5-methylcytosine
Spontaneous mutation at a 5-methylcytosine hotspot is prevented by very short patch (VSP) mismatch repair.
... A hotspot for spontaneous mutation that corresponds with a 5-methylcytosine was observed in wild-type lysogens but was not present in bacteria lacking both methylase and VSP repair a[r] ...
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Increased 5-hydroxymethylcytosine and decreased 5-methylcytosine are indicators of global epigenetic dysregulation in diffuse intrinsic pontine glioma
... repressive methylcytosine DNA alteration, 5-hydroxymethylation of cytosine (5hmC) is recognized as an epigenetic mark associated with active ...and 5-methylcytosine ...
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<p>Transcriptome-Wide 5-Methylcytosine Functional Profiling of Long Non-Coding RNA in Hepatocellular Carcinoma</p>
... Posttranscriptional RNA modifications have emerged as an additional layer of epigenetic regulation. 1 Over one hundred different chemical modifications in RNA have been described, including pseudouridine, ...
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African Trypanosomes Contain 5-Methylcytosine in Nuclear DNA
... base 5-methylcytosine (5MC) is widespread in prokaryotes and higher eukaryotes, we searched for genes capable of encoding a cytosine-5 DNA methyltrans- ferase (C5-DNA MTase) in ...
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Dynamics of 5-methylcytosine and 5-hydroxymethylcytosine during pronuclear development in equine zygotes produced by ICSI
... cytosine, 5-methylcytosine (5mC), was the first epi- genetic modification discovered in DNA and, hence, is nowadays the best studied DNA epigenetic modifica- ...
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The Lytic Phase of Epstein-Barr Virus Requires a Viral Genome with 5-Methylcytosine Residues in CpG Sites
... of 5-methylcytosine residues in CpG sites of virion DNA prevents the expression of essential lytic genes indispensable for viral DNA amplification during productive ...of 5-methylcytosine ...
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Epigenetic regulatory functions of DNA modifications: 5-methylcytosine and beyond
... mark, 5-methylcytosine, the field garnered increased interest through the recent discovery of additional ...of 5-methylcytosine on CpG dinucleotide as a key feature, because it permits the ...
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Dysregulation and prognostic potential of 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC) levels in prostate cancer
... mark 5-hydroxymethylcytosine (5hmC) has recently been proposed as a novel candidate prognostic biomarker in several malignancies including ...of 5-methylcytosine (5mC) and can be further oxidized to ...
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Distinct and overlapping control of 5 methylcytosine and 5 hydroxymethylcytosine by the TET proteins in human cancer cells
... of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) in an α-ketoglutarate- and Fe(II)-dependent manner ...generate 5-formylcytosine and 5-carboxylcyto- sine (5caC), both of ...
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Enhanced 5-methylcytosine detection in single-molecule, real-time sequencing via Tet1 oxidation
... in 5 ’ -CCWGG-3 ’ sequence contexts are plotted in red, while IPD ratio data for all other contexts is plotted in ...of 5 ’ -CCWGG-3 ’ in the genome. An orthogonal off-target motif (5 ’ -GGWCC-3 ’ ) ...
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Nucleobase, Nucleoside, And Neighboring Nucleotides: Intrinsic Preferences For Tet Enzyme-Mediated Oxidation Of 5-Methylcytosine
... of 5-methylcytosine ...oxidize 5-methyl-2’-deoxycytidine in genomic DNA, yielding products that contribute to epigenetic regulation in mammalian cells, but have since been found to also oxidize ...
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Wilms' Tumor protein 1 and enzymatic oxidation of 5 methylcytosine in brain tumors: potential perspectives
... Approximately 5–20% of Wilms’ Tumors cases exhibit somatic WT1 gene mutations which tend to be sporadic bi- allelic aberrations occurring in blastemal progenitors (Kaneko et ...∼ 5% of WT cases (Ruteshouser ...
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Conserved Domains Required for DEMETER 5-Methylcytosine DNA Glycosylase/lyase Function in planta.
... manufacturer’s instructions. Adaptor-ligated DNA was cleaned up with 1x AMPure XP beads. The purified adaptor-ligated DNA was spiked with 50ng of unmethylated cl857 Sam7 Lambda DNA and subjected to one round of sodium ...
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DNA looping by two 5-methylcytosine-binding proteins quantified using nanofluidic devices
... 21. Reisner W, Beech J, Larsen N, Flyvbjerg H, Kristensen A, Tegenfeldt JO. Nanoconfinement-enhanced conformational response of sin- gle DNA molecules to changes in ionic environment. Phys Rev Lett. ...
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Hyperglycemia affects global 5-methylcytosine and 5-hydroxymethylcytosine in blood genomic DNA through upregulation of SIRT6 and TETs
... Although studies have revealed altered DNA methyla- tion patterns in pancreatic islets, adipose tissue, and skel- etal muscle [5, 13, 27 – 30], it remains poorly understood that the dynamic regulation of 5hmC in ...
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Location of the 5-Methylcytosine Group on the Bacteriophage φX174 Genome
... In this paper, we report the results of restriction enzyme digestions of the methyl-labeled viral DNA, after its conversion to the replicative form by in vitro synthesis in the presence [r] ...
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Focused, high accuracy 5-methylcytosine quantitation with base resolution by benchtop next-generation sequencing
... (0, 5, 10, 25, 50, 75 and 100%) were ana- lyzed for CpG methylation levels using BSAS (Figure 1) as well as the traditional Sanger sequencing approach with epigenetic sequencing methylation analysis ...from ...
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Distinct 5 methylcytosine profiles in poly(A) RNA from mouse embryonic stem cells and brain
... In addition to these modifications, it has been known since the 1970s that the C5 atom of cytosine can be a target of methylation in poly(A) RNA in HeLa and ham- ster cells [24, 25]. By contrast, other early studies ...
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Human cytomegalovirus-induced DNA polymerase and its interaction with the triphosphates of 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)-5-methyluracil, -5-iodocytosine, and -5-methylcytosine.
... The data from this study and those from a previous report 20 reveal that the inhibitory effects of FMAUTP, FMACTP, and FIACTP on HCMV-induced DNA polymerase correlate with the antiviral [r] ...
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DNA methylation in the hippocampus in rodent and primate models of aging and Alzheimer’s disease
... With the aging of the world’s population, Alzheimer’s disease (AD) is a growing problem. It is caused by pathological hallmarks including amyloid β plaques and neurofibrillary tangles, but the underlying molecular ...
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