37 C
UGD1, Encoding the Cryptococcus neoformans UDP-Glucose Dehydrogenase, Is Essential for Growth at 37°C and for Capsule Biosynthesis
... We report the identification and disruption of the Cryptococcus neoformans var. grubii UGD1 gene encoding the UDP-glucose dehydrogenase, which catalyzes the conversion of UDP-glucose into UDP-glucuronic acid. Deletion of ...
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Revisiting the issue: can the reading for serologic reactivity following 37°C incubation be omitted?
... STUDY DESIGN AND METHODS: Using data from prior publications, actual risk calculations ARCs were made to predict the risk of eliminating the 37°C reading, pretransfusion direct antiglobu[r] ...
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Clinical Impact of Preincubation of Blood Cultures at 37°C
... at 37°C on the turnaround time and the impact of Gram stain results on antimicrobial management were ...at 37°C until transportation to the ...at 37°C resulted in a 15-h ...
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Transition to 37°C reveals importance of NADPH in mitigating oxidative stress in stored RBCs
... at 4°C with the PIPA solution partially preserved NADPH after raising the temperature of stored RBCs to 37°C, indicating enhanced activity of the PPP. In the presence of the PIPA solution, osmotic ...
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Structural Changes in Dengue Virus When Exposed to a Temperature of 37°C
... Structural change in flavivirus at elevated temperature has been indirectly observed through various antibody-mediated neutral- ization studies, especially in DENV and West Nile viruses (WNV). The epitopes identified by ...
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Investigation of Imperata sp. as a Primary Feedstock for Compost Production in Ucayali region, Peru
... compost C-30, C-37, C-40, C-44 and C-50, ...compost C-30, C-37, C-40, C-44 and C-50, ...composts C-40, C-44, and ...
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Impact of trichloroethylene exposure on the microbial diversity and protein expression in anaerobic granular biomass at 37 degrees C and 15 degrees C
... ◦ C for 10 min; 30 cycles of: 95 ◦ C for 60 s, 55 ◦ C for 60 s, 72 ◦ C for 60 s; followed by a final extension at 72 ◦ C for 10 ...at 37 ◦ C for 12–16 ...
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Studies on the Impact of Thermal Stress on Survival and Development of Adaptive Thermotolerance in Immature Stages of Anopheles culicifacies
... in thermostatic controlled water bath at different temperatures viz. 37˚C, 39˚C, 41˚C, 43˚C and 45˚C for different time periods. Selection of temperatures was based on a simple ...
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Glucose initially inhibits and later stimulates blood ROS generation
... ml resulted in a 200 mM stem solution) in 0.9% NaCl, and 10 µl 36 µg/ml zymosan A (ZyA; Sigma, Deisen- hofen, Germany; article nr. Z-4250-1G, lot nr. 27H0495), final conc.: 1.9 µg/ml. The plates were measured within 0 - ...
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Virion Architecture Unifies Globally Distributed Pleolipoviruses Infecting Halophilic Archaea
... The irregular organization of the VP4 spikes in HRPV-1 indi- cates that there are only weak lateral spike-to-spike interactions, giving rise to a “sloppy” assembly compared to what has been seen, for instance, for ...
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16S rRNA gene pyrosequencing of reference and clinical samples and investigation of the temperature stability of microbiome profiles
... To explore whether the observations for the swabs spiked with the mock bacterial community cells were relevant for respiratory microbiome studies, we collected oropharyngeal samples from eight healthy volunteers. For ...
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Differential effects of acetylcholine on coronary flow in isolated hypothermic hearts from rats and ground squirrels
... In contrast to the rat, ground squirrel coronary vessels are relatively unresponsive to ACh. Their response, if any, was dilation rather than constriction. This effect only became significant at doses of ACh exceeding 5 ...
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Incubation of Whole Blood at 39°C Augments Gamma Interferon (IFN-γ)-Induced Protein 10 and IFN-γ Responses to Mycobacterium tuberculosis Antigens
... (below 37°C) and heat shock temperature (41 to 43°C) conditions have been shown to impair immune cell function (18, 27, 35, 37), a num- ber of studies suggest that incubation at fever-range ...
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Growth Inhibition of Foodborne Pathogens in Camel Milk: Staphylococcus aureus, Listeria monocytogenes Salmonella spp. and E. coli O157:H7
... at 37°C for 48 h, typical black colonies were ...at 37°C for 24 h, typical colourless colonies of ...at 37°C for 24 h, typical black colonies were ...
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Sodium arsenite and hyperthermia modulate cisplatin-DNA damage responses and enhance platinum accumulation in murine metastatic ovarian cancer xenograft after hyperthermic intraperitoneal chemotherapy (HIPEC)
... MMR DNA damage recognition protein. Western blot analysis of p53, XPC, XPA, ERCC1 and MSH2 revealed mouse-to-mouse and tumor-to-tumor variabilities (Figure 4A). Some tumors failed to express the protein of interest while ...
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Cryptococcus neoformans Dual GDP-Mannose Transporters and Their Role in Biology and Virulence
... at 37°C, protein N-glycosy- lation, and capsule synthesis all rely more heavily on the activity of Gmt1 and are dramatically impaired in the absence of both pro- teins (see ...
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Low temperature anaerobic digestion is associated with differential methanogenic protein expression
... microbial diversity and protein expression in anaerobic granular biomass at 37°C and 15°C... Syutsubo K, Yoochatchaval W, Yoshida H, et al.[r] ...
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Conformational change of the coronavirus peplomer glycoprotein at pH 8.0 and 37 degrees C correlates with virus aggregation and virus-induced cell fusion.
... The aggregation of virions and the isolated E2 S glycoprotein at pH 8.0 and 37°C or following treatment with guanidine and urea at pH 6.0 and 37°C indicate that an irreversible conformat[r] ...
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In Vivo and In Vitro Anaerobic Mating in Candida albicans
... Stability of opaque cells at 37°C under anaerobic conditions. Our goal was to determine whether the opaque cells used in the in vitro anaerobic mating ex- periments maintained an opaque cell-specific gene ...
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Expression of Alpha-Crystalline Protein in the In- Vitro Model of Hypoxically Induced Nonreplicating Persistence of Mycobacterium Tuberculosis Strains
... Culture for inocula seed preparation: The experimental strains (each experimental set contains 10 strains) of MTB inoculated in to the sterile culture tubes containing DTA and 8 mm long Teflon-coated stirring bar. The ...
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