7-AAD
Garlic compounds selectively kill childhood pre-B acute lymphoblastic leukemia cells in vitro without reducing T-cell function: Potential therapeutic use in the treatment of ALL
... At concentrations of garlic compounds that resulted in signifi - cant apoptosis of ALL cells but not of T-cells as determined by Annexin V and 7-AAD staining, there was no signifi cant change in CD25 and CD69 ...
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Nortropane alkaloids as pharmacological chaperones in the rescue of equine adipose-derived mesenchymal stromal stem cells affected by metabolic syndrome through mitochondrial potentiation, endoplasmic reticulum stress mitigation and insulin resistance alleviation
... 7-AAD: 7-Aminoactinomycine D; AAPH: α , α′ -Azodiisobutyramidine dihydrochloride; ABTS: 2,2 ′ -Azino-bis3-ethylbenzothiazoline-6-sulphonic acid; ADIPOQ: Adiponectin; AKT: Protein kinase B; AMPK: 5 ′ ...
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CYLD suppression enhances the pro inflammatory effects and hyperproliferation of rheumatoid arthritis fibroblast like synoviocytes by enhancing NF κB activation
... joints; 7-AAD: 7-aminoactinomycin D; AA: Adjuvant-induced arthritis; ACPA: Anti- cyclic citrullinated peptide antibody; ACR: American College of Rheumatology; ANOVA: Aanalysis of variance; BCA: ...
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Tumor necrosis factor alpha promotes survival in methotrexate exposed macrophages by an NF kappaB dependent pathway
... The Recombinant Macrophage-Colony Stimulating Fac- tor (M-CSF) was obtained from R&D Systems (Sydney, NSW, Australia). Annexin V conjugated to phycoery- thrin (Annexin V-PE) and 7-amino-actinomycin ...
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Combination therapies enhance immunoregulatory properties of MIAMI cells
... 7-AAD: 7-Aminoactinomycin D; Ad-MSCs: Adipose-derived MSCs; BM- MSCs: Bone marrow MSCs; CFSE: Carboxylfluorescein succinimidyl ester; CQ: Chloroquine; DMEM: Dulbecco ’ s modified Eagle ’ s medium; ...
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Increased serum levels of fractalkine and mobilisation of CD34+CD45− endothelial progenitor cells in systemic sclerosis
... and 7-aminoactinomycin D (7-AAD) (Stem-Kit Reagents; Beckman Coulter Life Sciences), according to the manu- facturer’s ...within 7-AAD- negative viable cells displaying forward scatter ...
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Activation status of mucosal associated invariant T cells reflects disease activity and pathology of systemic lupus erythematosus
... Peripheral blood mononuclear cells (PBMCs) were puri- fied from heparinized blood by centrifugation over a Ficoll-Paque gradient (GE Healthcare, Uppsala, Sweden). PBMCs were incubated with Fc receptor blocking re- agent ...
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Methotrexate inhibits effects of platelet derived growth factor and interleukin 1β on rheumatoid arthritis fibroblast like synoviocytes
... Abbreviations 7-AAD: 7-Amino-actinomycin D; ANOVA: Analysis of variance; CDKI: Cyclindependent kinase inhibitor; DNMT: DNA methyltransferase; FBS: Fetal bovine serum; FLS: Fibroblast-lik[r] ...
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Intracellular free radical production by peripheral blood T lymphocytes from patients with systemic sclerosis: role of NADPH oxidase and ERK1/2
... 7-AAD: 7-aminoactinomycin D; BrdU: bromodeoxyuridine; conA: concanavalin A; DCFH-DA: 2 ′ , 7 ′ -dichlorodihydrofluorescin diacetate; DPI: diphenylene iodonium; FCS: fetal calf serum; FITC: ...
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Silencing of hepatic fate-conversion factors induce tumorigenesis in reprogrammed hepatic progenitor-like cells
... Adherent cells were washed with PBS and detached by treatment with trypsin (Life Technologies). Cells fixed with 4 % paraformaldehyde for 10 min at 4 °C were permeabilized with 90 % methanol for 30 min at 4 °C. After ...
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Monocytes are essential for inhibition of synovial T cell glucocorticoid mediated apoptosis in rheumatoid arthritis
... = 7), triamci- nolone (n = 4), or methylprednisolone (n = ...and 7-amino-actinomycin D (7-AAD) as specified by the manufacturer (559763; BD Biosciences) and analyzed by flow ...
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Peripheral blood natural killer cell percentages in granulomatosis with polyangiitis correlate with disease inactivity and stage
... 7-AAD: 7-aminoactinomycin; ANCA: antineutrophil cytoplasmic antibody; AUC: area under the curve; B7-H6: member of the B7 family of immunoreceptors, ligand for NKp30; BVAS: Birmingham Vasculitis ...
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The Cytotoxic Effect of Cecropin A and Cecropin B on the MDA MB 231 and M14K Tumour Cell Lines
... This assay was used to detect and measure the apoptotic process. Apoptosis is characterized by cell morphology changes, nuclear condensation, pyknosis, and also by a series of biochemical events causing mitochondrial ...
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Interleukin-22 is increased in multiple sclerosis patients and targets astrocytes
... 7-AAD: 7-aminoactinomycin D; AM: astrocyte medium; AQP4: aquaporin-4; BSA: bovine serum albumin; Cav-1: caveolin-1; CFS: cerebrospinal fluid; CFSE: carboxyfluorescein succinimidyl ester; CIS: ...
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Nano-Pulse Stimulation is a physical modality that can trigger immunogenic tumor cell death
... own laboratory by assaying the levels of activated caspases 3/7 as well as measuring the percentage of cells undergo- ing the stages of apoptotic cell death through the presence of cell surface phosphatidylserine ...
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The NEDD8-activating enzyme inhibitor MLN4924 induces G2 arrest and apoptosis in T-cell acute lymphoblastic leukemia
... altered genes, pathway analysis revealed that 9 ribosome genes, 4 steroid biosynthesis genes, and 7 hematopoietic cell lineage genes were enriched (Figure 7A). Aberrant expression of ribosomal proteins frequently ...
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ENDORSER CREDIBILITY EFFECTS ON ALGERIAN CONSUMER’S ATTITUDES TOWARDS ADVERTISING AND PURCHASE INTENTION: THE MEDIATING ROLE OF ATTITUDE TOWARD BRAND
... Licensed under Creative Common Page 98 advertising effects that are assumed to happen through the flow of casual relationship between (Aad-Ab), (Aad-PI), and (Ab-PI). The advertising effect models can be ...
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Case Report Acute agitated delirium and death after burr hole drainage for chronic subdural hematoma: a case report
... of AAD by paying very close attention to the perioperative examination and management, especially the following details: (1) increase awareness of potential CSDH postoperative accidents; (2) give moderate ...
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PAMS - Piping and Component Analysis Aad Monitoring System Application and Visualisation
... The system is meant to contain all information necessary to perform most of the standard analyses and monitor piping systems and components for an existing and operating plant [7]. At present most of the piping ...
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Purposing AAL to an Aging Population : AAW (Walking), AAD (Driving) and the IoV (Internet of Vehicles)
... the AAD systems of some of the cars in the platoon, it can easily cause incredible havoc by forcing incorrect behaviour and wrong responses in these ...dynamic AAD assessment ...
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