2D-DIGE
Combination of laser microdissection, 2D-DIGE and MALDI-TOF MS to identify protein biomarkers to predict colorectal cancer spread
... by 2D-DIGE with saturation CyDye labeling and identified using MALDI-TOF mass spectrom- ...microdissection, 2D DIGE with saturation labelling and MALDI-TOF MS proved to be resourceful ...
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2D DIGE analysis of maternal plasma for potential biomarkers of Down Syndrome
... to 2D DIGE over the pH ...representative 2D gel image with the differentially expressed proteins between the two groups highlighted, whilst Figure 2 gives a 3D representation of the ceruloplasmin ...
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2D-DIGE proteomic analysis identifies new potential therapeutic targets for adrenocortical carcinoma
... a 2D-DIGE approach applied for the first time to the screening of proteomic expression in ACC, we identified an overexpression protein profile associated with ...
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Original Article Vimentin and PRG4 as candidate biomarkers of ovarian cancer using serum proteomic analysis of 2D-DIGE
... Abstract: Epithelial ovarian cancer (OC) is the most lethal of gynecologic malignancies, largely due to the advanced stage at diagnosis in most patients. It is therefore an urgent task to screen out reliable biomarkers ...
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2D-DIGE proteome analysis on the platelet proteins of patients with major depression
... after 2D-DIGE, the gels were scanned with a Typhoon Trio imager using an excitation/emission filter of 488/520 nm for Cy2, 532/580 nm for Cy3, and 633/ 670 nm for Cy5 to generate multiplexed DIGE ...
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Differential analysis of glioblastoma multiforme proteome by a 2D-DIGE approach
... Our 2D-DIGE analysis confirmed a higher expression in GBM samples for GRP-75 and also two additional chaperone proteins: the reticulum endoplasmic protein disulfite iso- merase (ER-60, ERp57 or GRP-57) and ...
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Identification of potential protein biomarkers for early detection of pregnancy in cow urine using 2D DIGE and label free quantitation
... the DIGE gel in the present investigation is shown in ...all DIGE gels are shown in Additional file 1: Figure ...the DIGE gel in Decyder soft- ware, we observed a total of 11 differentially expressed ...
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First evidence of overlaps between HIV-Associated Dementia (HAD) and non-viral neurodegenerative diseases: proteomic analysis of the frontal cortex from HIV+ patients with and without dementia
... for 2D-DIGE, with additional confirmation by immunohistochemitsry (IHC) using frontal lobe tissue from different HAD and HIV+ non-dementia ...the DIGE results, lending further credence to the current ...
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Differential proteome analysis in adolescent idiopathic scoliosis patients with thoracolumbar/lumbar curvatures
... in 2D-DIGE analysis were excised from the Sypro®Ruby-s- tained gels by a robotic picker (Spot-picker®, GE Health- care) and digested using 2 μL trypsin and 50 mM ammonium hydrogen carbonate ...
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Identification of cellular proteome using two-dimensional difference gel electrophoresis in ST cells infected with transmissible gastroenteritis coronavirus
... (2D DIGE) is reproducible and sensitive [13]. 2D DIGE has greatly facilitated the comparison of two samples by removing gel-to-gel variability and by using dyes (CyDye) with a greater dynamic ...
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Biomarkers of inflammation and innate immunity in atrophic nonunion fracture
... Bone formation is controlled by many factors acting locally to regulate proliferation, differentiation and activ- ity of osteoblasts. TGF-β and BMPs, as well as ligands of Wnt family are of course very well known ...
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Secretome analysis of rice suspension-cultured cells infected by Xanthomonas oryzae pv.oryza (Xoo)
... Background: Rice bacterial blight (BB) caused by Xanthomonas oryzae pv .oryzae ( Xoo) is one of the most devastating bacterial diseases in rice-growing regions worldwide. The rice-Xoo interaction is a classical model for ...
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Original Article Identification of phosphorylated MYL12B as a potential plasma biomarker for septic acute kidney injury using a quantitative proteomic approach
... Abstract: Acute kidney injury (AKI) is a common and increasingly encountered complication in hospitalized patients with critical illness in intensive care units (ICU). According to the etiology, Sepsis-induced AKI (SAKI) ...
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PPAR Signaling Pathway and Cancer-Related Proteins Are Involved in Celiac Disease-Associated Tissue Damage
... Celiac disease (CD) is an immune-mediated disorder triggered by the ingestion of wheat gliadin and related proteins in ge- netically predisposed individuals. To find a proteomic CD diagnostic signature and to gain a ...
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A proteomic view of Caenorhabditis elegans caused by short-term hypoxic stress
... by 2D gel electrophoresis liquid chromatography (2D-LC) combined with electro- spray ionization tandem mass spectrometry (ESI-MS/ MS) and identified a total of 1,616 proteins, including 110 ...
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Proteomic analysis of the cerebrospinal fluid of patients with restless legs syndrome/Willis-Ekbom disease
... CSF was utilized for this biomarker study because it is most likely to reflect changes in CNS metabolic status due to its proximity to the brain. An advantage of CSF as the biological fluid for proteomic analyses over ...
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Desmin expression in colorectal cancer stroma correlates with advanced stage disease and marks angiogenic microvessels
... Proteomic determination of factors expressed by tumor cells and host stromal cells, either inherently or as a result of tumor-host interactions, has been shown to be useful in elucidating molecular pathways of tumor ...
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The molecular pathogenesis of feline calicivirus infection
... of 2D-DIGE as a means to compare proteomes from different infection states gives a good first impression of the differences in protein expression between the cellular ...technique. 2D-DIGE ...
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A unique protein profile of peripheral neutrophils from COPD patients does not reflect cytokine-induced protein profiles of neutrophils in vitro
... non-smokers in the PCA showed clustering of COPD spotmaps apart from control spotmaps (data not shown). Based on these observations we find it unlikely that smok- ing is the reason for the differential neutrophil protein ...
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Synovial membrane protein expression differs between juvenile idiopathic arthritis subtypes in early disease
... 2D DIGE: Two dimensional fluorescent difference in-gel electrophoresis; 2D PAGE: Two dimensional polyacrylamide gel electrophoresis; ANOVA: Analysis of variance analysis; CHAPS: ...
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