Acanthamoeba castellanii
In Vitro Activity of Squaramides and Acyclic Polyamine Derivatives against Trophozoites and Cysts of Acanthamoeba castellanii
... In this study, we have evaluated the effectiveness of several squaramides and acyclic polyamine compounds of new synthesis against trophozoites and cysts of Acanthamoeba castellanii Neff strain. ...
14
Cytotoxic effects of mercury, cadmium, lead and zinc on acanthamoeba castellanii
... on Acanthamoeba castellanii was conducted in order to understand more about their toxicity on the amoeba by looking at their EC 50 and effect on the amoeba cell ...
5
Acanthamoeba castellanii Promotion of In Vitro Survival and Transmission of Coxsackie B3 Viruses
... whether Acanthamoeba could play a role in the survival and transmission of coxsackieviruses and focused on in vitro interactions between Acanthamoeba castellanii and coxsackie B3 viruses ...data, ...
7
Visualizing the interaction of Acanthamoeba castellanii with human retinal epithelial cells by spontaneous Raman and CARS imaging
... by Acanthamoeba castellanii infection, there are still many gaps in our understanding of the mechanisms that drive the interaction between this organism and host ...A. castellanii trophozoites access ...
34
Evidence that hsp90 Is Involved in the Altered Interactions of Acanthamoeba castellanii Variants with Bacteria
... There are many similarities between the interactions of environmental protozoa with pathogenic bacterial species and those observed in mammalian macrophages. Since single-celled protozoa predate mammalian hosts, it is ...
12
Induced encystment improves resistance to preservation and storage of Acanthamoeba castellanii
... viable Acanthamoeba castellanii organisms were ...A. castellanii are naturally capable of encystment, studies were undertaken to determine whether induced encystment might improve the viability of ...
5
Comparative in vitro effectiveness of a novel contact lens multipurpose solution on Acanthamoeba castellanii
... cells/mL Acanthamoeba castellanii cultures were commixed with 100 μL of either commercial or novel MPS solution with contact times ranging from 1 to 96 h before centrifuga- tion for 10 min at 1000 ...
7
PNA FISH assay uncovers Enterococcus faecium as a facultative intracellular bacterium in co-culture with Acanthamoeba castellanii
... endocarditis. Acanthamoeba castellanii is an aquatic environmental eukaryote that is considered as an ideal cell in studying eukaryote- prokaryote interaction and as a powerful tool for the culture of some ...
9
Antiparasitic Efficacy of Artemisia ludoviciana Nutt. (Asteraceae) Essential Oil for Acanthamoeba castellanii, Leishmania infantum and Trichomonas vaginalis
... Background: Artemisia ludoviciana Nutt. (Asteraceae) is an aromatic, herbaceous, perennial plant and known commonly name as “White Sage”, “Black Sage”, “Prairie Sage” or “Cudweed Sagewort”. It is traditionally used as an ...
10
The Osmoregulatory System of the Amoeba, Acanthamoeba Castellanii
... Effect of polyethylene glycol in the external medium on the rate of output of the contractile vacuole of the amoeba Acanthamoeba castellanii Neff cultured in i % MP Rate of vacuolar outp[r] ...
22
Major Role for Cysteine Proteases during the Early Phase of Acanthamoeba castellanii Encystment
... Acanthamoeba castellanii is a facultative pathogen that has a two-stage life cycle comprising the vegetatively growing trophozoite stage and the dormant cyst ...the Acanthamoeba en- cystment process ...
8
The Transcriptional Response of Cryptococcus neoformans to Ingestion by Acanthamoeba castellanii and Macrophages Provides Insights into the Evolutionary Adaptation to the Mammalian Host
... Virulence of Cryptococcus neoformans for mammals, and in particular its intracellular style, was proposed to emerge from evo- lutionary pressures on its natural environment by protozoan predation, which promoted the ...
14
Anti-amoeba Effect of Branched Medium Chain Fatty Acids and Their Salts on Acanthamoeba Castellanii - Volume 10 Number 4 (Aug. 2019) - IJCEA
... Abstract—Acanthamoeba castellanii is an organism that causes Acanthamoeba keratitis, an infectious ...disease. Acanthamoeba are protists that generally inhabiting soils, freshwater, and sea ...
5
Acanthamoeba castellanii of the T4 genotype is a potential environmental host for Enterobacter aerogenes and Aeromonas hydrophila
... the family of Enterobacteriaceae, is often associated with nosocomial infections [5]. The colonization of E. aerogenes most commonly occurs amongst intensive care unit (ICU) patients, usually in the respiratory, urinary, ...
8
Molecular and biochemical characterization of key enzymes in the cysteine and serine metabolic pathways of Acanthamoeba castellanii
... A. castellanii RNA was extracted from trophozo- ites using RNeasy® Plus Mini Kit (Qiagen, Hilden, Germany), and complementary DNA (cDNA) was syn- thesised using a PrimeScript® 1st strand cDNA synthesis kit ...
10
The effect of surface treatment of silicone hydrogel contact lenses on the attachment of Acanthamoeba castellanii trophozoites
... amoebae from the surface of the plates. The PAS containing the trophozoites was centrifuged at 3000 rpm for 10 minutes at room temperature, the supernatant removed, and the Acanthamoeba pellet resuspended in 10 ml ...
15
Use of In Vitro Assays To Determine Effects of Human Serum on Biological Characteristics of Acanthamoeba castellanii
... of Acanthamoeba phagocytosis was determined as follows: number of ...on Acanthamoeba phagocytosis, we performed phagocytosis assays with fluorescein isothiocyanate (FITC)-labeled, heat- killed ...enhances ...
6
Isolation of two strains of Acanthamoeba castellanii from human tissue and their pathogenicity and isoenzyme profiles
... Two strains of amoebae, one CDC:0180: 1 from the lung tissue of a patient who died of granulomatous amoebic encephalitis and the other CDC:0179:1 from the debrided tissue of a mandibular[r] ...
8
Environmental adaptation of Acanthamoeba castellanii and Entamoeba histolytica at genome level as seen by comparative genomic analysis
... Orthology groups expanded in A. castellanii TolA like proteins have eighteen paralogs in Ac and four in Eh, but their functions have not been yet characterized in Amoebozoa. The majority of Ac paralogs are ...
15
Abnormalities of Endocytosis, Phagocytosis, and Development Process in Dictyostelium Cells That Over-Express Acanthamoeba castellanii Metacaspase Protein.
... A. castellanii has a metacaspase type-1 (Acmcp) that is highly ex- pressed during the encystation process ...including Acanthamoeba (7) and Naegleria ...A. castellanii metacaspase has the most ...
17