152LR(+)/152LR(–) RNA
Lehmann, Elisabeth (2011): Structure-function analysis of RNA polymerase II: use of RNA as a template and site-directed mutagenesis in S. cerevisiae. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... synthesise RNA in an RNA-templated manner, but the activity is much slower and less processive than the DNA-dependent ...HDV RNA replication only from the genomic but not from the antigenomic strand ...
111
RIG I ATPase Activity and Discrimination of Self RNA versus Non Self RNA
... before RNA transfection, short duplex molecules such as 9r-11d, 10r-10d, 11r-9d, and 12r-8d, which are inactive in unprimed cells, became active ...the RNA rec- ognized as a PAMP ...
12
Editorial focus: entering into the non-coding RNA era
... The correlation between genotype and phenotype has become the Holy Grail of bio- medical research in recent decades. High-throughput genotyping technologies such as deep sequencing have dramatically enhanced our ...
7
Vaccinia virus RNA polymerase associated with nuclei of infected HeLa cells.
... RNA polymerase I and vaccinia RNA polymerase were not distinguished in the intact nuclei, but hybridization of 3% of the RNA synthesized by nuclei from infected cells in the presence of [r] ...
9
Complementary RNA Species Isolated from Vesicular Stomatitis (HR Strain) Defective Virions
... Presence or absence of plus and minus strand RNA in VSV virion populationsa RNA sample VSV HR defective virion RNA Ribonuclease resistance of 3H-RNA % None or melted Self annealed None o[r] ...
11
Comparison of LCx with Other Current Viral Load Assays for Detecting and Quantifying Human Immunodeficiency Virus Type 1 RNA in Patients Infected with the Circulating Recombinant Form A/G (CRF02)
... before being subjected to statistical analysis. Values were com- pared by nonparametric tests, as indicated. Data were also analyzed by plotting the difference between measurements ob- tained by two methods against their ...
5
Bovine Papilloma Virus Transcription: Polyadenylated RNA Species and Assessment of the Direction of Transcription
... The with ClaI; b, RNA from normal bovine skin; c, RNA majority of the sequences of the 2-kb RNA are from peripheral wart tissue; d, RNA from basal wart confined to an area of the genome [r] ...
8
RNA associated with murine intracisternal type A particles codes for the main particle protein.
... Slot 1 No added RNA; slot 2 total polyadenylated RNA from N4 A-particles; slot 3 32S RNA from N4 A-particles; slot 4 35S RNA from N4 Aparticles; slot 5 28S RNA from MOPC-104E A-particles[r] ...
9
Action spectra for UV- light induced RNA-RNA crosslinking in 16S ribosomal RNA in the ribosome.
... In this work the action absorption spectrum for eight out of fifteen 16S rRNA crosslinks was determined. It was possible to analyze these molecules because they had electrophoretic mobilities in the gel electrophoresis ...
40
Identification of a Natural Viral RNA Motif That Optimizes Sensing of Viral RNA by RIG I
... double-stranded RNA, have been identified as essential for the triggering of antiviral ...Most RNA viruses expose these motifs during their replication; however, successful viruses normally evade immune ...
11
RNA-Based Immunity Terminates Viral Infection in Adult Drosophila in the Absence of Viral Suppression of RNA Interference: Characterization of Viral Small Interfering RNA Populations in Wild-Type and Mutant Flies
... Previous attempts to detect viral siRNAs in FHV-infected WT flies by Northern blot hybridization were unsuccessful (72). Use of a chemical cross-linking protocol (54) in this study allowed reproducible detection of the ...
11
A Cucumber Mosaic Virus (CMV) RNA 1 Transgene Mediates Suppression of the Homologous Viral RNA 1 Constitutively and Prevents CMV Entry into the Phloem
... CMV RNA 1 is characterized by reduced virus accumulation in the inoculated leaf, with specific suppression of accumulation of the homol- ogous viral RNA 1, and by the absence of systemic ...viral RNA ...
7
Influenza B virus has global ordered RNA structure in (+) and (−) strands but relatively less stable predicted RNA folding free energy than allowed by the encoded protein sequence
... in the regulation of splicing. When many sequences are available, predicted folding stabilities can identify RNA regions likely to have structure [17]. A survey of all in- fluenza A coding sequences found evidence ...
7
The Murine Norovirus Core Subgenomic RNA Promoter Consists of a Stable Stem-Loop That Can Direct Accurate Initiation of RNA Synthesis
... stem-loop RNA sequence that we predicted to be the MNV sgRNA ...of RNA synthesis by the MNV RdRp in ...GII.4 RNA proscript, GII.4Ps, used for the in vitro RNA synthesis ...subgenomic ...
12
Coronavirus Multiplication Strategy I. Identification and Characterization of Virus-Specified RNA
... Marker labeled poliovirus RNA 0 13 and a nucleic acid extract containing Semliki Forest virus standard virus 42S RNA V and 26S RNA 0, together with Semliki Forest virus DI RNA species DI[r] ...
10
<p>Construction and Analysis of a Long Non-Coding RNA-Associated Competing Endogenous RNA Network Identified Potential Prognostic Biomarkers in Luminal Breast Cancer</p>
... LncRNA, a type of RNA with limited protein-coding ability, is previously viewed as transcriptional “ noise ” without biological functions. 14 Recently, lncRNAs are increasingly reported to be closely involved in ...
12
Structural and Functional Characterization of Sapovirus RNA-Dependent RNA Polymerase
... Generation of synthetic RNA templates. Sapovirus clone pJG-Sap01 (GenBank accession number AY694184), which was characterized by phylogenetic analysis based on the complete sequence of its capsid gene as belonging ...
14
A Polyamide Inhibits Replication of Vesicular Stomatitis Virus by Targeting RNA in the Nucleocapsid
... Thermal shift. assay. Prior to carrying out the thermal shift assay (TSA), the isolation of recombi- nantly expressed NLP was purified according to a protocol previously established by our lab (47). All NLPs were checked ...
15
RNA Interference: An Evolutionary Pathway in Periodontal Tissue Engineering
... antisense RNA to shut down expression of the par-1 gene in order to assess its ...antisense RNA disrupted expression of par-1, but quizzically, injection of the sense-strand control did too used antisense ...
5
Initiation of Genomic Plus-Strand RNA Synthesis from DNA and RNA Templates by a Viral RNA-Dependent RNA Polymerase
... of RNA synthesis, we tested endscripts of the minus strand of cucumber mosaic virus (CMV) RNA2 and RNA3, ...discrete RNA products by the BMV RdRp ...plus-strand RNA synthesis is ini- tiated from the ...
9