16S rRNA gene sequencing
A comprehensive evaluation of the sl1p pipeline for 16S rRNA gene sequencing analysis
... for 16S rRNA gene data processing makes it difficult to identify the most accurate ...library 16S rRNA gene sequencing pipeline (sl1p, pronounced “slip”), a 16S ...
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Quantification of variation and the impact of biomass in targeted 16S rRNA gene sequencing studies
... 469 sequencing libraries across 19 different MiSeq runs (Table ...in sequencing low-biomass samples, given the stochastic variation in- herent during PCR amplification of low quantities of DNA, a set of ...
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Catheter Related Microbacterium Bacteremia Identified by 16S rRNA Gene Sequencing
... the gene sequences of bacterial species has shown that the 16S rRNA gene is highly conserved within a species and among species of the same ...the 16S rRNA gene sequence ...
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Sequence Diversity of Neisseria meningitidis 16S rRNA Genes and Use of 16S rRNA Gene Sequencing as a Molecular Subtyping Tool
... among 16S rRNA genes of 264 N. meningitidis strains, suggesting that 16S rRNA gene sequencing might be a useful molecular typing tool for epidemiological ...certain 16S ...
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Controlling for Contaminants in Low-Biomass 16S rRNA Gene Sequencing Experiments
... after 16S rRNA gene se- quencing have been ...after 16S rRNA library preparation (9, 13, ...from 16S rRNA gene sequencing studies, there is no current ...
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Species Specific Identification of Leptospiraceae by 16S rRNA Gene Sequencing
... leptospires. 16S rRNA gene sequences are potentially useful for species identification of Leptospira, but there are a large number of sequences of various lengths and quality in the public ...
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Application of 16S rRNA Gene Sequencing To IdentifyBordetella hinzii as the Causative Agent of Fatal Septicemia
... 16S rRNA gene sequencing and phylogenetic ...The rRNA gene was amplified by PCR with universal primers 8FPL and DG74 (positions 8 to 27 and 1522 to 1540, respectively; ...
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Use of 16S rRNA Gene Sequencing for Rapid Confirmatory Identification of Brucella Isolates
... evaluate 16S rRNA gene sequencing to deter- mine its utility for confirmatory identification of Brucella ...in 16S rRNA gene sequences may be useful for subtyping or for ...
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Identification of bacteria on the surface of clinically infected and non infected prosthetic hip joints removed during revision arthroplasties by 16S rRNA gene sequencing and by microbiological culture
... used 16S rRNA gene sequencing to identify bacteria in a relatively small number of clinical specimens, adopted the approach of sequencing about 50 clones ...
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Species Specific Identification of Campylobacters by Partial 16S rRNA Gene Sequencing
... of 16S rDNA yield sufficient information to discrimi- nate among the ...taxa. 16S rDNA alignment studies revealed four variable gene regions, which were termed Vc6, Vc5, Vc2, and Vc1, in accordance ...
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Comparison of MALDI TOF MS, Housekeeping Gene Sequencing, and 16S rRNA Gene Sequencing for Identification of Aeromonas Clinical Isolates
... In this study, we compared two housekeeping gene se- quences (gyrB, rpoB), as two or more housekeeping genes sequencing could be useful to improve the reliability of the phylogenies. The results matched in ...
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Three Cases of Anaerobiospirillum succiniciproducens Bacteremia Confirmed by 16S rRNA Gene Sequencing
... FIG. 3. Inferred phylogeny of the proteobacteria, obtained by using complete 16S rRNA gene sequences, showing the relationship of A. succiniciproducens to its closest relatives. Note that Haemophilus ...
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Metagenomic Approach for Identification of the Pathogens Associated with Diarrhea in Stool Specimens
... Pathogen detection by the diagnostic laboratory and research labo- ratory. In the clinical diagnostic laboratory, a glutamate dehydrogenase enzyme immunoassay (EIA) (Wampole C. diff Quik Chek; Alere, Or- lando, FL) was ...
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Usefulness of the MicroSeq 500 16S Ribosomal DNA Based Bacterial Identification System for Identification of Clinically Significant Bacterial Isolates with Ambiguous Biochemical Profiles
... 16S rRNA gene sequencing will continue to be the working gold standard for the identification of most bacteria, and better automation of such a technique may put it into routine use in large ...
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Rapid Detection of Emerging Pathogens and Loss of Microbial Diversity Associated with Severe Lung Disease in Cystic Fibrosis
... It has been recognized that in addition to routine culture-based methodologies, CF microbiology needs to embrace a range of mo- lecular techniques capable of dealing with the complexity of the lung microbiome, in ...
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Streptococcus sinensis sp nov , a Novel Species Isolated from a Patient with Infective Endocarditis
... starch. 16S rRNA gene sequencing showed that there were ...the 16S rRNA gene sequence of the bacterium and those of Streptococcus gordonii, Streptococcus intermedius, ...
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Effect of the specific carbohydrate diet on the microbiome of a Primary Sclerosing Cholangitis and Ulcerative Colitis patient
... sclerosing cholangitis (PSC) at age 16. The PSC was successfully treated with high doses of oral vancomycin; however, the UC was more difficult to manage. After many drug treatments failed to treat the UC, the patient ...
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Study of Bacterial Diversity of Mangroves Rhizosphere
... The morphological and biochemical studies of the 35 isolates were performed according to (see Table 1). Mo- lecular characterization was done using 16S rRNA gene sequencing. Nineteen (19) out ...
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16S rRNA gene-based profiling of the human infant gut microbiota is strongly influenced by sample processing and PCR primer choice
... following 16S rRNA gene sequencing; Additional file 1: Figure S2), possibly due to degradation of DNA released from lysed cells during extended ...
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Genome sequence of a native-feather degrading extremely thermophilic Eubacterium, Fervidobacterium islandicum AW-1
... and 16S rRNA gene sequencing analyses demonstrated that this native chicken feather degrading bacterium belonging to the genus Fervidobacterium was identified as Fervidobacterium islandicum ...
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