3H-Thy, 3H-Thymidine
Biochemical and electron microscopic studies of the replication and composition of milker's node virus.
... Symbols: *, Incorporation of[3H]thymidine into acid-precipitable material in the cytoplasm of uninfected cells; 0, incorporation of [3H]thymidine into acid-precipitable material in the c[r] ...
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Evidence for a mouse mesangial cell derived factor that stimulates lymphocyte proliferation
... The functions of the glomerular mesangium are served by at least two populations of cells-- a cell bearing microfilaments that regulates blood flow, and a phagocytic cell bearing Ia determinants and Fc receptors. We ...
6
Epstein-Barr virus uses HLA class II as a cofactor for infection of B lymphocytes.
... However, using Akata strain virus radiolabeled with [3H]thymidine, we found no evidence for the ability of HB55, the other anti-HLA antibodies, or antibody to gp42 to inhibit significant[r] ...
6
Disruption of Adenovirus Type 7 by Lithium Iodide Resulting in the Release of Viral Deoxyribonucleic Acid
... When 3H-thymidine-labeled adenovirus type 7 was treated at 36 C with solutions of increasing concentration and submitted to rate zonal centrifugation, the peak of radioactive label recov[r] ...
6
Prostaglandin Suppression of Mitogen Stimulated Lymphocytes In Vitro: CHANGES WITH MITOGEN DOSE AND PREINCUBATION
... In our initial report of the PG-producing suppressor cell, we had found that the percent increase in [3H]thymidine incorporation caused by indomethacin in PHA-stimulated leukocyte cultur[r] ...
9
Biochemical mechanisms in the Killmann experiment: critique of the deoxyuridine suppression test
... intracellular deoxyuridine. Inhibition of thymidine kinase activity by both mebolites further decreases the specific radioactivity of [3H]dTMP and [3H]dTTP. Deoxyuridine also inhibits the ...
13
Nature and specificity of the immune response to collagen in type II collagen induced arthritis in mice
... When spleen cells from arthritic mice were cultured in the presence of native type II collagen, increased incorporation of [3H]thymidine occurred with increasing collagen concentrations [r] ...
12
The angiotensin AT2 receptor mediates inhibition of cell proliferation in coronary endothelial cells
... Angiotensin II (ANG II) is known to be a potent growth promoting factor for vascular smooth muscle cells and fibroblasts but little is known about its influence on growth in endothelial cells. We studied the effects of ...
8
Antibody based targeting of FGFR3 in bladder carcinoma and t(4;14) positive multiple myeloma in mice
... Figure 5 R3Mab inhibits proliferation, clonal growth, and FGFR3 signaling in bladder cancer cells expressing WT or mutated FGFR3S249C.. A Inhibition of [3H]thymidine incorporation by R3M[r] ...
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PURIFICATION AND CHARACTERIZATION OF THE CLONED HUMAN GM-CSF GENE EXPRESSED IN ESCHERICHIA COLI
... Immunoaffinity chromatography was used for purification of GM-CSF. A column was constructed which anti-GM-CSF was coupled to CNBr-activated Sepharose 4B. The renatured protein was loaded on the column and eluted by the ...
6
Inhibition of lymphoproliferation by hyperlipoproteinemic plasma
... Plasma for patients with primary type IV or V hyperlipoproteinemia inhibited [3H]thymidine incorporation by cultured mononuclear leukocytes. This previously unreported abnormality affected mononuclear ...
6
Role of local prostaglandin synthesis in the modulation of proliferative activity of rat colonic epithelium
... The role of local prostaglandin (PG) synthesis in the modulation of the proliferative activity of colonic epithelium was examined in rat colon. Experimental rats were given either indomethacin (5 mg/kg s.c. every 8 h for ...
12
Inhibition of immune functions by antiviral drugs
... content, and [3H]thymidine uptake were decreased in cultures treated with AZT, ribavirin, or ganciclovir. AZT or ribavirin but not ganciclovir reduced RNA and protein in the cultures and inhibited cell ...
10
Proliferation related expression of p19/nm23 nucleoside diphosphate kinase
... The increase in p19/nm23 in response to mitogenic stimulation of lymphocytes, which parallels the increase in [3H]thymidine uptake and in the percentage of cells in S phase, together wit[r] ...
7
Early Events in the Infection of Permissive Cells with Simian Virus 40: Adsorption, Penetration, and Uncoating
... To determine whether intact virions penetrate the nuclei, AGMK cells were infected with 3H-thymidine- or 3H-leucine-labeled SV40 and were harvested at intervals after infection; their nu[r] ...
9
Insulin like growth factor I mediates selective anabolic effects of parathyroid hormone in bone cultures
... PTH was studied for its effects on bone formation in cultured rat calvariae. 0.01-10 nM PTH stimulated [3H]thymidine incorporation into DNA by up to 4.8-fold. Although continuous treatment with PTH for ...
7
Low density lipoprotein enhances the cellular action of arginine vasopressin in rat glomerular mesangial cells in culture
... The present study was undertaken to determine whether low density lipoprotein (LDL) modulates the cellular action of arginine vasopressin (AVP) in rat glomerular mesangial cells in culture. AVP increased cellular free ...
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Retrobulbar T cells from patients with Graves' ophthalmopathy are CD8+ and specifically recognize autologous fibroblasts
... Proliferation [3H]thymidine incorporation of T cell lines in response to stimulation with irradiated autologous retrobulbar fibroblasts U, irradiated autologous retrobulbar fibroblasts i[r] ...
7
Exclusion of bacteriophage T1 by bacteriophage lambda. II. Synthesis of T1-specific macromolecules under N-mediated excluding conditions.
... gene products data not DISCUSSION From DNA-DNA hybridization experiments with DNA extracted from excluding and control cultures labeled with [3H]thymidine by either prelabeling or contin[r] ...
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Intracellular forms of simian virus 40 nucleoprotein complexes. V. Enrichment for "active" simian virus 40 chromatin by differential precipitation with Mg2+.
... The fraction containing the Mg2+-insoluble SV40 chromatin was found also to be enriched for the following: i DNA replication intermediates as analyzed by short pulse with [3H]thymidine; [r] ...
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