A phagocytophilum
Anaplasma marginale and A phagocytophilum in cattle in Tunisia
... The specificity of the duplex PCR was confirmed by se- quencing PCR amplicons of A. marginale and A. pha- gocytophilum using primers M4-OvMar-F/M4-Mar-R for msp4 gene and Msp2-3 F/Msp2-3R for msp2, respect- ively. ...
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Detection of Anaplasma marginale and A phagocytophilum in Bovine Peripheral Blood Samples by Duplex Real Time Reverse Transcriptase PCR Assay
... A. phagocytophilum have been shown to have identical 16S rRNA gene sequences, but this sequence differs consider- ably among closely related Anaplasma species (7, ...
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Serological and molecular prevalence of canine vector borne diseases (CVBDs) in Korea
... Results: Serological survey of 532 outdoor dogs revealed the highest prevalence for Dirofilaria immitis (25.2%), followed by Anaplasma phagocytophilum (15.6%), Ehrlichia canis (4.7%) whereas Borrelia burgdorferi ...
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Melting pot of tick borne zoonoses: the European hedgehog contributes to the maintenance of various tick borne diseases in natural cycles urban and suburban areas
... All ticks were processed individually. Nucleic acids were extracted using the DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany), according to the manufacturer’s in- structions. The extracted DNA was stored at -20 ...
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Transmission of Anaplasma phagocytophilum (Foggie, 1949) by Ixodes ricinus (Linnaeus, 1758) ticks feeding on dogs and artificial membranes
... The A. phagocytophilum strain used (“TIBA strain”) was isolated in June 2015 from a clinical case (dog) in Ter- schelling, the Netherlands. Amplification of the ank gene was performed as described by Massung et ...
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Mitogenic Component in Polar Lipid-Enriched Anaplasma phagocytophilum Membranes
... A. phagocytophilum virulence is mediated in part by inducing host innate immunopathology (8, 9, 18, 27, ...A. phagocytophilum surface-exposed protein Msp2, is probably not an important stimulus for tissue ...
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Serological and molecular detection of Anaplasma phagocytophilum in horses reared in Korea
... A. phagocytophilum, two different nested PCRs (nPCR) were performed using the EE1/EE2 and EE3/EE4 primers, which amplify a 928-bp fragment (Barlough et ...A. phagocytophilum DNA isolated from dog blood was ...
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Fröhlich, Julia (2017): Genotyping of Anaplasma phagocytophilum in natural endemic cycles. Dissertation, LMU München: Tierärztliche Fakultät
... A. phagocytophilum ist der Erreger für die Granulozytäre Anaplasmose, einer immer häufiger auftretende Krankheit bei Mensch und ...A. phagocytophilum unter natürlichen Lebensbedingungen und seiner ...
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Serologic Cross-Reactivity between Anaplasma marginale and Anaplasma phagocytophilum
... The sheep examined in the present study originated from and were kept in Great Britain, free of A. marginale and A. ovis. And finally, the Brown Swiss cattle came from tick-free regions of Switzerland and samples ...
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Anaplasma phagocytophilum evolves in geographical and biotic niches of vertebrates and ticks
... Haplotypes recorded in South Korea were also independ- ent of the complete network. The disconnection of this group of South Korean haplotypes seems to be derived from the fact that they are associated with different ...
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Delineating anaplasma phagocytophilum ecotypes in coexisting, discrete enzootic cycles
... A. phagocytophilum has a wide geographic distribution, and numerous members of the Ixodes genus have been impli- cated in its ...A. phagocytophilum in the laboratory has been reported (29) and, ...
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Innate Immune Response to Anaplasma phagocytophilum Contributes to Hepatic Injury
... A. phagocytophilum infection, inflammatory signaling in C3H mice (i) proceeds via a MyD88- independent pathway and (ii) involves alternative TLRs or unidentified ligands differentially expressed between C3H and B6 ...
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Detection of Anaplasma phagocytophilum, Babesia microti, Borrelia burgdorferi, Borrelia miyamotoi, and Powassan Virus in Ticks by a Multiplex Real-Time Reverse Transcription-PCR Assay
... Anaplasma phagocytophilum, the agent of human granulocytic anaplas- mosis, Babesia microti, the agent of babesiosis, Borrelia miyamotoi, and the deer tick virus clade of Powassan virus (POWV) ...A. ...
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Spatial and seasonal variation in the prevalence of Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in questing Ixodes ricinus ticks in Norway
... in Leipzig, Germany [61], but lower than the 20.5% reported in Spain [62]. Our estimate of prevalence of A. phagocytophilum was higher than the 4.5% reported in an earlier study pooling various areas distributed ...
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Molecular detection of Anaplasma phagocytophilum in carrier cattle of Iran - first documented report
... A. phagocytophilum have been detected in human and confirmed by serological and molecular methods ...A. phagocytophilum was reported in Korea (19) and the first Molecular detection of ...A. ...
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Sequential Analysis of Anaplasma phagocytophilum msp2 Transcription in Murine and Equine Models of Human Granulocytic Anaplasmosis
... Anaplasma phagocytophilum causes human granulocytic anaplasmosis by inducing immunopathologic re- ...A. phagocytophilum in vivo, with specific Msp2 expression triggering immunopathologic injury or clinical ...
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In Situ Detection of Anaplasma spp by DNA Target Primed Rolling Circle Amplification of a Padlock Probe and Intracellular Colocalization with Immunofluorescently Labeled Host Cell von Willebrand Factor
... A. phagocytophilum rolling-circle amplification product and von Willebrand factor ...A. phagocytophilum can be contin- uously cultivated in endothelial cells (12) and preliminary im- munofluorescent ...
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Zoonotic pathogens associated with Hyalomma aegyptium in endangered tortoises: evidence for host switching behaviour in ticks?
... Anaplasma phagocytophilum, the agent of human gran- ulocytic anaplasmosis, is vectored in Europe by Ixodes ricinus and can infect a wide range of domestic and wild vertebrate hosts, including rodents, horses, dogs ...
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Detection of selected pathogens in ticks collected from cats and dogs in the Wrocław Agglomeration, South West Poland
... The prevalence of pathogens differed between the tick species. Ixodes ricinus individuals were the most often infected species. The lowest infection levels were ob- served in I. hexagonus ticks. From all tested pathogens ...
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Anaplasma phagocytophilum prevalence in ticks and rodents in an urban and natural habitat in South Western Slovakia
... A. phagocytophilum-infected ticks in Bratislava Forest Park, compared to the natural habitat in Fúgelka, could be partly affected by higher population densities of roe deer and hedgehogs which are known to carry ...
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