5-aza
Epigenetic Inheritance of 5-AZA-2'-Deoxycytidine (5-AZA-CdR) Induced Alterations
... 67 rats demasculinizes and feminizes male offspring due to its antiandrogenic activity [36]. The effects of Di-n-butyl phthalate (DBP) on male reproductive tract development include missing epididymis and vasa ...
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A subgroup of pancreatic adenocarcinoma is sensitive to the 5-aza-dC DNA methyltransferase inhibitor
... [9]. 5-aza-dC demonstrates activity against hematologic malignancy [10] and is used as first-line of treatment in AML patients > 65 years who are not candidates for intensive chemotherapy [11], whereas ...
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AID downregulation is a novel function of the DNMT inhibitor 5-aza-deoxycytidine
... p21. 5-aza-CdR inhibited nuclear AID expression through ubiquitin-proteasome degradation, thus exerting a cytotoxic effect in AID-positive hematopoietic ...
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Gene Expression Profiling of Human Myeloid Leukemic MV4 11 Cells Treated with 5 Aza 2’ deoxycytidine
... of 5-aza-dC, gene expres- sion profiles of MV4-11 cells treated with the drug were examined and compared to non-treated cells as described in the Methods ...following 5-aza-dC treatment are ...
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5-Aza-2′-deoxycytidine stress response and apoptosis in prostate cancer
... with 5- azadC overexpressed Bcl2 and DNMT1 in prostate cancer cell lines TSUPr1, DuPro, and PC3 dose ...the 5- azadC-treated TSUPr1 and other cells is characteristic for apoptotic cells, a biochemical ...
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Effects of 5 aza 2’ deoxyctidine on the development of porcine parthenogenetic and nuclear transfer embryos
... to 5 nM 5-aza- dC for 1 hour promotes further development by trigger- ing adjustment of methylation status without injuring ...with 5 nM 5-aza-dC for 1 to 20 hours (Table ...of ...
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Low-Dose 5-Aza-2′-deoxycytidine Pretreatment Inhibits Experimental Autoimmune Encephalomyelitis by Induction of Regulatory T Cells
... 10-d 5-Aza could prevent autoimmune disease in the CNS of a murine EAE model (Figure ...short-term 5-Aza-pretreated MOG- induced mice developed EAE during our observation period (Figure ...
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The Effect of 5-Aza-2′-Deoxycytidine in Combination to and in Comparison with Vorinostat on DNA Methyltransferases, Histone Deacetylase 1, Glutathione S-Transferase 1 and Suppressor of Cytokine Signaling 1 Genes Expression, Cell Growth Inhibition and Apop
... are reversible and could be modified in response to DNMT and HDAC inhibitors. Because histone deacetylation and DNA methylation are the two best-characterized covalent modifications associated with chromatin compaction ...
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Venetoclax with azacitidine targets refractory MDS but spares healthy hematopoiesis at tailored dose
... using 5-AZA and venetoclax in patients with MDS/ sAML, overall in “HMA-failure” ...lower-dose 5-AZA shows a valuable cytotoxicity on the malignant cell when combined with ...of ...
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Antileukemic activity of combined epigenetic agents, DNMT inhibitors zebularine and RG108 with HDAC inhibitors, against promyelocytic leukemia HL-60 cells
... as 5-azacytidine (Vidaza) and 5-aza-2'-deoxycytidine (Decitabine), have been used clinically in the treatment of hematological malignancies, including the myelodysplastic syndrome and acute leukemia ...
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Insulin like growth factor binding protein 7 (IGFBP7) expression is linked to poor prognosis but may protect from bone disease in multiple myeloma
... Results: Median IGFBP7 expression is significantly lower in CD138-purified plasma cells from individuals with MGUS and MM, compared to normal bone marrow plasma cells. IGFBP7 gene expression in MM cells is regulated by ...
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Epigenetic therapy of myelodysplastic syndromes connects to cellular differentiation independently of endogenous retroelement derepression
... as 5- azacytidine (5-AZA) and 5-aza-2′-deoxycytidine (decita- bine), have been the mainstay of treatment for higher- risk MDS and also for older, unfit AML ...both 5-AZA ...
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Ovarian cancer plasticity and epigenomics in the acquisition of a stem-like phenotype
... inhibitors 5-aza-dC, decitabine and zebularine show promise in reversal of repressive histone mark pat- terns and resensitization of ovarian cancer cells to chemo- therapy [127,128], and several are ...
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Circulating cell free DNA based epigenetic assay can detect early breast cancer
... -Aza-dC: 5 ’ -Aza-2-deoxycytidine; AUC: area under the curve; BC: breast cancer; BORN: Breast Oncology Research Network; cfDNA: circulating cell-free DNA; CNA: copy number alteration; CPXM1: ...
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Original Article miR-375 is downregulated by promoter hypermethylation in MIN6 insulinoma cells
... 5-Aza-CdR treatment. There is a negative cor- relation between DNA methylation levels and relative expression levels of mmu-miR-375. Although MIN6 cells belong to the tumor cells can proliferate ...
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Generation of tumor initiating cells by exogenous delivery of OCT4transcription factor
... three-dimensional; 5-aza-2 ’ dC: 5-aza-2 ’ -deoxycytidine; CDH1: E-cadherin; CK: cytokeratin; CTG: Cell Titer Glo; DMEM: Dulbecco ’ s modified Eagle ’ s medium; EMT: epithelial-to-mesenchymal ...
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Interpreting clinical assays for histone deacetylase inhibitors
... Abbreviaions: 5-aza, 5-aza-cytidine; 5-azaDc, 5-aza-2′-deoxycytidine; 5-FU, 5-fluorouracil; 13-cis-RA, 13-cis-retinoic acid; ALL, acute lymphoblastic ...
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Integrin 9gene promoter is hypermethylated and downregulated in nasopharyngeal carcinoma
... Epigenetic silencing of tumor suppressor genes (TSGs) by promoter methylation can be an early event in the multi-step process of carcinogenesis. Human chromosome 3 contains clusters of TSGs involved in many cancer types ...
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Methylation status of insulin-like growth factor-binding protein 7 concurs with the malignance of oral tongue cancer
... in 5 ′ region of IGFBP-7 by methylation-specific PCR ...with 5-AZA-2 ′ deoxycytidine, the de-methylated CpG sites in the 5 ′ region of IGFBP-7 were observed and IGFBP-7 mRNA expression was ...
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Epigenetic silencing of TGFBI confers resistance to trastuzumab in human breast cancer
... Additional file 2: Figure S1. DNA methylation-associated silencing of CXCL2 and SLC38A1 comparing the trastuzumab-resistant (AUTR) and -sensitive (AU) AU565 cell model. (A) Expression levels of CXCL2 and SLC38A1 in the ...
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