[3H]Thymidine incorporation
![Nonradioactive Techniques for Measurement of In Vitro T-Cell Proliferation: Alternatives to the [3H]Thymidine Incorporation Assay](data:image/gif;base64,R0lGODlhAQABAIAAAP///wAAACH5BAEAAAAALAAAAAABAAEAAAICRAEAOw==)
Nonradioactive Techniques for Measurement of In Vitro T-Cell Proliferation: Alternatives to the [3H]Thymidine Incorporation Assay
... T-cell proliferation is an important in vitro parameter of in vivo immune function and has been used as a prognostic marker of human immunodeficiency virus type 1 (HIV-1) disease progression. The proliferative capacity ...
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Antibody based targeting of FGFR3 in bladder carcinoma and t(4;14) positive multiple myeloma in mice
... Figure 5 R3Mab inhibits proliferation, clonal growth, and FGFR3 signaling in bladder cancer cells expressing WT or mutated FGFR3S249C.. A Inhibition of [3H]thymidine incorporation by R3M[r] ...
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Immunosuppression by D penicillamine in vitro Inhibition of human T lymphocyte proliferation by copper or ceruloplasmin dependent generation of hydrogen peroxide and protection by monocytes
... Although the work presented here examined the effect of D-penicillamine and CuS04 on mitogen-induced T cell [3H]thymidine incorporation, previous studies have indicated that helper T cel[r] ...
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PURIFICATION AND CHARACTERIZATION OF THE CLONED HUMAN GM-CSF GENE EXPRESSED IN ESCHERICHIA COLI
... Immunoaffinity chromatography was used for purification of GM-CSF. A column was constructed which anti-GM-CSF was coupled to CNBr-activated Sepharose 4B. The renatured protein was loaded on the column and eluted by the ...
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Biochemical mechanisms in the Killmann experiment: critique of the deoxyuridine suppression test
... of thymidine kinase activity by both mebolites further decreases the specific radioactivity of [3H]dTMP and ...the incorporation of [3H]deoxyadenosine and [3H]deoxyguanosine into DNA in ...
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The angiotensin AT2 receptor mediates inhibition of cell proliferation in coronary endothelial cells
... Angiotensin II (ANG II) is known to be a potent growth promoting factor for vascular smooth muscle cells and fibroblasts but little is known about its influence on growth in endothelial cells. We studied the effects of ...
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Evaluation of the anti angiogenic properties of the new selective αVβ3 integrin antagonist RGDechiHCit
... Figure 3 In vitro effects of cRGDf[NME]V and RGDechiHCit on vascular smooth muscle cell VSMC cell proliferation Panel A and DNA synthesis assayed by [3H]thymidine incorporation Panel B..[r] ...
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Inhibition of lymphoproliferation by hyperlipoproteinemic plasma
... lipoproteins in appreciable amounts. [3H]-Thymidine incorporation correlated well with morphologic evidence of lymphoproliferation. The mechanism of the inhibitory effect of type IV or V ...
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Low density lipoprotein enhances the cellular action of arginine vasopressin in rat glomerular mesangial cells in culture
... [3H]thymidine incorporation, an effect significantly greater in the presence of 10 micrograms/ml ...[3H]thymide incorporation and mitogen-activated protein kinase were not altered by 10 ...
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Stimulation of the proliferation of human bone cells in vitro by human monocyte products with interleukin 1 activity
... [3H]thymidine incorporation and in cell number, we found that human monocytes in culture released factors capable of stimulating the proliferation of osteoblast-like cells derived from human ...
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Insulin like growth factor I mediates selective anabolic effects of parathyroid hormone in bone cultures
... [3H]thymidine incorporation into DNA by up to ...[3H]proline incorporation into collagen, transient (24 h) treatment enhanced [3H]proline incorporation into collagen 24-48 ...
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Retrobulbar T cells from patients with Graves' ophthalmopathy are CD8+ and specifically recognize autologous fibroblasts
... Proliferation [3H]thymidine incorporation of T cell lines in response to stimulation with irradiated autologous retrobulbar fibroblasts U, irradiated autologous retrobulbar fibroblasts i[r] ...
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Regulation of proliferation of human colonic subepithelial myofibroblasts by mediators important in intestinal inflammation
... We next studied the possibility that the mitogenic effects of growth factors may be negatively regulated by VIP. It is un- likely that proliferative stimuli act unopposed in vivo. In myo- fibroblast-like cells derived ...
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Proliferation related expression of p19/nm23 nucleoside diphosphate kinase
... The increase in p19/nm23 in response to mitogenic stimulation of lymphocytes, which parallels the increase in [3H]thymidine uptake and in the percentage of cells in S phase, together wit[r] ...
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Early Events in the Infection of Permissive Cells with Simian Virus 40: Adsorption, Penetration, and Uncoating
... To determine whether intact virions penetrate the nuclei, AGMK cells were infected with 3H-thymidine- or 3H-leucine-labeled SV40 and were harvested at intervals after infection; their nu[r] ...
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Carbohydrate deficient glycoprotein syndrome type Ib Phosphomannose isomerase deficiency and mannose therapy
... to this paper. Secondary authors G. Alton and C. Körner contributed equally. H.K. Harms and K. Reiter took care of the patient, initiated the mannose therapy, and were instrumental in the cooperation with the other ...
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Antibacterial properties of diterpenes and their derivatives : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Microbiology at Massey University
... Thymidine incorporation per microgram protein by MRSA strain MR96/l 64 in the presence of increasing concentrations of totarol.. Glutamine incorporation per viable cell by MRSA strain MR[r] ...
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Evidence supporting the identity in Graves' disease of thyroid stimulating antibody and thyroid growth promoting immunoglobulin G as assayed in FRTL5 cells
... This paper addresses the question: in Graves' disease is there a thyroid-growth stimulating IgG (TGI) separate from thyroid-stimulating antibody (TSAb)? Using the functioning rat thyroid line (FRTL5) cells for TGI ...
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Cytotoxic Effect of Dexamethasone Restricted to Noncycling, Early G1 Phase of Melanoma Cells
... This study deals with the inhibitory effects of dexamethsone on the proliferation of a human melanoma cells after sepa- ration into different fractions according to their position in the cell cycle using a gravity ...
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Immunological studies of aging Decreased production of and response to T cell growth factor by lymphocytes from aged humans
... tritiated thymidine (3H-Tdr) than did similar cultures from young donors in the presence of either ...tritiated thymidine incorporation when incubated with exogenous human ...tritiated ...
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