possible
•
Participates
Participates
directly
directly
in the
in the
Light Reactions
Light Reactions
•
Can
Can
accept energy
accept energy
from
from
chlorophyll b
Chlorophyll b
Chlorophyll b
•
Chlorophyll b is an
Chlorophyll b is an
accessory pigment
accessory pigment
•
Chlorophyll b acts
Chlorophyll b acts
indirectly
indirectly
in photosynthesis by
in photosynthesis by
transferring the light it
transferring the light it
absorbs to chlorophyll
absorbs to chlorophyll
a
a
•
Like chlorophyll a, it
Like chlorophyll a, it
absorbs red & blue light
absorbs red & blue light
and
The Biochemical
The Biochemical
It Begins with Sunlight!
Photoautotrophs Absorb Light
Photoautotrophs Absorb Light
Energy
Inside A Chloroplast
Structure of the Chloroplast
Structure of the Chloroplast
•
Double membrane
Double membrane
organelle
organelle
•
Outer membrane
Outer membrane
smooth
smooth
•
Inner membrane
Inner membrane
forms stacks
forms stacks
of connected sacs called
of connected sacs called
thylakoids
thylakoids
•
Thylakoid stack is called the
Thylakoid stack is called the
granun
granun
(grana-plural)
(grana-plural)
•
Gel-like material around grana
Gel-like material around grana
called
Function of the Stroma
Function of the Stroma
•
Light Independent
Light Independent
reactions occur here
reactions occur here
•
ATP used
ATP used
to make
to make
carbohydrates like
carbohydrates like
glucose
glucose
•
Location of the
Location of the
Calvin
Calvin
Cycle
Thylakoid membranes
Thylakoid membranes
• Light DependentLight Dependent reactions occur reactions occur here
here
• Photosystems are made up of Photosystems are made up of clusters of chlorophyll molecules
clusters of chlorophyll molecules • Photosystems are Photosystems are embedded in embedded in
the thylakoid membranes
the thylakoid membranes
• The two photosystems are:The two photosystems are:
Photosytem IPhotosytem I
Photosynthesis Overview
Energy Carriers
Energy Carriers
• Nicotinamide Adenine Dinucleotide Nicotinamide Adenine Dinucleotide
Phosphate
Phosphate (NADP(NADP++))
•
NADPH
NADPH
= =Reduced
Reduced
Form
Form
•
Picks Up
Picks Up
2 high-energy
2 high-energy
electrons and H
electrons and H
++from the Light
from the Light
Reaction to form NADPH
Reaction to form NADPH
•
NADPH
NADPH
carries energy to be
carries energy to be
passed on to another molecule
NADPH
•
Occurs across the
Occurs across the
thylakoid
thylakoid
membranes
membranes
•
Uses light energy
Uses light energy
•
Produce
Produce
Oxygen
Oxygen
from water
from water
•
Convert
Convert
ADP to ATP
ADP to ATP
•
Also convert
Also convert
NADP
NADP
++into the
into the
energy carrier
energy carrier
NADPH
NADPH
Light Dependent Reactions
Light Dependent Reaction
Light Dependent Reaction
Photosystem I
Photosystem I
•
Discovered
Discovered
First
First
•
Active in the final stage of
Active in the final stage of
the Light Dependent Reaction
the Light Dependent Reaction
•
Made of 300 molecules of
Made of 300 molecules of
Chlorophyll
Chlorophyll
•
Almost completely chlorophyll
Almost completely chlorophyll
a
Photosystem II
Photosystem II
•
Discovered
Discovered
Second
Second
•
Active in the beginning stage
Active in the beginning stage
Of the Light Dependent
Of the Light Dependent
Reaction
Reaction
•
Contains about
Contains about
equal amounts
equal amounts
of chlorophyll a and
of chlorophyll a and
chlorophyll b
Photosynthesis Begins
Photosynthesis Begins
Photosystem
Photosystem
II absorbs light energy
II absorbs light energy
Electrons are energized
Electrons are energized
and passed
and passed
to the
to the
Electron Transport Chain
Electron Transport Chain
Lost electrons are replaced from
Lost electrons are replaced from
the splitting of water
the splitting of water
into 2H
into 2H
+, +,free electrons, and Oxygen
free electrons, and Oxygen
2H
2H
+ +pumped across thylakoid
pumped across thylakoid
membrane
Photosystem I
Photosystem I
High-energy electrons
High-energy electrons are are
moved to Photosystem I
moved to Photosystem I
through the Electron
through the Electron
Transport Chain
Transport Chain
Energy is used to
Energy is used to transport Htransport H++
from stroma to inner
from stroma to inner
thylakoid membrane
thylakoid membrane
NADP+ converted to NADPH NADP+ converted to NADPH
when it picks up 2 electrons &
when it picks up 2 electrons &
H+
Phosphorylation
Phosphorylation
Enzyme in thylakoid
Enzyme in thylakoid
membrane called
membrane called
ATP
ATP
Synthase
Synthase
As H+ ions passed through
As H+ ions passed through
thylakoid membrane,
thylakoid membrane,
enzyme binds them to ADP
enzyme binds them to ADP
Forms
Light Reaction Summary
Light Reaction Summary
Reactants:
Reactants:
•
H
H
22O
O
•
Light Energy
Light Energy
Energy Products:
Energy Products:
•
ATP
ATP
Light Independent Reaction
Light Independent Reaction
•
ATP & NADPH
ATP & NADPH
from light
from light
reactions used as energy
reactions used as energy
•
Atmospheric
Atmospheric
C0
C0
22is used
is used
to
to
make sugars
make sugars
like glucose and
like glucose and
fructose
fructose
•
Six-carbon Sugars made
Six-carbon Sugars made
during the
during the
Calvin Cycle
Calvin Cycle
The Calvin Cycle
The Calvin Cycle
The Calvin Cycle
• Two turnsTwo turns of the Calvin Cycle of the Calvin Cycle are required to make one
are required to make one
molecule of glucose
molecule of glucose
• 3-CO3-CO22 molecules enter the cycle molecules enter the cycle to form several
to form several intermediate intermediate compounds (PGA)
compounds (PGA)
• A 3-carbon molecule called A 3-carbon molecule called Ribulose Biphosphate
Ribulose Biphosphate (RuBP) is (RuBP) is used to regenerate the Calvin
used to regenerate the Calvin
cycle
Factors Affecting the Rate
Factors Affecting the Rate
of Photosynthesis
of Photosynthesis
•
Amount of
Amount of
available water
available water
•
Temperature
Temperature
•
Amount of
Amount of
available light
available light
energy