20-kDa
HAEMATOLOGICAL EFFECTS AND SAFETY BEHAVIOUR OF 20 kDa PEGINTERFERON ALPHA-2A (UNIPEG®) IN HEALTHY HUMAN SUBJECTS
... The 20 kDa pegylated interferon alpha-2a (PEG(20kDa)-IFN-alpha-2a) is a covalent conjugate of the protein interferon alpha-2a (IFN-alpha-2a); produced by recombinant DNA technology in Escherichia coli, with ...
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Interferon-stimulated gene of 20 kDa protein (ISG20) degrades RNA of hepatitis B virus to impede the replication of HBV in vitro and in vivo
... receiving the control plasmid; (○), mice receiving the ISG20 plasmid. (C) The serum HBsAg titers were determined with an enzyme immunoassay at O.D 450 nm [calculated as signal over noise ratios (S/N)]. HBV DNA in the ...
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Expression of TTV ORF2 Protein for Detection of Anti TTV IgG Antibodies in Human Sera
... ORF-2 region of TTV genotype-1 was amplified and cloned in expression plasmid containing histidine tag. It expressed the protein at 8, 12, 16 and 20 hour post incu- bation at 37˚C (Figure 2). Optimal expression of ...
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Reisinger, Veronika (2008): Biogenese photosynthetischer Elektronentransport-Komplexe in Plastiden der Gerste(Hordeum vulgare L.). Dissertation, LMU München: Fakultät für Biologie
... Nach zweistündiger Belichtung ist photochemisch der Elektronenübertrag auf Ferredoxin nachweisbar. Da für die Anlagerung von Ferredoxin an das Reaktionszentrum die Untereinheiten PsaC und PsaD nötig zu sein scheinen ...
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Internal translation of the connexin 43 transcript
... Firstly, we generated a Cx43 frameshift (FS) mutation that prevents the generation of full-length Cx43. For this approach, we took advantage of a naturally existing EcoNI site located only 30 base pairs after the full- ...
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Microfluidic mass production of stabilized and stealthy liquid metal nanoparticles
... of 20 kDa) solution with a concentration of 500 µM, we examined this hypothesis by conducting experiments using microchannels with the same W and H of 5 and ...
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High Molecular Sericin from <i>Bombyx mori</i> Cocoons: Extraction and Recovering by Ultrafiltration - Volume 5 Number 3 (Jun. 2014) - IJCEA
... 15-30 kDa in silk degumming wastewater, where 95 % of rejection was reported for sericin volume concentrataion factor of ...(180 KDa - 200 kDa) with polyethersulfone membrane of 20 kDa, ...
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Epstein-Barr Virus-Encoded RK-BARF0 Protein Expression
... To confirm that the 30- and 35-kDa proteins detected with the rabbit antiserum were of cellular origin, a panel of EBV- negative B-cell lines was examined by immunoblotting by using this antiserum (Fig. 3B). The ...
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Regulation of Closterovirus Gene Expression Examined by Insertion of a Self-Processing Reporter and by Northern Hybridization
... A reporter open reading frame (ORF) coding for a fusion of bacterial  -glucuronidase (GUS) with a proteinase domain (Pro) derived from tobacco etch potyvirus was utilized for tagging individual genes of beet yellows ...
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Ostreolysin A/Pleurotolysin B and Equinatoxins: Structure, Function and Pathophysiological Effects of These Pore-Forming Proteins
... basic 20 kDa equinatoxins (EqTs) are cytolytic proteins isolated from the edible mushroom Pleurotus ostreatus and the sea anemone Actinia equine, ...
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Ostreolysin A/Pleurotolysin B and Equinatoxins: Structure, Function and Pathophysiological Effects of These Pore-Forming Proteins
... around 20 kDa) and, due to their size, it is unlikely that they could pass the brain-blood barrier unless endothelial damage gives access to the neurons of the respiratory centre in the medulla ...
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Antibody Response of Patients withHelicobacter pylori-Related Gastric Adenocarcinoma: Significance of Anti-CagA Antibodies
... the 20-kDa ferritin-like protein (Felp), the 26- and 35-kDa antigens, the 54-kDa catalase, the 60-kDa HspB, the 76-kDa fumarate reductase, the 87-kDa VacA, and the 125 ...
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Expression of PE38 and IE2, viral members of the C3HC4 finger family, during baculovirus infection: PE38 and IE2 localize to distinct nuclear regions.
... 38 kDa was detected with the PE38 antiserum (Fig. 5). The lack of the 20-kDa polypep- tide confirms that viral factors are necessary for its ...
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Human Cytomegalovirus Stimulates Monocyte-to-Macrophage Differentiation via the Temporal Regulation of Caspase 3
... reported to block cytochrome c release from the mitochondria, which in turn results in the inhibition of the initial cleavage of pro-caspase 3 (45). Because protein expression of both HSP27 (Fig. 4B) and Mcl-1 (7) is ...
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Article - Genetic relationship among Citrus Species on the basis of protein profiling obtained by comparative electrophoresis
... 6-50 kDa. Four bands in the range of 35 kDa, 20kDa, 10 kDa and 4kDa were conserved in all the ...35 kDa and 20 kDa bands with consistent appearance showed similarity with the ...
5
Gleaning evolutionary insights from the genome sequence of a probiotic yeast Saccharomyces boulardii
... 54 kDa serine protease from Sb was reported to provide protection against ...120 kDa protein has been suggested to play a role in neutralizing the secretion induced by the cholera toxin by reducing cyclic ...
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Molecular Weight Determination of a Protease Extracted from Mucor pusillus: Comparison Methods
... Separation was achieved in decreasing order of molecular weight on the gel Sephadex G-100 with the help of a column (1 × 60 cm) Pharmacia calibrated by a phosphate buffer solution (0.02 M; pH 6). Aliquot 1.5 mL sample ...
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Regulation of platelet-activating factor-mediated interleukin-6 promoter activation by the 48 kDa but not the 45 kDa isoform of protein tyrosine phosphatase non-receptor type 2
... 48 kDa, generated by alternative splicing in human and rat cells, but only the 45 kDa isoform is found in murine cells [26, ...48 kDa is found in the Jurkat T cell line and the THP-1 myeloid cell ...
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Regulation of the biosynthesis of subgroup C adenovirus protein IVa2.
... Protein IVa2 A to E and the 43-kDa protein F were visualized with IgGs affinity purified against the 54-kDa viral and 43-kDa cellular proteins, respectively, as described in Materials an[r] ...
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Expression of the 60 kDa and 71 kDa heat shock proteins and presence of antibodies against the 71 kDa heat shock protein in pediatric patients with immune thrombocytopenic purpura
... Heat shock proteins (Hsp) are highly conserved proteins found in prokaryotes and eukaryotes. Induction of Hsps can be triggered by many stresses. These include exposure to supraoptimal temperatures (heat shock) and to ...
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