3-Butanediol
Simultaneous production of isopropanol, butanol, ethanol and 2,3-butanediol by Clostridium acetobutylicum ATCC 824 engineered strains
... butanone) (Jones and Woods 1986; Xiao and Xu 2007), very low concentrations of acetoin were detected in cul- tures of transformants expressing the adh gene. How- ever, D and/or L 2,3-butanediol (2,3-BD) ...
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Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2,3-butanediol production
... glycerol from 2,3-butanediol is possible. For example, via distillation acetoin (boiling point at 148 °C) can be separated from 2,3-butanediol (boiling point at 183 °C) together with the process water. ...
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Improved xylose tolerance and 2,3-butanediol production of Klebsiella pneumoniae by directed evolution of rpoD and the mechanisms revealed by transcriptomics
... By means K. pneumoniae kpG as the parental strain, the effects of the nine most promising genes on the con- sumption of xylose and the production of 2,3-butanediol were evaluated, where seven gene overexpression ...
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Alleviation of carbon catabolite repression in Enterobacter aerogenes for efficient utilization of sugarcane molasses for 2,3-butanediol production
... cost-effectiveness [18]. In our previous study, disruption of the scrR gene, a transcriptional repressor of the scr regulon for sucrose catabolism, was conducted in Entero- bacter aerogenes for efficient utilization of ...
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Thermophilic fermentation of acetoin and 2,3-butanediol by a novel Geobacillus strain
... Background: Acetoin and 2,3-butanediol are two important biorefinery platform chemicals. They are currently fermented below 40°C using mesophilic strains, but the processes often suffer from bacterial ...
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Production of 2,3-butanediol from glucose and cassava hydrolysates by metabolically engineered industrial polyploid Saccharomyces cerevisiae
... The emerging platform materials of C2–C4 diol iso- mers have been studied for production from renew- able resources. Especially, 2,3-butanediol (2,3-BDO) is a promising chemical with various industrial ...
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A newly isolated Bacillus licheniformisstrain thermophilically produces 2,3-butanediol, a platform and fuel bio-chemical
... Results: Bacillus licheniformis strain 10-1-A was newly isolated for 2,3-BD production. The optimum temperature and medium pH were 50°C and pH 7.0 for 2,3-BD production by strain 10-1-A. The medium composition was ...
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Production of 2,3-butanediol in Saccharomyces cerevisiae by in silico aided metabolic engineering
... Butanediol production has been favored over acetoin and diacetyl in beer and wine fermentation due to its neutral organoleptic characteristics. Diacetyl production in brewing process is undesirable due to its ...
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Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method
... Background: Acetoin (AC) and 2,3-butanediol (2,3-BD) as highly promising bio-based platform chemicals have received more attentions due to their wide range of applications. However, the non-efficient substrate ...
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Engineered E. coli W enables efficient 2,3-butanediol production from glucose and sugar beet molasses using defined minimal medium as economic basis
... Following successful shake flask screenings, the most promising strain-construct combination E. coli W/445_ Ediss (445_Ediss) was used as a sound basis for the development of an efficient 2,3-butanediol production ...
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Production of 2, 3 butanediol from rennet whey permeate by Klebsiella pneumoniae immobilized in alginate gel : a thesis presented in partial fulfilment of the requirements for the degree of Master of Technology in Biotechnology at Massey University
... Page Effect of lactose concentration during growth on subsequent 2,3-butanediol production by immobilized cells incubation lactose-supplementation whey permeate... Summary of maximum but[r] ...
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Production of (2R, 3R)-2,3-butanediol using engineered Pichia pastoris: strain construction, characterization and fermentation
... Background: 2,3-butanediol (2,3-BD) is a bulk platform chemical with various potential applications such as aviation fuel. 2,3-BD has three optical isomers: (2R, 3R)-, (2S, 3S)- and meso-2,3-BD. Optically pure ...
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Engineering Corynebacterium glutamicum for the production of 2,3-butanediol
... Background: 2,3-Butanediol is an important bulk chemical with a wide range of applications. In bacteria, this metab- olite is synthesised from pyruvate via a three-step pathway involving α-acetolactate synthase, ...
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Efficient bioconversion of 2,3-butanediol into acetoin using Gluconobacter oxydans DSM 2003
... supplemented with 10 g/L 2,3-butanediol. The flask ex- periment was conducted in 300 mL shake flasks contain- ing 50 mL fresh medium for 12 hours at 200 rpm and 30°C. As shown in Figure 2, 9.6 g/L acetoin was ob- ...
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Deletion of meso-2,3-butanediol dehydrogenase gene bud C for enhanced D-2,3-butanediol production in Bacillus licheniformis
... The strain B. licheniformis , WX-02, used in this study was previously isolated for the production of γ-PGA with 2,3-butanediol and acetoin being the co-products. This strain can grow in a simple medium containing ...
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Engineering Bacillus licheniformis for the production of meso-2,3-butanediol
... Many microorganisms are capable of producing 2,3- BD, such as Enterobacter, Klebsiella, Serratia, and Bacillus. Among them, Bacillus licheniformis is a prom- ising chassis strain for 2,3-BD production. It is generally ...
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Metabolic engineering of Zymomonas mobilis for 2,3-butanediol production from lignocellulosic biomass sugars
... Results: 2,3‑BDO biosynthesis pathways from various bacterial species were examined, which include three genes encoding acetolactate synthase, acetolactate decarboxylase, and butanediol dehydrogenase. ...
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Effect of the inactivation of lactate dehydrogenase, ethanol dehydrogenase, and phosphotransacetylase on 2,3-butanediol production in Klebsiella pneumoniae strain
... Table 3, the yield of ethanol was very high when maltose was used as the substrate in both mutants and wild-type strains, and 2,3-BD production was not the highest, because the ethanol formation path- way competed ...
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Purification of 2,3-butanediol from fermentation broth: process development and techno-economic analysis
... Figure 3 describes a detailed procedure of solvent selec- tion. In general, a systematic approach to selecting the suitable solvent for a certain component is to first deter- mine the solvent type and the ...
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Exotic glycerol dehydrogenase expressing Escherichia coli increases yield of 2,3-butanediol
... varied significantly in different growth phases (Quin- tero et al. 2009), all bacterial cells used for RNA isola- tion were cultured for a constant period to minimize its effect on RT-PCR analysis. It was undoubtedly ...
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