5′-Cap dependent translation
Cap-Independent Translation Conferred by the 5′ Leader of Tobacco Etch Virus Is Eukaryotic Initiation Factor 4G Dependent
... a 5⬘ cap or the TEV IRES are not able to confer a translational advantage to an ...a 5⬘ cap or the TEV IRES under conditions of competitive translation where the factor is present in ...
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Blocking eIF4E-eIF4G Interaction as a Strategy To Impair Coronavirus Replication
... capped 5 ⴕ leader sequence. The translation of most of the sgmRNAs is thought to be cap dependent and displays a requirement for eukaryotic initiation factor 4F (eIF4F), a heterotrimeric ...
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THE WARBURG EFFECT: A POSSIBLE ROLE OF CAP INDEPENDENT TRANSLATION Ajaz Ahmad Waza*, Shabir Ahmad Bhat, Sajad AliDOWNLOAD/VIEW
... utilizes cap- dependent mechanism for translation ...the 5’- m7GpppN cap on the ...the 5′ end of the mRNA as a 43S complex (40S, eIF1, eIF2, eIF3, eIF5, eIF1A and ...the ...
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Alpha/Beta Interferon Inhibits Cap-Dependent Translation of Viral but Not Cellular mRNA by a PKR-Independent Mechanism
... the translation of the electroporated SB reporter was similar in wild-type and PKR ⫺/⫺ MEFs, ranging from 5- to ⬎ 20-fold depending upon the experiment ...contrast, translation directed by the EMCV ...
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A Viral Noncoding RNA Generated by cis-Element-Mediated Protection against 5′→3′ RNA Decay Represses both Cap-Independent and Cap-Dependent Translation
... the 5 ⬘ end of ...the 5 ⬘ end to just before the first guanine residue in the Seq1f58 is 962, and the number from the first guanine residue in the Seq1f58 to the 3 ⬘ end is ...
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<p>INPP4B promotes colorectal cancer cell proliferation by activating mTORC1 signaling and cap-dependent translation</p>
... mediated cap-dependent translation. Importantly, inhibiting cap-dependent translation with 4EGI-1 repressed the ability of INPP4B to promote CRC cell proliferation, while 4E-BP1 ...
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Identification and Characterization of the Functional Elements within the Tobacco Etch Virus 5′ Leader Required for Cap-Independent Translation
... less 5⬘ end dependent than CIRE-2, suggesting that the two elements are functionally ...in cap-inde- pendent translation when both elements are present suggests a concerted mechanism to ...
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A Small Yeast RNA Blocks Hepatitis C Virus Internal Ribosome Entry Site (HCV IRES)-Mediated Translation and Inhibits Replication of a Chimeric Poliovirus under Translational Control of the HCV IRES Element
... IRES-mediated translation was determined in ...by cap-dependent translation, the downstream luciferase synthesis occurs by HCV IRES-mediated ...2B). Translation con- ditions were chosen ...
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Dengue Virus Utilizes a Novel Strategy for Translation Initiation When Cap-Dependent Translation Is Inhibited
... siRNA expression. An eIF4E-specific siRNA construct was generated from the pRF42 plasmid backbone (46). An oligonucleotide encoding sequence from the eIF4E gene (nt ⫺9 to ⫹ 12; GenBank accession no. BC010759) (13) in the ...
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The 5′ Untranslated Region of the Human T-Cell Lymphotropic Virus Type 1 mRNA Enables Cap-Independent Translation Initiation
... vitro translation assays (Fig. 1 through 5) and RNA injections in Xenopus laevis oocytes experiments ...the 5=UTR of the HTLV-1 full-length mRNA contains an ...monocistronic cap-depen- dent ...
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trans complementation of cap-independent translation directed by poliovirus 5' noncoding region deletion mutants: evidence for RNA-RNA interactions.
... Thus, neither the expression of PV 2A in the absence of the PV 5' NCR nor the inhibition of cap-dependent translation by the FMDV L protein appeared to be sufficient to produce the enhan[r] ...
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Activation of Cap-Dependent Translation by Mucosal Human Papillomavirus E6 Proteins Is Dependent on the Integrity of the LXXLL Binding Motif
... Western blotting and antibodies. Unless indicated otherwise, cell lysates were prepared using ML buffer (300 mM NaCl, 0.5% Nonidet P-40 [NP-40], 20 mM Tris-HCl [pH 8.0], 1 mM EDTA) supplemented with one complete ...
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Original Article Expression of eukaryotic initiation factor 4E and 4E binding protein 1 in colorectal carcinogenesis
... a 5’ terminal m7G [5’] ppp [5’] N cap and a 50 to 300 nucleotide long 3’poly (A) tail, cap- dependent translation is the most common mechanism of ...the 5’ UTR ...
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Ribosome Binding to a 5′ Translational Enhancer Is Altered in the Presence of the 3′ Untranslated Region in Cap-Independent Translation of Turnip Crinkle Virus
... during cap-dependent translation (24, 29), allowing for protection of terminal regions of the RNA, recruitment of eIFs, and recycling of ribosomal ...the 5 ⬘ UTR and 3 ⬘ CITE-associated ...
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Translational Control Mediates Lifespan Extension Due to Dietary Restriction in Drosophila
... partially dependent upon ...the translation initiation factor eIF4E, these data suggested that modulation of mRNA translation upon DR may alter the lifespan and metabolism of the ...genome-wide ...
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Role of the PB2 627 Domain in Influenza A Virus Polymerase Function
... lid, cap-binding, mid-link, and 627 domains are needed for the initiation of RNA synthesis, we performed in vitro activity assays using purified recombinant ...the cap snatching and capped RNA primer ...
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Viral Internal Ribosome Entry Site Structures Segregate into Two Distinct Morphologies
... HCV 5⬘ UTR was examined to determine whether domain III of HCV can form independently of domain II, as suggested by X-ray scattering and NMR studies (15, ...driving translation initiation, it does associate ...
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Functional Analyses of RNA Structures Shared between the Internal Ribosome Entry Sites of Hepatitis C Virus and the Picornavirus Porcine Teschovirus 1 Talfan
... Picornavirus genomes are infectious and function as mRNAs. Initiation of protein synthesis on picornavirus RNA is depen- dent on an internal ribosome entry site (IRES) (see reference 2 for a review). Several different ...
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Translation initiation at a downstream AUG occurs with increased efficiency when the upstream AUG is located very close to the 5' cap.
... To determine whether the short length of the untranslated leader of this mRNA affects the efficiency of translation initiation at the LP1 initiation signal, monkey cells were transfected[r] ...
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trans Regulation of Cap-Independent Translation by a Viral Subgenomic RNA
... the 5 ⬘ end (construct D-SG1fLUC) (Fig. 6B). We tested the translation efficiency of D-SG1fLUC relative to SG1fLUC and GfLUC in the presence of 20-fold- excess sgRNA2 and sgRNA2BF in wheat germ extract ...
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