5'-nucleotidase
Acidosis during ischemia promotes adenosine triphosphate resynthesis in postischemic rat heart In vivo regulation of 5' nucleotidase
... cytosolic 5'- nucleotidase (5'-NT): AMP-->adenosine plus inorganic phosphate ...of 5'-NT, we used the tools of 31P nuclear magnetic resonance ...
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Ecto 5′ Nucleotidase Activity in Human T Cell Subsets DECREASED NUMBERS OF ECTO 5′ NUCLEOTIDASE POSITIVE CELLS FROM BOTH OKT4+ AND OKT8+ CELLS IN PATIENTS WITH HYPOGAMMAGLOBULINEMIA
... T lymphocytes from control subjects were separated into subsets using monoclonal antibodies of the OKT series and complement lysis and analyzed for ecto-5¢-nucleotidase activity both by quantitative ...
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Elevated 5' nucleotidase and alkaline phosphodiesterase activities in trout gill localize to endothelial (pillar) cells
... No prior studies concerning APD in fish gill could be identified. Trout gill plasma membrane APD activity exhibited a divalent cation requirement and substrate preference consistent with those of APD characterized from ...
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Regulatory processes in the central nervous system: A study of g-protein abundance and of soluble 5'-nucleotidase
... It is generally appreciated that at least four distinct isoforms of 5'-nucleotidase exist (see Zimmerman, 1992 for review). One form is an ectoenzyme which is believed to facilitate production of ...
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Alpha 1 adrenoceptor activation mediates the infarct size limiting effect of ischemic preconditioning through augmentation of 5' nucleotidase activity
... increasing 5'- nucleotidase ...of 5'- nucleotidase ...for 5 min separated by 5 min of reperfusion (ischemic preconditioning) in 82 ...cytosolic 5'-nucleotidase ...
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Adenosine formed by 5′ nucleotidase mediates tubuloglomerular feedback
... of 5′-nucleotidase or adenosine in normal function of the proximal ...by 5′-nucleotidase might be necessary for normal glomerular tubular ...(Figure 5, center ...
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Evidence for deactivation of both ectosolic and cytosolic 5' nucleotidase by adenosine A1 receptor activation in the rat cardiomyocytes
... affects 5'-nucleotidase ...cytosolic 5'-nucleotidase activity was decreased by exogenous adenosine (ectosolic 5'-nucleotidase activity, ...cytosolic 5'-nucleotidase ...
7
Synthesis of Silver Nanoparticles from Malva parviflora Extract and Effect on Ecto-5'- Nucleotidase(5'-NT), ADA and AMPDA Enzymes in Sera of Patients with Arthrosclerosis Ahlam M. Farhan| Rasha A. Jassim| Nafeesa J. Kadhim| Wesen Adel Mehdi | Atheer Awad Mehde
... Determination of Ag NPs on the sera adenosine aminohydrolase (ADA), AMP-aminohydrolase activities and Ecto-5'-nucleotidase activity. The effect of Ag NPs on the levels of adenosine aminohydrolase (ADA), ...
9
Serum 5’-Nucleotidase in Neonatal Hepatitis and Biliary Atresia: Preliminary Observations
... Serum 5’-nucleotidase levels in infants with neonatal hepatitis and extrahepatic biliary atresia... 814 NEPHROSIS diagnostic reliability of the serum 5’-nucleo- tidase level in neonatal [r] ...
5
Ecto 5′ nucleotidase (CD73) regulation by hypoxia inducible factor 1 mediates permeability changes in intestinal epithelia
... Under conditions of limited oxygen availability (hypoxia), multiple cell types release adenine nucleotides in the form of ATP, ADP, and AMP. Extracellular AMP is metabolized to adenosine by surface-expressed ...
11
Human lymphocytes: 5' nucleotidase positive and negative subpopulations
... enzyme, 5'-nucleotidase (5'N) ...decreased 5'N value in CLL represents a lower activity per cell or fewer enzyme-containing cells than in the normal, conditions were established for the ...
5
Lead poisoning: association with hemolytic anemia, basophilic stippling, erythrocyte pyrimidine 5' nucleotidase deficiency, and intraerythrocytic accumulation of pyrimidines
... Lead intoxication is accompanied by an acquired deficiency of erythrocyte pryimidine- specific, 5'-nucleotidase. Genetically determined deficiency of this enzyme is associated with chronic hemolysis, marked ...
8
Heterogeneity of 5′ Nucleotidase Activity in Lymphocytes in Chronic Lymphocytic Leukemia
... of 5¢-nucleotidase was determined in lymphocyte plasma membranes from 14 normal subjects and 10 patients with chronic lymphocytic leukemia ...detectable 5¢-nucleotidase ...
5
Effects of low level lead exposure on pyrimidine 5' nucleotidase and other erythrocyte enzymes Possible role of pyrimidine 5' nucleotidase in the pathogenesis of lead induced anemia
... demonstrated that significant inhibition of pyrimidine 5'-nucleotidase occurred in the presence of lead at concentrations that had minimal effects on many other erythrocyte enzymes assayed simultaneously. ...
7
Methotrexate and sulfasalazine promote adenosine release by a mechanism that requires ecto 5' nucleotidase mediated conversion of adenine nucleotides
... Another more likely explanation for the methotrexate- and sulfasalazine-mediated increase in extracellular adenosine is that methotrexate and sulfasalazine modulate purine nucle- otide metabolism, and thereby promote the ...
7
Insights into thymic purine metabolism and adenosine deaminase deficiency revealed by transgenic mice overexpressing ecto 5' nucleotidase (CD73)
... with 5 9 -NT activity virtually absent in cortical thymocytes and increasing in medullary ...of 5 9 -NT in thymocyte development is not well defined, and the reason for the regulated expression of this ...
9
Characterization of recombinant mouse ecto-5'-nucleotidase
... Given that two subtypes of adenosine activated purinergic receptors were localized to the spinal cord (Choca et al., 1987; Geiger et al., 1984; Goodman and Synder, 1982), the investiga[r] ...
110
Early events after infection of Escherichia coli by bacteriophage T5. II. Control of the bacteriophage-induced 5'-nucleotidase activity.
... The presence of an actual protein-caused inactivation in Al-infected cells has been further substantiated by noting that when chloramphenicol is added to Al-infected cells before 6 min a[r] ...
9
Oligomeric interface modulation causes misregulation of purine 5´-nucleotidase in relapsed leukemia
... Helix A is a crucial region for cN-II allosteric regula- tion. Importantly, our study provided novel insight into its behavior. Structural comparison of wild-type cN-II and the hyperactive mutants revealed that helix A ...
16
Characterization and properties of ecto-5' nucleotidase in white adipose tissue
... 5 'Nase in a 0 . 5M sodium bicarbonate buffer, pH 9 . A and left overnight for maximal binding. Unbound protein was removed the next day by at least three washes with a 0.9%(w/v) NaCl, 0.1%(v/v) Tween 20 solution. ...
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