3D-Polymerase
Transgenic Expression of the 3D Polymerase Inhibits Theiler's Virus Infection and Demyelination
... expressed 3D may have interfered with virus-expressed ...that 3D oligomerizes with itself and other picornavirus proteins to efficiently transcribe the RNA ...Transgenic 3D may lose its ability to ...
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Enterovirus 71 VPg Uridylation Uses a Two-Molecular Mechanism of 3D Polymerase
... EV71 belongs to the Enterovirus genus, family Picornaviridae. The virus possesses a 7,400-nucleotide single-stranded, positive- sense RNA genome (9, 12). The genomic RNA contains long 5= untranslated regions (5=-UTR), a ...
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Promyelocytic Leukemia Isoform IV Confers Resistance to Encephalomyocarditis Virus via the Sequestration of 3D Polymerase in Nuclear Bodies
... Promyelocytic leukemia (PML) protein is the organizer of nuclear matrix-associated nuclear bodies (NBs), and its conjugation to the small ubiquitin-like modifier (SUMO) is required for the formation of these structures. ...
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Genetic dissection of interaction between poliovirus 3D polymerase and viral protein 3AB.
... The plasmid pJG4-5 encodes the TRP1 marker and is the parent plasmid for the prey constructs. Under the control of the galactose-dependent GAL1 pro- moter, it encodes the B42 transcriptional activation domain from E. ...
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Both cis and trans Activities of Foot-and-Mouth Disease Virus 3D Polymerase Are Essential for Viral RNA Replication
... that 3D cannot assemble into the replication complex alone but is initially assembled as part of a larger precursor, which is subse- quently processed to the active 3D component ...active 3D and/or ...
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Both cis and trans Activities of Foot-and-Mouth Disease Virus 3D Polymerase Are Essential for Viral RNA Replication
... Replication-defective 3D point mutations do not affect P3 ...to 3D were a result of protein modification and not local RNA changes, we sought to identify whether such mutations af- fected 3D ...
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Coupled translation and replication of poliovirus RNA in vitro: synthesis of functional 3D polymerase and infectious virus.
... Overall, the kinetics of poliovirus replication protein synthesis, polymerase activity, and virus production observed in the HeLa S10-initiation factor in vitro translation reactions wer[r] ...
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Human Rhinovirus 87 and Enterovirus 68 Represent a Unique Serotype with Rhinovirus and Enterovirus Features
... 187, 188, 189, and 222 (21). Part of the 3D polymerase gene was amplified with the primers 3D⫹ and 3D⫺ (24). The PCR products were visualized under UV light after electrophoresis on a 2% ...
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Ribavirin-Resistant Mutants of Human Enterovirus 71 Express a High Replication Fidelity Phenotype during Growth in Cell Culture
... the 3D polymerase is situated on a three- stranded -sheet scaffold located in the palm region, in which four conserved aspartate residues are thought to facilitate catalysis ...poliovirus 3D ...
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SUMO Modification Stabilizes Enterovirus 71 Polymerase 3D To Facilitate Viral Replication
... the 3D protein, an RNA-dependent RNA polymerase (RdRp) of EV71, is modified by small ubiquitin-like modifier 1 (SUMO-1) both during infection and in ...for 3D SUMOylation as determined by ...
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Allosteric Effects of Ligands and Mutations on Poliovirus RNA-Dependent RNA Polymerase
... 2C RNA for all other figures], 0.5 M 3CD where indicated, 3AB as indicated, and 1 M 3D polymerase (wild-type or mutant poliovirus or wild-type coxsack- ievirus). Reactions were incubated at 30°C for 2 h. ...
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Characterization of Protein-Protein Interactions Critical for Poliovirus Replication: Analysis of 3AB and VPg Binding to the RNA-Dependent RNA Polymerase
... of 3D polymerase necessary for ITC analysis, the highly soluble mu- tant 3D-DD was used ...wild-type 3D (40; ...the polymerase recruitment assay using membranes containing a 3AB ...
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3D CT Arteriography and 3D CT Venography: The Separate Demonstration of Arterial Phase and Venous Phase on 3D CT Angiography in a Single Procedure
... on 3D-CT angiog- raphy, the correct identification of the different ves- sels depends on the experience and subjective judg- ment of the investigator who identified arteries or veins, introducing the possibility ...
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Environmental adaptability and stress tolerance of Laribacter hongkongensis: a genome-wide analysis
... , is responsible for recognizing the promo- ters for transcription of most of the housekeeping genes. Furthermore, the L. hongkongensis genome contains other alternative s -factors, including s 28 (FliA), s 32 (RpoH), s ...
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Translational regulation of herpes simplex virus DNA polymerase.
... Analysis of the transcript of the herpes simplex virus DNA polymerase gene provides evidence that polymerase expression is inefficient at the level of translation.. The kinetics of expre[r] ...
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Nested polymerase chain reaction technique for the detection of Gpc3 and Afp mRNA in liver cancer micrometastases.
... The incidence of liver cancer in China has gradually increased. The rate of liver cancer recurrence is still high despite various procedures and therapies (Lemoine et al., 1997). Research has shown that the 5-year ...
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Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis
... can either be present in the biological sample or be in- troduced during preparation or the sampling process. Especially in resource-limited settings, a uniform sam- pling and handling might not be guaranteed. The ...
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Specific Serological Relationships Among Partially Purified DNA Polymerases of Avian Leukosis-Sarcoma Viruses, Reticuloendotheliosis Viruses, and Avian Cells
... For example, IgGRSV-RAV-0 did not neutralize TDSNV DNA polymerase activity, and IgG-TDSNV only slightly neutralized RSV-RAV-0 DNA polymerase activity, whereas RSV-RAV-0 DNA polymerase wa[r] ...
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An ordered topological representation of 3D triangular mesh facial surface: concept and applications
... In this article, we presented a unified framework for ana- lyzing, describing, and encoding 3D triangular mesh facial surfaces. We proposed a novel representation that is sim- ple, compact, generic and ...
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Comparison Between Real-Time Polymerase Chain Reaction and DNA-Microarray in Detection and Identification of Mycobacterium Species
... DNA amplification was carried out in a final volume of 50 μl, with 0.4 μM of each primer, 200 μM of each dNTPs, 10 mM Tris HC1, pH 8.3, 50 mM KC1; 0.1 % triton X-100, 1 U of Taq-polymerase (Pharmacia) and 5 μl of ...
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