2,3-Dioxygenase
Original Article Expression and function analysis of indoleamine 2 and 3-dioxygenase in bladder urothelial carcinoma
... Abstract: Indoleamine 2, 3-dioxygenase (IDO) is a rate-limiting enzyme for tryptophan metabolism inducing immune tolerance of tumors. The purpose of this study is to investigate IDO expression and ...
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H2S suppresses indoleamine 2, 3-dioxygenase 1 and exhibits immunotherapeutic efficacy in murine hepatocellular carcinoma
... Indoleamine 2,3-dioxygenase 1 (IDO1), a ubiquitous mono- meric heme-containing enzyme, is the first rate-limiting en- zyme in the kynurenine pathway (KP) that catabolizes tryptophan (Trp) into kynurenine [1]. ...
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Green Tea Epigallocatechin-3-Gallate Suppresses Autoimmune Arthritis Through Indoleamine-2,3-Dioxygenase Expressing Dendritic Cells and the Nuclear Factor, Erythroid 2-Like 2 Antioxidant Pathway
... (EDTA), pH 7.2, and single cell suspensions were prepared and flow cytometric analysis was performed using a FACSCalibur (BD Biosciences, San Jose, CA) with BD Cell- Quest Pro Software (BD Biosciences) and the data was ...
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The role of indoleamine 2, 3 dioxygenase in regulating host immunity to leishmania infection
... Infectious pathogens provoke tissue inflammation, which causes some host cells (such as macrophages and NK cells) to release IFNs that induce IDO in some cells [41]. Previously, it was assumed that IDO induced by ...
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B7 costimulation and intracellular indoleamine 2,3 dioxygenase (IDO) expression in peripheral blood of healthy pregnant and non pregnant women
... Besides initiating signal transduction in T lymphocytes, B7-1 and B7-2 may back-signal into the APC and influ- ence the local immune environment through induced ex- pression of immunosuppressive factors ...
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Decreased protein nitration in macrophages that overexpress indoleamine 2, 3-dioxygenase
... RAW 264.7 macrophages express nitric oxide synthase [63], which generates nitric oxide that may subsequently react with superoxide anions to form peroxynitrite, which can nitrate proteins on cysteine, methionine, ...
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Indoleamine 2, 3-dioxygenase (IDO) increases during renal fibrogenesis and its inhibition potentiates TGF-β 1-induced epithelial to mesenchymal transition
... synthesis was performed using M-MLV Reverse Tran- scriptase from 1 μl of total RNA according to the manufac- turer’ s protocol (Promega, Madison, USA). Analysis of mRNA expression by reverse transcription RT-PCR was ...
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Original Article Overexpressed indoleamine 2, 3-dioxygenase induced T cell apoptosis via Fas/FasL and Bcl-2/Bax-related apoptotic pathways in metastatic breast cancer
... pIRES2-EGFP vector using RIPA buffer (PBS containing 1% nonidet P-40, 0.5% sodium deoxycholate acid, 0.1% SDS, PMSF (100 ng/ ml), and aprotinin (66 ng/ml)). Proteins were electrophoresed by 12% SDS-PAGE and trans- ferred ...
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Upregulated expression of indoleamine 2, 3-dioxygenase in CHO cells induces apoptosis of competent T cells and increases proportion of Treg cells
... constructed (Figure 1A). Analysis of IDO expression by PCR using genomic DNA, or by RT-PCR using total RNA, yielded a 188 bp fragment; meanwhile, no IDO expression was detected in CHO/EGFP cells, indicating that we could ...
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Indoleamine 2, 3-dioxygenase Up-regulates Hypoxia-inducible Factor-1α Expression by Degrading L-tryptophan but Not Its Activity in Human Alloreactive T-cells
... The primary antibodies used in western blotting were specific for HIF-1α(Cell Signaling Technology; cat. no. 3716; dilution 1:500), p53 (Cell Signaling Technology; cat. no. 9282; dilution 1:500), c-Myc (Cell Signaling ...
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Mesenchymal stem cells overexpressing IL-35: a novel immunosuppressive strategy and therapeutic target for inducing transplant tolerance
... In 2002, Bartholomew et al. [36] observed that MSCs could suppress lymphocyte proliferation in vitro and in vivo. Administration of MSCs led to prolonged skin graft survival in baboons when compared to control animals ...
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Obesity and colorectal cancer: molecular features of adipose tissue
... On the other hand, when tumour is initiated it may arrest inflammation to escape of immune cells. One mechanism to assess this is by decreasing the inflamma- tion signal of adipose tissue through the regulation of ...
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Combination regimens with PD-1/PD-L1 immune checkpoint inhibitors for gastrointestinal malignancies
... gene 3; MANAs: Mutation- associated neoantigens; McAbs: Monoclonal antibodies; MDM2/4: Murine double minute 2/4; MDSCs: Myeloid-derived suppressor cells; MEK: MAP kinse-ERK kinase; MHC: Major ...
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Decision Making Amplification Under Uncertainty: An Exploratory Study of Behavioral Similarity and Intelligent Decision Support Systems
... Quercetin 2,3-dioxygenase (QDO) has been isolated from Aspergillus flavus (CuQDO) and Ba- cillus subtilis (FeQDO) with coordination of the copper and iron, respectively 44 . QDO has two cupin domains thus it ...
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Emerging therapeutic roles for NAD+ metabolism in mitochondrial and age-related disorders
... glyceraldehyde 3-phosphate dehydro- genase; CR: calorie restriction; AMPK: adenosine monophosphate-activated protein kinase; PGC-1α: peroxisome proliferator-activated receptor gamma coactivator-1alpha; FOXO1: ...
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PubMedCentral-PMC4965332.pdf
... Figure 3. Stimulation of fetal human cortical astrocytes with interleukin-1 β (IL-1 β ) and interferon- γ (IFN- γ ). Fetal cultured human cortical astrocytes stimulated with recombinant human IL-1 β (10 ng ml −1 ) ...
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Enhanced therapeutic effect of an antiangiogenesis peptide on lung cancer in vivo combined with salmonella VNP20009 carrying a Sox2 shRNA construct
... C-caspase 3, cleaved Caspase 3; Cfu, colony forming unit; CSCs, cancer stem cells; H&E, hematoxylin and eosin; IDO1, indoleamine 2, 3-dioxygenase 1; IHC, immunohistochemistry; L, ...
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Effects of pro-inflammatory cytokines on expression of kynurenine pathway enzymes in human dermal fibroblasts
... Modulation of transcript-levels by IFN-g and/or TNF-a The potential effects of IFN-g, TNF-a, or a combination of IFN-g and TNF-a on kynurenine pathway transcripts were investigated in the fibroblast cell cultures, see ...
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Pathway engineering of the toluene degradation pathway of Pseudomonas putida mt-2
... 2,3 dioxygenase (C230). C230 catalyses the ring cleavage of catechol, 3-methylcatechol and 4-methylcatechol, and also has weak activity towards ...carbon 2 of the substrate (Cerdan et al, ...
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Endogenous Lipogenic Regulators of Spore Balance in Aspergillus nidulans
... Regulatory links between ppoA and ppoC: mechanism for an oxylipin signature. Champe’s physiological and biochemical characterization of psi factor in the 1980s was among the first studies to uncover a mechanism that ...
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