11β-Hydroxysteroid dehydrogenase 1
Characterisation of 11β-hydroxysteroid dehydrogenase 1 in human orbital adipose tissue: a comparison with subcutaneous and omental fat
... in 1 ! Hanks’ Balanced Salt Solution (Gibco Invitrogen Corporation) for 45 min at 37 ...for 1 min, and the supernatant containing connective tissue debris, collagenase and lipid was removed leaving a pellet ...
11
11β Hydroxysteroid dehydrogenase – 1 activity in type 2 diabetes mellitus: a comparative study
... in 11β HSD 1 activity with diabetes ...increased 11β HSD1 activity in diabetics < 35 years of ...examining 11β HSD1 with DHEAS, the matter cannot be ...
9
Evidence for transcript-specific epigenetic regulation of glucocorticoid-stimulated skeletal muscle 11β-hydroxysteroid dehydrogenase-1 activity in type 2 diabetes
... Figure 1 outlines the location of the HSD11B1 promoter methylation assays used in this ...promoter 1 (P1); and 11HSDB1_F2, 5 0 -aggaagagagTTTTTGAA AGATTATTGATTTTTGG and 11HSDB1_R2, 5 0 -cagt ...
8
Increased Expression of 11β Hydroxysteroid Dehydrogenase Type 1 in Experimental Periodontitis Induced by Lipopolysaccharide from Porphyromonas gingivalis
... glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (#Rn01- 7757-63_g1) were purchased from Applied Biosystems (Foster City, CA, ...RNA-to-CT 1-Step Kit and Step One Plus Real Time PCR System (Applied ...
10
11β Hydroxysteroid Dehydrogenase Type 1 Inhibition Attenuates the Adverse Effects of Glucocorticoids on Dermal Papilla Cells
... of 11β-HSD1 and its regulation by cortisol in ...of 11β-HSD1 can partially prevent glu- cocorticoid-induced suppression of proliferation, growth fac- tor expression, and the expression of dermal papilla ...
7
11β Hydroxysteroid dehydrogenase blockade prevents age induced skin structure and function defects
... for 1 hour in TBSAT (10 ml 10× TBS ...diluted 1:100 before washing under agitation in three 10-minute washes of TBST (~800 ml 1× TBS plus ...diluted 1:200 before repeating ...
11
Differential expression, function and response to inflammatory stimuli of 11β-hydroxysteroid dehydrogenase type 1 in human fibroblasts: a mechanism for tissue-specific regulation of inflammation
... enzyme 11β-HSD1, which exhibited cell-specific and cytokine-specific variations in expression and ...cell 11β- HSD1 has previously been reported in adipocytes [32], oste- oblasts [33] and amnion-derived ...
11
Inhibition of 11β–hydroxysteroid dehydrogenase type II selectively blocks the tumor COX 2 pathway and suppresses colon carcinogenesis in mice and humans
... on the C57BL/6 background were obtained from The Jackson Laboratory. The mice were fed a high-fat diet (0.2% cholesterol, 21% saturated fat, for- mula TD88173; Harlan Teklad) from 6 wk of age and treated with vehicle ...
11
11β hydroxysteroid dehydrogenase glucocorticoid metabolism within the lung and its influence on macrophage function in the acute respiratory distress syndrome
... The receptor for advanced glycation end products (RAGE) concentrations in plasma and BALF were measured using a commercially available ELISA kit (R&D systems, Minneapolis, Minnesota, USA). This kit contains a ...
261
Differential expression, function and response to inflammatory stimuli of 11β hydroxysteroid dehydrogenase type 1 in human fibroblasts: a mechanism for tissue specific regulation of inflammation
... enzyme 11β-HSD1, which exhibited cell-specific and cytokine-specific variations in expression and ...cell 11β- HSD1 has previously been reported in adipocytes [32], oste- oblasts [33] and amnion-derived ...
10
11β-Hydroxysteroid dehydrogenase type 1 within muscle protects against the adverse effects of local inflammation
... selective 11β-HSD1 inhibitor LJ2 (1000 nmol/l), or a combination of the ...selective 11β-HSD1 inhibitor LJ2 (1000 nmol/l) or a combination of both cortisone and inhibitor for 16 ...
12
Dietary fatty acid composition alters 11β-hydroxysteroid dehydrogenase type 1 gene expression in rat retroperitoneal white adipose tissue
... 11b-hydroxysteroid dehydrogenase type 1 (11b-HSD1) plays an important role in the development of obesity and insulin resistance. 11b-HSD1 reactivates inactive glucocor- ticoids ...
5
Reduced activity of 11β hydroxysteroid dehydrogenase in patients with cholestasis
... ditionally, this abnormality is attributed to secondary hyperaldosteronism as a consequence of renal hypop- erfusion related to intra-abdominal fluid sequestration (13). This concept has been questioned due to the ...
8
The expression of 11β-hydroxysteroid dehydrogenase type 1 is increased in experimental periodontitis in rats
... and 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) inactivates ...of 11β-HSD1 was increased in adipo- cytes, especially in visceral fat, of patients with metabolic diseases, which ...
7
Epigenetic regulation of 11β hydroxysteroid dehydrogenase type 2 expression
... part 1 (nucleotides –704 to –380) and part 2 (nucleotides –403 to –76) were considered (Figure ...parts 1 and 2), an effect that correlated with higher expression of the HSD11B2 gene (Figure ...parts ...
13
Regulation of 11β Hydroxysteroid Dehydrogenase Type 1 and 2 in Rheumatoid Arthritis
... Over the 24 weeks period disease activity of RA de- creased significantly (median PV week 0 = 1.85 mPa (IQR 1.76 - 1.90), week 12 = 1.77 (IQR 1.71 - 1.89), week 24 = 1.77 (1.66 - 1.84), p < 0.01; median AI week 0 = 24 ...
9
Uncovering steroidopathy in women with autism: a latent class analysis
... encodes 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD-1) ...in 11β-HSD-1 cause androgenic precursors to accu- mulate in the adrenal gland, resulting in ...
12
QSAR Studies on Keto Sulfones Derivatives as Inhibitors of 11β Hydroxysteroid Dehydrogenase Type 1
... intercorrelation matrix of the descriptors of QSAR Equations, (1) to (3) is given in Table 3. Descriptors having intercorrelation above |r|>0.5 were not considered while deriving the QSAR model. The predictor ...
9
Renal 11β-hydroxysteroid dehydrogenase activities: Pharmacological inhibition and putative regulators
... between 1 and 13nmol/l, and for cortisol, 14-60nmol/l (Naray-Fejes-Toth et ai, 1991; Brown et ai, 1993; Kenouch et ai, 1994; Stewart et ai, 1994; Yang and Yu, ...
255
Regulation of lipogenesis by glucocorticoids and insulin in human adipose tissue
... 11b-Hydroxysteroid dehydrogenase type 1 (11b-HSD1) and we have hypothesized that 11b-HSD1 activity can regulate lipid homeostasis in human adipose tissue (Chub-S7 cell line and primary cultures of ...
10